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The map label for this gene is cqsA [H]

Identifier: 148358531

GI number: 148358531

Start: 3257667

End: 3258917

Strand: Direct

Name: cqsA [H]

Synonym: LPC_0402

Alternate gene names: 148358531

Gene position: 3257667-3258917 (Clockwise)

Preceding gene: 148358536

Following gene: 148358529

Centisome position: 91.09

GC content: 38.93

Gene sequence:

>1251_bases
ATGAGCAATACAGAATACCAAACAATTGATAACGAGACTCAATATATCAATGGAAAAGAAAAACATCAAAACCATGCTTA
TCCTGAGTACATCAATCATTCCATGGAAAAATATTACGAGGGACGAGTCAATAAATCCTGGCAAGGGAAACACATACTTA
AAGGCAAAACCCCGACTGCAAATTCACTGATTTTTTCGAGCAATGATTATTTAAACATCAGTCAACATCCGCAATTAATT
AAAGCGCAAATTGCTGCGATGCAAAAATATGGTAATGGTCAAATGCAATCTGCTGTATTTTTAAATAACAGCTCTTTTTT
GCTCAGCCAATGTGAAAATAAATTCAGTTCCTTTTTAAATTATTCTGCGGCCCTGCTCACTCAATCAGGCTGGAGCGCTA
ACGTTGGTCTCATACAAACCCTGGCCAAGTACAATACACCAGTGTATCTGGATTTTTATGCCCATATGTCTTTTTGGTCT
GGTGTAAAAGCAGCAAGAGCCAAACCTATACCGTTTCTACATAATTCAGTCGATTCACTAAAAAAACGAATTGAACACCA
TGGACCAGGAATAATTGCAGTAGATTCTATTTATAGTACGCTGGGTACGATTAGTCCATTGACTGCCTATGCTGACATTG
CAAGAAAATTCAAATGTTTACTCATCGTCGATGAATCTCATTCATTAGGTACGCATGGCTCGCAAGGCAAGGGAATGGTG
GCTAAACTGGGATTATCAAGCCAAATAGACATCATAACAGCAAGCCTGGCTAAAGCCTTATCAGGACGTGGAGGCATCAT
TACAGGGGATAGGCAACTTATAGAATATATCAGATATTCATCTCTGCCTTCTATTTTTAGTTCTGCTTTAATGCCTCATG
ATTTAGCAGGATTTAGCGCATCATTAGAAATTATTACTCAAGAAGAATGGAGAAGAGCAAAATTACAAACCAATGCTGAT
TTTTTACGAGAAGCCTTATCATGCGAAGGAATTAATATAGGAAGTAGTGGCTCTCAAATAGTCCCTTTAGTTACAGGCAG
CGAGGCTAACACGCTTTGGTTAAGGGATGAGTTAGAAAAAGAAGACCTCTTTGGAGCCGTGTTCTGTTCACCCGCCACTC
CCAAAAATAAATGCCTGATTCGTTTATCCATCAGTGCGCATCACGAAAAATCGGACTTGCTCAGAGTGATTGAGTGCTTG
GCCAAACTTGCAAGAAAAAGACCCGATATTCCTTTGTTTAAGGGTAATTAA

Upstream 100 bases:

>100_bases
TAAAGTCATTAACTTAATACTTAGACTTTTACTGAAAAGATCCTTCCACTATCAACCATTGTGCTAAACTGCACACAAGG
GAAAAGAATCTGAGTTATGG

Downstream 100 bases:

>100_bases
CAGGACTTCTAATTATTGGCAGCTCATTTGAACAGTTACCATTTCAAATAAGAATTATGTGCTCTTTATATAGAATTCCT
ATTAATTCAGAAACATTTCT

Product: hypothetical protein

Products: Acetyl-CoA; Glycine [C]

Alternate protein names: Cholerae quorum-sensing autoinducer [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSNTEYQTIDNETQYINGKEKHQNHAYPEYINHSMEKYYEGRVNKSWQGKHILKGKTPTANSLIFSSNDYLNISQHPQLI
KAQIAAMQKYGNGQMQSAVFLNNSSFLLSQCENKFSSFLNYSAALLTQSGWSANVGLIQTLAKYNTPVYLDFYAHMSFWS
GVKAARAKPIPFLHNSVDSLKKRIEHHGPGIIAVDSIYSTLGTISPLTAYADIARKFKCLLIVDESHSLGTHGSQGKGMV
AKLGLSSQIDIITASLAKALSGRGGIITGDRQLIEYIRYSSLPSIFSSALMPHDLAGFSASLEIITQEEWRRAKLQTNAD
FLREALSCEGINIGSSGSQIVPLVTGSEANTLWLRDELEKEDLFGAVFCSPATPKNKCLIRLSISAHHEKSDLLRVIECL
AKLARKRPDIPLFKGN

Sequences:

>Translated_416_residues
MSNTEYQTIDNETQYINGKEKHQNHAYPEYINHSMEKYYEGRVNKSWQGKHILKGKTPTANSLIFSSNDYLNISQHPQLI
KAQIAAMQKYGNGQMQSAVFLNNSSFLLSQCENKFSSFLNYSAALLTQSGWSANVGLIQTLAKYNTPVYLDFYAHMSFWS
GVKAARAKPIPFLHNSVDSLKKRIEHHGPGIIAVDSIYSTLGTISPLTAYADIARKFKCLLIVDESHSLGTHGSQGKGMV
AKLGLSSQIDIITASLAKALSGRGGIITGDRQLIEYIRYSSLPSIFSSALMPHDLAGFSASLEIITQEEWRRAKLQTNAD
FLREALSCEGINIGSSGSQIVPLVTGSEANTLWLRDELEKEDLFGAVFCSPATPKNKCLIRLSISAHHEKSDLLRVIECL
AKLARKRPDIPLFKGN
>Mature_415_residues
SNTEYQTIDNETQYINGKEKHQNHAYPEYINHSMEKYYEGRVNKSWQGKHILKGKTPTANSLIFSSNDYLNISQHPQLIK
AQIAAMQKYGNGQMQSAVFLNNSSFLLSQCENKFSSFLNYSAALLTQSGWSANVGLIQTLAKYNTPVYLDFYAHMSFWSG
VKAARAKPIPFLHNSVDSLKKRIEHHGPGIIAVDSIYSTLGTISPLTAYADIARKFKCLLIVDESHSLGTHGSQGKGMVA
KLGLSSQIDIITASLAKALSGRGGIITGDRQLIEYIRYSSLPSIFSSALMPHDLAGFSASLEIITQEEWRRAKLQTNADF
LREALSCEGINIGSSGSQIVPLVTGSEANTLWLRDELEKEDLFGAVFCSPATPKNKCLIRLSISAHHEKSDLLRVIECLA
KLARKRPDIPLFKGN

Specific function: Required for the synthesis of the quorum-sensing autoinducer CAI-1 ((S)-3-hydroxytridecan-4-one) which probably functions as an intragenus signal [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI83977440, Length=392, Percent_Identity=29.8469387755102, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI83977444, Length=392, Percent_Identity=29.8469387755102, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI83977442, Length=392, Percent_Identity=29.8469387755102, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI7657118, Length=374, Percent_Identity=28.3422459893048, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI284448556, Length=357, Percent_Identity=28.0112044817927, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI4502025, Length=333, Percent_Identity=29.1291291291291, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI40316939, Length=333, Percent_Identity=29.1291291291291, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI119220554, Length=317, Percent_Identity=27.7602523659306, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI4758668, Length=333, Percent_Identity=26.7267267267267, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI5454084, Length=385, Percent_Identity=24.1558441558442, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1790046, Length=348, Percent_Identity=31.0344827586207, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1786993, Length=342, Percent_Identity=25.7309941520468, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71994529, Length=372, Percent_Identity=28.494623655914, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI32566772, Length=370, Percent_Identity=24.3243243243243, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17560912, Length=360, Percent_Identity=25.2777777777778, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17560914, Length=358, Percent_Identity=25.4189944134078, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71982625, Length=245, Percent_Identity=24.8979591836735, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71982617, Length=245, Percent_Identity=24.8979591836735, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320438, Length=311, Percent_Identity=25.4019292604502, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320267, Length=365, Percent_Identity=24.1095890410959, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323954, Length=261, Percent_Identity=22.9885057471264, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24662918, Length=346, Percent_Identity=27.7456647398844, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI17137420, Length=290, Percent_Identity=30.6896551724138, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24653280, Length=265, Percent_Identity=25.2830188679245, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24653276, Length=265, Percent_Identity=25.2830188679245, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24653278, Length=265, Percent_Identity=25.2830188679245, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17136286, Length=324, Percent_Identity=25, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.3.1.29 [C]

Molecular weight: Translated: 46052; Mature: 45921

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTEYQTIDNETQYINGKEKHQNHAYPEYINHSMEKYYEGRVNKSWQGKHILKGKTPTA
CCCCCCCCCCCCHHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCC
NSLIFSSNDYLNISQHPQLIKAQIAAMQKYGNGQMQSAVFLNNSSFLLSQCENKFSSFLN
CEEEECCCCEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
YSAALLTQSGWSANVGLIQTLAKYNTPVYLDFYAHMSFWSGVKAARAKPIPFLHNSVDSL
HHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHCHHHHCCCCCCHHHCCHHHH
KKRIEHHGPGIIAVDSIYSTLGTISPLTAYADIARKFKCLLIVDESHSLGTHGSQGKGMV
HHHHHHCCCCEEEHHHHHHHHHCCCHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCEE
AKLGLSSQIDIITASLAKALSGRGGIITGDRQLIEYIRYSSLPSIFSSALMPHDLAGFSA
EECCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCHHHHHHHCCCCHHCCCCC
SLEIITQEEWRRAKLQTNADFLREALSCEGINIGSSGSQIVPLVTGSEANTLWLRDELEK
EEEEEEHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEHHHCCH
EDLFGAVFCSPATPKNKCLIRLSISAHHEKSDLLRVIECLAKLARKRPDIPLFKGN
HHCCEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SNTEYQTIDNETQYINGKEKHQNHAYPEYINHSMEKYYEGRVNKSWQGKHILKGKTPTA
CCCCCCCCCCCHHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCC
NSLIFSSNDYLNISQHPQLIKAQIAAMQKYGNGQMQSAVFLNNSSFLLSQCENKFSSFLN
CEEEECCCCEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
YSAALLTQSGWSANVGLIQTLAKYNTPVYLDFYAHMSFWSGVKAARAKPIPFLHNSVDSL
HHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHCHHHHCCCCCCHHHCCHHHH
KKRIEHHGPGIIAVDSIYSTLGTISPLTAYADIARKFKCLLIVDESHSLGTHGSQGKGMV
HHHHHHCCCCEEEHHHHHHHHHCCCHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCEE
AKLGLSSQIDIITASLAKALSGRGGIITGDRQLIEYIRYSSLPSIFSSALMPHDLAGFSA
EECCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCHHHHHHHCCCCHHCCCCC
SLEIITQEEWRRAKLQTNADFLREALSCEGINIGSSGSQIVPLVTGSEANTLWLRDELEK
EEEEEEHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEHHHCCH
EDLFGAVFCSPATPKNKCLIRLSISAHHEKSDLLRVIECLAKLARKRPDIPLFKGN
HHCCEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5-phosphate mol per mol subunit, enzyme, [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 12 {butyryl-CoA}} 0.08 {propionyl-CoA}} 0.059 {acetyl-CoA}} [C]

Substrates: L-2-Amino-3-oxobutanoate; Coenzyme A [C]

Specific reaction: L-2-Amino-3-oxobutanoate + Coenzyme A --> Acetyl-CoA + Glycine [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 2-Cyclohexanedione; 2, 3-Butanedione; 4-(Oxoacetyl) phenoxyacetic acid; Aminomalonic acid; Aminomethyl phosphonic acid; Aminooxyacetate; Ba2+; Ca2+; Cd2+; CoA; Cu2+; DTT; Glutathione; Hg2+; Hydroxy l amine; IAA; L-Cysteine; Mn2+; PCMB, NEM, glycinemeth

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301; 12176318 [H]