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The map label for this gene is murF [H]

Identifier: 148358647

GI number: 148358647

Start: 3131693

End: 3132913

Strand: Reverse

Name: murF [H]

Synonym: LPC_0523

Alternate gene names: 148358647

Gene position: 3132913-3131693 (Counterclockwise)

Preceding gene: 148358646

Following gene: 148358648

Centisome position: 87.6

GC content: 43.82

Gene sequence:

>1221_bases
GTGGGGGAACGCTTTGATGGTCATGATTTTATTAAAGAAGCCGAGGCTAAAGGCGCTTCAGCTGCTGTAGTTAATCGATT
GATTGACGGCGTTCATATTCCTCAATTTGTGGTTGTCAGTCCTTTAATGGCTTTGGCCAAAATTGCTGCGGCACATCGAC
AGGAAATGAGTTGCCCTATTATTGCTTTAACAGGGTCAAATGGAAAAACCACTGTCAAAGAGATGATAGCGACTATTTTA
CCGCAGCCATCACACGCTACGAAAGGCAATTTAAATAATCACATAGGGGTTCCTTTAAGCATGTTAGCTTTAAATAAGCA
GCACCGCTATGCTGTTTTTGAGTTGGGAGCCAATCATCCCGGAGAAATAGCGCATACGGTAGGGATAGTGCAGCCTGATG
TGTCTTTAATTAATAATATTGCCCCGGCACATGTCGAAGGCTTCGGATCAATAGACGGAGTCGCTCGTTCCAAAGGTGAA
ATCCATCAAGGCTTATCCCCGACTGGTGTTGCTATAGTCAATGATGATGATGCCTACGCTCACTTTTGGGATGATTTGAT
TGCTGATAAAAAAATATTACGTTTTTCCTCACTTCATCCTGCAGATATTTACGCCCAGGATATACGCTTGGATACCCATG
GGCGAGCTCATTTTACATTGGTTTTACCTAATAGCAGAGTGGAGATTGAGTTACAAGTACCAGGTTTGCACAATGTCCGA
AATGCTTTGGCTGCCGCAGCTTGTTGTTATGCTGTAGGAATATCAGGCAATGACATTCAAAGAGGACTGGCTCATTTTGG
TGGTGTAAAAGGTCGGATGACGATGTTGTCAGGGCAAAATCAATCAACAGTTATTGATGATACATACAATGCTAATTTAC
GCTCTGTCTTAACAGCCCTGGAAGTTTTATCTGGGCGCCCAGGAAGAAAAATTTTTGTATTCGGTGATATGGGAGAGTTG
GGTGAATGGACGACACAACATCACAGAGAAGTTGGCATAGCGGCTCGCCAGCTGGGAATAGATAAGTTGATGAGTTGCGG
TTTACATAGTGAACTGGCATCCCAGGCGTTTGGTGAGGGAGGCCAGCATTTTTGCAGTCAAGAAGAGTTGATTGAAGTGG
TAAAAAATGATTTAACACCTGATACCACGGTATTGGTAAAAGGATCCAGGTCCAGTGCTATGGAAAATGTTGTGCACCGG
TTGGTTCAGGTAGAGGGCTAA

Upstream 100 bases:

>100_bases
TGCTTCACTTCTTTCTCAGTCTTGCCAGATTAATGCCGACATTACTGGTGTTAGTATAGATACCCGCACATTAAAGCCAG
GAAATTTATTTTTTGCGATA

Downstream 100 bases:

>100_bases
CCTCTTCATTGAATCGACAGGAATGTGGTATGCTTGGCCACTTTTTTGCCCCATTAAAGGAATTATGCTCAATAAAAGTC
ATTGAGCATAATTTTAAGAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6- diaminopimelate-D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MGERFDGHDFIKEAEAKGASAAVVNRLIDGVHIPQFVVVSPLMALAKIAAAHRQEMSCPIIALTGSNGKTTVKEMIATIL
PQPSHATKGNLNNHIGVPLSMLALNKQHRYAVFELGANHPGEIAHTVGIVQPDVSLINNIAPAHVEGFGSIDGVARSKGE
IHQGLSPTGVAIVNDDDAYAHFWDDLIADKKILRFSSLHPADIYAQDIRLDTHGRAHFTLVLPNSRVEIELQVPGLHNVR
NALAAAACCYAVGISGNDIQRGLAHFGGVKGRMTMLSGQNQSTVIDDTYNANLRSVLTALEVLSGRPGRKIFVFGDMGEL
GEWTTQHHREVGIAARQLGIDKLMSCGLHSELASQAFGEGGQHFCSQEELIEVVKNDLTPDTTVLVKGSRSSAMENVVHR
LVQVEG

Sequences:

