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The map label for this gene is hisF [H]

Identifier: 148358780

GI number: 148358780

Start: 1444975

End: 1445742

Strand: Reverse

Name: hisF [H]

Synonym: LPC_0661

Alternate gene names: 148358780

Gene position: 1445742-1444975 (Counterclockwise)

Preceding gene: 148358781

Following gene: 148358779

Centisome position: 40.42

GC content: 40.1

Gene sequence:

>768_bases
ATGTTAACCAAGCGAATCATTCCCTGTCTTGACGTTCGGGATAATCAGGTTGTCAAGGGAGTAAAATTTCGCAACCATAG
AATAATTGGAGACATACTCCCATTAGCTGCTCACTACTCAGGATCAGGCGCAGACGAATTGGTATTTTATGATATTACAG
CAAGTAGCGATGACCGATCTGTTTGTCCTGTTTGGGTCAATAAAGTGGCGGCTGCGATCAACATTCCATTTACAGTGGCT
GGAGGCATCCGAACCTTGTACCAAGCAAGATCAATATTAAATGCCGGAGCAGATAAAATCTCAATCAACAGCCCGGCGTT
GGAAAACCCTTCATTGATTAACGAATTATGCAAAGAATTTGGAAAACAATGTGTGGTAATAGGGGTTGATAGTCAGTGGA
TAGTTGATGATTATTATGTCTATCAGTATACAGGCAATGAAAATAAAAGTATCAATTCAAAACGTAAAACTCGAGAATGG
ATACGTGAAATTCAAGACCGCGGTGCTGGCGAGATAGTTCTTAATTGCATGCAATCAGATGGCGTTCGAATGGGATATGA
TATTGCACAGCTATCAGAAATGATAAACCTCTGCCAAGTTCCTCTCATCGCATCGGGCGGTGCTGGAAAAATTAATGATT
TTGTTGAAGTATTTAAAAAGTCTCATGTCAGCGGGGCTTTGGCAGCAAGCGTTTTTCATGACAAAATACTGGCAATCAAT
GACATAAAACTAGCATTACAAACAGAAAATATTGAGGTAAGACTATGA

Upstream 100 bases:

>100_bases
GCCATATGCAGGATATTACAACGCTGGGTTCATTAGGAATATCCGCCGTAATCCTTGGGCTAATGCTTTATCAAGATAAT
GTGAGTTTCGAGGAGTTATT

Downstream 100 bases:

>100_bases
GTGACTTGGACATTAACTCTCTTGATTGGCAAAAAATGAATGGACTATTGCCGGCAATAATCCAAAACGCAGAAAATGGT
AAAGTATTGATGCTAGGTTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGDILPLAAHYSGSGADELVFYDITASSDDRSVCPVWVNKVAAAINIPFTVA
GGIRTLYQARSILNAGADKISINSPALENPSLINELCKEFGKQCVVIGVDSQWIVDDYYVYQYTGNENKSINSKRKTREW
IREIQDRGAGEIVLNCMQSDGVRMGYDIAQLSEMINLCQVPLIASGGAGKINDFVEVFKKSHVSGALAASVFHDKILAIN
DIKLALQTENIEVRL

Sequences:

>Translated_255_residues
MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGDILPLAAHYSGSGADELVFYDITASSDDRSVCPVWVNKVAAAINIPFTVA
GGIRTLYQARSILNAGADKISINSPALENPSLINELCKEFGKQCVVIGVDSQWIVDDYYVYQYTGNENKSINSKRKTREW
IREIQDRGAGEIVLNCMQSDGVRMGYDIAQLSEMINLCQVPLIASGGAGKINDFVEVFKKSHVSGALAASVFHDKILAIN
DIKLALQTENIEVRL
>Mature_255_residues
MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGDILPLAAHYSGSGADELVFYDITASSDDRSVCPVWVNKVAAAINIPFTVA
GGIRTLYQARSILNAGADKISINSPALENPSLINELCKEFGKQCVVIGVDSQWIVDDYYVYQYTGNENKSINSKRKTREW
IREIQDRGAGEIVLNCMQSDGVRMGYDIAQLSEMINLCQVPLIASGGAGKINDFVEVFKKSHVSGALAASVFHDKILAIN
DIKLALQTENIEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=58.3657587548638, Blast_Score=310, Evalue=4e-86,
Organism=Escherichia coli, GI87082028, Length=231, Percent_Identity=24.6753246753247, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319725, Length=318, Percent_Identity=30.188679245283, Blast_Score=125, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 28131; Mature: 28131

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGDILPLAAHYSGSGADELVFYDITASSDDRS
CCCCCCCCCCCCCCCCCEECEEECCCCHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCC
VCPVWVNKVAAAINIPFTVAGGIRTLYQARSILNAGADKISINSPALENPSLINELCKEF
CCHHHHHHHHHHEECCEEECHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHH
GKQCVVIGVDSQWIVDDYYVYQYTGNENKSINSKRKTREWIREIQDRGAGEIVLNCMQSD
CCEEEEEECCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHCC
GVRMGYDIAQLSEMINLCQVPLIASGGAGKINDFVEVFKKSHVSGALAASVFHDKILAIN
CCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHEEEEE
DIKLALQTENIEVRL
EEEEEEEECCEEEEC
>Mature Secondary Structure
MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGDILPLAAHYSGSGADELVFYDITASSDDRS
CCCCCCCCCCCCCCCCCEECEEECCCCHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCC
VCPVWVNKVAAAINIPFTVAGGIRTLYQARSILNAGADKISINSPALENPSLINELCKEF
CCHHHHHHHHHHEECCEEECHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHH
GKQCVVIGVDSQWIVDDYYVYQYTGNENKSINSKRKTREWIREIQDRGAGEIVLNCMQSD
CCEEEEEECCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHCC
GVRMGYDIAQLSEMINLCQVPLIASGGAGKINDFVEVFKKSHVSGALAASVFHDKILAIN
CCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHEEEEE
DIKLALQTENIEVRL
EEEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA