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The map label for this gene is ribA [H]

Identifier: 148358766

GI number: 148358766

Start: 1426820

End: 1428028

Strand: Direct

Name: ribA [H]

Synonym: LPC_0646

Alternate gene names: 148358766

Gene position: 1426820-1428028 (Clockwise)

Preceding gene: 148358765

Following gene: 148358767

Centisome position: 39.89

GC content: 40.36

Gene sequence:

>1209_bases
ATGAAACATTCATTAGCAACAATAGAAGAGGCTGTGGCGACACTTAAAGCCGGTAAAATGATTATTCTAATGGATGATGA
AGATAGAGAGAATGAAGGTGATTTGGTGATTGCTGCTGAGCACGCTACTCCTGAAGCCATTAATTTTATGTCTCGTCTTG
GTTGTGGTTTAATTTGTTTGCCTATGGCTGAAGAATTGATAGACAAACTCCAATTGCCCATGATGGCTCGGCATAACAAA
TCGCCGTATGGAACGGCGTTTACGGTTTCGATTGAAGCAGCTAACGGTGTCTCCACAGGCATCTCAGCAAGAGACAGAGC
TCGAACAATTCAAGTGGCTATTGATCCAAAAAGTGGGCCATCTGATATCATTTCACCTGGACATGTTTTTCCTTTACGGG
CTAGAAAGAGAGGAGTTTTAGAAAGACCAGGTCAAACAGAAGGTAGTGTTGATTTGGTAAGACTTGCCGGATTGACCCCG
GCGGCAGTGATTTGTGAGATCATTAATGAAGATGGAACAATGAGCAGACGTGATGAATTAGCTCTTTTTTCAAAAAAACA
TCAAATACCTTTAGTCACCATTAAAGATTTGATTAATTACCGAATTCGTCACGAAAATCTTATTAATCCTGTCGCAACCA
CTCAGATTCCTCTGGAGGAGAATGGTGTTTTTACTATGACTGTTTTTGAGAATCAGATAGATTCCGGTGAACATTTTGTC
TTGGTTAAACCCCCTTTGTATGGTAATCAAGTGCCTTTGGTTCGAATTCATTCTGAGTGCATAACAGGTGATGTTTTTGG
CTCCAGAAAATGTGATTGTGGAAAACAACTGGAGTTGTCTTTATCCCAGATCGGAGCTGAAGGCGGTATATTAATTTATT
TGCGTCAGGAAGGCCGTGGAATAGGTTTGGCAAACAAATTAAAGGCGTATGCTTTGCAAGAGCAGGGGTTTGATACAGTC
GATGCTAATCTGGAATTAGGATTGCCCGCTGACGATAGAGACTATGCCGTAGCATACCAAATACTGAAATATTTGGGGGT
TGACGCATTAAGGTTGCTGACAAATAATCCTTTAAAAATCGCTTCTCTTGAGCGTTATGGAATCAAGATTACTCAACGCA
TTCCTTTGGAAATTAAGCCCTCCAGAGAAAATCACAGTTATTTAAAAACCAAAAAAATAAAGCTGGGGCATTTATTAGCC
ATTGATTAG

Upstream 100 bases:

>100_bases
TAAGAGTGGGCCAGCAAGTCAATATTGAATTTGATTATCTTACCCGTATTGTGGCTCATCAGCTTCAAATTATCGGGAAA
TTGAACCATGAGGAAGTGCC

Downstream 100 bases:

>100_bases
GATAAACATGAGAGAGATAAAAACAATTGTTAGTGATGGTGTCACGTTTCCAATAGCGCTAGTAGTAAGTACTTTTAATG
AATTGATAACCTCTGCATTA

Product: riboflavin biosynthesis protein RibA

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKHSLATIEEAVATLKAGKMIILMDDEDRENEGDLVIAAEHATPEAINFMSRLGCGLICLPMAEELIDKLQLPMMARHNK
SPYGTAFTVSIEAANGVSTGISARDRARTIQVAIDPKSGPSDIISPGHVFPLRARKRGVLERPGQTEGSVDLVRLAGLTP
AAVICEIINEDGTMSRRDELALFSKKHQIPLVTIKDLINYRIRHENLINPVATTQIPLEENGVFTMTVFENQIDSGEHFV
LVKPPLYGNQVPLVRIHSECITGDVFGSRKCDCGKQLELSLSQIGAEGGILIYLRQEGRGIGLANKLKAYALQEQGFDTV
DANLELGLPADDRDYAVAYQILKYLGVDALRLLTNNPLKIASLERYGIKITQRIPLEIKPSRENHSYLKTKKIKLGHLLA
ID

Sequences:

