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The map label for this gene is bioB [H]

Identifier: 148359001

GI number: 148359001

Start: 1688322

End: 1689269

Strand: Direct

Name: bioB [H]

Synonym: LPC_0886

Alternate gene names: 148359001

Gene position: 1688322-1689269 (Clockwise)

Preceding gene: 148358995

Following gene: 148359002

Centisome position: 47.21

GC content: 38.71

Gene sequence:

>948_bases
GTGTCTGAAGAAAATAAACAGTGGTCAATTTCTGATATTGAAGCAATTTATCAACAACCATTTAATGATTTACTTTATCA
AGCACATACCATTCATAGGACATATCACGATCCTAATTCTTTACAATTTGCAACTTTGTTAAGCATTAAAACGGGTGCAT
GCCCAGAAGATTGTGGCTATTGCTCTCAAAGTGGTCACTATAAAACGCATGTAGAAAAAGAAAAATTGATGTCAGTTGAA
GAGGTATTGCAATGTGCCAAAGAAGCAAAGGAGGGTGGGGCCAAACGTTTTTGTATGGGAGCTGCATGGCGTTGTCCACC
TGATAAAGCAATTCCGCAGCTAAAAGAAATGATAGAAGGAGTAAAGTCTTTAGGGCTTGAAACCTGTATGACTTTAGGCA
TGCTTACCAAAGAACAGGCCTCTCATTTAAAGGAGGCTGGATTGGATTATTATAACCATAATATTGATACCTCACCATCC
TATTACGATAAGGTGGTAACCACTCGAAAATTCAGTGATCGATTGGATACTTTAAATAACGTTCGTTCAGCGGGAATTAA
TGTGTGTTGTGGTGGTATTTTAGGATTGGGGGAAACTAGAGAAGATCGAATTGAATTTTTATTAACATTAGCCAATATGG
AAACTCCTCCGGAAAGTGTGCCAATTAATCGTTTAATTCCAGTGGAAGGAACACCTTTAGCCCAAGCTGAGAGAGTAGAA
GGGATTGAATTGGTAAGAACCATTGCTACAGCAAGAATTTTAATGCCTAAAAGTGCTATTAGACTGACAGCCGGTCGAAC
CGAAATGAGCGACGAGTTACAAGCACTTTGCTATTTTGCTGGAGCCAATTCTGTGTTTATTGGCGATAAGTTATTGACAG
AAGATAATCCCCAGCGCTTCAAAGACAAAACTCTTTTTAACAAACTGGGTTTGACTGAGATGGCATAA

Upstream 100 bases:

>100_bases
TTGCAATAAGTTGATCTGTGTTTACCATTTTAAAAATTTTGGTTGACAAGTATAGGCGCGACTTTATCATGTTCACTATT
CTGAATTAAAGGGGGTAGCC

Downstream 100 bases:

>100_bases
ATGACTCTTAATATAAAAATTAATGAGTATACAGCACAGCTGCAAGAACAAGGATTATTGCGTACAAGACAAATTTTAGA
AACGGATAAGATAGATTATA

Product: biotin synthase BioB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSEENKQWSISDIEAIYQQPFNDLLYQAHTIHRTYHDPNSLQFATLLSIKTGACPEDCGYCSQSGHYKTHVEKEKLMSVE
EVLQCAKEAKEGGAKRFCMGAAWRCPPDKAIPQLKEMIEGVKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPS
YYDKVVTTRKFSDRLDTLNNVRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPESVPINRLIPVEGTPLAQAERVE
GIELVRTIATARILMPKSAIRLTAGRTEMSDELQALCYFAGANSVFIGDKLLTEDNPQRFKDKTLFNKLGLTEMA

Sequences:

>Translated_315_residues
MSEENKQWSISDIEAIYQQPFNDLLYQAHTIHRTYHDPNSLQFATLLSIKTGACPEDCGYCSQSGHYKTHVEKEKLMSVE
EVLQCAKEAKEGGAKRFCMGAAWRCPPDKAIPQLKEMIEGVKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPS
YYDKVVTTRKFSDRLDTLNNVRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPESVPINRLIPVEGTPLAQAERVE
GIELVRTIATARILMPKSAIRLTAGRTEMSDELQALCYFAGANSVFIGDKLLTEDNPQRFKDKTLFNKLGLTEMA
>Mature_314_residues
SEENKQWSISDIEAIYQQPFNDLLYQAHTIHRTYHDPNSLQFATLLSIKTGACPEDCGYCSQSGHYKTHVEKEKLMSVEE
VLQCAKEAKEGGAKRFCMGAAWRCPPDKAIPQLKEMIEGVKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPSY
YDKVVTTRKFSDRLDTLNNVRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPESVPINRLIPVEGTPLAQAERVEG
IELVRTIATARILMPKSAIRLTAGRTEMSDELQALCYFAGANSVFIGDKLLTEDNPQRFKDKTLFNKLGLTEMA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=305, Percent_Identity=56.7213114754098, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321725, Length=322, Percent_Identity=47.2049689440994, Blast_Score=320, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 35136; Mature: 35004

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEENKQWSISDIEAIYQQPFNDLLYQAHTIHRTYHDPNSLQFATLLSIKTGACPEDCGY
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHEEEECCCCCCHHHCC
CSQSGHYKTHVEKEKLMSVEEVLQCAKEAKEGGAKRFCMGAAWRCPPDKAIPQLKEMIEG
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCHHHHHHHHHHHHH
VKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPSYYDKVVTTRKFSDRLDTLNN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPESVPINRLIPVEGTPLAQAERVE
HHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHHH
GIELVRTIATARILMPKSAIRLTAGRTEMSDELQALCYFAGANSVFIGDKLLTEDNPQRF
HHHHHHHHHHHHHHCCCHHHEEECCCCHHHHHHHHHHHHCCCCEEEECCCHHCCCCHHHH
KDKTLFNKLGLTEMA
HHHHHHHHCCCHHCC
>Mature Secondary Structure 
SEENKQWSISDIEAIYQQPFNDLLYQAHTIHRTYHDPNSLQFATLLSIKTGACPEDCGY
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHEEEECCCCCCHHHCC
CSQSGHYKTHVEKEKLMSVEEVLQCAKEAKEGGAKRFCMGAAWRCPPDKAIPQLKEMIEG
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCHHHHHHHHHHHHH
VKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPSYYDKVVTTRKFSDRLDTLNN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPESVPINRLIPVEGTPLAQAERVE
HHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHHH
GIELVRTIATARILMPKSAIRLTAGRTEMSDELQALCYFAGANSVFIGDKLLTEDNPQRF
HHHHHHHHHHHHHHCCCHHHEEECCCCHHHHHHHHHHHHCCCCEEEECCCHHCCCCHHHH
KDKTLFNKLGLTEMA
HHHHHHHHCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA