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The map label for this gene is prpD [H]

Identifier: 148359063

GI number: 148359063

Start: 1758727

End: 1760175

Strand: Reverse

Name: prpD [H]

Synonym: LPC_0950

Alternate gene names: 148359063

Gene position: 1760175-1758727 (Counterclockwise)

Preceding gene: 148359064

Following gene: 148359061

Centisome position: 49.22

GC content: 41.41

Gene sequence:

>1449_bases
ATGCACAATTATGTAGAGGATAATGTTAAGCCAGACTATGATCAGGTTATAACAGATATAGCTGATTTTGTATTAAATAA
AGAAATTGATAGTGTGCAAGCCTATGAGACTGCACGGTTGTGCTTAATGGATACACTCGGTTGTGGCATATTGGCCCTTA
ACTTTCAGGAATGTACCAAATTAATGGGACCTATAGTACCGGGAGCAACTCTTCCTGGAGGCGCGAGAGTACCAGGTACT
AATTATGAACTGGATCCGGTTCAAGCTGCCTTTAACATCGGTACGATGATACGCTGGCTAGACTTTAATGATACTTGGCT
CGCTGCAGAATGGGGGCACCCCTCTGATAATTTGGGCGCTATTCTGGCTGTTGCCGATTATTTGTGCCGGGAAAGTTGTC
ATCATGGACGTCCTCACATTTTGATGCATGATGTACTTACGGCAATGATTAAAGCACATGAAATTCAAGGTTGTTTGGCA
CTGGAAAATAGCTTCAATCGCGTCGGCCTTGATCATGTCATATTAGTCAAAGTAGCAAGTGCTGCTGTTGCTGCTCATTT
GTTTGGAGCAGATCGTGATATGATGCTAAGAACTCTCTCGCAGGTTTTTGTGGATGGACAAAGCTTGAGAACATATAGAC
ATGCTCCCAATGCAGGTTCAAGGAAATCATGGGCAGCAGGAGATGCCACCTCCCGTGCGGTTCGCTTGGCTTTAATTGCC
AAGGCGGGGGAAATGGGATACCCCAGTGCCTTGACAGCACAAACATGGGGATTTTATGACGTGCTATTTGGTAAAAAACA
CTTCAAATTTCAACGCCCTTACGGCAGCTATGTGATGGAAAATGTGTTATTCAAGTTATCGTATCCTGCTGAATTTCATG
CACAAACTGCTGTCGAATGTGCAGTAAAATTACATCCTATAGTAAAAAATAGATTAGAAGACATTTCTAGAATTGAACTA
ATCACACATGAGTCAGCAATAAGAATAATAAGTAAGCAAGGTGCTTTATATAATCCTGCCGACAGAGATCACTGCTTGCA
ATATATGGTTGCAGTAGGACTTCTCTTTGGTGATTTAAAGGCAGAGCATTATGAAGACGACGTTGCCGCAGATCCCAGAA
TAGATGCTTTACGAGCCAAAATGCATGTAACTGAAAATGAGCAGTTTTCACGCGATTATCATGATCCGGACAAACGCTCT
ATAGCTAACAGCATGCGAATCATCTTTAAAGATGGCTCAGAAAGTGAATTAATAACTATAGAATACCCTATTGGTCATAA
ACGGAGACGAGAAGAGGGGATTCCCGTTTTAATGTCTAAATTCAAGCGTAATCTGAGTACAAGATTCTCTACTGACATGG
TTGAAAAGATTATTCATACCATGAGCGACACTAACACGCTATCATCTATGAAAGTAAGTGATTTTATGGCCATGTGGATG
GTAAATTAA

Upstream 100 bases:

>100_bases
GTATTTGAGCAAAGAGCAAATAATAAACTAATAAGACCTACTTCTGAATATATAGGCCCAGATTCCAGACCATTTCCCGC
TATTGAAACACGAGGTTAAT

Downstream 100 bases:

>100_bases
AAAAACTAACACCCACGCTCAATCTGTTATCTGTACTGATTTTAGCACAGACCAGGAGGGGGCTAAGCGTATAGCAAAGC
CAACCCTCCTTACTAAATCG

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MHNYVEDNVKPDYDQVITDIADFVLNKEIDSVQAYETARLCLMDTLGCGILALNFQECTKLMGPIVPGATLPGGARVPGT
NYELDPVQAAFNIGTMIRWLDFNDTWLAAEWGHPSDNLGAILAVADYLCRESCHHGRPHILMHDVLTAMIKAHEIQGCLA
LENSFNRVGLDHVILVKVASAAVAAHLFGADRDMMLRTLSQVFVDGQSLRTYRHAPNAGSRKSWAAGDATSRAVRLALIA
KAGEMGYPSALTAQTWGFYDVLFGKKHFKFQRPYGSYVMENVLFKLSYPAEFHAQTAVECAVKLHPIVKNRLEDISRIEL
ITHESAIRIISKQGALYNPADRDHCLQYMVAVGLLFGDLKAEHYEDDVAADPRIDALRAKMHVTENEQFSRDYHDPDKRS
IANSMRIIFKDGSESELITIEYPIGHKRRREEGIPVLMSKFKRNLSTRFSTDMVEKIIHTMSDTNTLSSMKVSDFMAMWM
VN

Sequences:

>Translated_482_residues
MHNYVEDNVKPDYDQVITDIADFVLNKEIDSVQAYETARLCLMDTLGCGILALNFQECTKLMGPIVPGATLPGGARVPGT
NYELDPVQAAFNIGTMIRWLDFNDTWLAAEWGHPSDNLGAILAVADYLCRESCHHGRPHILMHDVLTAMIKAHEIQGCLA
LENSFNRVGLDHVILVKVASAAVAAHLFGADRDMMLRTLSQVFVDGQSLRTYRHAPNAGSRKSWAAGDATSRAVRLALIA
KAGEMGYPSALTAQTWGFYDVLFGKKHFKFQRPYGSYVMENVLFKLSYPAEFHAQTAVECAVKLHPIVKNRLEDISRIEL
ITHESAIRIISKQGALYNPADRDHCLQYMVAVGLLFGDLKAEHYEDDVAADPRIDALRAKMHVTENEQFSRDYHDPDKRS
IANSMRIIFKDGSESELITIEYPIGHKRRREEGIPVLMSKFKRNLSTRFSTDMVEKIIHTMSDTNTLSSMKVSDFMAMWM
VN
>Mature_482_residues
MHNYVEDNVKPDYDQVITDIADFVLNKEIDSVQAYETARLCLMDTLGCGILALNFQECTKLMGPIVPGATLPGGARVPGT
NYELDPVQAAFNIGTMIRWLDFNDTWLAAEWGHPSDNLGAILAVADYLCRESCHHGRPHILMHDVLTAMIKAHEIQGCLA
LENSFNRVGLDHVILVKVASAAVAAHLFGADRDMMLRTLSQVFVDGQSLRTYRHAPNAGSRKSWAAGDATSRAVRLALIA
KAGEMGYPSALTAQTWGFYDVLFGKKHFKFQRPYGSYVMENVLFKLSYPAEFHAQTAVECAVKLHPIVKNRLEDISRIEL
ITHESAIRIISKQGALYNPADRDHCLQYMVAVGLLFGDLKAEHYEDDVAADPRIDALRAKMHVTENEQFSRDYHDPDKRS
IANSMRIIFKDGSESELITIEYPIGHKRRREEGIPVLMSKFKRNLSTRFSTDMVEKIIHTMSDTNTLSSMKVSDFMAMWM
VN

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=478, Percent_Identity=61.7154811715481, Blast_Score=634, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325258, Length=486, Percent_Identity=56.5843621399177, Blast_Score=561, Evalue=1e-161,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 54114; Mature: 54114

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNYVEDNVKPDYDQVITDIADFVLNKEIDSVQAYETARLCLMDTLGCGILALNFQECTK
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHH
LMGPIVPGATLPGGARVPGTNYELDPVQAAFNIGTMIRWLDFNDTWLAAEWGHPSDNLGA
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCEEEEECCCCCCCHHH
ILAVADYLCRESCHHGRPHILMHDVLTAMIKAHEIQGCLALENSFNRVGLDHVILVKVAS
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
AAVAAHLFGADRDMMLRTLSQVFVDGQSLRTYRHAPNAGSRKSWAAGDATSRAVRLALIA
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEE
KAGEMGYPSALTAQTWGFYDVLFGKKHFKFQRPYGSYVMENVLFKLSYPAEFHAQTAVEC
CCCCCCCCCCCCCCCCCHHHHHHCCHHCEEECCHHHHHHHHHHHEECCCHHHHHHHHHHH
AVKLHPIVKNRLEDISRIELITHESAIRIISKQGALYNPADRDHCLQYMVAVGLLFGDLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AEHYEDDVAADPRIDALRAKMHVTENEQFSRDYHDPDKRSIANSMRIIFKDGSESELITI
HHHCCCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHCEEEEEECCCCCCEEEE
EYPIGHKRRREEGIPVLMSKFKRNLSTRFSTDMVEKIIHTMSDTNTLSSMKVSDFMAMWM
ECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
VN
CC
>Mature Secondary Structure
MHNYVEDNVKPDYDQVITDIADFVLNKEIDSVQAYETARLCLMDTLGCGILALNFQECTK
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHH
LMGPIVPGATLPGGARVPGTNYELDPVQAAFNIGTMIRWLDFNDTWLAAEWGHPSDNLGA
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCEEEEECCCCCCCHHH
ILAVADYLCRESCHHGRPHILMHDVLTAMIKAHEIQGCLALENSFNRVGLDHVILVKVAS
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
AAVAAHLFGADRDMMLRTLSQVFVDGQSLRTYRHAPNAGSRKSWAAGDATSRAVRLALIA
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEE
KAGEMGYPSALTAQTWGFYDVLFGKKHFKFQRPYGSYVMENVLFKLSYPAEFHAQTAVEC
CCCCCCCCCCCCCCCCCHHHHHHCCHHCEEECCHHHHHHHHHHHEECCCHHHHHHHHHHH
AVKLHPIVKNRLEDISRIELITHESAIRIISKQGALYNPADRDHCLQYMVAVGLLFGDLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AEHYEDDVAADPRIDALRAKMHVTENEQFSRDYHDPDKRSIANSMRIIFKDGSESELITI
HHHCCCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHCEEEEEECCCCCCEEEE
EYPIGHKRRREEGIPVLMSKFKRNLSTRFSTDMVEKIIHTMSDTNTLSSMKVSDFMAMWM
ECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
VN
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]