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The map label for this gene is glnA [H]

Identifier: 148358895

GI number: 148358895

Start: 1574183

End: 1575592

Strand: Direct

Name: glnA [H]

Synonym: LPC_0780

Alternate gene names: 148358895

Gene position: 1574183-1575592 (Clockwise)

Preceding gene: 148358886

Following gene: 148358896

Centisome position: 44.01

GC content: 39.72

Gene sequence:

>1410_bases
ATGACAAAAAATGTAGTGCTTGATGCAATCAAAGAACATGACGCCAAATTTGTGGATTTAAGATTTACAGATATCCGTGG
AAAAGAGCAACACATTACCATACCTGTTTCAGCAGTTGATGATGATTTTCTTGAGAATGGAAAAATGATCGATGGCTCAT
CTTTTAAAGGTTGGCAGAAAATTCATCAATCAGATTTGGCTTTAGTGCCAGATTTAACTTCAATTAAGCCGGATCCATTT
TTTCAGGACAACACTTTATTTATACGATGTAATGTAGTTGATCCACAGACTATGATGGGTTATGACAGATGCCCACGTTC
AATCGCACAACGCGCAGAAAATTATTTACAATCTACAGGGATTGCAGATGGTGTAAATTTTGGCCCTGAGCCTGAGTTTT
TTCTCTTCGACAATGTGCAATGGGAAACTACTATCAGCGGTTCATTTTATAAAATAGATTCAGACGAGGCACATTGGAAC
TCCGGTAAGGAAATGGAAGGCGGAAATATTGGCCATAGACCTCCGTTGAAAGGCGGTTATTTCCCTGTTCCTCCAGTCGA
TTCATCTCATGATATTCGTTCAGCAATTTGTATCACATTGGAATCTTTAGGAATGGTTGTGGAAGCACATCATCATGAAG
TAGCTACAGCTAACCAGTGTGAAGTAGCTACCCGTTTTAATACACTCACCAAAAAGGCAGACGAACTGCAAATTCTGAAA
TACGTGATACATAATGTTGCCCATAACTATGGGAAAACCGCTACTTTTATGCCAAAACCTTTGGTAGGAGATAATGGAAG
CGGGATGCACTGTCATCAATCACTTTATAAAGATGGGTATAACCTGTTTTCCGGTGATCAGTATTCGGGGCTTTCTGAAA
CAGCTTTATATTACATAGGTGGAATAATCAAGCATGCCAGAGCTTTAAACGCTTTTACCAATCCGTCAACCAATAGCTAT
AAGAGATTAGTTCCCGGGTTTGAAGCGCCTGTATTACTGGCTTACTCAGCAAGAAATCGCTCCGCCGCTATTCGTATCCC
ACACGTTAGCAATCCAAAGGCACGACGTATAGAAGTACGTTTCCCTGATCCAACAGCTAATCCTTATCTTGCTTTCTCAG
CAATGATGATGGCAGGCATAGATGGCATTCAGAAAAAAATCTACCCTGGGCAGCCAATGGATAAAGATCTTTATGATTTA
CCGCCTGAAGAGCTTGTTGATGTGCCCACTGTTTGCTCTTCATTAGAGATGGCTATGGACCATTTGCGGGCAGATCATGA
ATTTTTGTTACAGGGAGATGTGTTCAGCAGAGACTTTATTAATAATTATATTCAAATGAAAGAAGAGGAAATTACTAAAA
TCAGGAGCTTGACTCATCCTTATGAATTTGAGTTATATTATAGTCTCTAA

Upstream 100 bases:

>100_bases
CCATATTATTTTTGTTAAAATTAAAACTATAGAGTGTAATCTATTGGGATTAATAAGTCACTTAAGGTAAACTATACGCG
GACTATCCTGGAGACAAAAG

Downstream 100 bases:

>100_bases
GGGTCTATATTCATGAATAAACTGCCACTATTCGTCTTTTTGATGATGGTAATTTGCCTTTCCAGCGCGCAAATTTACAA
ATGGACGGATAGTCAGGGTG

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTKNVVLDAIKEHDAKFVDLRFTDIRGKEQHITIPVSAVDDDFLENGKMIDGSSFKGWQKIHQSDLALVPDLTSIKPDPF
FQDNTLFIRCNVVDPQTMMGYDRCPRSIAQRAENYLQSTGIADGVNFGPEPEFFLFDNVQWETTISGSFYKIDSDEAHWN
SGKEMEGGNIGHRPPLKGGYFPVPPVDSSHDIRSAICITLESLGMVVEAHHHEVATANQCEVATRFNTLTKKADELQILK
YVIHNVAHNYGKTATFMPKPLVGDNGSGMHCHQSLYKDGYNLFSGDQYSGLSETALYYIGGIIKHARALNAFTNPSTNSY
KRLVPGFEAPVLLAYSARNRSAAIRIPHVSNPKARRIEVRFPDPTANPYLAFSAMMMAGIDGIQKKIYPGQPMDKDLYDL
PPEELVDVPTVCSSLEMAMDHLRADHEFLLQGDVFSRDFINNYIQMKEEEITKIRSLTHPYEFELYYSL

Sequences:

