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The map label for this gene is cutE [H]

Identifier: 148358881

GI number: 148358881

Start: 1556393

End: 1557679

Strand: Reverse

Name: cutE [H]

Synonym: LPC_0765

Alternate gene names: 148358881

Gene position: 1557679-1556393 (Counterclockwise)

Preceding gene: 148358887

Following gene: 148358880

Centisome position: 43.55

GC content: 37.3

Gene sequence:

>1287_bases
GTGATTATCAGCATCCACGACTATGGACAACTTGATTACTTTCTTGCTGGAGTTATCACCTTATTGTTTATCATGTATCT
TTCTCTGTTTCCTGGAATAGTCGGTTATTCCTTTAAACTCCTTTCAGCCAAGTGTAATTTATTAACATGCATGCTTTTGT
TTAGCACTCTTTGGTGTATAGGTGAGCTCATCCGTTCCAAGCTTTTTACTGGATTCCCCTGGCTATTAACAGGTATAACC
CAAATCGATACACCATTGAGATATATGGCACCAGTAGTTGGTGTTTATGGGCTTGGATTAATATGTTCTTTTTTGGGTAC
CCTACTGGTTCTGATAATTAAAGACAATACGATTAAACGATATTACTACCTTAGCGTTTTTACCTTGATTCTTATTGCAC
CATCCCTGGGGAAAAATATCCATTGGACAGAAATCAAGAAAGAACCTATTTCTGTTGGTGCAATCCAGCCTAATCTCTCA
ATGAGAGACAAGTGGGACGAAACTCTCTTTTGGGATTTATTAAAATACTATGAAAACAAAACAGATAAACTGTTAGGAAA
AAATTTAATTGTTTTACCCGAATCAGCCATACCCCTACCAGCAAGTTATGTTGAAGATTACCTACAGAAACTGCATGAAA
AAGCATTAAAAGCCAAAAGCAGTTTAATTTTGGGAATCTTGCAACCTACAGATAATGATGAAACCAATTTTTACAACTCT
ATCATTAGCTTGGGGGAAGCTACAGGGGCCTATGTGAAACGCCAATTGGTACCATTTGGAGAATATATACCAAAACCCTT
TATCCCCATCAATCGTTGGTTAAATCTTCCAGAACCTAATGTTGTTCCCGGCAGATCGAATCAGCCATTAATTAACATCG
CTAATCATCCCATTGCCAGTCTAATTTGCTATGAAATTGCTTACCCAAATATCTTGAGATCACAAATGCCTGCAGCTCAA
TGGATAGTCTCTATCAGTGATAATGGCTGGTTTGGCCATTCCCTGGCAAGCTACCAGCAATTACAAATGGCTCAAATGCT
CTCTTTGCTTACAGGACGATACCAAATCCTGGTGAATAATGATGGACTCTCATCAGTAATTAACCACCAAGGAACTATTG
TCGAAGGGCTACCAGCTTTTAATTCAGGAATGCTCCAAACAGATATTTTTCCTGCAAATGGCATCACTCCCTGGATAATT
TGGAGTGACTATCCTGCCTTTATTTTTTGTTTCATATTTGTTCTTTTTGTTTTGTTTTTTAATATTAAACGATTTACAGA
AAAATAA

Upstream 100 bases:

>100_bases
GGCCATTTTATTTTCTAAATTTCTTCAATGCACAGTTAAGCAGGGATTTACTCTTGGTTTCCTATACGGGTTAGGATACT
TTGGCTTCGGAGTATCATGG

Downstream 100 bases:

>100_bases
TCTATTGCTGGCAAAGGCAAGAGGGGTTATCCTTAGCAGTTCCGTGTTATTAAATTTATTGGCCTATGGATAATACCTAT
AACCCCCAAGAAGTTGAAGA

Product: apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: ALP N-acyltransferase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MIISIHDYGQLDYFLAGVITLLFIMYLSLFPGIVGYSFKLLSAKCNLLTCMLLFSTLWCIGELIRSKLFTGFPWLLTGIT
QIDTPLRYMAPVVGVYGLGLICSFLGTLLVLIIKDNTIKRYYYLSVFTLILIAPSLGKNIHWTEIKKEPISVGAIQPNLS
MRDKWDETLFWDLLKYYENKTDKLLGKNLIVLPESAIPLPASYVEDYLQKLHEKALKAKSSLILGILQPTDNDETNFYNS
IISLGEATGAYVKRQLVPFGEYIPKPFIPINRWLNLPEPNVVPGRSNQPLINIANHPIASLICYEIAYPNILRSQMPAAQ
WIVSISDNGWFGHSLASYQQLQMAQMLSLLTGRYQILVNNDGLSSVINHQGTIVEGLPAFNSGMLQTDIFPANGITPWII
WSDYPAFIFCFIFVLFVLFFNIKRFTEK

Sequences:

>Translated_428_residues
MIISIHDYGQLDYFLAGVITLLFIMYLSLFPGIVGYSFKLLSAKCNLLTCMLLFSTLWCIGELIRSKLFTGFPWLLTGIT
QIDTPLRYMAPVVGVYGLGLICSFLGTLLVLIIKDNTIKRYYYLSVFTLILIAPSLGKNIHWTEIKKEPISVGAIQPNLS
MRDKWDETLFWDLLKYYENKTDKLLGKNLIVLPESAIPLPASYVEDYLQKLHEKALKAKSSLILGILQPTDNDETNFYNS
IISLGEATGAYVKRQLVPFGEYIPKPFIPINRWLNLPEPNVVPGRSNQPLINIANHPIASLICYEIAYPNILRSQMPAAQ
WIVSISDNGWFGHSLASYQQLQMAQMLSLLTGRYQILVNNDGLSSVINHQGTIVEGLPAFNSGMLQTDIFPANGITPWII
WSDYPAFIFCFIFVLFVLFFNIKRFTEK
>Mature_428_residues
MIISIHDYGQLDYFLAGVITLLFIMYLSLFPGIVGYSFKLLSAKCNLLTCMLLFSTLWCIGELIRSKLFTGFPWLLTGIT
QIDTPLRYMAPVVGVYGLGLICSFLGTLLVLIIKDNTIKRYYYLSVFTLILIAPSLGKNIHWTEIKKEPISVGAIQPNLS
MRDKWDETLFWDLLKYYENKTDKLLGKNLIVLPESAIPLPASYVEDYLQKLHEKALKAKSSLILGILQPTDNDETNFYNS
IISLGEATGAYVKRQLVPFGEYIPKPFIPINRWLNLPEPNVVPGRSNQPLINIANHPIASLICYEIAYPNILRSQMPAAQ
WIVSISDNGWFGHSLASYQQLQMAQMLSLLTGRYQILVNNDGLSSVINHQGTIVEGLPAFNSGMLQTDIFPANGITPWII
WSDYPAFIFCFIFVLFVLFFNIKRFTEK

Specific function: Transfers the fatty acyl group on membrane lipoproteins [H]

COG id: COG0815

COG function: function code M; Apolipoprotein N-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1786878, Length=437, Percent_Identity=30.6636155606407, Blast_Score=198, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004563
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 48464; Mature: 48464

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIISIHDYGQLDYFLAGVITLLFIMYLSLFPGIVGYSFKLLSAKCNLLTCMLLFSTLWCI
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GELIRSKLFTGFPWLLTGITQIDTPLRYMAPVVGVYGLGLICSFLGTLLVLIIKDNTIKR
HHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHCCCCCHHH
YYYLSVFTLILIAPSLGKNIHWTEIKKEPISVGAIQPNLSMRDKWDETLFWDLLKYYENK
HHHHHHHHHHHHHHHCCCCCEEHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCC
TDKLLGKNLIVLPESAIPLPASYVEDYLQKLHEKALKAKSSLILGILQPTDNDETNFYNS
HHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHH
IISLGEATGAYVKRQLVPFGEYIPKPFIPINRWLNLPEPNVVPGRSNQPLINIANHPIAS
HHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEECCCHHHH
LICYEIAYPNILRSQMPAAQWIVSISDNGWFGHSLASYQQLQMAQMLSLLTGRYQILVNN
HHHHHHHCHHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECC
DGLSSVINHQGTIVEGLPAFNSGMLQTDIFPANGITPWIIWSDYPAFIFCFIFVLFVLFF
CCHHHHHCCCCCEEECCCCCCCCCEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
NIKRFTEK
HHHHHHCC
>Mature Secondary Structure
MIISIHDYGQLDYFLAGVITLLFIMYLSLFPGIVGYSFKLLSAKCNLLTCMLLFSTLWCI
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GELIRSKLFTGFPWLLTGITQIDTPLRYMAPVVGVYGLGLICSFLGTLLVLIIKDNTIKR
HHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHCCCCCHHH
YYYLSVFTLILIAPSLGKNIHWTEIKKEPISVGAIQPNLSMRDKWDETLFWDLLKYYENK
HHHHHHHHHHHHHHHCCCCCEEHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCC
TDKLLGKNLIVLPESAIPLPASYVEDYLQKLHEKALKAKSSLILGILQPTDNDETNFYNS
HHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHH
IISLGEATGAYVKRQLVPFGEYIPKPFIPINRWLNLPEPNVVPGRSNQPLINIANHPIAS
HHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEECCCHHHH
LICYEIAYPNILRSQMPAAQWIVSISDNGWFGHSLASYQQLQMAQMLSLLTGRYQILVNN
HHHHHHHCHHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECC
DGLSSVINHQGTIVEGLPAFNSGMLQTDIFPANGITPWIIWSDYPAFIFCFIFVLFVLFF
CCHHHHHCCCCCEEECCCCCCCCCEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
NIKRFTEK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]