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The map label for this gene is oprM [H]

Identifier: 148358158

GI number: 148358158

Start: 22493

End: 23899

Strand: Direct

Name: oprM [H]

Synonym: LPC_0019

Alternate gene names: 148358158

Gene position: 22493-23899 (Clockwise)

Preceding gene: 148358157

Following gene: 148358159

Centisome position: 0.63

GC content: 37.53

Gene sequence:

>1407_bases
ATGCTACGTCCGGTTAAAATCATTTTATTATCAGTCTTTTTAGTCGGATGCTATAGTGAGCCTTATCACAGACCCCATGT
TGAAGTAATTAATAAATGGTCGGTAAAAGACAAGAATGTTAAAAATATCGATAGCAAGAATATTCCTTACATAGCCTGGT
GGCGTGATTTTAAAGACCCCACTCTCAACCAGTTGATTGAAAGAGGGTTGATTTCCAATACAAGCCTCGGCATGTCAAGA
GGGAGCATTGAAGCGGCAGAGGGTGAGCTTAAAAAAATTCGTTATCAGTGGGTGCCTACCCTCGATCTTATGACTGGTTA
TTCAAGAAATCCGGCAACGGGTTTTCCTGGCGTTCTGATTGTTTTTATTCCTAACTATACAATAAACATCATCAATCAAA
TAAGAGAGCAAAAAAGGGCAAAATATACTCTTGCACAGGTAAAAGCAGAAGACGATGCTCTCAAACTAACAATTATTTCT
CAAATAGCGGCAAGTTATTTCACCTATCTTGCAGAAATAGAACGCAAAGAATTTTTACAGACATTAGCCAATGATCTAAC
TCAGTTGGCAAAGGTTGCCAATAAAGTTTATCAGGGGGGATTAAGTTCTGAAATTGAGCAGGAAGAGCTGTATAGTCAGG
TCAATTTGATACAAGGCGAGCTCGAGGTCACAGAGCGTAATATCGTTATAAGTCGCAATGCTCTCCGGTATTTACTAAAC
CAAAATCCAGGGGAAATAAAAACAGTTAAAAAATTATTACAACTTAAAAATCGTAAATTAATACCTGGTTCTTTACCGCT
AACAGTTCTTGAAAATCGGCCTGATATGCAAGTGGCTGAAAATCGTTTGCGTGCTTCACATGAAGGAATTGGTGTTGCAG
AAAGTAATCTTTTGCCTACTATACAATTAGATTATATTGGTGGTTTGGCTGCTGGAGATAGCCGCTACGCATTACCGAAA
AAAACGGTGAAATTTAATGATCAATTGTTGAAAGCCCCCATTATTGATATGCCCGTTTTAGGTGCAGTAGCTAAGGCTAA
AGGCGTAAATAAGGTTTCTTATTATAATTATATTGATACTTTACAAAAAGCATTAAGAGATACGACCAATGCCTTATCAG
AAAATGAGCGTTTAACCAATAAGCTCAAACAAACTGCGTCGGCTCAAAAACATTTGGCAAAAGCGTATAATTTGAACGAC
AGGTTATATCAGACAGGTATTCAAAGTTATATAGAAACCTTAAATAGCAAAATTTCGCTTGATAAAATCAACATCAGCTT
AAATCAGGATAAACTGCAAGAGCTACTAAGCATTGTACGTTTATATCAAGAGCTGGCTGGAGGTTATCGCGCCAACTTGG
AGTCTGAAATAAAATCTTCAGGCAGAGCAACCAGAACTCGGTTATAA

Upstream 100 bases:

>100_bases
GATTATTATCAACTATTTCAACAGGTTCAAAAAGATTTGGTGCACTTAAAAGATTTTGTTGCAGGGGAGCAAACAATTGA
TCTTAGTGAGGGAGTAAAGC

Downstream 100 bases:

>100_bases
ATTATTAATTACGAATAGGGGTGTATTTCATTTTTCATAGTACTCGGTTTATACAAATAGCGTTCTTCTTCTATAAAGCC
TCCCCGACCCAGTCTCTGAT

Product: outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MLRPVKIILLSVFLVGCYSEPYHRPHVEVINKWSVKDKNVKNIDSKNIPYIAWWRDFKDPTLNQLIERGLISNTSLGMSR
GSIEAAEGELKKIRYQWVPTLDLMTGYSRNPATGFPGVLIVFIPNYTINIINQIREQKRAKYTLAQVKAEDDALKLTIIS
QIAASYFTYLAEIERKEFLQTLANDLTQLAKVANKVYQGGLSSEIEQEELYSQVNLIQGELEVTERNIVISRNALRYLLN
QNPGEIKTVKKLLQLKNRKLIPGSLPLTVLENRPDMQVAENRLRASHEGIGVAESNLLPTIQLDYIGGLAAGDSRYALPK
KTVKFNDQLLKAPIIDMPVLGAVAKAKGVNKVSYYNYIDTLQKALRDTTNALSENERLTNKLKQTASAQKHLAKAYNLND
RLYQTGIQSYIETLNSKISLDKINISLNQDKLQELLSIVRLYQELAGGYRANLESEIKSSGRATRTRL