>Translated_469_residues
MTKNVVLDAIKEHDAKFVDLRFTDIRGKEQHITIPVSAVDDDFLENGKMIDGSSFKGWQKIHQSDLALVPDLTSIKPDPF
FQDNTLFIRCNVVDPQTMMGYDRCPRSIAQRAENYLQSTGIADGVNFGPEPEFFLFDNVQWETTISGSFYKIDSDEAHWN
SGKEMEGGNIGHRPPLKGGYFPVPPVDSSHDIRSAICITLESLGMVVEAHHHEVATANQCEVATRFNTLTKKADELQILK
YVIHNVAHNYGKTATFMPKPLVGDNGSGMHCHQSLYKDGYNLFSGDQYSGLSETALYYIGGIIKHARALNAFTNPSTNSY
KRLVPGFEAPVLLAYSARNRSAAIRIPHVSNPKARRIEVRFPDPTANPYLAFSAMMMAGIDGIQKKIYPGQPMDKDLYDL
PPEELVDVPTVCSSLEMAMDHLRADHEFLLQGDVFSRDFINNYIQMKEEEITKIRSLTHPYEFELYYSL
>Mature_468_residues
TKNVVLDAIKEHDAKFVDLRFTDIRGKEQHITIPVSAVDDDFLENGKMIDGSSFKGWQKIHQSDLALVPDLTSIKPDPFF
QDNTLFIRCNVVDPQTMMGYDRCPRSIAQRAENYLQSTGIADGVNFGPEPEFFLFDNVQWETTISGSFYKIDSDEAHWNS
GKEMEGGNIGHRPPLKGGYFPVPPVDSSHDIRSAICITLESLGMVVEAHHHEVATANQCEVATRFNTLTKKADELQILKY
VIHNVAHNYGKTATFMPKPLVGDNGSGMHCHQSLYKDGYNLFSGDQYSGLSETALYYIGGIIKHARALNAFTNPSTNSYK
RLVPGFEAPVLLAYSARNRSAAIRIPHVSNPKARRIEVRFPDPTANPYLAFSAMMMAGIDGIQKKIYPGQPMDKDLYDLP
PEELVDVPTVCSSLEMAMDHLRADHEFLLQGDVFSRDFINNYIQMKEEEITKIRSLTHPYEFELYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=65.6716417910448, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=392, Percent_Identity=26.0204081632653, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52802; Mature: 52671

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNVVLDAIKEHDAKFVDLRFTDIRGKEQHITIPVSAVDDDFLENGKMIDGSSFKGWQK
CCCHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEEHHCCHHHHHCCCEECCCCCHHHHH
IHQSDLALVPDLTSIKPDPFFQDNTLFIRCNVVDPQTMMGYDRCPRSIAQRAENYLQSTG
HHHCCCEECCCHHCCCCCCCCCCCEEEEEEEECCCHHHHCHHHCHHHHHHHHHHHHHHCC
IADGVNFGPEPEFFLFDNVQWETTISGSFYKIDSDEAHWNSGKEMEGGNIGHRPPLKGGY
CCCCCCCCCCCCEEEECCEEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
FPVPPVDSSHDIRSAICITLESLGMVVEAHHHEVATANQCEVATRFNTLTKKADELQILK
CCCCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YVIHNVAHNYGKTATFMPKPLVGDNGSGMHCHQSLYKDGYNLFSGDQYSGLSETALYYIG
HHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
GIIKHARALNAFTNPSTNSYKRLVPGFEAPVLLAYSARNRSAAIRIPHVSNPKARRIEVR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCEEEECCCCCCCCCEEEEEE
FPDPTANPYLAFSAMMMAGIDGIQKKIYPGQPMDKDLYDLPPEELVDVPTVCSSLEMAMD
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHH
HLRADHEFLLQGDVFSRDFINNYIQMKEEEITKIRSLTHPYEFELYYSL
HHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
>Mature Secondary Structure 
TKNVVLDAIKEHDAKFVDLRFTDIRGKEQHITIPVSAVDDDFLENGKMIDGSSFKGWQK
CCHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEEHHCCHHHHHCCCEECCCCCHHHHH
IHQSDLALVPDLTSIKPDPFFQDNTLFIRCNVVDPQTMMGYDRCPRSIAQRAENYLQSTG
HHHCCCEECCCHHCCCCCCCCCCCEEEEEEEECCCHHHHCHHHCHHHHHHHHHHHHHHCC
IADGVNFGPEPEFFLFDNVQWETTISGSFYKIDSDEAHWNSGKEMEGGNIGHRPPLKGGY
CCCCCCCCCCCCEEEECCEEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
FPVPPVDSSHDIRSAICITLESLGMVVEAHHHEVATANQCEVATRFNTLTKKADELQILK
CCCCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YVIHNVAHNYGKTATFMPKPLVGDNGSGMHCHQSLYKDGYNLFSGDQYSGLSETALYYIG
HHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
GIIKHARALNAFTNPSTNSYKRLVPGFEAPVLLAYSARNRSAAIRIPHVSNPKARRIEVR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCEEEECCCCCCCCCEEEEEE
FPDPTANPYLAFSAMMMAGIDGIQKKIYPGQPMDKDLYDLPPEELVDVPTVCSSLEMAMD
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHH
HLRADHEFLLQGDVFSRDFINNYIQMKEEEITKIRSLTHPYEFELYYSL
HHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]