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The map label for this gene is proP [H]

Identifier: 148359906

GI number: 148359906

Start: 2783826

End: 2785100

Strand: Reverse

Name: proP [H]

Synonym: LPC_1835

Alternate gene names: 148359906

Gene position: 2785100-2783826 (Counterclockwise)

Preceding gene: 148359907

Following gene: 148359905

Centisome position: 77.87

GC content: 37.8

Gene sequence:

>1275_bases
ATGAACATATCCTCTTCGAGTAAAGCATTATTCGCTGGAGTTTCTGGCACGGCATTACAATGGTATGACTTTGCACTTTT
TGGATATTTCGCTCCAATTATTGCAGCAACTTATTTTCCTAATGACAATCAATTTGCTTCGCTTTTAAGTGCTTTTGGAG
TATTTGCTGTTGGTTATTTATTAGCGCCAATAGGTTCACTTTTTTTTGGCTATATTGGTGATCAGTTCGGCAGGAAGCGT
GCTTTAACTCTTAGTATTTTAGCAATGGCGATCCCTACAGCTATGATCAGTATTGTTCCTTCTTACCAATATATTGGAAT
TACAGCGCCTCTTCTTATTACTTTGTTGAGGATAATTCAAGGTTTTGTCGCAAGTAGCGAATTTACTGGATCCGCTATTT
TTCTTGTTGAGCATGCTAAGACTGAGAAAAAAGCATTGTATGGTTGTTTAACCAGTTCGGCTTACAGCACTGGTTTGATA
CTAGCAGGCTTAGCTGCTTCATTCTTTACGGCCTCATTTATGCCAGACTGGGGATGGCGAATCGGTTTTGGATTGGCTTT
GATTGCTGGCATTTTGATTTTTTATTTACGAACCCATGTTGCTGAAACACCTGAATATGAAAATATTGTTCAACATGATA
AACGACGCCTGCCATTTCTAGCTGCGCTAAAAGAAGCGCCTTTGGCAGTAGTTGGTATTATAGGGATAGCATGGTTAGTC
AGTATAATGACTTTTGGAACCTATGTATTTACAGCCACATACCTGCATAGCTATTTTCATATTTCACTTGGTTTAGCTAC
ATTAATTATCACCATAGCTTTAGCAGTTGATGCCACACTTGAACCATTTATTGCTTTGTTAGCAGGCAGAATAGGGTTAT
TAAAAGTAATTCGATTGGGTATGGTTGCTATGTTACTTTTAAGTATTCCCATTTTTTTCCTACTTGCCACTGGGAATGTT
GTTTTGATTGCACTAGGATTAGTTTTCATGTCTATTTTGATTGCCATTACTTATGCGCCACTCAACGCATATATGGTTAT
TCTATTTCCTCATCAATATCGTTATAGTGGGTTTGGAGTCGCTTTCAATGTAGGCATTTCATTGTTTGGTGGTACAACTC
CTATAGTGATGATGTGGCTAGTGAATTCAACAAGTAATTTTATTTCACCTGCTTGGTATTACATGTTTGGCGCAATCATA
GGGTTAGCCTCAATAATGGTTTGTGAACGAGGTCGAAGAAGATTGATACGTTTGGAATCTACTTTAGCCTATTAA

Upstream 100 bases:

>100_bases
CATTTTTATAAAAAAATTGGTGGTGTCGCTTTATTAGACTGGCAAATTTTTAAATGTGACCAAATTGGTATTAATAACTT
GATGAGCATAGGAGTTGCTA

Downstream 100 bases:

>100_bases
AAATGAGGTATTTAAGATGTTGTTATTTAGTTCTGCTTTAGCAAAACACAAGCTTACTTTTACACGATTAAGTGATTGCA
TGGAATATCTTCCCCTTGCA

Product: proline/betaine transport protein like protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MNISSSSKALFAGVSGTALQWYDFALFGYFAPIIAATYFPNDNQFASLLSAFGVFAVGYLLAPIGSLFFGYIGDQFGRKR
ALTLSILAMAIPTAMISIVPSYQYIGITAPLLITLLRIIQGFVASSEFTGSAIFLVEHAKTEKKALYGCLTSSAYSTGLI
LAGLAASFFTASFMPDWGWRIGFGLALIAGILIFYLRTHVAETPEYENIVQHDKRRLPFLAALKEAPLAVVGIIGIAWLV
SIMTFGTYVFTATYLHSYFHISLGLATLIITIALAVDATLEPFIALLAGRIGLLKVIRLGMVAMLLLSIPIFFLLATGNV
VLIALGLVFMSILIAITYAPLNAYMVILFPHQYRYSGFGVAFNVGISLFGGTTPIVMMWLVNSTSNFISPAWYYMFGAII
GLASIMVCERGRRRLIRLESTLAY

Sequences:

>Translated_424_residues
MNISSSSKALFAGVSGTALQWYDFALFGYFAPIIAATYFPNDNQFASLLSAFGVFAVGYLLAPIGSLFFGYIGDQFGRKR
ALTLSILAMAIPTAMISIVPSYQYIGITAPLLITLLRIIQGFVASSEFTGSAIFLVEHAKTEKKALYGCLTSSAYSTGLI
LAGLAASFFTASFMPDWGWRIGFGLALIAGILIFYLRTHVAETPEYENIVQHDKRRLPFLAALKEAPLAVVGIIGIAWLV
SIMTFGTYVFTATYLHSYFHISLGLATLIITIALAVDATLEPFIALLAGRIGLLKVIRLGMVAMLLLSIPIFFLLATGNV
VLIALGLVFMSILIAITYAPLNAYMVILFPHQYRYSGFGVAFNVGISLFGGTTPIVMMWLVNSTSNFISPAWYYMFGAII
GLASIMVCERGRRRLIRLESTLAY
>Mature_424_residues
MNISSSSKALFAGVSGTALQWYDFALFGYFAPIIAATYFPNDNQFASLLSAFGVFAVGYLLAPIGSLFFGYIGDQFGRKR
ALTLSILAMAIPTAMISIVPSYQYIGITAPLLITLLRIIQGFVASSEFTGSAIFLVEHAKTEKKALYGCLTSSAYSTGLI
LAGLAASFFTASFMPDWGWRIGFGLALIAGILIFYLRTHVAETPEYENIVQHDKRRLPFLAALKEAPLAVVGIIGIAWLV
SIMTFGTYVFTATYLHSYFHISLGLATLIITIALAVDATLEPFIALLAGRIGLLKVIRLGMVAMLLLSIPIFFLLATGNV
VLIALGLVFMSILIAITYAPLNAYMVILFPHQYRYSGFGVAFNVGISLFGGTTPIVMMWLVNSTSNFISPAWYYMFGAII
GLASIMVCERGRRRLIRLESTLAY

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=428, Percent_Identity=32.4766355140187, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788292, Length=431, Percent_Identity=31.7865429234339, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1788942, Length=411, Percent_Identity=29.4403892944039, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1789941, Length=396, Percent_Identity=30.3030303030303, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI87082404, Length=367, Percent_Identity=25.3405994550409, Blast_Score=85, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46136; Mature: 46136

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISSSSKALFAGVSGTALQWYDFALFGYFAPIIAATYFPNDNQFASLLSAFGVFAVGYL
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LAPIGSLFFGYIGDQFGRKRALTLSILAMAIPTAMISIVPSYQYIGITAPLLITLLRIIQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHH
GFVASSEFTGSAIFLVEHAKTEKKALYGCLTSSAYSTGLILAGLAASFFTASFMPDWGWR
HHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
IGFGLALIAGILIFYLRTHVAETPEYENIVQHDKRRLPFLAALKEAPLAVVGIIGIAWLV
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHH
SIMTFGTYVFTATYLHSYFHISLGLATLIITIALAVDATLEPFIALLAGRIGLLKVIRLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MVAMLLLSIPIFFLLATGNVVLIALGLVFMSILIAITYAPLNAYMVILFPHQYRYSGFGV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCE
AFNVGISLFGGTTPIVMMWLVNSTSNFISPAWYYMFGAIIGLASIMVCERGRRRLIRLES
EEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLAY
HHCC
>Mature Secondary Structure
MNISSSSKALFAGVSGTALQWYDFALFGYFAPIIAATYFPNDNQFASLLSAFGVFAVGYL
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LAPIGSLFFGYIGDQFGRKRALTLSILAMAIPTAMISIVPSYQYIGITAPLLITLLRIIQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHH
GFVASSEFTGSAIFLVEHAKTEKKALYGCLTSSAYSTGLILAGLAASFFTASFMPDWGWR
HHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
IGFGLALIAGILIFYLRTHVAETPEYENIVQHDKRRLPFLAALKEAPLAVVGIIGIAWLV
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHH
SIMTFGTYVFTATYLHSYFHISLGLATLIITIALAVDATLEPFIALLAGRIGLLKVIRLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MVAMLLLSIPIFFLLATGNVVLIALGLVFMSILIAITYAPLNAYMVILFPHQYRYSGFGV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCE
AFNVGISLFGGTTPIVMMWLVNSTSNFISPAWYYMFGAIIGLASIMVCERGRRRLIRLES
EEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLAY
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]