Sequences:

>Translated_468_residues
MLRPVKIILLSVFLVGCYSEPYHRPHVEVINKWSVKDKNVKNIDSKNIPYIAWWRDFKDPTLNQLIERGLISNTSLGMSR
GSIEAAEGELKKIRYQWVPTLDLMTGYSRNPATGFPGVLIVFIPNYTINIINQIREQKRAKYTLAQVKAEDDALKLTIIS
QIAASYFTYLAEIERKEFLQTLANDLTQLAKVANKVYQGGLSSEIEQEELYSQVNLIQGELEVTERNIVISRNALRYLLN
QNPGEIKTVKKLLQLKNRKLIPGSLPLTVLENRPDMQVAENRLRASHEGIGVAESNLLPTIQLDYIGGLAAGDSRYALPK
KTVKFNDQLLKAPIIDMPVLGAVAKAKGVNKVSYYNYIDTLQKALRDTTNALSENERLTNKLKQTASAQKHLAKAYNLND
RLYQTGIQSYIETLNSKISLDKINISLNQDKLQELLSIVRLYQELAGGYRANLESEIKSSGRATRTRL
>Mature_468_residues
MLRPVKIILLSVFLVGCYSEPYHRPHVEVINKWSVKDKNVKNIDSKNIPYIAWWRDFKDPTLNQLIERGLISNTSLGMSR
GSIEAAEGELKKIRYQWVPTLDLMTGYSRNPATGFPGVLIVFIPNYTINIINQIREQKRAKYTLAQVKAEDDALKLTIIS
QIAASYFTYLAEIERKEFLQTLANDLTQLAKVANKVYQGGLSSEIEQEELYSQVNLIQGELEVTERNIVISRNALRYLLN
QNPGEIKTVKKLLQLKNRKLIPGSLPLTVLENRPDMQVAENRLRASHEGIGVAESNLLPTIQLDYIGGLAAGDSRYALPK
KTVKFNDQLLKAPIIDMPVLGAVAKAKGVNKVSYYNYIDTLQKALRDTTNALSENERLTNKLKQTASAQKHLAKAYNLND
RLYQTGIQSYIETLNSKISLDKINISLNQDKLQELLSIVRLYQELAGGYRANLESEIKSSGRATRTRL

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=486, Percent_Identity=23.4567901234568, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI2367348, Length=434, Percent_Identity=19.1244239631336, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52879; Mature: 52879

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRPVKIILLSVFLVGCYSEPYHRPHVEVINKWSVKDKNVKNIDSKNIPYIAWWRDFKDP
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCC
TLNQLIERGLISNTSLGMSRGSIEAAEGELKKIRYQWVPTLDLMTGYSRNPATGFPGVLI
HHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCEEE
VFIPNYTINIINQIREQKRAKYTLAQVKAEDDALKLTIISQIAASYFTYLAEIERKEFLQ
EEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
TLANDLTQLAKVANKVYQGGLSSEIEQEELYSQVNLIQGELEVTERNIVISRNALRYLLN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHEEEECCEEEEHHHHHHHHC
QNPGEIKTVKKLLQLKNRKLIPGSLPLTVLENRPDMQVAENRLRASHEGIGVAESNLLPT
CCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCE
IQLDYIGGLAAGDSRYALPKKTVKFNDQLLKAPIIDMPVLGAVAKAKGVNKVSYYNYIDT
EEEECCCCCCCCCCCCCCCCCHHHHCHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
LQKALRDTTNALSENERLTNKLKQTASAQKHLAKAYNLNDRLYQTGIQSYIETLNSKISL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEE
DKINISLNQDKLQELLSIVRLYQELAGGYRANLESEIKSSGRATRTRL
EEEEEEECHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHCCCCHHHCCC
>Mature Secondary Structure
MLRPVKIILLSVFLVGCYSEPYHRPHVEVINKWSVKDKNVKNIDSKNIPYIAWWRDFKDP
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCC
TLNQLIERGLISNTSLGMSRGSIEAAEGELKKIRYQWVPTLDLMTGYSRNPATGFPGVLI
HHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCEEE
VFIPNYTINIINQIREQKRAKYTLAQVKAEDDALKLTIISQIAASYFTYLAEIERKEFLQ
EEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
TLANDLTQLAKVANKVYQGGLSSEIEQEELYSQVNLIQGELEVTERNIVISRNALRYLLN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHEEEECCEEEEHHHHHHHHC
QNPGEIKTVKKLLQLKNRKLIPGSLPLTVLENRPDMQVAENRLRASHEGIGVAESNLLPT
CCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCE
IQLDYIGGLAAGDSRYALPKKTVKFNDQLLKAPIIDMPVLGAVAKAKGVNKVSYYNYIDT
EEEECCCCCCCCCCCCCCCCCHHHHCHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
LQKALRDTTNALSENERLTNKLKQTASAQKHLAKAYNLNDRLYQTGIQSYIETLNSKISL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEE
DKINISLNQDKLQELLSIVRLYQELAGGYRANLESEIKSSGRATRTRL
EEEEEEECHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]