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The map label for this gene is trmL [H]

Identifier: 148360047

GI number: 148360047

Start: 2991778

End: 2992245

Strand: Direct

Name: trmL [H]

Synonym: LPC_1978

Alternate gene names: 148360047

Gene position: 2991778-2992245 (Clockwise)

Preceding gene: 148360049

Following gene: 148360044

Centisome position: 83.65

GC content: 39.74

Gene sequence:

>468_bases
ATGCTACTGGAAAATCACATGATCCATATTGCGCTTTATCAACCAGAAATTCCACCGAACACGGGCAATATTATTCGACT
CTGCGCCAATAGTGGAGCTCAACTGCATTTGATTCACCCATTAGGCTTTACTTTGGAAGATAAACAAATGCGACGAGCAG
GTCTTGATTATCATCAATGGGCCTCCATATTGCATTATGAGAGTTGGCAAACATTCTTGCAGTCTCATTCACAACGACGA
TTATTTTGCTGTAGCGCAAAAGGAAAAGTGAAGTATAGTGACGTCGATTATAAGGCAGATGACATCCTATTATTTGGACC
CGAATCTTGCGGCTTACCTCAACAAATACTGAACCAATATTCAACAATACGGATTCCCATGAGAGAAAATAATCGTAGTC
TCAACTTATCAAATGCTGTAGCAATCATATTGTACGAAGCATGGAGGCAAATGGATTTCAACATGTAA

Upstream 100 bases:

>100_bases
GACATATCAACTCCCTGCTCGATTTTTAAAGTATGTAGTAAAATTGCATCCTGCCACTCTATTCCTGGCTTCGAATAAAT
CAATTAAAATGATAGCCAGA

Downstream 100 bases:

>100_bases
GCATTAACCTGGTAATCCACCTGATAGCGATTACAGCAATGAAAGAGACAGGTTATGCAAGCAGTACTCTAATATAAACA
GGACTTACGAAAAACCACAA

Product: TrmH family transporter RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MLLENHMIHIALYQPEIPPNTGNIIRLCANSGAQLHLIHPLGFTLEDKQMRRAGLDYHQWASILHYESWQTFLQSHSQRR
LFCCSAKGKVKYSDVDYKADDILLFGPESCGLPQQILNQYSTIRIPMRENNRSLNLSNAVAIILYEAWRQMDFNM

Sequences:

>Translated_155_residues
MLLENHMIHIALYQPEIPPNTGNIIRLCANSGAQLHLIHPLGFTLEDKQMRRAGLDYHQWASILHYESWQTFLQSHSQRR
LFCCSAKGKVKYSDVDYKADDILLFGPESCGLPQQILNQYSTIRIPMRENNRSLNLSNAVAIILYEAWRQMDFNM
>Mature_155_residues
MLLENHMIHIALYQPEIPPNTGNIIRLCANSGAQLHLIHPLGFTLEDKQMRRAGLDYHQWASILHYESWQTFLQSHSQRR
LFCCSAKGKVKYSDVDYKADDILLFGPESCGLPQQILNQYSTIRIPMRENNRSLNLSNAVAIILYEAWRQMDFNM

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=150, Percent_Identity=51.3333333333333, Blast_Score=177, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17992; Mature: 17992

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLENHMIHIALYQPEIPPNTGNIIRLCANSGAQLHLIHPLGFTLEDKQMRRAGLDYHQW
CCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHCCCHHHH
ASILHYESWQTFLQSHSQRRLFCCSAKGKVKYSDVDYKADDILLFGPESCGLPQQILNQY
HHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCEEEECCCCCCCHHHHHCCH
STIRIPMRENNRSLNLSNAVAIILYEAWRQMDFNM
HEEEEEECCCCCEEECCHHEEHEEEHHHHHHCCCC
>Mature Secondary Structure
MLLENHMIHIALYQPEIPPNTGNIIRLCANSGAQLHLIHPLGFTLEDKQMRRAGLDYHQW
CCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHCCCHHHH
ASILHYESWQTFLQSHSQRRLFCCSAKGKVKYSDVDYKADDILLFGPESCGLPQQILNQY
HHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCEEEECCCCCCCHHHHHCCH
STIRIPMRENNRSLNLSNAVAIILYEAWRQMDFNM
HEEEEEECCCCCEEECCHHEEHEEEHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA