Click here to switch to the map view.

The map label for this gene is folC [H]

Identifier: 148358874

GI number: 148358874

Start: 1549050

End: 1550336

Strand: Direct

Name: folC [H]

Synonym: LPC_0758

Alternate gene names: 148358874

Gene position: 1549050-1550336 (Clockwise)

Preceding gene: 148358873

Following gene: 148358875

Centisome position: 43.31

GC content: 38.15

Gene sequence:

>1287_bases
TTGCACACGCCATGGAGTGAATGGGATTTAAATCACTGGTTGACTCATTTGGAAACCAGAAATACCCAGGAAATACAGCT
TGGTTTGACCAGAGTTTTGACTGTAGCGCAGAAATTAAATTTGCAGCGACCAAATTGCAAGGTAATAACAGTTGCAGGAA
CAAACGGTAAAGGTTCTACAGTCACAGCACTTGAAACCATCTATCACCAGTCAGGTTTTAGAGTTGGTACCTATACGTCG
CCCCATCTTCTTGTGTTTAATGAACGTATTCGAATTAATTTAACACCGATTCAAGATAAAGATTTATGTAAGGCTTTTTC
TGTGATTGAAGAAGCACGTTACCCTACCAATTTAACCTATTTTGAAATGGCAACACTTGCTGCCTTATGGTATTTCAAAC
AGCATGATCTGGATCTGATAATTCTTGAAGTAGGCTTGGGAGGACGGTTGGATGCTACAAACATTGTTGATACTGATTTA
GCGATCATTACTACTATCGACTTTGATCATCAGAATTTTCTTGGTGACACACTTGAGTTGATAGGGTATGAGAAAGCCGG
AATACTACGAAAAAATAAACCTTTTATCTACGCGGATCTCTCACCACCAAAAAGTGTCGTTGAGGTTGCATTACAACTTG
GAGCATCATCTTATTTATACGGTAAAGACTATTTTTATCAGGAGGAAGCTGACCATTGGGAGGTTACTTGTCAAGGTAAG
CCATTTGAGCAATTGACCCGGCCAAAAATTCAACTTCGTTCTGCGGCAGCAGCTGTAATGGCTTGTCATTTGATGCAAAA
TGATTTGCCTGTATCCTATAAGAATCTAAAGAAAGCGATGGAGCTCATTTATATCCCGGGGCGGTTGCAATTACAAAAAG
GAAAGACTGATATTTTGTACGATGTATCTCATAATCCGCAGTCAGTTCGATTACTTGCTGATACAATAAAAAAAATCAAT
GTAAAAGGGCAAATTCATGCTGTTTTCTCCGCTTTAAAAGATAAAGATCTTTATGGATTGATTATGCCTTTAAGGGATTG
TGTTAATCGATGGTATCCTGCACAATTACACAATAAGCGCGCTGCAAGTCAAGAGGAATTGCTTTCTGTATTCAGGGAAG
CAGAAATTTTTCTAGATGTTTGTTATAATGACCCATTTACAGCTTTTGAAATGGCATTAAAGCAAGCATCAGATGACGAT
TTAATTATTGTTTATGGTTCATTTTTTACGGTTAGTCATGTAATGGCTGCCCAACATAATCTTTTGGAGCAGAAGGAGAA
TCAATGA

Upstream 100 bases:

>100_bases
GAACATGGGCATATTGATATGATAGTTGAACGTAAACATCTTCGAGCAACTATATCCGAATTGGTTTCCAAATTGACTCA
TAAAGGAGCGGAGAGTTTTT

Downstream 100 bases:

>100_bases
AACTGATAATGGATGAGAAAGTTAAACATCGTTTGATAGGTTTGGCCGTTATTATTTCATTGGGGGCAATTTTTGCTCCT
GCACTAATAAAAAAATCCAG

Product: bifunctional protein FolC

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MHTPWSEWDLNHWLTHLETRNTQEIQLGLTRVLTVAQKLNLQRPNCKVITVAGTNGKGSTVTALETIYHQSGFRVGTYTS
PHLLVFNERIRINLTPIQDKDLCKAFSVIEEARYPTNLTYFEMATLAALWYFKQHDLDLIILEVGLGGRLDATNIVDTDL
AIITTIDFDHQNFLGDTLELIGYEKAGILRKNKPFIYADLSPPKSVVEVALQLGASSYLYGKDYFYQEEADHWEVTCQGK
PFEQLTRPKIQLRSAAAAVMACHLMQNDLPVSYKNLKKAMELIYIPGRLQLQKGKTDILYDVSHNPQSVRLLADTIKKIN
VKGQIHAVFSALKDKDLYGLIMPLRDCVNRWYPAQLHNKRAASQEELLSVFREAEIFLDVCYNDPFTAFEMALKQASDDD
LIIVYGSFFTVSHVMAAQHNLLEQKENQ

Sequences:

>Translated_428_residues
MHTPWSEWDLNHWLTHLETRNTQEIQLGLTRVLTVAQKLNLQRPNCKVITVAGTNGKGSTVTALETIYHQSGFRVGTYTS
PHLLVFNERIRINLTPIQDKDLCKAFSVIEEARYPTNLTYFEMATLAALWYFKQHDLDLIILEVGLGGRLDATNIVDTDL
AIITTIDFDHQNFLGDTLELIGYEKAGILRKNKPFIYADLSPPKSVVEVALQLGASSYLYGKDYFYQEEADHWEVTCQGK
PFEQLTRPKIQLRSAAAAVMACHLMQNDLPVSYKNLKKAMELIYIPGRLQLQKGKTDILYDVSHNPQSVRLLADTIKKIN
VKGQIHAVFSALKDKDLYGLIMPLRDCVNRWYPAQLHNKRAASQEELLSVFREAEIFLDVCYNDPFTAFEMALKQASDDD
LIIVYGSFFTVSHVMAAQHNLLEQKENQ
>Mature_428_residues
MHTPWSEWDLNHWLTHLETRNTQEIQLGLTRVLTVAQKLNLQRPNCKVITVAGTNGKGSTVTALETIYHQSGFRVGTYTS
PHLLVFNERIRINLTPIQDKDLCKAFSVIEEARYPTNLTYFEMATLAALWYFKQHDLDLIILEVGLGGRLDATNIVDTDL
AIITTIDFDHQNFLGDTLELIGYEKAGILRKNKPFIYADLSPPKSVVEVALQLGASSYLYGKDYFYQEEADHWEVTCQGK
PFEQLTRPKIQLRSAAAAVMACHLMQNDLPVSYKNLKKAMELIYIPGRLQLQKGKTDILYDVSHNPQSVRLLADTIKKIN
VKGQIHAVFSALKDKDLYGLIMPLRDCVNRWYPAQLHNKRAASQEELLSVFREAEIFLDVCYNDPFTAFEMALKQASDDD
LIIVYGSFFTVSHVMAAQHNLLEQKENQ

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=155, Percent_Identity=38.0645161290323, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI66932984, Length=155, Percent_Identity=38.0645161290323, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1788654, Length=413, Percent_Identity=39.225181598063, Blast_Score=289, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17553148, Length=346, Percent_Identity=26.878612716763, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71984923, Length=346, Percent_Identity=26.878612716763, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17553150, Length=346, Percent_Identity=26.878612716763, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323760, Length=192, Percent_Identity=35.9375, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324815, Length=238, Percent_Identity=30.672268907563, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24641571, Length=431, Percent_Identity=26.6821345707657, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24581568, Length=174, Percent_Identity=35.0574712643678, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 48756; Mature: 48756

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTPWSEWDLNHWLTHLETRNTQEIQLGLTRVLTVAQKLNLQRPNCKVITVAGTNGKGST
CCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
VTALETIYHQSGFRVGTYTSPHLLVFNERIRINLTPIQDKDLCKAFSVIEEARYPTNLTY
HHHHHHHHHHCCCEEECCCCCEEEEEECEEEEEEECCCCHHHHHHHHHHHHCCCCCCCHH
FEMATLAALWYFKQHDLDLIILEVGLGGRLDATNIVDTDLAIITTIDFDHQNFLGDTLEL
HHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCEEEEEEEECCCCHHHHHHHHH
IGYEKAGILRKNKPFIYADLSPPKSVVEVALQLGASSYLYGKDYFYQEEADHWEVTCQGK
HCCCHHCCEECCCCEEEEECCCCHHHHHHHHHHCCCCEEECCCCEECCCCCCEEEEECCC
PFEQLTRPKIQLRSAAAAVMACHLMQNDLPVSYKNLKKAMELIYIPGRLQLQKGKTDILY
CHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEECCCEEEEECCCCEEEE
DVSHNPQSVRLLADTIKKINVKGQIHAVFSALKDKDLYGLIMPLRDCVNRWYPAQLHNKR
ECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCCC
AASQEELLSVFREAEIFLDVCYNDPFTAFEMALKQASDDDLIIVYGSFFTVSHVMAAQHN
CCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHH
LLEQKENQ
HHHHCCCC
>Mature Secondary Structure
MHTPWSEWDLNHWLTHLETRNTQEIQLGLTRVLTVAQKLNLQRPNCKVITVAGTNGKGST
CCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
VTALETIYHQSGFRVGTYTSPHLLVFNERIRINLTPIQDKDLCKAFSVIEEARYPTNLTY
HHHHHHHHHHCCCEEECCCCCEEEEEECEEEEEEECCCCHHHHHHHHHHHHCCCCCCCHH
FEMATLAALWYFKQHDLDLIILEVGLGGRLDATNIVDTDLAIITTIDFDHQNFLGDTLEL
HHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCEEEEEEEECCCCHHHHHHHHH
IGYEKAGILRKNKPFIYADLSPPKSVVEVALQLGASSYLYGKDYFYQEEADHWEVTCQGK
HCCCHHCCEECCCCEEEEECCCCHHHHHHHHHHCCCCEEECCCCEECCCCCCEEEEECCC
PFEQLTRPKIQLRSAAAAVMACHLMQNDLPVSYKNLKKAMELIYIPGRLQLQKGKTDILY
CHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEECCCEEEEECCCCEEEE
DVSHNPQSVRLLADTIKKINVKGQIHAVFSALKDKDLYGLIMPLRDCVNRWYPAQLHNKR
ECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCCC
AASQEELLSVFREAEIFLDVCYNDPFTAFEMALKQASDDDLIIVYGSFFTVSHVMAAQHN
CCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHH
LLEQKENQ
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]