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The map label for this gene is gatA1 [H]

Identifier: 148359896

GI number: 148359896

Start: 2772911

End: 2774320

Strand: Reverse

Name: gatA1 [H]

Synonym: LPC_1824

Alternate gene names: 148359896

Gene position: 2774320-2772911 (Counterclockwise)

Preceding gene: 148359898

Following gene: 148359894

Centisome position: 77.57

GC content: 44.4

Gene sequence:

>1410_bases
ATGGATGCCATTCATCTCCTTTCTGCAACAGAAATCATTCGCCGCATTAAAATGAAAGAACTCTCGGCTGCAGAGGTCAT
GGTGGCGCACTTAAACCATATTGATAAGATTAATCCTGTAATCAATGCGCTCACCGAACGTATTCCCCCTGAGGAATGCT
TAAAGCAGGCAAAGGAAATTGACAAGAGTATCGCTTCGAAAAAAAATCTGAACAAGTTAATGGGATTGCCTGTTGCCATA
AAAGACGCTTTGTATGTGAAAGGACTAATTTGTTCTTCGGCATGTTCAGGATTTTATAAGGGAGAAAAAGCAGCACGTGA
TGCGACCCTGGTTTCTCGACTGAAAAAGGAAGGAGCAATTATTCTTGGCTTAACCAATGTTCCTGAATTGTGTCGCGGTG
GTGATTCGGATAATTTAATCTATGGACGGACCAATAACCCTTATGATCTGGCAAGAACCAGTGGAGGCAGTAGTGGTGGT
TCCGCGGCTTTGATCGCTGCAGGAGGCGTTCCTTTTGCTTTGGGTTCCGATGGTGGAGGTAGTTTAATGCAGCCTGCCCA
TTGTTGCGGTATTGTTGCATTGAAGCCAACACATGGCCATCTTCCTCATACAGGCTCTGTAGGTGGGGATTCCTATGGTC
TTATTGGCAATCTGATTTCTTTTGGACCAATGGCTCGTTCTGTCAGCGACCTTCGTTTAGGACTCTCCGTGCTTGCGGGC
TCTGACCAATATGATCCTTATACAAATCCAGTTCCTGTTATGCCTGCTGCACCACTAAAAAAATTACGTGTAGCCTATTT
TACAGAGAATGGTTTTACGCCAGTTGATGTGGAGATACAGAACGTTGTTAAATCAGCAGCCTTGGCTTTGCAGGATGATG
TGGCTATAGTGAGGGAGGTGCGACCTGATTGTGTCAGCAAGGCTTTTGATTTGCACTGGGAGCTTTTTCTCGGGGGCGAT
TGCGGAGCGGGATTTAAAACTATGTTATCGGAGTTAGGGGTTAACAATCTGTCATGGGAATTACAAGAGTTCTTGCGTCA
AGCGGAACAAACTCAATTCTCGGTGACCCAATTACATCAACGTATGCGCGAAATCGATTTGTTTCGTCTGGAATTAGCTT
TATTTATGCAAGATTATGATGTATTGATTAGCCCAGTGTTTCCAACAGTAGCAAAACCACATGGGATTGGGATTAAAGAA
ATCAGCGATTTTTCCTATGCGATGACACATAATTTAAGTGGATTTCCAACGATCAGCCTAAGATGTGGGGCTTCAGCCGA
GGGGTTGCCCATTAATATATTAATAGCCGCTAATCGCTGGAAAGATACTACCTCTCTGGCTGTTGCAGAACGCCTTGAGC
AATTAATGGGGGGATATAAACCCCCTTCTCTAATCCACTCTCTGCAATGA

Upstream 100 bases:

>100_bases
TTTGCAATAGTTTGAGACGGATACTGAATGTTGCTTTTTAGTCCTGTTCGGTGTGTCACTATCATTCTACTCCATTATTT
AAGGAAATTTTACTAAAGCT

Downstream 100 bases:

>100_bases
AGCAAGAATCAATAATTTTTGAAACAGCGAATTAGGGAAGAAACAAGGCTTGCAAATCATTAGTATATTGCCTGCCTAAT
GGTGTTACTTGCCAATCAGT

Product: amidase

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A 1 [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MDAIHLLSATEIIRRIKMKELSAAEVMVAHLNHIDKINPVINALTERIPPEECLKQAKEIDKSIASKKNLNKLMGLPVAI
KDALYVKGLICSSACSGFYKGEKAARDATLVSRLKKEGAIILGLTNVPELCRGGDSDNLIYGRTNNPYDLARTSGGSSGG
SAALIAAGGVPFALGSDGGGSLMQPAHCCGIVALKPTHGHLPHTGSVGGDSYGLIGNLISFGPMARSVSDLRLGLSVLAG
SDQYDPYTNPVPVMPAAPLKKLRVAYFTENGFTPVDVEIQNVVKSAALALQDDVAIVREVRPDCVSKAFDLHWELFLGGD
CGAGFKTMLSELGVNNLSWELQEFLRQAEQTQFSVTQLHQRMREIDLFRLELALFMQDYDVLISPVFPTVAKPHGIGIKE
ISDFSYAMTHNLSGFPTISLRCGASAEGLPINILIAANRWKDTTSLAVAERLEQLMGGYKPPSLIHSLQ

Sequences:

>Translated_469_residues
MDAIHLLSATEIIRRIKMKELSAAEVMVAHLNHIDKINPVINALTERIPPEECLKQAKEIDKSIASKKNLNKLMGLPVAI
KDALYVKGLICSSACSGFYKGEKAARDATLVSRLKKEGAIILGLTNVPELCRGGDSDNLIYGRTNNPYDLARTSGGSSGG
SAALIAAGGVPFALGSDGGGSLMQPAHCCGIVALKPTHGHLPHTGSVGGDSYGLIGNLISFGPMARSVSDLRLGLSVLAG
SDQYDPYTNPVPVMPAAPLKKLRVAYFTENGFTPVDVEIQNVVKSAALALQDDVAIVREVRPDCVSKAFDLHWELFLGGD
CGAGFKTMLSELGVNNLSWELQEFLRQAEQTQFSVTQLHQRMREIDLFRLELALFMQDYDVLISPVFPTVAKPHGIGIKE
ISDFSYAMTHNLSGFPTISLRCGASAEGLPINILIAANRWKDTTSLAVAERLEQLMGGYKPPSLIHSLQ
>Mature_469_residues
MDAIHLLSATEIIRRIKMKELSAAEVMVAHLNHIDKINPVINALTERIPPEECLKQAKEIDKSIASKKNLNKLMGLPVAI
KDALYVKGLICSSACSGFYKGEKAARDATLVSRLKKEGAIILGLTNVPELCRGGDSDNLIYGRTNNPYDLARTSGGSSGG
SAALIAAGGVPFALGSDGGGSLMQPAHCCGIVALKPTHGHLPHTGSVGGDSYGLIGNLISFGPMARSVSDLRLGLSVLAG
SDQYDPYTNPVPVMPAAPLKKLRVAYFTENGFTPVDVEIQNVVKSAALALQDDVAIVREVRPDCVSKAFDLHWELFLGGD
CGAGFKTMLSELGVNNLSWELQEFLRQAEQTQFSVTQLHQRMREIDLFRLELALFMQDYDVLISPVFPTVAKPHGIGIKE
ISDFSYAMTHNLSGFPTISLRCGASAEGLPINILIAANRWKDTTSLAVAERLEQLMGGYKPPSLIHSLQ

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=494, Percent_Identity=30.3643724696356, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI222831590, Length=508, Percent_Identity=24.8031496062992, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI166795287, Length=507, Percent_Identity=26.0355029585799, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=503, Percent_Identity=29.4234592445328, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17556264, Length=306, Percent_Identity=31.6993464052288, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17538252, Length=488, Percent_Identity=27.0491803278689, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71990152, Length=479, Percent_Identity=26.0960334029228, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17556276, Length=313, Percent_Identity=33.2268370607029, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17556278, Length=313, Percent_Identity=33.2268370607029, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17538254, Length=451, Percent_Identity=28.8248337028825, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32565337, Length=304, Percent_Identity=25, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=161, Percent_Identity=34.7826086956522, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320448, Length=242, Percent_Identity=34.297520661157, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319685, Length=227, Percent_Identity=34.3612334801762, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=188, Percent_Identity=33.5106382978723, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24652985, Length=497, Percent_Identity=30.1810865191147, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI19922090, Length=497, Percent_Identity=30.1810865191147, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24652981, Length=497, Percent_Identity=30.1810865191147, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24652983, Length=497, Percent_Identity=30.1810865191147, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI21356731, Length=482, Percent_Identity=30.0829875518672, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24648435, Length=491, Percent_Identity=28.5132382892057, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24648437, Length=491, Percent_Identity=28.5132382892057, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI45550774, Length=491, Percent_Identity=28.5132382892057, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI161078093, Length=486, Percent_Identity=29.4238683127572, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24644968, Length=506, Percent_Identity=26.6798418972332, Blast_Score=148, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24648441, Length=423, Percent_Identity=27.8959810874704, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24648439, Length=423, Percent_Identity=27.8959810874704, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24648113, Length=219, Percent_Identity=36.5296803652968, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 50492; Mature: 50492

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAIHLLSATEIIRRIKMKELSAAEVMVAHLNHIDKINPVINALTERIPPEECLKQAKEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DKSIASKKNLNKLMGLPVAIKDALYVKGLICSSACSGFYKGEKAARDATLVSRLKKEGAI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCE
ILGLTNVPELCRGGDSDNLIYGRTNNPYDLARTSGGSSGGSAALIAAGGVPFALGSDGGG
EEEECCCHHHHCCCCCCCEEECCCCCCHHHHCCCCCCCCCCEEEEEECCCCEEECCCCCC
SLMQPAHCCGIVALKPTHGHLPHTGSVGGDSYGLIGNLISFGPMARSVSDLRLGLSVLAG
CCCCCHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SDQYDPYTNPVPVMPAAPLKKLRVAYFTENGFTPVDVEIQNVVKSAALALQDDVAIVREV
CCCCCCCCCCCCCCCCCCHHHEEEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHC
RPDCVSKAFDLHWELFLGGDCGAGFKTMLSELGVNNLSWELQEFLRQAEQTQFSVTQLHQ
CHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RMREIDLFRLELALFMQDYDVLISPVFPTVAKPHGIGIKEISDFSYAMTHNLSGFPTISL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHCCHHHHHHCCCCCCCEEEE
RCGASAEGLPINILIAANRWKDTTSLAVAERLEQLMGGYKPPSLIHSLQ
EECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure
MDAIHLLSATEIIRRIKMKELSAAEVMVAHLNHIDKINPVINALTERIPPEECLKQAKEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DKSIASKKNLNKLMGLPVAIKDALYVKGLICSSACSGFYKGEKAARDATLVSRLKKEGAI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCE
ILGLTNVPELCRGGDSDNLIYGRTNNPYDLARTSGGSSGGSAALIAAGGVPFALGSDGGG
EEEECCCHHHHCCCCCCCEEECCCCCCHHHHCCCCCCCCCCEEEEEECCCCEEECCCCCC
SLMQPAHCCGIVALKPTHGHLPHTGSVGGDSYGLIGNLISFGPMARSVSDLRLGLSVLAG
CCCCCHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SDQYDPYTNPVPVMPAAPLKKLRVAYFTENGFTPVDVEIQNVVKSAALALQDDVAIVREV
CCCCCCCCCCCCCCCCCCHHHEEEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHC
RPDCVSKAFDLHWELFLGGDCGAGFKTMLSELGVNNLSWELQEFLRQAEQTQFSVTQLHQ
CHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RMREIDLFRLELALFMQDYDVLISPVFPTVAKPHGIGIKEISDFSYAMTHNLSGFPTISL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHCCHHHHHHCCCCCCCEEEE
RCGASAEGLPINILIAANRWKDTTSLAVAERLEQLMGGYKPPSLIHSLQ
EECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]