>Translated_402_residues
MKHSLATIEEAVATLKAGKMIILMDDEDRENEGDLVIAAEHATPEAINFMSRLGCGLICLPMAEELIDKLQLPMMARHNK
SPYGTAFTVSIEAANGVSTGISARDRARTIQVAIDPKSGPSDIISPGHVFPLRARKRGVLERPGQTEGSVDLVRLAGLTP
AAVICEIINEDGTMSRRDELALFSKKHQIPLVTIKDLINYRIRHENLINPVATTQIPLEENGVFTMTVFENQIDSGEHFV
LVKPPLYGNQVPLVRIHSECITGDVFGSRKCDCGKQLELSLSQIGAEGGILIYLRQEGRGIGLANKLKAYALQEQGFDTV
DANLELGLPADDRDYAVAYQILKYLGVDALRLLTNNPLKIASLERYGIKITQRIPLEIKPSRENHSYLKTKKIKLGHLLA
ID
>Mature_402_residues
MKHSLATIEEAVATLKAGKMIILMDDEDRENEGDLVIAAEHATPEAINFMSRLGCGLICLPMAEELIDKLQLPMMARHNK
SPYGTAFTVSIEAANGVSTGISARDRARTIQVAIDPKSGPSDIISPGHVFPLRARKRGVLERPGQTEGSVDLVRLAGLTP
AAVICEIINEDGTMSRRDELALFSKKHQIPLVTIKDLINYRIRHENLINPVATTQIPLEENGVFTMTVFENQIDSGEHFV
LVKPPLYGNQVPLVRIHSECITGDVFGSRKCDCGKQLELSLSQIGAEGGILIYLRQEGRGIGLANKLKAYALQEQGFDTV
DANLELGLPADDRDYAVAYQILKYLGVDALRLLTNNPLKIASLERYGIKITQRIPLEIKPSRENHSYLKTKKIKLGHLLA
ID

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate [H]

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1789420, Length=198, Percent_Identity=51.5151515151515, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1787533, Length=190, Percent_Identity=50, Blast_Score=182, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320695, Length=201, Percent_Identity=43.7810945273632, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6319438, Length=170, Percent_Identity=45.2941176470588, Blast_Score=129, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12; =3.5.4.25 [H]

Molecular weight: Translated: 44245; Mature: 44245

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHSLATIEEAVATLKAGKMIILMDDEDRENEGDLVIAAEHATPEAINFMSRLGCGLICL
CCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEH
PMAEELIDKLQLPMMARHNKSPYGTAFTVSIEAANGVSTGISARDRARTIQVAIDPKSGP
HHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCC
SDIISPGHVFPLRARKRGVLERPGQTEGSVDLVRLAGLTPAAVICEIINEDGTMSRRDEL
HHCCCCCCEEEECHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHH
ALFSKKHQIPLVTIKDLINYRIRHENLINPVATTQIPLEENGVFTMTVFENQIDSGEHFV
HHHHCCCCCCEEEHHHHHHHHCCCCCCCCCCCEEECEECCCCEEEEEEEECCCCCCCEEE
LVKPPLYGNQVPLVRIHSECITGDVFGSRKCDCGKQLELSLSQIGAEGGILIYLRQEGRG
EECCCCCCCCCCEEEEECHHEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCC
IGLANKLKAYALQEQGFDTVDANLELGLPADDRDYAVAYQILKYLGVDALRLLTNNPLKI
CCHHHHHHHHHHHHCCCCEECCCEEECCCCCCCCHHHHHHHHHHHCHHHHHHHCCCCEEE
ASLERYGIKITQRIPLEIKPSRENHSYLKTKKIKLGHLLAID
EEEHCCCEEEEEECCEEECCCCCCCCEEEEEEEEECEEEEEC
>Mature Secondary Structure
MKHSLATIEEAVATLKAGKMIILMDDEDRENEGDLVIAAEHATPEAINFMSRLGCGLICL
CCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEH
PMAEELIDKLQLPMMARHNKSPYGTAFTVSIEAANGVSTGISARDRARTIQVAIDPKSGP
HHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCC
SDIISPGHVFPLRARKRGVLERPGQTEGSVDLVRLAGLTPAAVICEIINEDGTMSRRDEL
HHCCCCCCEEEECHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHH
ALFSKKHQIPLVTIKDLINYRIRHENLINPVATTQIPLEENGVFTMTVFENQIDSGEHFV
HHHHCCCCCCEEEHHHHHHHHCCCCCCCCCCCEEECEECCCCEEEEEEEECCCCCCCEEE
LVKPPLYGNQVPLVRIHSECITGDVFGSRKCDCGKQLELSLSQIGAEGGILIYLRQEGRG
EECCCCCCCCCCEEEEECHHEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCC
IGLANKLKAYALQEQGFDTVDANLELGLPADDRDYAVAYQILKYLGVDALRLLTNNPLKI
CCHHHHHHHHHHHHCCCCEECCCEEECCCCCCCCHHHHHHHHHHHCHHHHHHHCCCCEEE
ASLERYGIKITQRIPLEIKPSRENHSYLKTKKIKLGHLLAID
EEEHCCCEEEEEECCEEECCCCCCCCEEEEEEEEECEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA