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The map label for this gene is rnhA

Identifier: 148284855

GI number: 148284855

Start: 1544127

End: 1544633

Strand: Direct

Name: rnhA

Synonym: OTBS_1582

Alternate gene names: 148284855

Gene position: 1544127-1544633 (Clockwise)

Preceding gene: 148284854

Following gene: 148284856

Centisome position: 72.59

GC content: 31.95

Gene sequence:

>507_bases
GTGACTAACTATGCTTGCTCTATCACTAACAACATTCCAGTTAAGATATATACAGATGGCGCATGTAGCCATAATCCAGG
GCCAGGAGGTTGGGGAGTATATTTAAGTTATAAGAATCATGAAAAAAAAGTTTATGGATCTAACATAAATACTACAAATA
ACCGAATGGAGTTAACAGCAACAATAGAGGCGTTGCGGCTTCTTAAGCATAGTTATAAAGTAGAATTATATACTGACAGT
CAGTATGTTCAAATGGGTATTACTAAGTGGATAAAAAAGTGGATAAAAAATAATTGGGAAACAAGCAACAAGCAATTGGT
TTGTAATGTTGATTTATGGCAGTTATTATATACTTTGATTCAAAAACATCAAGTTAGTTGGCATTGGGTGAGAGGACACT
CTGGTAATATAGGTAATGAAATTGCTGATAAATTAGCAACTAATGGAAAAATTGAAGCAATGAAAATAGCAAAAAATAAT
GAAAATACTAAGAAATTTCTTGATTAG

Upstream 100 bases:

>100_bases
ATTGCGATCAGTTAGTAGCTACAAATGAAAAGTTGAAAAATATGAAAGACATAGTAGGAAATAAAGTTGTTAGTCTTGAC
AGCTTTAGGAAGAAGATCTA

Downstream 100 bases:

>100_bases
ATATACAAGTAAGTTCGTTGGTGTTGACCAATATGGTAATAATTATTATATCTCTTTAATGAAAAGTAATTATTTAAAGA
ACAAAAATAGAATAATAATT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MTNYACSITNNIPVKIYTDGACSHNPGPGGWGVYLSYKNHEKKVYGSNINTTNNRMELTATIEALRLLKHSYKVELYTDS
QYVQMGITKWIKKWIKNNWETSNKQLVCNVDLWQLLYTLIQKHQVSWHWVRGHSGNIGNEIADKLATNGKIEAMKIAKNN
ENTKKFLD

Sequences:

>Translated_168_residues
MTNYACSITNNIPVKIYTDGACSHNPGPGGWGVYLSYKNHEKKVYGSNINTTNNRMELTATIEALRLLKHSYKVELYTDS
QYVQMGITKWIKKWIKNNWETSNKQLVCNVDLWQLLYTLIQKHQVSWHWVRGHSGNIGNEIADKLATNGKIEAMKIAKNN
ENTKKFLD
>Mature_167_residues
TNYACSITNNIPVKIYTDGACSHNPGPGGWGVYLSYKNHEKKVYGSNINTTNNRMELTATIEALRLLKHSYKVELYTDSQ
YVQMGITKWIKKWIKNNWETSNKQLVCNVDLWQLLYTLIQKHQVSWHWVRGHSGNIGNEIADKLATNGKIEAMKIAKNNE
NTKKFLD

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=143, Percent_Identity=40.5594405594406, Blast_Score=81, Evalue=4e-16,
Organism=Escherichia coli, GI1786408, Length=133, Percent_Identity=52.6315789473684, Blast_Score=146, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI115532542, Length=147, Percent_Identity=41.4965986394558, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137476, Length=147, Percent_Identity=39.4557823129252, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI45552509, Length=147, Percent_Identity=39.4557823129252, Blast_Score=80, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_ORITB (A5CEP5)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001248945.1
- ProteinModelPortal:   A5CEP5
- STRING:   A5CEP5
- GeneID:   5217961
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_1582
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- ProtClustDB:   PRK00203
- BioCyc:   OTSU357244:OTBS_1582-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 19335; Mature: 19204

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNYACSITNNIPVKIYTDGACSHNPGPGGWGVYLSYKNHEKKVYGSNINTTNNRMELTA
CCCEEEEECCCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCEEECCCCCCCCCEEEEHH
TIEALRLLKHSYKVELYTDSQYVQMGITKWIKKWIKNNWETSNKQLVCNVDLWQLLYTLI
HHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHH
QKHQVSWHWVRGHSGNIGNEIADKLATNGKIEAMKIAKNNENTKKFLD
HHCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHCC
>Mature Secondary Structure 
TNYACSITNNIPVKIYTDGACSHNPGPGGWGVYLSYKNHEKKVYGSNINTTNNRMELTA
CCEEEEECCCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCEEECCCCCCCCCEEEEHH
TIEALRLLKHSYKVELYTDSQYVQMGITKWIKKWIKNNWETSNKQLVCNVDLWQLLYTLI
HHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHH
QKHQVSWHWVRGHSGNIGNEIADKLATNGKIEAMKIAKNNENTKKFLD
HHCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA