Click here to switch to the map view.

The map label for this gene is pheS [H]

Identifier: 148358552

GI number: 148358552

Start: 3239892

End: 3240908

Strand: Reverse

Name: pheS [H]

Synonym: LPC_0424

Alternate gene names: 148358552

Gene position: 3240908-3239892 (Counterclockwise)

Preceding gene: 148358551

Following gene: 148358553

Centisome position: 90.62

GC content: 40.9

Gene sequence:

>1017_bases
ATGTTGGTACTTATAAACACCATACAAGAACAAGCTTCAGAAGCAATCAAACAAGCCACGGATATAGTTGCTCTTGAACA
AATTCGTGTTGATTTTTTGGGCAAGAAAGGCAAGCTCACCGAATTGCTAAAAGGACTTGCTAATTTGTCTGCAGAGGAAA
AACCCAAGGTAGGTCAATTGGTCAATCAAGCAAAGCAGGGAATTAGTGCTTTGATTGAAACAAAAATGATCGAGCTGAAA
GAAAAGCAATTATTAGCCAAGCTGGCAGCAGAGCAAATCGACGTTACTCTTCCAGGCCGCAATCATTCCACAGGTTCCCT
ACATCCTGTTACTCAGGTAAAGCATAGAATTAATGATTATTTTAGTCGTTTAGGATTTGATATTGTTGAAGGTCCTGAAG
TTGAAACAGAATTTTATAATTTTGAAGCATTAAATATCCCTGGCCATCATCCTGCACGTGCGATGCACGATACGTTTTAT
TTTGGAGACGGACGCTTGCTGAGGACACACACATCTCCAGTACAAATCAGGACAATGGAGCAACGTAAGCCACCTTTTCG
TTTAATTGCTCCTGGCCGAGTTTATCGTTGTGATTCGGATGTAACCCATACCCCTATGTTTCATCAAGTGGAGGGATTAC
TCATCGATAAGCAAGCCACTTTGGCCGGGTTAAAGGGGTTGTTGCAAGATTTCTTCGCTTATTTTTTTGGTCGGGAATTG
GCATTAAGATTCAGACCTTCCTATTTCCCTTTTACTGAACCTTCCGCAGAAGTGGATATAGAATGTACTCAATGTAATGG
TAAGGGTTGCCGCTCTTGCAAGTTTACAGGCTGGCTTGAGGTATTGGGTTGTGGTATGGTCCACCCTAACGTTTTAATCG
CAGTGAACATTGATCCGAATGAGTATCATGGATGGGCTTTTGGCATGGGTATGGACAGATTGGCAATGCTTTATTATGGC
ATAGATGATTTGCGTATGTTATTTGAAAATGATTTAACTTTTTTACGGCAGTTTTAA

Upstream 100 bases:

>100_bases
AAAAATCTCAATTCGGAATAGCAATCTTAAATAAAAGCCGGTATGTAAAATAAAAATTATTGAGGAGGCTAAGGCCTCCT
TTTTACTTGTGGGTAGAACT

Downstream 100 bases:

>100_bases
CCATTATAAAAATAATGAGTCCCGGATTTTATTTTTATGTTTCTTTATGATTGTCTTTCATATGGTACATAAATGATACA
GATTAGACCAAATTGTAGCC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MLVLINTIQEQASEAIKQATDIVALEQIRVDFLGKKGKLTELLKGLANLSAEEKPKVGQLVNQAKQGISALIETKMIELK
EKQLLAKLAAEQIDVTLPGRNHSTGSLHPVTQVKHRINDYFSRLGFDIVEGPEVETEFYNFEALNIPGHHPARAMHDTFY
FGDGRLLRTHTSPVQIRTMEQRKPPFRLIAPGRVYRCDSDVTHTPMFHQVEGLLIDKQATLAGLKGLLQDFFAYFFGREL
ALRFRPSYFPFTEPSAEVDIECTQCNGKGCRSCKFTGWLEVLGCGMVHPNVLIAVNIDPNEYHGWAFGMGMDRLAMLYYG
IDDLRMLFENDLTFLRQF

Sequences:

>Translated_338_residues
MLVLINTIQEQASEAIKQATDIVALEQIRVDFLGKKGKLTELLKGLANLSAEEKPKVGQLVNQAKQGISALIETKMIELK
EKQLLAKLAAEQIDVTLPGRNHSTGSLHPVTQVKHRINDYFSRLGFDIVEGPEVETEFYNFEALNIPGHHPARAMHDTFY
FGDGRLLRTHTSPVQIRTMEQRKPPFRLIAPGRVYRCDSDVTHTPMFHQVEGLLIDKQATLAGLKGLLQDFFAYFFGREL
ALRFRPSYFPFTEPSAEVDIECTQCNGKGCRSCKFTGWLEVLGCGMVHPNVLIAVNIDPNEYHGWAFGMGMDRLAMLYYG
IDDLRMLFENDLTFLRQF
>Mature_338_residues
MLVLINTIQEQASEAIKQATDIVALEQIRVDFLGKKGKLTELLKGLANLSAEEKPKVGQLVNQAKQGISALIETKMIELK
EKQLLAKLAAEQIDVTLPGRNHSTGSLHPVTQVKHRINDYFSRLGFDIVEGPEVETEFYNFEALNIPGHHPARAMHDTFY
FGDGRLLRTHTSPVQIRTMEQRKPPFRLIAPGRVYRCDSDVTHTPMFHQVEGLLIDKQATLAGLKGLLQDFFAYFFGREL
ALRFRPSYFPFTEPSAEVDIECTQCNGKGCRSCKFTGWLEVLGCGMVHPNVLIAVNIDPNEYHGWAFGMGMDRLAMLYYG
IDDLRMLFENDLTFLRQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=290, Percent_Identity=32.4137931034483, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4758340, Length=272, Percent_Identity=31.25, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1788007, Length=327, Percent_Identity=57.4923547400612, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17508957, Length=274, Percent_Identity=30.6569343065693, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32563657, Length=274, Percent_Identity=30.6569343065693, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32566635, Length=285, Percent_Identity=32.280701754386, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321087, Length=271, Percent_Identity=30.2583025830258, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6325304, Length=101, Percent_Identity=40.5940594059406, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI18858079, Length=307, Percent_Identity=28.3387622149837, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17137424, Length=299, Percent_Identity=28.4280936454849, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 38265; Mature: 38265

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVLINTIQEQASEAIKQATDIVALEQIRVDFLGKKGKLTELLKGLANLSAEEKPKVGQL
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHH
VNQAKQGISALIETKMIELKEKQLLAKLAAEQIDVTLPGRNHSTGSLHPVTQVKHRINDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHH
FSRLGFDIVEGPEVETEFYNFEALNIPGHHPARAMHDTFYFGDGRLLRTHTSPVQIRTME
HHHCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHCEEEECCCEEEEECCCCEEEEEHH
QRKPPFRLIAPGRVYRCDSDVTHTPMFHQVEGLLIDKQATLAGLKGLLQDFFAYFFGREL
HCCCCEEEECCCEEEECCCCCCCCCHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHCCCE
ALRFRPSYFPFTEPSAEVDIECTQCNGKGCRSCKFTGWLEVLGCGMVHPNVLIAVNIDPN
EEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCC
EYHGWAFGMGMDRLAMLYYGIDDLRMLFENDLTFLRQF
CCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLVLINTIQEQASEAIKQATDIVALEQIRVDFLGKKGKLTELLKGLANLSAEEKPKVGQL
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHH
VNQAKQGISALIETKMIELKEKQLLAKLAAEQIDVTLPGRNHSTGSLHPVTQVKHRINDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHH
FSRLGFDIVEGPEVETEFYNFEALNIPGHHPARAMHDTFYFGDGRLLRTHTSPVQIRTME
HHHCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHCEEEECCCEEEEECCCCEEEEEHH
QRKPPFRLIAPGRVYRCDSDVTHTPMFHQVEGLLIDKQATLAGLKGLLQDFFAYFFGREL
HCCCCEEEECCCEEEECCCCCCCCCHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHCCCE
ALRFRPSYFPFTEPSAEVDIECTQCNGKGCRSCKFTGWLEVLGCGMVHPNVLIAVNIDPN
EEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCC
EYHGWAFGMGMDRLAMLYYGIDDLRMLFENDLTFLRQF
CCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA