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The map label for this gene is cca [H]

Identifier: 148358518

GI number: 148358518

Start: 3269919

End: 3271151

Strand: Reverse

Name: cca [H]

Synonym: LPC_0388

Alternate gene names: 148358518

Gene position: 3271151-3269919 (Counterclockwise)

Preceding gene: 148358516

Following gene: 148358519

Centisome position: 91.46

GC content: 41.69

Gene sequence:

>1233_bases
ATGAAAGTCTACTTGGTAGGCGGTGCAGTTCGTGATCGATTATTAGGAATTCCTGTCCAGGAACAGGATTGGGTCGTCGT
CGGAGCAACGCCGGAAGAATTATTAAAAAGAAGATATAGGCAAGTTGGTCGGGATTTCCCCGTTTTTTTGCATCCTGAAA
CGAAAGAGGAATATGCATTAGCAAGAACAGAGCGTAAATCAGCTCCAGGATATTATGGATTCATTTGTGATTTTAGTGAA
TCAGTGACATTGGAAGAGGATTTGGCACGACGAGATTTAACGATTAATGCCATGGCTATGGATGAACAGGGAAATTTAAT
TGATCCATATCAGGGACAGCGAGATCTCGAAGAGAAATTATTACGTCATGTGTCCCCCGCTTTTGCAGAAGATCCTGTTC
GGGTTTTACGTGTTGCCCGTTTTGCCAGTCGGTTTCATCATCTTGGATTTAGAGTAGCCAATGAGACTCGTTTATTAATG
TATTCCATGGTTAAACAAGGTGAATTAGCTCATTTGATCCCTGAGCGTGTTTGGCAGGAATGGCAAAAAAGTCTTGAAGA
AAAAAACCCTGAGCAATTCATTCTTTCTTTACGTTCCTGTGATGCATTAAGAGTGATTTTGCCAGAAATTAACTCATTAT
TTGGTGTGCCTAATCCGTATCAATACCATCAAGAAATAGATACGGGCATTCATTCATTAATGGCATTACGGGCATCATCT
GAGCTTAGCGAAGAACCATTAGTACGATTTGCTGCTTTGGTTCATGATTTAGGCAAAGCCTCGACACCAATTCAGGCTTG
GCCTAAACACCATGGGCATGAGGAGGAGGGTACCAAGCTGATTCGAGCTTTATGTACCCGCTTGCGCATTCCTAATGATT
ATCGCGATTTGGCCGTTACAGTGGCGCGAGCTCATTTGAATATTCATAGGGTATGCGAATTGCGTCCTAACACAGTCGTC
AAGCTTTTGGAGCAGGTAGATGCCTTTAGACGGCCTCAACTATTTCATAAAATATTGATTGCTTGCCAAGCAGATGCCGA
GAGTTGTGGCAAAACAGTGGTCTATCGCCAAACTCAATTATGGAATGAAATATTATCTGAATGTGTTAAAGTGAAGCCAC
AAACTTTTATCGTACAAGGTTATGAAGGTAAAGCGATTAAGGAGGCCATGCATCAAAGTCGTGTGGCTTGTGTAGAGCGA
ATTATGACTTCTTGGAAATCAAATGAAAAATAA

Upstream 100 bases:

>100_bases
CGAGCAGATTGCTGCTTATTGATTCGTCTTTTACTCATAGTGTCAATCTATACTATAATTGATGCGATTAATAATACAGT
ATGAAGGTAAATCATATTTA

Downstream 100 bases:

>100_bases
TCAAAATTTAATTTGGATTGATTTGGAAATGACTGGCCTTGAGCCAGAACAAGATAGAATTATAGAAATGGCCACGATTG
TAACGGATCCACAACTTAAC

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKVYLVGGAVRDRLLGIPVQEQDWVVVGATPEELLKRRYRQVGRDFPVFLHPETKEEYALARTERKSAPGYYGFICDFSE
SVTLEEDLARRDLTINAMAMDEQGNLIDPYQGQRDLEEKLLRHVSPAFAEDPVRVLRVARFASRFHHLGFRVANETRLLM
YSMVKQGELAHLIPERVWQEWQKSLEEKNPEQFILSLRSCDALRVILPEINSLFGVPNPYQYHQEIDTGIHSLMALRASS
ELSEEPLVRFAALVHDLGKASTPIQAWPKHHGHEEEGTKLIRALCTRLRIPNDYRDLAVTVARAHLNIHRVCELRPNTVV
KLLEQVDAFRRPQLFHKILIACQADAESCGKTVVYRQTQLWNEILSECVKVKPQTFIVQGYEGKAIKEAMHQSRVACVER
IMTSWKSNEK

Sequences:

>Translated_410_residues
MKVYLVGGAVRDRLLGIPVQEQDWVVVGATPEELLKRRYRQVGRDFPVFLHPETKEEYALARTERKSAPGYYGFICDFSE
SVTLEEDLARRDLTINAMAMDEQGNLIDPYQGQRDLEEKLLRHVSPAFAEDPVRVLRVARFASRFHHLGFRVANETRLLM
YSMVKQGELAHLIPERVWQEWQKSLEEKNPEQFILSLRSCDALRVILPEINSLFGVPNPYQYHQEIDTGIHSLMALRASS
ELSEEPLVRFAALVHDLGKASTPIQAWPKHHGHEEEGTKLIRALCTRLRIPNDYRDLAVTVARAHLNIHRVCELRPNTVV
KLLEQVDAFRRPQLFHKILIACQADAESCGKTVVYRQTQLWNEILSECVKVKPQTFIVQGYEGKAIKEAMHQSRVACVER
IMTSWKSNEK
>Mature_410_residues
MKVYLVGGAVRDRLLGIPVQEQDWVVVGATPEELLKRRYRQVGRDFPVFLHPETKEEYALARTERKSAPGYYGFICDFSE
SVTLEEDLARRDLTINAMAMDEQGNLIDPYQGQRDLEEKLLRHVSPAFAEDPVRVLRVARFASRFHHLGFRVANETRLLM
YSMVKQGELAHLIPERVWQEWQKSLEEKNPEQFILSLRSCDALRVILPEINSLFGVPNPYQYHQEIDTGIHSLMALRASS
ELSEEPLVRFAALVHDLGKASTPIQAWPKHHGHEEEGTKLIRALCTRLRIPNDYRDLAVTVARAHLNIHRVCELRPNTVV
KLLEQVDAFRRPQLFHKILIACQADAESCGKTVVYRQTQLWNEILSECVKVKPQTFIVQGYEGKAIKEAMHQSRVACVER
IMTSWKSNEK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=408, Percent_Identity=54.4117647058823, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI87081691, Length=171, Percent_Identity=34.5029239766082, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 47198; Mature: 47198

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYLVGGAVRDRLLGIPVQEQDWVVVGATPEELLKRRYRQVGRDFPVFLHPETKEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
ARTERKSAPGYYGFICDFSESVTLEEDLARRDLTINAMAMDEQGNLIDPYQGQRDLEEKL
HHHHHCCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHH
LRHVSPAFAEDPVRVLRVARFASRFHHLGFRVANETRLLMYSMVKQGELAHLIPERVWQE
HHHCCCHHCCCHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHCCCHHHHHHHHHHHH
WQKSLEEKNPEQFILSLRSCDALRVILPEINSLFGVPNPYQYHQEIDTGIHSLMALRASS
HHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELSEEPLVRFAALVHDLGKASTPIQAWPKHHGHEEEGTKLIRALCTRLRIPNDYRDLAVT
CCCHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
VARAHLNIHRVCELRPNTVVKLLEQVDAFRRPQLFHKILIACQADAESCGKTVVYRQTQL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHCCEEHHHHHHH
WNEILSECVKVKPQTFIVQGYEGKAIKEAMHQSRVACVERIMTSWKSNEK
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVYLVGGAVRDRLLGIPVQEQDWVVVGATPEELLKRRYRQVGRDFPVFLHPETKEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
ARTERKSAPGYYGFICDFSESVTLEEDLARRDLTINAMAMDEQGNLIDPYQGQRDLEEKL
HHHHHCCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHH
LRHVSPAFAEDPVRVLRVARFASRFHHLGFRVANETRLLMYSMVKQGELAHLIPERVWQE
HHHCCCHHCCCHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHCCCHHHHHHHHHHHH
WQKSLEEKNPEQFILSLRSCDALRVILPEINSLFGVPNPYQYHQEIDTGIHSLMALRASS
HHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELSEEPLVRFAALVHDLGKASTPIQAWPKHHGHEEEGTKLIRALCTRLRIPNDYRDLAVT
CCCHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
VARAHLNIHRVCELRPNTVVKLLEQVDAFRRPQLFHKILIACQADAESCGKTVVYRQTQL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHCCEEHHHHHHH
WNEILSECVKVKPQTFIVQGYEGKAIKEAMHQSRVACVERIMTSWKSNEK
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA