Click here to switch to the map view.

The map label for this gene is lpdA [H]

Identifier: 148359035

GI number: 148359035

Start: 1726168

End: 1727499

Strand: Reverse

Name: lpdA [H]

Synonym: LPC_0921

Alternate gene names: 148359035

Gene position: 1727499-1726168 (Counterclockwise)

Preceding gene: 148359036

Following gene: 148359034

Centisome position: 48.3

GC content: 39.94

Gene sequence:

>1332_bases
GTGGTTTTGGTCGAGCGTTTTGACTCCACTAGGTGGGGTTGTTTAAATGTTGGTTGCATTCCCTCAAAAGCATTATTGCA
TATTGCTAAAGTAGTAGATGAAGCCCATGAAATGTCGGAGCAAGGTGTAGCATTTGGTAAACCCAAATTCGACAATAAAA
AATTGGTTGCCTGGAAAAATTCTGTCGTTGCAAAGTTGACCGGGGGACTAAAAGCTCTTGCCAAACAAAGAAAAGTAGAA
GTAATTACTGGGACAGGTAAGTTTTCAGGTACCCATCAAATTCTTGTTGAAACGAAAGAAGGGACTGTAGAAATTGAATT
TGATAATGCCATTATTGCAGTAGGCTCAGAATCCATTAAATTACCATTTATACCAGAAGATAAACGAATATTTAGCTCTA
CAGGAGCATTAGAACTCGCTGATATCAAAGGTGACTTACTTGTATTAGGTGGTGGTATTATTGGTTTGGAAATGGCAACT
GTCTACTCTTCGCTGGGAGTTAATGTAACAGTTGTTGAATTTATGGACCAATTAATACCGAATGCTGACACTGACCTGGT
CAATATACTGCAAAAACGAATGACTAAAAAGGGCATTAAATTTCTATTAAAAACGAAAGTAACTGCTGTCGAAGCCAAAA
AAGATGGTATTTATGTTTCAATGGAGGGAGAACATGCAACTGATAAACCACTTTGTTTTCAGCAAGTACTTGTTGCAGTA
GGCAGAAAACCTAACGGTGGGGCAATCAACGCTGAAAAAGCAGGGATCAAAGTAGATGAACGTGGCTTTATTCCAGTTGA
TAATCAATTAAGAACGAATGTTCCACATATTTTTGCTATTGGCGATGTCAATGGACAACCAATGCTCGCACATAAAGCCA
TCCCTGAAGGGAAAGTTGCAGCAGAAGTCATTGCAGGGAAAAAACATTATTTTGAGCCAAAATGTATACCTAGTGTTGCT
TATACAGATCCAGAATTAGCTTGGGCTGGTCTAACAGAAAAAGAAGCAAAAGAGAAAGGAATAAACTATGAGAAAGCCTC
CTTTCCATGGGCTGCTAGCGGTCGTGCACTCAGCATGGGGCGAGAAGAGGGGATGACTAAATTACTATTCTGCCCAGAAA
CTAACCGTATCCTGGGAGCAGGTATTGTTGGGGTTAATGCCGGCGATTTAATTGCAGAAACTGCCTTAGCCATTGAAATG
TGTTGTGATGTTGAAGACATCGCTCTTACTATTCACCCTCACCCGACTCTATCAGAAACCATAGCTCAGTCAGCAGAAGC
ATTTGAAGGCACTATAACGGATCTGTATTTGCCCAAAAAGAAGAAAAAATAA

Upstream 100 bases:

>100_bases
TTCTTATGGCTAATAAAATTAAGACAGATGTAGTGGTATTAGGTAGTGGACCAGGCGGATACACCGCAGCTTTCAGGGCT
TGCCGACTTGGATAAAAAAG

Downstream 100 bases:

>100_bases
TAATTACAGGTAGCCAATTATACCTCATTGCATTTCCTTTGCTCACACAAGCAAAGGGATGCTTATTATATGTATATAAT
TATATATAGACCTCTTGCAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MVLVERFDSTRWGCLNVGCIPSKALLHIAKVVDEAHEMSEQGVAFGKPKFDNKKLVAWKNSVVAKLTGGLKALAKQRKVE
VITGTGKFSGTHQILVETKEGTVEIEFDNAIIAVGSESIKLPFIPEDKRIFSSTGALELADIKGDLLVLGGGIIGLEMAT
VYSSLGVNVTVVEFMDQLIPNADTDLVNILQKRMTKKGIKFLLKTKVTAVEAKKDGIYVSMEGEHATDKPLCFQQVLVAV
GRKPNGGAINAEKAGIKVDERGFIPVDNQLRTNVPHIFAIGDVNGQPMLAHKAIPEGKVAAEVIAGKKHYFEPKCIPSVA
YTDPELAWAGLTEKEAKEKGINYEKASFPWAASGRALSMGREEGMTKLLFCPETNRILGAGIVGVNAGDLIAETALAIEM
CCDVEDIALTIHPHPTLSETIAQSAEAFEGTITDLYLPKKKKK

Sequences:

>Translated_443_residues
MVLVERFDSTRWGCLNVGCIPSKALLHIAKVVDEAHEMSEQGVAFGKPKFDNKKLVAWKNSVVAKLTGGLKALAKQRKVE
VITGTGKFSGTHQILVETKEGTVEIEFDNAIIAVGSESIKLPFIPEDKRIFSSTGALELADIKGDLLVLGGGIIGLEMAT
VYSSLGVNVTVVEFMDQLIPNADTDLVNILQKRMTKKGIKFLLKTKVTAVEAKKDGIYVSMEGEHATDKPLCFQQVLVAV
GRKPNGGAINAEKAGIKVDERGFIPVDNQLRTNVPHIFAIGDVNGQPMLAHKAIPEGKVAAEVIAGKKHYFEPKCIPSVA
YTDPELAWAGLTEKEAKEKGINYEKASFPWAASGRALSMGREEGMTKLLFCPETNRILGAGIVGVNAGDLIAETALAIEM
CCDVEDIALTIHPHPTLSETIAQSAEAFEGTITDLYLPKKKKK
>Mature_443_residues
MVLVERFDSTRWGCLNVGCIPSKALLHIAKVVDEAHEMSEQGVAFGKPKFDNKKLVAWKNSVVAKLTGGLKALAKQRKVE
VITGTGKFSGTHQILVETKEGTVEIEFDNAIIAVGSESIKLPFIPEDKRIFSSTGALELADIKGDLLVLGGGIIGLEMAT
VYSSLGVNVTVVEFMDQLIPNADTDLVNILQKRMTKKGIKFLLKTKVTAVEAKKDGIYVSMEGEHATDKPLCFQQVLVAV
GRKPNGGAINAEKAGIKVDERGFIPVDNQLRTNVPHIFAIGDVNGQPMLAHKAIPEGKVAAEVIAGKKHYFEPKCIPSVA
YTDPELAWAGLTEKEAKEKGINYEKASFPWAASGRALSMGREEGMTKLLFCPETNRILGAGIVGVNAGDLIAETALAIEM
CCDVEDIALTIHPHPTLSETIAQSAEAFEGTITDLYLPKKKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=430, Percent_Identity=40.2325581395349, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI50301238, Length=423, Percent_Identity=25.2955082742317, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI33519430, Length=436, Percent_Identity=28.4403669724771, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI33519428, Length=436, Percent_Identity=28.4403669724771, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI33519426, Length=436, Percent_Identity=28.4403669724771, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI148277065, Length=436, Percent_Identity=28.4403669724771, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI148277071, Length=434, Percent_Identity=28.5714285714286, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI291045266, Length=444, Percent_Identity=27.2522522522523, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI22035672, Length=437, Percent_Identity=27.6887871853547, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI291045268, Length=433, Percent_Identity=25.635103926097, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI1786307, Length=443, Percent_Identity=62.0767494356659, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI87081717, Length=425, Percent_Identity=28.4705882352941, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI87082354, Length=402, Percent_Identity=26.1194029850746, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1789915, Length=413, Percent_Identity=26.1501210653753, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789065, Length=255, Percent_Identity=24.3137254901961, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32565766, Length=430, Percent_Identity=40.2325581395349, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI17557007, Length=432, Percent_Identity=28.0092592592593, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=436, Percent_Identity=26.605504587156, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71983429, Length=436, Percent_Identity=26.605504587156, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=446, Percent_Identity=25.5605381165919, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17559934, Length=223, Percent_Identity=26.9058295964126, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=424, Percent_Identity=42.4528301886792, Blast_Score=280, Evalue=5e-76,
Organism=Saccharomyces cerevisiae, GI6325240, Length=429, Percent_Identity=28.4382284382284, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6325166, Length=428, Percent_Identity=26.1682242990654, Blast_Score=133, Evalue=5e-32,
Organism=Drosophila melanogaster, GI21358499, Length=435, Percent_Identity=41.1494252873563, Blast_Score=301, Evalue=8e-82,
Organism=Drosophila melanogaster, GI24640549, Length=434, Percent_Identity=30.6451612903226, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24640553, Length=434, Percent_Identity=30.6451612903226, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24640551, Length=434, Percent_Identity=30.6451612903226, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI17737741, Length=445, Percent_Identity=28.314606741573, Blast_Score=118, Evalue=9e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 47804; Mature: 47804

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLVERFDSTRWGCLNVGCIPSKALLHIAKVVDEAHEMSEQGVAFGKPKFDNKKLVAWKN
CEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECC
SVVAKLTGGLKALAKQRKVEVITGTGKFSGTHQILVETKEGTVEIEFDNAIIAVGSESIK
CCHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEEECCCEEEEEECCEEEEECCCCEE
LPFIPEDKRIFSSTGALELADIKGDLLVLGGGIIGLEMATVYSSLGVNVTVVEFMDQLIP
ECCCCCCCHHHCCCCCEEEEECCCCEEEEECCEEEHHHHHHHHHCCCCEEHHHHHHHHCC
NADTDLVNILQKRMTKKGIKFLLKTKVTAVEAKKDGIYVSMEGEHATDKPLCFQQVLVAV
CCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHH
GRKPNGGAINAEKAGIKVDERGFIPVDNQLRTNVPHIFAIGDVNGQPMLAHKAIPEGKVA
CCCCCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCEEEEEECCCCCCEEEECCCCCCHHH
AEVIAGKKHYFEPKCIPSVAYTDPELAWAGLTEKEAKEKGINYEKASFPWAASGRALSMG
HHHHHCCHHCCCCCCCCCCCCCCCCCEECCCCHHHHHHCCCCCCCCCCCCCCCCCEEECC
REEGMTKLLFCPETNRILGAGIVGVNAGDLIAETALAIEMCCDVEDIALTIHPHPTLSET
HHCCCEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCHHHEEEEECCCCCHHHH
IAQSAEAFEGTITDLYLPKKKKK
HHHHHHHHCCCEEEEECCCCCCC
>Mature Secondary Structure
MVLVERFDSTRWGCLNVGCIPSKALLHIAKVVDEAHEMSEQGVAFGKPKFDNKKLVAWKN
CEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECC
SVVAKLTGGLKALAKQRKVEVITGTGKFSGTHQILVETKEGTVEIEFDNAIIAVGSESIK
CCHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEEECCCEEEEEECCEEEEECCCCEE
LPFIPEDKRIFSSTGALELADIKGDLLVLGGGIIGLEMATVYSSLGVNVTVVEFMDQLIP
ECCCCCCCHHHCCCCCEEEEECCCCEEEEECCEEEHHHHHHHHHCCCCEEHHHHHHHHCC
NADTDLVNILQKRMTKKGIKFLLKTKVTAVEAKKDGIYVSMEGEHATDKPLCFQQVLVAV
CCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHH
GRKPNGGAINAEKAGIKVDERGFIPVDNQLRTNVPHIFAIGDVNGQPMLAHKAIPEGKVA
CCCCCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCEEEEEECCCCCCEEEECCCCCCHHH
AEVIAGKKHYFEPKCIPSVAYTDPELAWAGLTEKEAKEKGINYEKASFPWAASGRALSMG
HHHHHCCHHCCCCCCCCCCCCCCCCCEECCCCHHHHHHCCCCCCCCCCCCCCCCCEEECC
REEGMTKLLFCPETNRILGAGIVGVNAGDLIAETALAIEMCCDVEDIALTIHPHPTLSET
HHCCCEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCHHHEEEEECCCCCHHHH
IAQSAEAFEGTITDLYLPKKKKK
HHHHHHHHCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]