>Translated_406_residues
MGERFDGHDFIKEAEAKGASAAVVNRLIDGVHIPQFVVVSPLMALAKIAAAHRQEMSCPIIALTGSNGKTTVKEMIATIL
PQPSHATKGNLNNHIGVPLSMLALNKQHRYAVFELGANHPGEIAHTVGIVQPDVSLINNIAPAHVEGFGSIDGVARSKGE
IHQGLSPTGVAIVNDDDAYAHFWDDLIADKKILRFSSLHPADIYAQDIRLDTHGRAHFTLVLPNSRVEIELQVPGLHNVR
NALAAAACCYAVGISGNDIQRGLAHFGGVKGRMTMLSGQNQSTVIDDTYNANLRSVLTALEVLSGRPGRKIFVFGDMGEL
GEWTTQHHREVGIAARQLGIDKLMSCGLHSELASQAFGEGGQHFCSQEELIEVVKNDLTPDTTVLVKGSRSSAMENVVHR
LVQVEG
>Mature_405_residues
GERFDGHDFIKEAEAKGASAAVVNRLIDGVHIPQFVVVSPLMALAKIAAAHRQEMSCPIIALTGSNGKTTVKEMIATILP
QPSHATKGNLNNHIGVPLSMLALNKQHRYAVFELGANHPGEIAHTVGIVQPDVSLINNIAPAHVEGFGSIDGVARSKGEI
HQGLSPTGVAIVNDDDAYAHFWDDLIADKKILRFSSLHPADIYAQDIRLDTHGRAHFTLVLPNSRVEIELQVPGLHNVRN
ALAAAACCYAVGISGNDIQRGLAHFGGVKGRMTMLSGQNQSTVIDDTYNANLRSVLTALEVLSGRPGRKIFVFGDMGELG
EWTTQHHREVGIAARQLGIDKLMSCGLHSELASQAFGEGGQHFCSQEELIEVVKNDLTPDTTVLVKGSRSSAMENVVHRL
VQVEG

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=405, Percent_Identity=45.1851851851852, Blast_Score=301, Evalue=7e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 43626; Mature: 43495

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGERFDGHDFIKEAEAKGASAAVVNRLIDGVHIPQFVVVSPLMALAKIAAAHRQEMSCPI
CCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
IALTGSNGKTTVKEMIATILPQPSHATKGNLNNHIGVPLSMLALNKQHRYAVFELGANHP
EEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCC
GEIAHTVGIVQPDVSLINNIAPAHVEGFGSIDGVARSKGEIHQGLSPTGVAIVNDDDAYA
CCHHHHHCCCCCCHHHHHCCCCHHHCCCCCCCHHHHCCHHHHCCCCCCCEEEECCCCHHH
HFWDDLIADKKILRFSSLHPADIYAQDIRLDTHGRAHFTLVLPNSRVEIELQVPGLHNVR
HHHHHHHHCHHHHHHCCCCCHHHHHHHEEEECCCCEEEEEEECCCEEEEEEECCCHHHHH
NALAAAACCYAVGISGNDIQRGLAHFGGVKGRMTMLSGQNQSTVIDDTYNANLRSVLTAL
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCHHHHHHHH
EVLSGRPGRKIFVFGDMGELGEWTTQHHREVGIAARQLGIDKLMSCGLHSELASQAFGEG
HHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHCCC
GQHFCSQEELIEVVKNDLTPDTTVLVKGSRSSAMENVVHRLVQVEG
HHHHCCHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GERFDGHDFIKEAEAKGASAAVVNRLIDGVHIPQFVVVSPLMALAKIAAAHRQEMSCPI
CCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
IALTGSNGKTTVKEMIATILPQPSHATKGNLNNHIGVPLSMLALNKQHRYAVFELGANHP
EEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCC
GEIAHTVGIVQPDVSLINNIAPAHVEGFGSIDGVARSKGEIHQGLSPTGVAIVNDDDAYA
CCHHHHHCCCCCCHHHHHCCCCHHHCCCCCCCHHHHCCHHHHCCCCCCCEEEECCCCHHH
HFWDDLIADKKILRFSSLHPADIYAQDIRLDTHGRAHFTLVLPNSRVEIELQVPGLHNVR
HHHHHHHHCHHHHHHCCCCCHHHHHHHEEEECCCCEEEEEEECCCEEEEEEECCCHHHHH
NALAAAACCYAVGISGNDIQRGLAHFGGVKGRMTMLSGQNQSTVIDDTYNANLRSVLTAL
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCHHHHHHHH
EVLSGRPGRKIFVFGDMGELGEWTTQHHREVGIAARQLGIDKLMSCGLHSELASQAFGEG
HHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHCCC
GQHFCSQEELIEVVKNDLTPDTTVLVKGSRSSAMENVVHRLVQVEG
HHHHCCHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]