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The map label for this gene is rpoD [H]

Identifier: 148359903

GI number: 148359903

Start: 2779608

End: 2781467

Strand: Direct

Name: rpoD [H]

Synonym: LPC_1831

Alternate gene names: 148359903

Gene position: 2779608-2781467 (Clockwise)

Preceding gene: 148359902

Following gene: 148359908

Centisome position: 77.72

GC content: 42.26

Gene sequence:

>1860_bases
ATGAATGACCAAGATCAGCAAAGCTCACAAATTACGAAAGTCATTAGCCTGGGTAAAGAGCAAGGTTATTTGACTTACAG
TCAAATTAATGACTTATTACCTAATATTGTGGATACAGAGCATTTTGATGTAATCATTAGCATGCTGGAAGGCATGAACA
TCAAAGTTTTTGAGCTTCCACCCAGTGAAGATGAACTTGCGCTCTTATTAAATACTGAGGAAGTCCCAGATATTGAAGAA
GCAGCCATGGTACTCGCCTCTGTGGATAAAGAAACAGGTAGAACAACTGACCCGGTTCGTATGTATATGCGCGAAATGGG
CACAGTAGAACTGCTGACTCGTGAAGGTGAAATCCGCATTGCCAAACGCATTGAAGAAGGCATTTACCAGGTTCTAAAAT
CCCTGGCGCATTATCCGGAAACAGTGCAACTGGTTCTTGAAGACTATGATCGCTTCCTTGCAGAAGAAATTCGCCTTAAT
GAAATCATTAGTGGTTTTGCTGATGTTGATGAAGAAATAGCACCTGCCACTAACATTGGTTCAATGCTGGATGAAGAGCA
GCAGGAAGAAATACTTCTTGATGTGGATGAAGAAGATGAAGACGGCGAAGGCGGCATATCTGATGTGGATGATGGACCCA
ATCCTGAACAAGCAAAAGTTTACTTTGATGAACTGCGAGAAGCCTTTGAAAAGGCAATGAAAGCCCTCAAGGAACATGGC
CGTACGAATAAAAAAACAGTCGAGCTGCTCGATTCCATGTCAGAATCTTTTCTGAAACTCAAATTGACTTCCAGACAAGT
TGATAAATTAACTCGTCATTTTCGCCAATTGCGAAATCATATCCGTGAATTTGAGAGGAGCATTATGCGTCTTTGCATCG
AAAAAGCACGCATCCCCAGAAAATTGTTTATAGATACCTTCCCAGGTCAGGAAACAGATCTAAGCTGGTTAGAAACCATT
ATCAGCAAACATGCCAAAAAACTTGATCTTCAGCGTATAGAAGAAAATCGGGAAGAAATACTACGCTTACAATCCAAGCT
GGCCTCTTTTGAAATCGAGTATGGTTTAACCATTAGTGAAATCAAAGACATTAACCGCAAGATGTCTATTGGCGAAGCCA
AAGCAAGACGTGCTAAAAAAGAAATGGTCGAGGCTAACTTGCGTCTTGTGATTTCCATCGCCAAAAAATACACCAACCGC
GGCTTGCAGTTTCTTGATTTGATCCAGGAAGGCAACATTGGCTTGATGAAAGCAGTCGATAAGTTCGAATACCGCCGTGG
CTATAAGTTTTCTACCTATGCGACCTGGTGGATTAGACAAGCGATTACACGATCCATAGCCGATCAGGCGCGGACTATTC
GTATTCCGGTTCATATGATTGAAACCATCAATAAGCTCAATCGTATTTCTCGCCAAATTTTGCAAGAAACTGGCCGGGAA
GCGACACCGGAGGAATTGGCGGAAAAAATGGAATTAAGTGAAGATAAAATTCGCAAAGTTCTAAAGATAGCCAAAGAACC
TATTTCCATGGAAACCCCTGTCGGTGATGATGACGACTCTCATCTGGGTGATTTTATTGAAGACAACAACATTGAATCGC
CAATCGATATGGCTACAGCGGAAGGTTTAAGAGAAGCCACTTTAGAAATACTGGAAACCCTGACCCCACGAGAAGCCAAG
GTTCTGCGTATGCGTTTTGGTATCGAAATGAATACAGACCATACCCTGGAAGAAGTTGGTAAACAATTTGACGTAACCCG
CGAACGTATTCGCCAAATCGAAGCAAAAGCACTACGCAAATTGCGCCACCCTTCACGTTCAGAAAAACTAAGAAGCTTCC
TTGAAGGGGATGAAGGTTAA

Upstream 100 bases:

>100_bases
AAAAATAAAAATACTGAAATACTGGTATGTTGCAGCTACCCAGTTTATAATTTAAAACTTTTTGTAATTTCCCATTGTTC
CAACAGAAGTGCCTATGACC

Downstream 100 bases:

>100_bases
TTTAATGACTGTTTATATAAACAGTCATTAAAAAAATATAAAAAATAGTAGAACATTAAAGAAATTAAGTTTAAAATGAC
CCCCTCTCCGGGCCCATAGC

Product: RNA polymerase sigma 70 factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MNDQDQQSSQITKVISLGKEQGYLTYSQINDLLPNIVDTEHFDVIISMLEGMNIKVFELPPSEDELALLLNTEEVPDIEE
AAMVLASVDKETGRTTDPVRMYMREMGTVELLTREGEIRIAKRIEEGIYQVLKSLAHYPETVQLVLEDYDRFLAEEIRLN
EIISGFADVDEEIAPATNIGSMLDEEQQEEILLDVDEEDEDGEGGISDVDDGPNPEQAKVYFDELREAFEKAMKALKEHG
RTNKKTVELLDSMSESFLKLKLTSRQVDKLTRHFRQLRNHIREFERSIMRLCIEKARIPRKLFIDTFPGQETDLSWLETI
ISKHAKKLDLQRIEENREEILRLQSKLASFEIEYGLTISEIKDINRKMSIGEAKARRAKKEMVEANLRLVISIAKKYTNR
GLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQILQETGRE
ATPEELAEKMELSEDKIRKVLKIAKEPISMETPVGDDDDSHLGDFIEDNNIESPIDMATAEGLREATLEILETLTPREAK
VLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEKLRSFLEGDEG

Sequences:

>Translated_619_residues
MNDQDQQSSQITKVISLGKEQGYLTYSQINDLLPNIVDTEHFDVIISMLEGMNIKVFELPPSEDELALLLNTEEVPDIEE
AAMVLASVDKETGRTTDPVRMYMREMGTVELLTREGEIRIAKRIEEGIYQVLKSLAHYPETVQLVLEDYDRFLAEEIRLN
EIISGFADVDEEIAPATNIGSMLDEEQQEEILLDVDEEDEDGEGGISDVDDGPNPEQAKVYFDELREAFEKAMKALKEHG
RTNKKTVELLDSMSESFLKLKLTSRQVDKLTRHFRQLRNHIREFERSIMRLCIEKARIPRKLFIDTFPGQETDLSWLETI
ISKHAKKLDLQRIEENREEILRLQSKLASFEIEYGLTISEIKDINRKMSIGEAKARRAKKEMVEANLRLVISIAKKYTNR
GLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQILQETGRE
ATPEELAEKMELSEDKIRKVLKIAKEPISMETPVGDDDDSHLGDFIEDNNIESPIDMATAEGLREATLEILETLTPREAK
VLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEKLRSFLEGDEG
>Mature_619_residues
MNDQDQQSSQITKVISLGKEQGYLTYSQINDLLPNIVDTEHFDVIISMLEGMNIKVFELPPSEDELALLLNTEEVPDIEE
AAMVLASVDKETGRTTDPVRMYMREMGTVELLTREGEIRIAKRIEEGIYQVLKSLAHYPETVQLVLEDYDRFLAEEIRLN
EIISGFADVDEEIAPATNIGSMLDEEQQEEILLDVDEEDEDGEGGISDVDDGPNPEQAKVYFDELREAFEKAMKALKEHG
RTNKKTVELLDSMSESFLKLKLTSRQVDKLTRHFRQLRNHIREFERSIMRLCIEKARIPRKLFIDTFPGQETDLSWLETI
ISKHAKKLDLQRIEENREEILRLQSKLASFEIEYGLTISEIKDINRKMSIGEAKARRAKKEMVEANLRLVISIAKKYTNR
GLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQILQETGRE
ATPEELAEKMELSEDKIRKVLKIAKEPISMETPVGDDDDSHLGDFIEDNNIESPIDMATAEGLREATLEILETLTPREAK
VLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEKLRSFLEGDEG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=611, Percent_Identity=63.1751227495908, Blast_Score=709, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=268, Percent_Identity=39.1791044776119, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=28.9256198347107, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788231, Length=212, Percent_Identity=29.7169811320755, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 71476; Mature: 71476

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDQDQQSSQITKVISLGKEQGYLTYSQINDLLPNIVDTEHFDVIISMLEGMNIKVFELP
CCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECC
PSEDELALLLNTEEVPDIEEAAMVLASVDKETGRTTDPVRMYMREMGTVELLTREGEIRI
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHEEECCCCHHH
AKRIEEGIYQVLKSLAHYPETVQLVLEDYDRFLAEEIRLNEIISGFADVDEEIAPATNIG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SMLDEEQQEEILLDVDEEDEDGEGGISDVDDGPNPEQAKVYFDELREAFEKAMKALKEHG
HHHCHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
RTNKKTVELLDSMSESFLKLKLTSRQVDKLTRHFRQLRNHIREFERSIMRLCIEKARIPR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
KLFIDTFPGQETDLSWLETIISKHAKKLDLQRIEENREEILRLQSKLASFEIEYGLTISE
HHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHCCCCHHH
IKDINRKMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHH
KFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQILQETGRE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
ATPEELAEKMELSEDKIRKVLKIAKEPISMETPVGDDDDSHLGDFIEDNNIESPIDMATA
CCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHH
EGLREATLEILETLTPREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRK
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRHPSRSEKLRSFLEGDEG
HHCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MNDQDQQSSQITKVISLGKEQGYLTYSQINDLLPNIVDTEHFDVIISMLEGMNIKVFELP
CCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECC
PSEDELALLLNTEEVPDIEEAAMVLASVDKETGRTTDPVRMYMREMGTVELLTREGEIRI
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHEEECCCCHHH
AKRIEEGIYQVLKSLAHYPETVQLVLEDYDRFLAEEIRLNEIISGFADVDEEIAPATNIG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SMLDEEQQEEILLDVDEEDEDGEGGISDVDDGPNPEQAKVYFDELREAFEKAMKALKEHG
HHHCHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
RTNKKTVELLDSMSESFLKLKLTSRQVDKLTRHFRQLRNHIREFERSIMRLCIEKARIPR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
KLFIDTFPGQETDLSWLETIISKHAKKLDLQRIEENREEILRLQSKLASFEIEYGLTISE
HHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHCCCCHHH
IKDINRKMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHH
KFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQILQETGRE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
ATPEELAEKMELSEDKIRKVLKIAKEPISMETPVGDDDDSHLGDFIEDNNIESPIDMATA
CCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHH
EGLREATLEILETLTPREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRK
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRHPSRSEKLRSFLEGDEG
HHCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]