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The map label for this gene is ddlA

Identifier: 148358653

GI number: 148358653

Start: 3124523

End: 3125617

Strand: Reverse

Name: ddlA

Synonym: LPC_0529

Alternate gene names: 148358653

Gene position: 3125617-3124523 (Counterclockwise)

Preceding gene: 148358652

Following gene: 148358654

Centisome position: 87.39

GC content: 39.09

Gene sequence:

>1095_bases
ATGTCCAAACCCGTTAATTTGGTTTTACTTTATGGTGGAAAATCAGGCGAGCATGAAGTGTCACTGGTTTCTGCCGCATC
GGTTCTAAAACACCTGGATTCCGAGAAATATCATATTATACCCATTGCAATGGACAAAAGCGGTCGATTTCATCGTCATG
ATTATAATGATTTGCTCGCCTGTTCTGATAAATTACCTGTTGTTACTGAAAAATCAACCCCGTTGGAAGGCTTATTAATT
AATGGACGTCTGGCAGTTGATGCTGAAATTGTTTTTCCAGTGGTTCATGGCCCTTTATATGAGGACGGATGTTTACAAGG
TTTACTTGAGTTAGCCGGTGTCGCCTATGTAGGATGTGACGTATTGTCTTCTGCAATCGGAATGGACAAAGACATGGCGA
GGCGACTCGCATGCATTAATGGGCTCAAATCAGCAAGATATAAATTATTATCATGGCATGCCAATGCGTCTGAAAGACAA
CAATTTTGTCATGAAGTTGCCTCGGAGTTTGGTTGGCCTCTGTTCGTTAAACCATGCTCACTAGGTTCCAGTGTGGGAAT
TCACAAAGCAAATAATATGGATGAGCTAAACGCAGCAGTTGCTGATGCATTACGCTATGATGAAGAAATTCTGGTTGAAG
AATTTATTGTCGGAAGGGAAATTGAGCTGGCAGTGTTGGAGAACAGTATTCCCTGTGGGAAACCAAGAGTAAGTATGGTG
GGTGAGATAAAAGTAAACCACCCTGACGGTTATTATTCTTATACTGCGAAATATTTGGAAAGCAGTCAAACGGATTTGAT
TATTCCAGCTCAATTAAATAATAGTCTGGAAGAACAATTAAAACAGGCTGCAGCGAATATTTTTAGTTATCTTAAATGCA
AAGGCATGGCTAGGGTAGATTTTTTTGTAAATGATAAGACAGAAGAAATTTACTTTAATGAAATAAATACATTACCTGGA
TTTACCTCCATAAGTATGTACCCCAAATTGTGGCAGGCAACTGGTGTAGCTTATCCTGATTTACTTGATGAATTGATTAA
TCTGGCAATGGTTCATCATAATTGTCGTCAACATTTGGTGACAAATTACTTGTGA

Upstream 100 bases:

>100_bases
GCAGCTAATATTGAAGCCTTGATTCATTTGGTGCAAACTAAAGTTCGAGAGCAAACATCTATAGAGTTGATACGCGAAGT
ACATATCATTGGAGACGCTA

Downstream 100 bases:

>100_bases
AGGGAACACTAAATTATGGAAGTGAACTCTGTCGGCTTGCGTTATTTAACGATAATGGGTTTGTTAATTCTTAGTGCTTT
GCTGCTAGCGGGTCGCTTAG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAMDKSGRFHRHDYNDLLACSDKLPVVTEKSTPLEGLLI
NGRLAVDAEIVFPVVHGPLYEDGCLQGLLELAGVAYVGCDVLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQ
QFCHEVASEFGWPLFVKPCSLGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVGREIELAVLENSIPCGKPRVSMV
GEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSLEEQLKQAAANIFSYLKCKGMARVDFFVNDKTEEIYFNEINTLPG
FTSISMYPKLWQATGVAYPDLLDELINLAMVHHNCRQHLVTNYL

Sequences:

>Translated_364_residues
MSKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAMDKSGRFHRHDYNDLLACSDKLPVVTEKSTPLEGLLI
NGRLAVDAEIVFPVVHGPLYEDGCLQGLLELAGVAYVGCDVLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQ
QFCHEVASEFGWPLFVKPCSLGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVGREIELAVLENSIPCGKPRVSMV
GEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSLEEQLKQAAANIFSYLKCKGMARVDFFVNDKTEEIYFNEINTLPG
FTSISMYPKLWQATGVAYPDLLDELINLAMVHHNCRQHLVTNYL
>Mature_363_residues
SKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAMDKSGRFHRHDYNDLLACSDKLPVVTEKSTPLEGLLIN
GRLAVDAEIVFPVVHGPLYEDGCLQGLLELAGVAYVGCDVLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQQ
FCHEVASEFGWPLFVKPCSLGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVGREIELAVLENSIPCGKPRVSMVG
EIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSLEEQLKQAAANIFSYLKCKGMARVDFFVNDKTEEIYFNEINTLPGF
TSISMYPKLWQATGVAYPDLLDELINLAMVHHNCRQHLVTNYL

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=366, Percent_Identity=38.7978142076503, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1786280, Length=354, Percent_Identity=31.3559322033898, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_LEGPA (Q5X1S8)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_124970.1
- HSSP:   P25051
- ProteinModelPortal:   Q5X1S8
- SMR:   Q5X1S8
- STRING:   Q5X1S8
- GeneID:   3119457
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp2665
- LegioList:   lpp2665
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   LPNE297246:LPP2665-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 40410; Mature: 40279

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAMDKSGRFHRHDYNDLLA
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHC
CSDKLPVVTEKSTPLEGLLINGRLAVDAEIVFPVVHGPLYEDGCLQGLLELAGVAYVGCD
CCCCCCEEECCCCCCCEEEECCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
VLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQQFCHEVASEFGWPLFVKPCS
HHHHHHCCCHHHHHHHHHHHHHHHHHEEHEEECCCHHHHHHHHHHHHHHCCCEEEEEECC
LGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVGREIELAVLENSIPCGKPRVSMV
CCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEE
GEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSLEEQLKQAAANIFSYLKCKGMARVD
EEEEEECCCCHHHHHHHHHCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEE
FFVNDKTEEIYFNEINTLPGFTSISMYPKLWQATGVAYPDLLDELINLAMVHHNCRQHLV
EEECCCCCEEEEEHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
TNYL
HHCC
>Mature Secondary Structure 
SKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAMDKSGRFHRHDYNDLLA
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHC
CSDKLPVVTEKSTPLEGLLINGRLAVDAEIVFPVVHGPLYEDGCLQGLLELAGVAYVGCD
CCCCCCEEECCCCCCCEEEECCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
VLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQQFCHEVASEFGWPLFVKPCS
HHHHHHCCCHHHHHHHHHHHHHHHHHEEHEEECCCHHHHHHHHHHHHHHCCCEEEEEECC
LGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVGREIELAVLENSIPCGKPRVSMV
CCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEE
GEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSLEEQLKQAAANIFSYLKCKGMARVD
EEEEEECCCCHHHHHHHHHCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEE
FFVNDKTEEIYFNEINTLPGFTSISMYPKLWQATGVAYPDLLDELINLAMVHHNCRQHLV
EEECCCCCEEEEEHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
TNYL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA