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The map label for this gene is artJ [H]

Identifier: 148358245

GI number: 148358245

Start: 106599

End: 107327

Strand: Reverse

Name: artJ [H]

Synonym: LPC_0107

Alternate gene names: 148358245

Gene position: 107327-106599 (Counterclockwise)

Preceding gene: 148358246

Following gene: 148358244

Centisome position: 3.0

GC content: 38.82

Gene sequence:

>729_bases
ATGAAACGATTCATGTTCATCTTATTGATCTTTATAAATTTCCACGCCTTTGGAAAACCACTCATTATTGGCGTTCCAGA
TTTTGCTCCACCATTTGTATCAAGATCTGGTGGAGGTGAGTTTTATTTCGGATTCAACATCGATTTGATGAACTCGATTT
GCAAGATTATTGAGGAAGAATGCCAATACAAGGGCATGAACATTCAACAGCTATATACCAATCTCAACGCAGGAAAGGTT
GATATTATGCTGGCACCTACTCCAATCGTATCCACTGCTGGCACCAATTATATCTTTAGTCTTCCCTATTTGCCGAGCAA
TGCCCAAATTGTAACCCTGAAAGCTAATGACCATATCAATGCCTTAACGGATCTAAAAGGTAAAAAAGTTGGTACTTTAA
AATACACTTTATACGGTGGCCTCCTCCAGGAGCATTTTCAGGATTATGTTGATTTGATGCAATTTTCCAAACTTCCCGAT
ATGGCAATCGCATTGTCAAATCGAGAAATCGAAGCAGTAGTGCTTAATGCCAATGCAGCGAAATACGCGATGAGCAATTC
CGCGACTGAGCTCAAATTTGTGGGAGATCCCATTCCTATAGGTAATGGCTATGGAATACTAGCCCTCAAGAATAATGCTC
CGCTCATCATGAAAATCAACAAGGCTTTATTACAAATAGAAAAAAACGGAACCTATTTGGAAATATATAATACCTATTTC
GGTGATTAG

Upstream 100 bases:

>100_bases
AGCAAATAAAAGCATCAATTAAAAAACCACATTGACTTTAATTTGACAAAATCGATTGCTCCTCCTAAATTTTAAGTACA
TCCTAAATGGACTTAAAAAC

Downstream 100 bases:

>100_bases
GATATTATCCTGAGCAATTCACCAGGCATTTCAAAAACCCGAAACTAGATCCAACTCCAGAATTCAGGTTAAACTAATAC
CAACTGAACCTGTCTCTCAT

Product: glutamine ABC transporter

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MKRFMFILLIFINFHAFGKPLIIGVPDFAPPFVSRSGGGEFYFGFNIDLMNSICKIIEEECQYKGMNIQQLYTNLNAGKV
DIMLAPTPIVSTAGTNYIFSLPYLPSNAQIVTLKANDHINALTDLKGKKVGTLKYTLYGGLLQEHFQDYVDLMQFSKLPD
MAIALSNREIEAVVLNANAAKYAMSNSATELKFVGDPIPIGNGYGILALKNNAPLIMKINKALLQIEKNGTYLEIYNTYF
GD

Sequences:

>Translated_242_residues
MKRFMFILLIFINFHAFGKPLIIGVPDFAPPFVSRSGGGEFYFGFNIDLMNSICKIIEEECQYKGMNIQQLYTNLNAGKV
DIMLAPTPIVSTAGTNYIFSLPYLPSNAQIVTLKANDHINALTDLKGKKVGTLKYTLYGGLLQEHFQDYVDLMQFSKLPD
MAIALSNREIEAVVLNANAAKYAMSNSATELKFVGDPIPIGNGYGILALKNNAPLIMKINKALLQIEKNGTYLEIYNTYF
GD
>Mature_242_residues
MKRFMFILLIFINFHAFGKPLIIGVPDFAPPFVSRSGGGEFYFGFNIDLMNSICKIIEEECQYKGMNIQQLYTNLNAGKV
DIMLAPTPIVSTAGTNYIFSLPYLPSNAQIVTLKANDHINALTDLKGKKVGTLKYTLYGGLLQEHFQDYVDLMQFSKLPD
MAIALSNREIEAVVLNANAAKYAMSNSATELKFVGDPIPIGNGYGILALKNNAPLIMKINKALLQIEKNGTYLEIYNTYF
GD

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=247, Percent_Identity=29.1497975708502, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788649, Length=254, Percent_Identity=24.0157480314961, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1787088, Length=215, Percent_Identity=26.046511627907, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787031, Length=237, Percent_Identity=27.0042194092827, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788228, Length=222, Percent_Identity=25.6756756756757, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788648, Length=225, Percent_Identity=25.7777777777778, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26888; Mature: 26888

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFMFILLIFINFHAFGKPLIIGVPDFAPPFVSRSGGGEFYFGFNIDLMNSICKIIEEE
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHH
CQYKGMNIQQLYTNLNAGKVDIMLAPTPIVSTAGTNYIFSLPYLPSNAQIVTLKANDHIN
HHHCCCCHHHHHHCCCCCEEEEEECCCCEEECCCCCEEEECCCCCCCCEEEEEECCCCCC
ALTDLKGKKVGTLKYTLYGGLLQEHFQDYVDLMQFSKLPDMAIALSNREIEAVVLNANAA
HHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCC
KYAMSNSATELKFVGDPIPIGNGYGILALKNNAPLIMKINKALLQIEKNGTYLEIYNTYF
EEEECCCCEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCEEEEEECCCCEEEEECCCC
GD
CC
>Mature Secondary Structure
MKRFMFILLIFINFHAFGKPLIIGVPDFAPPFVSRSGGGEFYFGFNIDLMNSICKIIEEE
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHH
CQYKGMNIQQLYTNLNAGKVDIMLAPTPIVSTAGTNYIFSLPYLPSNAQIVTLKANDHIN
HHHCCCCHHHHHHCCCCCEEEEEECCCCEEECCCCCEEEECCCCCCCCEEEEEECCCCCC
ALTDLKGKKVGTLKYTLYGGLLQEHFQDYVDLMQFSKLPDMAIALSNREIEAVVLNANAA
HHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCC
KYAMSNSATELKFVGDPIPIGNGYGILALKNNAPLIMKINKALLQIEKNGTYLEIYNTYF
EEEECCCCEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCEEEEEECCCCEEEEECCCC
GD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]