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The map label for this gene is proP1 [H]

Identifier: 148360156

GI number: 148360156

Start: 2884275

End: 2885507

Strand: Reverse

Name: proP1 [H]

Synonym: LPC_2087

Alternate gene names: 148360156

Gene position: 2885507-2884275 (Counterclockwise)

Preceding gene: 148360155

Following gene: 148360157

Centisome position: 80.68

GC content: 38.12

Gene sequence:

>1233_bases
ATGGATAAAAAAATTATTGCGGCAGCCTCTTTTGGCACCTTGTTTGAAATGTACGACTATGCCATTTATGGGTTTATGGC
GCCCATTTTGGCGCCTCTTTTTTTTCCTGCCACTGATAAACACACTGCTCTCATCGCAACATTTGGTATTTTTGCCGCAG
GTTTTCTTGTTCGGCCATTCTCTGCTTATTGGTTAGGGAATCTGGGTGACAAAATTGGCCGCAAAAAAACATTGACTATG
ACTCTTATGGTCATGACTTTTTCATCAAGTTTTATTGGTTTATTACCTACCTATCAAGAGGCGGGCATTCTCGCTCCGAT
ATTACTCACGCTATGTCGATTAACTCAAGGGGTTTCTGTAGCAGGGGAAATGACACTAGCCAGTATTTACATTTATGAAA
ATGTGAACACGCGTCATAAAGGTTTAGCAGTCAGTTTCGTCACTTTAGGTTCTATTTGTGGATTGTTGCTTGCAATAATA
GTAAGCAAAATAACCCTGATATTTTTTTCCAGTCAGTCTCCCTATTTTCTCTGGCGATTCCCATTTTTAATAGGAATATT
CGTAGGTTTTATCGGACTTTATTTGCGAAGTCAAACCAGAGAATTAACCGATTTTGCAAAAGTAATTGTTCAAAGACAAT
CTCATCCCATACTGAATGCCTGGCGTTATCGGAAAAAAGCAATTGCTGCTATTTTTCTTTTGGCAATTTATTGCAACAGT
AGCTTTTATACATTTTTTGTTTATATGCCTTCATACCTGCAACAGCAGGAGTTGCCATTAACTACAGCGATGGATGTCAT
TATCATAGGGATGACTTGTTATAGCATGGGTCTCTTATTATCAGGTTTCACCTCTGATATTTTAGGCAGGCGCTTTCCTT
TGGCAGTCAGTTTAATTGCAACTTTATGTACCGTTTTGGTAATAAATTATTTGATCAATACAGGTACTTTATTACAAATT
ACTGTCGCCTGCAGTGTTTTGGCTGTCATTATCTCTTTTTTTATTGGAAGCGCATTTACCGCCAGCCTTGAACAATTACC
GCAGGAAGAGCGCGCCAGTGGCTTTACTTTATTTTTTAACTTATCTAATTCCCTATTTGGAGGCACAGCACCACTCATTA
TTTTGCTATTAAGTTATGCTTTCATCCATTTTGCATTTTCTATTTATTTGATGGTGATGGCTTTGTTGACTCTTCCTGCC
CTATGGTTGATTCAAGAAAAAGGCACGAATTAA

Upstream 100 bases:

>100_bases
CCAGTCAAGGATGAAGTGGATTCAGAGAATCAAGCTCAGAAATCAATAGAGTTAAAGTAAGCTGTATTACTACCTGTGAA
ACAAAAGCAACAGCTTGAAC

Downstream 100 bases:

>100_bases
TTTATTAGTTTATGGTACGAAAAACAGTGATCTAACACAAAAATGTGCCCAGTTTGGTTGCGTAAAATATATTTATCCTG
ACTGTGGACATACGGAAGTT

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MDKKIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPATDKHTALIATFGIFAAGFLVRPFSAYWLGNLGDKIGRKKTLTM
TLMVMTFSSSFIGLLPTYQEAGILAPILLTLCRLTQGVSVAGEMTLASIYIYENVNTRHKGLAVSFVTLGSICGLLLAII
VSKITLIFFSSQSPYFLWRFPFLIGIFVGFIGLYLRSQTRELTDFAKVIVQRQSHPILNAWRYRKKAIAAIFLLAIYCNS
SFYTFFVYMPSYLQQQELPLTTAMDVIIIGMTCYSMGLLLSGFTSDILGRRFPLAVSLIATLCTVLVINYLINTGTLLQI
TVACSVLAVIISFFIGSAFTASLEQLPQEERASGFTLFFNLSNSLFGGTAPLIILLLSYAFIHFAFSIYLMVMALLTLPA
LWLIQEKGTN

Sequences:

>Translated_410_residues
MDKKIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPATDKHTALIATFGIFAAGFLVRPFSAYWLGNLGDKIGRKKTLTM
TLMVMTFSSSFIGLLPTYQEAGILAPILLTLCRLTQGVSVAGEMTLASIYIYENVNTRHKGLAVSFVTLGSICGLLLAII
VSKITLIFFSSQSPYFLWRFPFLIGIFVGFIGLYLRSQTRELTDFAKVIVQRQSHPILNAWRYRKKAIAAIFLLAIYCNS
SFYTFFVYMPSYLQQQELPLTTAMDVIIIGMTCYSMGLLLSGFTSDILGRRFPLAVSLIATLCTVLVINYLINTGTLLQI
TVACSVLAVIISFFIGSAFTASLEQLPQEERASGFTLFFNLSNSLFGGTAPLIILLLSYAFIHFAFSIYLMVMALLTLPA
LWLIQEKGTN
>Mature_410_residues
MDKKIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPATDKHTALIATFGIFAAGFLVRPFSAYWLGNLGDKIGRKKTLTM
TLMVMTFSSSFIGLLPTYQEAGILAPILLTLCRLTQGVSVAGEMTLASIYIYENVNTRHKGLAVSFVTLGSICGLLLAII
VSKITLIFFSSQSPYFLWRFPFLIGIFVGFIGLYLRSQTRELTDFAKVIVQRQSHPILNAWRYRKKAIAAIFLLAIYCNS
SFYTFFVYMPSYLQQQELPLTTAMDVIIIGMTCYSMGLLLSGFTSDILGRRFPLAVSLIATLCTVLVINYLINTGTLLQI
TVACSVLAVIISFFIGSAFTASLEQLPQEERASGFTLFFNLSNSLFGGTAPLIILLLSYAFIHFAFSIYLMVMALLTLPA
LWLIQEKGTN

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=412, Percent_Identity=33.0097087378641, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1788942, Length=412, Percent_Identity=31.7961165048544, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1789941, Length=416, Percent_Identity=28.8461538461538, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1788292, Length=294, Percent_Identity=28.5714285714286, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87082404, Length=314, Percent_Identity=22.6114649681529, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI87082231, Length=466, Percent_Identity=22.7467811158798, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45431; Mature: 45431

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPATDKHTALIATFGIFAAGFLVRPF
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCH
SAYWLGNLGDKIGRKKTLTMTLMVMTFSSSFIGLLPTYQEAGILAPILLTLCRLTQGVSV
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHCCCCHHHCCHHHHHHHHHHHHHCCCCH
AGEMTLASIYIYENVNTRHKGLAVSFVTLGSICGLLLAIIVSKITLIFFSSQSPYFLWRF
HCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEHHH
PFLIGIFVGFIGLYLRSQTRELTDFAKVIVQRQSHPILNAWRYRKKAIAAIFLLAIYCNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
SFYTFFVYMPSYLQQQELPLTTAMDVIIIGMTCYSMGLLLSGFTSDILGRRFPLAVSLIA
CCEEHEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TLCTVLVINYLINTGTLLQITVACSVLAVIISFFIGSAFTASLEQLPQEERASGFTLFFN
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEEE
LSNSLFGGTAPLIILLLSYAFIHFAFSIYLMVMALLTLPALWLIQEKGTN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDKKIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPATDKHTALIATFGIFAAGFLVRPF
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCH
SAYWLGNLGDKIGRKKTLTMTLMVMTFSSSFIGLLPTYQEAGILAPILLTLCRLTQGVSV
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHCCCCHHHCCHHHHHHHHHHHHHCCCCH
AGEMTLASIYIYENVNTRHKGLAVSFVTLGSICGLLLAIIVSKITLIFFSSQSPYFLWRF
HCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEHHH
PFLIGIFVGFIGLYLRSQTRELTDFAKVIVQRQSHPILNAWRYRKKAIAAIFLLAIYCNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
SFYTFFVYMPSYLQQQELPLTTAMDVIIIGMTCYSMGLLLSGFTSDILGRRFPLAVSLIA
CCEEHEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TLCTVLVINYLINTGTLLQITVACSVLAVIISFFIGSAFTASLEQLPQEERASGFTLFFN
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEEE
LSNSLFGGTAPLIILLLSYAFIHFAFSIYLMVMALLTLPALWLIQEKGTN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]