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The map label for this gene is pbuE [H]

Identifier: 148358394

GI number: 148358394

Start: 267974

End: 269122

Strand: Direct

Name: pbuE [H]

Synonym: LPC_0259

Alternate gene names: 148358394

Gene position: 267974-269122 (Clockwise)

Preceding gene: 148358393

Following gene: 148358395

Centisome position: 7.49

GC content: 42.56

Gene sequence:

>1149_bases
ATGAAGAAAAGCCTGATTTTTTTAGCAATAGGAATGTTTACAGTAGGTTGCAATACATTTCTAATTGCTGGTCTGCTGCC
TCAAATAGGCGAAACGCTTGGGCAGCCGGTTGCGGTAACTGGGCAAGGAGTGAGTCTATTCAGTTTGACTTATCTTTTTT
CAGCGCCACTTTTTTCTCTGATTTTTGTTAATCAGCCGGTAAAGCGTATGATTCAGCTTGCGTTTACTGTCTTCATGCTT
GGCAATTTAATAACTCTACTTTCTGAAAATATCATGCTGTTTTTAATTGGAAGATCTCTGGCGGGAGTAGGAAGCGGGAT
TTTTACGCCGTTATGTATCAGCATTGCCGTTCATTTTGCCAGCCCATCTGCCAAAGGACGAATTTTAAGTTTTATCTGGA
GTGCTAACAGTGCGGGTGTAGTGTTTGGCGTTCCTGCCGGACTTTACTTATCCTCCTCGTTTCATTGGCAGTTATCGATT
GCCAGTCTTATTATTTTAAGTTTGCTTGCATTGGTTGGTTTTTCAATGCAAAACATTGACATAAGACTACCCAAACCTTC
GCCATTTGGAGGCAGGCTTCGTCTTCTGGTCGAGCCAAAAACGCTATCGGTAATTGGAATTACTTGTTTTACCGCCTTGG
CAAGTTTGGGACTATACTCGTATGTCACCCTGATTCAATCAGGATCCCCTAATTCGCTCAGTATGACGCTATTGAGTTGG
GGACTGGGAGGATTTATGGGAAGCTCACTGATTGGAGTGTGTATCGATCGAACAGGTAAACCACGGGTTATTATGGCCTT
AATTTTGGTTGGCCTCATGTTTGCTCTGATTGCCATACCATTCACCAGGAATCTGCCTTACCTGGGATTAATCCCTTTTT
TTATGTGGGGTGCTTGCGGATGGGCTATAGTGACTCCCCAGCAACACATTTTATATGAATTACATGAAAATCAGGGAACT
ATCCTTGCCGCTATCAATTCATCGGCTTTGGGCTTGGGGGCGGCTTTGGGAACTCTGCTTGGCGGCTTATTGATTTCATC
TGGATTCAGGGGAATCTATCTTCCTTTTTCTGCTGCCACTTTATTGTTTTTTGTATTGATAACTCAGCTGATAGTAATTA
ACACTTCACATAAGGTAAATAACACATGA

Upstream 100 bases:

>100_bases
CGCACTATGCAAACCAGGCCATTTTAGTAAGACCTGACTCCTACATCCAATACTCTGTGCCGCTGGATAAAATGGAATCC
TTATTTGAATTGGCACAGTT

Downstream 100 bases:

>100_bases
ACAGGCCCGTTGTCATTGTCGATCCATTATCATCAGGAATAGAGTTGGCTCCTGCCTTTAAGGCTCGGGGGATACCTGCT
ATTGCAGTCACGCTTAAACC

Product: chloramphenicol resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKKSLIFLAIGMFTVGCNTFLIAGLLPQIGETLGQPVAVTGQGVSLFSLTYLFSAPLFSLIFVNQPVKRMIQLAFTVFML
GNLITLLSENIMLFLIGRSLAGVGSGIFTPLCISIAVHFASPSAKGRILSFIWSANSAGVVFGVPAGLYLSSSFHWQLSI
ASLIILSLLALVGFSMQNIDIRLPKPSPFGGRLRLLVEPKTLSVIGITCFTALASLGLYSYVTLIQSGSPNSLSMTLLSW
GLGGFMGSSLIGVCIDRTGKPRVIMALILVGLMFALIAIPFTRNLPYLGLIPFFMWGACGWAIVTPQQHILYELHENQGT
ILAAINSSALGLGAALGTLLGGLLISSGFRGIYLPFSAATLLFFVLITQLIVINTSHKVNNT

Sequences:

>Translated_382_residues
MKKSLIFLAIGMFTVGCNTFLIAGLLPQIGETLGQPVAVTGQGVSLFSLTYLFSAPLFSLIFVNQPVKRMIQLAFTVFML
GNLITLLSENIMLFLIGRSLAGVGSGIFTPLCISIAVHFASPSAKGRILSFIWSANSAGVVFGVPAGLYLSSSFHWQLSI
ASLIILSLLALVGFSMQNIDIRLPKPSPFGGRLRLLVEPKTLSVIGITCFTALASLGLYSYVTLIQSGSPNSLSMTLLSW
GLGGFMGSSLIGVCIDRTGKPRVIMALILVGLMFALIAIPFTRNLPYLGLIPFFMWGACGWAIVTPQQHILYELHENQGT
ILAAINSSALGLGAALGTLLGGLLISSGFRGIYLPFSAATLLFFVLITQLIVINTSHKVNNT
>Mature_382_residues
MKKSLIFLAIGMFTVGCNTFLIAGLLPQIGETLGQPVAVTGQGVSLFSLTYLFSAPLFSLIFVNQPVKRMIQLAFTVFML
GNLITLLSENIMLFLIGRSLAGVGSGIFTPLCISIAVHFASPSAKGRILSFIWSANSAGVVFGVPAGLYLSSSFHWQLSI
ASLIILSLLALVGFSMQNIDIRLPKPSPFGGRLRLLVEPKTLSVIGITCFTALASLGLYSYVTLIQSGSPNSLSMTLLSW
GLGGFMGSSLIGVCIDRTGKPRVIMALILVGLMFALIAIPFTRNLPYLGLIPFFMWGACGWAIVTPQQHILYELHENQGT
ILAAINSSALGLGAALGTLLGGLLISSGFRGIYLPFSAATLLFFVLITQLIVINTSHKVNNT

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine and 6-mercaptopurine. Therefore plays a role in maintaining t

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787947, Length=332, Percent_Identity=25.6024096385542, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786595, Length=367, Percent_Identity=23.9782016348774, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40683; Mature: 40683

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSLIFLAIGMFTVGCNTFLIAGLLPQIGETLGQPVAVTGQGVSLFSLTYLFSAPLFSL
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHH
IFVNQPVKRMIQLAFTVFMLGNLITLLSENIMLFLIGRSLAGVGSGIFTPLCISIAVHFA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCHHHHHHHHHHHHHHC
SPSAKGRILSFIWSANSAGVVFGVPAGLYLSSSFHWQLSIASLIILSLLALVGFSMQNID
CCCCCCCEEEEEECCCCCCEEEECCCCEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCE
IRLPKPSPFGGRLRLLVEPKTLSVIGITCFTALASLGLYSYVTLIQSGSPNSLSMTLLSW
EECCCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHH
GLGGFMGSSLIGVCIDRTGKPRVIMALILVGLMFALIAIPFTRNLPYLGLIPFFMWGACG
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
WAIVTPQQHILYELHENQGTILAAINSSALGLGAALGTLLGGLLISSGFRGIYLPFSAAT
EEEECCCHHHHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHH
LLFFVLITQLIVINTSHKVNNT
HHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKSLIFLAIGMFTVGCNTFLIAGLLPQIGETLGQPVAVTGQGVSLFSLTYLFSAPLFSL
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHH
IFVNQPVKRMIQLAFTVFMLGNLITLLSENIMLFLIGRSLAGVGSGIFTPLCISIAVHFA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCHHHHHHHHHHHHHHC
SPSAKGRILSFIWSANSAGVVFGVPAGLYLSSSFHWQLSIASLIILSLLALVGFSMQNID
CCCCCCCEEEEEECCCCCCEEEECCCCEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCE
IRLPKPSPFGGRLRLLVEPKTLSVIGITCFTALASLGLYSYVTLIQSGSPNSLSMTLLSW
EECCCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHH
GLGGFMGSSLIGVCIDRTGKPRVIMALILVGLMFALIAIPFTRNLPYLGLIPFFMWGACG
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
WAIVTPQQHILYELHENQGTILAAINSSALGLGAALGTLLGGLLISSGFRGIYLPFSAAT
EEEECCCHHHHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHH
LLFFVLITQLIVINTSHKVNNT
HHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA