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The map label for this gene is sflR [H]

Identifier: 148360108

GI number: 148360108

Start: 2934629

End: 2935819

Strand: Reverse

Name: sflR [H]

Synonym: LPC_2039

Alternate gene names: 148360108

Gene position: 2935819-2934629 (Counterclockwise)

Preceding gene: 148360106

Following gene: 148360120

Centisome position: 82.09

GC content: 44.16

Gene sequence:

>1191_bases
TTGAATTTTATTGGCTGGACAGAAAGACAAAGTCTTTTTCGATTATTTCCGCTCATTATCAGCATTTCTTTTGCTATGGA
TGTTTTTGTGCCCGCAATTCCGGAAATGAGTCGTTTTTTCAAGACTGACAGTACAACGATGCAAGCTACTCTTTATTTGT
TTATGCTCACTGTAGCTTTGGGGCAGTTATTGGTTGGACCTTTGGCCGATCACTTTGGACGAAGGCAGATGGCCTTGAGT
ATGGCACTTCTCTTTTTTGCAGGATCGTTGATCTCTGCGTTGGCTCCGTCAGTACCGGCATTGATTTTTGCACGTATCAT
TCAGGCAGCTGGGGCCTGTGGAACCTATTTACTGTGTTTTATTATTGTTCGAGATAATTTTTCAACGGGCACCTGTGCCC
GCTTGTTTAGTATCTTGTCTGGGACGAATTCAATGGTTGCCAGTTCTGCTCCAGTCATTGGGGGATTATTGTTGGATGTG
ACGCAAGATTGGCATAGTGGATTCTACTTTTTAACCTTGCTTGGGTTATTAATGAGTGGCATAGCCTTTCGTTACATCCC
CGATTATCACTATCCCAAACCTGAGCTGCCCCAGATGAGGGTGTACCAAGTGATGAGGCAGATAATAAGTAACTCTGACT
TTCGCCAATACACATTGGTTGCATCAGCCAGTTTGCTGGGATTGTACTTGTTTTGTGCTCAATCACCAGGTATTTTGATC
ACACAACTGCACCTGAGTGCGACTGAATATGGTTTCTGGTTTGGTTTGAATGCAATGGCTGTGTTTGTTGCCAATATGAT
GGCCGCACGACTTACTTATTCTCACTCATTGGAAAGCATAGTGCGTTGGGGCTTGGTTTTAATGACACTGTCATGCTTTT
TTATGCTGGCTCTTAATCTTCGTCAATGCAGCATTTTAAGTTTTATGCTTCCCATGCTTTGCTTGACTGTGGGTATTGGT
ATTAGCATGGGCACAGCAACAGCGCTTGCTTTAAAGGATTTTGAACAGCAAGCTGGTGTCGCTACCGCTCTGTTGGGTGC
TTGCCAGTTTGGTCTCGCGGGTCTGATTGGTGTTTTGACAGCTCAATGGATGCCCGGGCCATTAAGTGTAGCTATCCCCG
TACTTTGTTTCACTGCAGTGGGATTGGCTAGAATCACCAAATTCAGTTTTAGAATATTGAGCGGCTCTTAA

Upstream 100 bases:

>100_bases
TTGATTTATAAGGTTACTTTGTAATCGTCCGTTTAAATCTGATACAATTAAGCGCTATTTGTATTTTTCATAAGTAATAT
TTTAACAGGAACACCATTAA

Downstream 100 bases:

>100_bases
TCTTGTCCGCAGGCAATTGGCCAAAAAAGACCGATTACCGGGTATGCGGTAGGTAACACTGTAACCTTCAGTGTTGTTTA
TCCTGCATGTGGTTCTGTAT

Product: florfenicol efflux pump

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MNFIGWTERQSLFRLFPLIISISFAMDVFVPAIPEMSRFFKTDSTTMQATLYLFMLTVALGQLLVGPLADHFGRRQMALS
MALLFFAGSLISALAPSVPALIFARIIQAAGACGTYLLCFIIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDV
TQDWHSGFYFLTLLGLLMSGIAFRYIPDYHYPKPELPQMRVYQVMRQIISNSDFRQYTLVASASLLGLYLFCAQSPGILI
TQLHLSATEYGFWFGLNAMAVFVANMMAARLTYSHSLESIVRWGLVLMTLSCFFMLALNLRQCSILSFMLPMLCLTVGIG
ISMGTATALALKDFEQQAGVATALLGACQFGLAGLIGVLTAQWMPGPLSVAIPVLCFTAVGLARITKFSFRILSGS

Sequences:

>Translated_396_residues
MNFIGWTERQSLFRLFPLIISISFAMDVFVPAIPEMSRFFKTDSTTMQATLYLFMLTVALGQLLVGPLADHFGRRQMALS
MALLFFAGSLISALAPSVPALIFARIIQAAGACGTYLLCFIIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDV
TQDWHSGFYFLTLLGLLMSGIAFRYIPDYHYPKPELPQMRVYQVMRQIISNSDFRQYTLVASASLLGLYLFCAQSPGILI
TQLHLSATEYGFWFGLNAMAVFVANMMAARLTYSHSLESIVRWGLVLMTLSCFFMLALNLRQCSILSFMLPMLCLTVGIG
ISMGTATALALKDFEQQAGVATALLGACQFGLAGLIGVLTAQWMPGPLSVAIPVLCFTAVGLARITKFSFRILSGS
>Mature_396_residues
MNFIGWTERQSLFRLFPLIISISFAMDVFVPAIPEMSRFFKTDSTTMQATLYLFMLTVALGQLLVGPLADHFGRRQMALS
MALLFFAGSLISALAPSVPALIFARIIQAAGACGTYLLCFIIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDV
TQDWHSGFYFLTLLGLLMSGIAFRYIPDYHYPKPELPQMRVYQVMRQIISNSDFRQYTLVASASLLGLYLFCAQSPGILI
TQLHLSATEYGFWFGLNAMAVFVANMMAARLTYSHSLESIVRWGLVLMTLSCFFMLALNLRQCSILSFMLPMLCLTVGIG
ISMGTATALALKDFEQQAGVATALLGACQFGLAGLIGVLTAQWMPGPLSVAIPVLCFTAVGLARITKFSFRILSGS

Specific function: Appears to provoke a reduction of the content of the major porins ompA and ompC [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=342, Percent_Identity=27.1929824561404, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87082312, Length=343, Percent_Identity=25.9475218658892, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI48994889, Length=362, Percent_Identity=23.2044198895028, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790146, Length=341, Percent_Identity=22.2873900293255, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43206; Mature: 43206

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFIGWTERQSLFRLFPLIISISFAMDVFVPAIPEMSRFFKTDSTTMQATLYLFMLTVAL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHH
GQLLVGPLADHFGRRQMALSMALLFFAGSLISALAPSVPALIFARIIQAAGACGTYLLCF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
IIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDVTQDWHSGFYFLTLLGLLMSG
HHHCCCCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IAFRYIPDYHYPKPELPQMRVYQVMRQIISNSDFRQYTLVASASLLGLYLFCAQSPGILI
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
TQLHLSATEYGFWFGLNAMAVFVANMMAARLTYSHSLESIVRWGLVLMTLSCFFMLALNL
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQCSILSFMLPMLCLTVGIGISMGTATALALKDFEQQAGVATALLGACQFGLAGLIGVLT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQWMPGPLSVAIPVLCFTAVGLARITKFSFRILSGS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MNFIGWTERQSLFRLFPLIISISFAMDVFVPAIPEMSRFFKTDSTTMQATLYLFMLTVAL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHH
GQLLVGPLADHFGRRQMALSMALLFFAGSLISALAPSVPALIFARIIQAAGACGTYLLCF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
IIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDVTQDWHSGFYFLTLLGLLMSG
HHHCCCCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IAFRYIPDYHYPKPELPQMRVYQVMRQIISNSDFRQYTLVASASLLGLYLFCAQSPGILI
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
TQLHLSATEYGFWFGLNAMAVFVANMMAARLTYSHSLESIVRWGLVLMTLSCFFMLALNL
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQCSILSFMLPMLCLTVGIGISMGTATALALKDFEQQAGVATALLGACQFGLAGLIGVLT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQWMPGPLSVAIPVLCFTAVGLARITKFSFRILSGS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1648560; 1658833 [H]