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The map label for this gene is dam30 [H]

Identifier: 148285068

GI number: 148285068

Start: 1911401

End: 1912207

Strand: Direct

Name: dam30 [H]

Synonym: OTBS_1978

Alternate gene names: 148285068

Gene position: 1911401-1912207 (Clockwise)

Preceding gene: 148285063

Following gene: 148285072

Centisome position: 89.86

GC content: 31.23

Gene sequence:

>807_bases
GTGTCAACGGCAATTTCTAATGAACCAAAGCCTTTTCTTCACTGGGTTGGAGGTAAAAGGAGAATTGTTAATAAATTAAT
AGAGCATCTTCCTTCAGGGCCATACTATAATTACTATGAACCATTTCTTGGAGGTGGGGCTTTATTTTTTCAAGTTAGGC
ATCTGTTTAAACAATGTTTTTTGTCTGATATTAATCTCGACTTAATTACTAGTTATCACGCTGTAAAAAAGAATCCAAAT
GAAGTCAACAGATTACTGAATCTATATCACAAAAACCATTCTGAAAATTACTACTATAAAATAAGAGACAATTATTATAG
TAATGATCCTAATGATATCACGGCAAAATTTATATATCTTAATAAATACTCTTTTAGGGGAATTTATAGGCTAAACAGAG
ATGGTACATCTGCTCAAACATTTTCTGATAAGCGATATCTTAAGCTTCATATTTGCTCTAGAATAAATAAATGTAGCAAA
CTTTTAACCGATGTATCAATTTGCGCTATGGATTTTTCGTTTATAGAACCTAAAAAAGGTGACTTCGTTTATCTTGATCC
GCCTTACCACCAATCTGGAGAACGTTTCTATACCAGAGTTCCATTTGATGAAAAAGAGCAAATCAGACTACGAGATTTTG
TTTATGAACTCAACAATAAAGGTGTTAAAATTATGCTTTCAAATAATAACACTACCTTTATTAAAGCTTTATACAAAGAC
CTCTTCATCACTCATATTGGAGTTACATACTCAATTAATGAACAACGCAATCTCGTTAATGGGCTAATAATTACCAACTA
TAATTAA

Upstream 100 bases:

>100_bases
AAAGGTTTATAAAAAAGTTCTACAACAGTTATTATTACTGGCTTTAAGGCTTATTTTCTGCTATAATTCATAGTAGAGAT
TTAATTAATCAAAAATTCTT

Downstream 100 bases:

>100_bases
ATTTAAATGTATTAACTTATTATATTATTAGTAATGAAACCGTACGCTTGCATGTATAGAAACAAGAAGTGGCCTGCTAG
GAGCCATGGTACTACATTCA

Product: site-specific DNA adenine methylase

Products: NA

Alternate protein names: M.MjaIII; Adenine-specific methyltransferase MjaIII [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MSTAISNEPKPFLHWVGGKRRIVNKLIEHLPSGPYYNYYEPFLGGGALFFQVRHLFKQCFLSDINLDLITSYHAVKKNPN
EVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYLNKYSFRGIYRLNRDGTSAQTFSDKRYLKLHICSRINKCSK
LLTDVSICAMDFSFIEPKKGDFVYLDPPYHQSGERFYTRVPFDEKEQIRLRDFVYELNNKGVKIMLSNNNTTFIKALYKD
LFITHIGVTYSINEQRNLVNGLIITNYN

Sequences:

>Translated_268_residues
MSTAISNEPKPFLHWVGGKRRIVNKLIEHLPSGPYYNYYEPFLGGGALFFQVRHLFKQCFLSDINLDLITSYHAVKKNPN
EVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYLNKYSFRGIYRLNRDGTSAQTFSDKRYLKLHICSRINKCSK
LLTDVSICAMDFSFIEPKKGDFVYLDPPYHQSGERFYTRVPFDEKEQIRLRDFVYELNNKGVKIMLSNNNTTFIKALYKD
LFITHIGVTYSINEQRNLVNGLIITNYN
>Mature_267_residues
STAISNEPKPFLHWVGGKRRIVNKLIEHLPSGPYYNYYEPFLGGGALFFQVRHLFKQCFLSDINLDLITSYHAVKKNPNE
VNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYLNKYSFRGIYRLNRDGTSAQTFSDKRYLKLHICSRINKCSKL
LTDVSICAMDFSFIEPKKGDFVYLDPPYHQSGERFYTRVPFDEKEQIRLRDFVYELNNKGVKIMLSNNNTTFIKALYKDL
FITHIGVTYSINEQRNLVNGLIITNYN

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MjaIII endonuclease (Potential) [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=255, Percent_Identity=28.6274509803922, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31648; Mature: 31516

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAISNEPKPFLHWVGGKRRIVNKLIEHLPSGPYYNYYEPFLGGGALFFQVRHLFKQCF
CCCCCCCCCCHHEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHH
LSDINLDLITSYHAVKKNPNEVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYL
HCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEEE
NKYSFRGIYRLNRDGTSAQTFSDKRYLKLHICSRINKCSKLLTDVSICAMDFSFIEPKKG
EEECCCEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCC
DFVYLDPPYHQSGERFYTRVPFDEKEQIRLRDFVYELNNKGVKIMLSNNNTTFIKALYKD
CEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
LFITHIGVTYSINEQRNLVNGLIITNYN
HHHHHCCEEEEECCCCCCCCEEEEEECC
>Mature Secondary Structure 
STAISNEPKPFLHWVGGKRRIVNKLIEHLPSGPYYNYYEPFLGGGALFFQVRHLFKQCF
CCCCCCCCCHHEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHH
LSDINLDLITSYHAVKKNPNEVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYL
HCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEEE
NKYSFRGIYRLNRDGTSAQTFSDKRYLKLHICSRINKCSKLLTDVSICAMDFSFIEPKKG
EEECCCEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCC
DFVYLDPPYHQSGERFYTRVPFDEKEQIRLRDFVYELNNKGVKIMLSNNNTTFIKALYKD
CEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
LFITHIGVTYSINEQRNLVNGLIITNYN
HHHHHCCEEEEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]