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The map label for this gene is hap [H]

Identifier: 148358159

GI number: 148358159

Start: 24125

End: 25801

Strand: Direct

Name: hap [H]

Synonym: LPC_0020

Alternate gene names: 148358159

Gene position: 24125-25801 (Clockwise)

Preceding gene: 148358158

Following gene: 148358160

Centisome position: 0.67

GC content: 37.98

Gene sequence:

>1677_bases
ATGTCAAAAAAAACCATGTTTTTTATTTTGTCGATATCTACTTCAAGTATTTTTGCTGCTGACAATGTAGATTTGTATCA
AGCCCCTCTCAATAGCATTAATAAATACCCCATACTACAAACACCAAAGAATGCGATTATTTTAAAGAGTTCTTCTGCCG
TTATTGATAATTCATTGCAAAAATTAAATCAAACAAAAGAAGATAATCAAATGATTGTTCGTTATCAGCAACTGTATAAA
GGAATACCTGTTATTGGCGCCCAAGTGATGATTACTAAAGGAACAGACTCAGGAGTGCAGTCCAATGACAATGCAGAGGT
GAACGGCCATTTATTGGATAATATAGAACTTAATACGAAACCGGCTATTAGTGCGCAACAAGCGAAGGAATATGCAAAAA
AATCCTATTTTCAATTTAGCCCCCAATCTAACATACAACAGGAAACAGCTGAATTACAGATTCGGCCAGACCATAATAAT
CAATTAAAGCTGGTTTATTTGGTTTCATTTAAAAGCGTGCAGCAGTCTGGTAAACCAGACTGGCCTTTTTTTGTTATTGA
TGCTCAAACAGGGGCTTTGATTAAGCAATGGAACAATATCAAAAATTATTTGGATACAGGGCCTGGAGGTAATGAGAAAG
TTCAGGAATATTGGTATGGTAAAGATGGATTACCTGCTTTGGATGTGACTCAAAATGGCAGCCAATGCGTCATGGAAAAT
TCAAAAGTTAAATTGGTTAATCTCCATTCTCAATGGGATTGGGAAAACACCATAAATACTCCTTTTGAATACGTTTGTAA
CAATAATATAGAAGAGAAAATTAATGGAGGATTTTCTCCTGGCAATGACGCGTATTATTTCGGACATGTTATTGTTGGTA
TGTACAAGGACTGGTATGGACTTAATGCCTTACAACATTCTAATGGTGCGCCAATGCAATTGGTTATGCGAGTTCATTTT
GGGCAAAACTATGATAATGCTTTTTGGGATGGGCAAGCCATGTCATTTGGAGATGGACTGGACTTTTATCCATTGGTTTC
TTTAGACGTAGCCGGTCATGAAGTGACCCATGGTTTTACAGAGCAGCATTCTGGTCTTGAGTATCATGATCAATCAGGTG
CGCTTAATGAGTCTCTATCCGATATGGCAGGACAAGCGTCAAGAGCTTATCTTTTGGAAAAAAACCCTCAGTTGTATAAC
AAAGCTTACTTACAGCCCAATGAAGTCACATGGGGTATTGGTGAAACAATAGTTCGTGATTCTTATGGCAAAGCTTTGCG
ATTCATGGATTACCCATCCTCTGATGGAAGCTCTGCAGATTGTTTAGACAAAGGTATTGCGCAAAATAATGGCAGCTATT
GTGCTATCAGTTATGATGAGGTAGTGGCCTATGCCAATACACATATCGCACTTCCTCAAGAACGCCAGAGCTTCATAGTT
CATACAGCCAGTGGTGTGTTCAATAAGGCTTTTTACTTAATGTCTAAGGATATGGGTATTAAAAACGCTTATCACATCAT
GGTTGTTGCTAACACAAAATATTGGACTCCTACGACAGACTTTAAAAATGGAGCTTGCGGAGTCATTTATGCTGCCAGGG
ATTTAAATACCGATATCAATAAGGTTAAGTCCGCTTTTGGTCAAGTAGGTATTGATACAGCCGGGTGTGTTATTTAG

Upstream 100 bases:

>100_bases
TTAAAGTACGCATCTCAAGGCAGAATTTGAAAATAAATATTGCATAATGTTTAATTTTGTGACATTATTTGCGCCATTGT
GTATTCTGTGAGAGTTCTGT

Downstream 100 bases:

>100_bases
CGGGAGCAAAAGCTCCTGGCAAATTTTTGTTTCCTGCAAGCTGTTGTTCCCTTGTGATTCATCGCGAGCTATTTTTGCTC
GCAGTGTTGTTTGCCGGGCA

Product: hemagglutinin/protease

Products: NA

Alternate protein names: HA/protease; Vibriolysin [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MSKKTMFFILSISTSSIFAADNVDLYQAPLNSINKYPILQTPKNAIILKSSSAVIDNSLQKLNQTKEDNQMIVRYQQLYK
GIPVIGAQVMITKGTDSGVQSNDNAEVNGHLLDNIELNTKPAISAQQAKEYAKKSYFQFSPQSNIQQETAELQIRPDHNN
QLKLVYLVSFKSVQQSGKPDWPFFVIDAQTGALIKQWNNIKNYLDTGPGGNEKVQEYWYGKDGLPALDVTQNGSQCVMEN
SKVKLVNLHSQWDWENTINTPFEYVCNNNIEEKINGGFSPGNDAYYFGHVIVGMYKDWYGLNALQHSNGAPMQLVMRVHF
GQNYDNAFWDGQAMSFGDGLDFYPLVSLDVAGHEVTHGFTEQHSGLEYHDQSGALNESLSDMAGQASRAYLLEKNPQLYN
KAYLQPNEVTWGIGETIVRDSYGKALRFMDYPSSDGSSADCLDKGIAQNNGSYCAISYDEVVAYANTHIALPQERQSFIV
HTASGVFNKAFYLMSKDMGIKNAYHIMVVANTKYWTPTTDFKNGACGVIYAARDLNTDINKVKSAFGQVGIDTAGCVI

Sequences:

>Translated_558_residues
MSKKTMFFILSISTSSIFAADNVDLYQAPLNSINKYPILQTPKNAIILKSSSAVIDNSLQKLNQTKEDNQMIVRYQQLYK
GIPVIGAQVMITKGTDSGVQSNDNAEVNGHLLDNIELNTKPAISAQQAKEYAKKSYFQFSPQSNIQQETAELQIRPDHNN
QLKLVYLVSFKSVQQSGKPDWPFFVIDAQTGALIKQWNNIKNYLDTGPGGNEKVQEYWYGKDGLPALDVTQNGSQCVMEN
SKVKLVNLHSQWDWENTINTPFEYVCNNNIEEKINGGFSPGNDAYYFGHVIVGMYKDWYGLNALQHSNGAPMQLVMRVHF
GQNYDNAFWDGQAMSFGDGLDFYPLVSLDVAGHEVTHGFTEQHSGLEYHDQSGALNESLSDMAGQASRAYLLEKNPQLYN
KAYLQPNEVTWGIGETIVRDSYGKALRFMDYPSSDGSSADCLDKGIAQNNGSYCAISYDEVVAYANTHIALPQERQSFIV
HTASGVFNKAFYLMSKDMGIKNAYHIMVVANTKYWTPTTDFKNGACGVIYAARDLNTDINKVKSAFGQVGIDTAGCVI
>Mature_557_residues
SKKTMFFILSISTSSIFAADNVDLYQAPLNSINKYPILQTPKNAIILKSSSAVIDNSLQKLNQTKEDNQMIVRYQQLYKG
IPVIGAQVMITKGTDSGVQSNDNAEVNGHLLDNIELNTKPAISAQQAKEYAKKSYFQFSPQSNIQQETAELQIRPDHNNQ
LKLVYLVSFKSVQQSGKPDWPFFVIDAQTGALIKQWNNIKNYLDTGPGGNEKVQEYWYGKDGLPALDVTQNGSQCVMENS
KVKLVNLHSQWDWENTINTPFEYVCNNNIEEKINGGFSPGNDAYYFGHVIVGMYKDWYGLNALQHSNGAPMQLVMRVHFG
QNYDNAFWDGQAMSFGDGLDFYPLVSLDVAGHEVTHGFTEQHSGLEYHDQSGALNESLSDMAGQASRAYLLEKNPQLYNK
AYLQPNEVTWGIGETIVRDSYGKALRFMDYPSSDGSSADCLDKGIAQNNGSYCAISYDEVVAYANTHIALPQERQSFIVH
TASGVFNKAFYLMSKDMGIKNAYHIMVVANTKYWTPTTDFKNGACGVIYAARDLNTDINKVKSAFGQVGIDTAGCVI

Specific function: May play a role in the pathogenesis of cholera. Hap nicks and activates the A subunit of cholera enterotoxin and related enterotoxins [H]

COG id: COG3227

COG function: function code E; Zinc metalloprotease (elastase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005075
- InterPro:   IPR007280
- InterPro:   IPR013856
- InterPro:   IPR001570
- InterPro:   IPR011096 [H]

Pfam domain/function: PF07504 FTP; PF03413 PepSY; PF01447 Peptidase_M4; PF02868 Peptidase_M4_C; PF04151 PPC [H]

EC number: NA

Molecular weight: Translated: 62124; Mature: 61993

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKTMFFILSISTSSIFAADNVDLYQAPLNSINKYPILQTPKNAIILKSSSAVIDNSLQ
CCCCEEEEEEEECCCEEEEECCCHHHHHHHHHCCCCCCEECCCCEEEEECCCHHHHHHHH
KLNQTKEDNQMIVRYQQLYKGIPVIGAQVMITKGTDSGVQSNDNAEVNGHLLDNIELNTK
HHHCCCCCHHHHHHHHHHHCCCCEECEEEEEECCCCCCCCCCCCCEECCEEEECEEECCC
PAISAQQAKEYAKKSYFQFSPQSNIQQETAELQIRPDHNNQLKLVYLVSFKSVQQSGKPD
CCCCHHHHHHHHHHHCEEECCCCCCCCCCEEEEEECCCCCCEEEEEEEEEHHHHHCCCCC
WPFFVIDAQTGALIKQWNNIKNYLDTGPGGNEKVQEYWYGKDGLPALDVTQNGSQCVMEN
CCEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEEEECCCCCEEEEEC
SKVKLVNLHSQWDWENTINTPFEYVCNNNIEEKINGGFSPGNDAYYFGHVIVGMYKDWYG
CCEEEEEECCCCCCCCCCCCCHHHHCCCCHHHHHCCCCCCCCCCEEEEEEHEEHHHHHHC
LNALQHSNGAPMQLVMRVHFGQNYDNAFWDGQAMSFGDGLDFYPLVSLDVAGHEVTHGFT
CCCEECCCCCCCEEEEEEECCCCCCCCEECCCCEECCCCCCCCEEEEEEECCCHHHCCHH
EQHSGLEYHDQSGALNESLSDMAGQASRAYLLEKNPQLYNKAYLQPNEVTWGIGETIVRD
HHCCCCCEECCCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHCCCCCCEEECCCHHHHHC
SYGKALRFMDYPSSDGSSADCLDKGIAQNNGSYCAISYDEVVAYANTHIALPQERQSFIV
CCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEHHHEEEECCCEEECCCCCCEEEE
HTASGVFNKAFYLMSKDMGIKNAYHIMVVANTKYWTPTTDFKNGACGVIYAARDLNTDIN
EECCHHHHHEEEEEECCCCCCCEEEEEEEECCEEECCCCCCCCCCEEEEEEECCCCCHHH
KVKSAFGQVGIDTAGCVI
HHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SKKTMFFILSISTSSIFAADNVDLYQAPLNSINKYPILQTPKNAIILKSSSAVIDNSLQ
CCCEEEEEEEECCCEEEEECCCHHHHHHHHHCCCCCCEECCCCEEEEECCCHHHHHHHH
KLNQTKEDNQMIVRYQQLYKGIPVIGAQVMITKGTDSGVQSNDNAEVNGHLLDNIELNTK
HHHCCCCCHHHHHHHHHHHCCCCEECEEEEEECCCCCCCCCCCCCEECCEEEECEEECCC
PAISAQQAKEYAKKSYFQFSPQSNIQQETAELQIRPDHNNQLKLVYLVSFKSVQQSGKPD
CCCCHHHHHHHHHHHCEEECCCCCCCCCCEEEEEECCCCCCEEEEEEEEEHHHHHCCCCC
WPFFVIDAQTGALIKQWNNIKNYLDTGPGGNEKVQEYWYGKDGLPALDVTQNGSQCVMEN
CCEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEEEECCCCCEEEEEC
SKVKLVNLHSQWDWENTINTPFEYVCNNNIEEKINGGFSPGNDAYYFGHVIVGMYKDWYG
CCEEEEEECCCCCCCCCCCCCHHHHCCCCHHHHHCCCCCCCCCCEEEEEEHEEHHHHHHC
LNALQHSNGAPMQLVMRVHFGQNYDNAFWDGQAMSFGDGLDFYPLVSLDVAGHEVTHGFT
CCCEECCCCCCCEEEEEEECCCCCCCCEECCCCEECCCCCCCCEEEEEEECCCHHHCCHH
EQHSGLEYHDQSGALNESLSDMAGQASRAYLLEKNPQLYNKAYLQPNEVTWGIGETIVRD
HHCCCCCEECCCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHCCCCCCEEECCCHHHHHC
SYGKALRFMDYPSSDGSSADCLDKGIAQNNGSYCAISYDEVVAYANTHIALPQERQSFIV
CCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEHHHEEEECCCEEECCCCCCEEEE
HTASGVFNKAFYLMSKDMGIKNAYHIMVVANTKYWTPTTDFKNGACGVIYAARDLNTDIN
EECCHHHHHEEEEEECCCCCCCEEEEEEEECCEEECCCCCCCCCCEEEEEEECCCCCHHH
KVKSAFGQVGIDTAGCVI
HHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2045361; 10952301; 2123831 [H]