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The map label for this gene is traD_F [H]

Identifier: 148284782

GI number: 148284782

Start: 1405801

End: 1406946

Strand: Direct

Name: traD_F [H]

Synonym: OTBS_1447

Alternate gene names: 148284782

Gene position: 1405801-1406946 (Clockwise)

Preceding gene: 148284781

Following gene: 148284784

Centisome position: 66.09

GC content: 34.47

Gene sequence:

>1146_bases
TTGGTGGCATATGGGCTGCTACTAGGGCTATTTTTAGAGGAAATATCGGCATCTTTGCTATGGGAATGGTTTTTTCCATC
GATATTTATTTTTACGCTGTTATTTGCCCCCAAATCTACTGTTTGGCTGAAGGATGAAGTATCAATGAGTGCGCCAGTAA
AGGTTGATAATATTCCCATAAGTATTGCAATGTTTGCTTCTCTTTCATCGCAAACGAGTTACTTTGTATCTAAAATTGAT
GACTTTTTTGCTAAAAATGCTGAATTAGTCTTGTCTGAAGCGTTGAAGCTATATCAGGATGACAAAGATATCATAAAACT
AATTCATACAATCATTTACTCTGATAATAGACAATTTGTAAAAGCTTTTAGAAACACCGCTGTATCAGGCATTATAAGCG
AAAGCGCGCCTGAAACTTCTGCAGGAATTCAATCTACTCTTGGAAAGAATATTACTTCGCTACAATATTTAAAGCCTGGA
GGTAATTTTAGCATCAAGGAATGGTTTAGTAATTCTGCTGAAACTGGATGGTTATTTATCACAGCTAACCCAAGTCAAAG
AGCTACTTTACGCCCACTTATTTCAGCCTGGATAAGCATAGCTATCAAGGCTTTGATGTGTAGAAATCCTAATCATGATA
ACAAAAACATGTGGTTTATACTTGATGAACTTCCAGCTCTACAAAAAGTTTCGTCTTTACCAGTTGCTTTAGCTGAAAGT
AGAAAGTATGGAGGTTGCTTTGTTGCTGGATTGCAGAATATTCATCAATTAGAAGCAATATATGGTTCTGCTGAATGCGC
TTCTATGCTGGATTTGTTTAATAGTAAATTTATTTTTCGAGTTAGCGATCAGGTTACAGCTTATAAATCAGCATTAACAC
TAGGTGAGGAAGAAATTATTGAAACTCAAGAGAACTTGTCATATGGATCAAATACTATGAGAGATGGAGTAAATATGAAT
AATGTTGAGCGTAAAAAGCTTTTAGTTATGCCATCTGAAATTATGAATCTACCAGACCTTACATGTTATGTAAAGCTTGT
TGGTAACTTTCACATCACAAAACTAACTATGAAGCTACAAAACTTAAATATAGCATATGTTTGGGGATATACATTGCTCA
AAAAACTTAAGTTAGTGAATTATTAA

Upstream 100 bases:

>100_bases
GTGGTGGAGATCTGTTATGGCATGTGTTTAATGGCATAGGCAGAGGTCTTTGCTTCAAATAGCGAATACTTCACTCCAGT
GGGTCACTTAGCCTTGACTA

Downstream 100 bases:

>100_bases
TTCAAGAATTATCCATGCTAACTTTTAAATTAACCACTTCTTCGTTTGATAACCCAGTATTTTCAGCAATAAATTCAACT
GAAAAGCCAGCTTTTAATAA

Product: conjugative transfer protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MVAYGLLLGLFLEEISASLLWEWFFPSIFIFTLLFAPKSTVWLKDEVSMSAPVKVDNIPISIAMFASLSSQTSYFVSKID
DFFAKNAELVLSEALKLYQDDKDIIKLIHTIIYSDNRQFVKAFRNTAVSGIISESAPETSAGIQSTLGKNITSLQYLKPG
GNFSIKEWFSNSAETGWLFITANPSQRATLRPLISAWISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAES
RKYGGCFVAGLQNIHQLEAIYGSAECASMLDLFNSKFIFRVSDQVTAYKSALTLGEEEIIETQENLSYGSNTMRDGVNMN
NVERKKLLVMPSEIMNLPDLTCYVKLVGNFHITKLTMKLQNLNIAYVWGYTLLKKLKLVNY

Sequences:

>Translated_381_residues
MVAYGLLLGLFLEEISASLLWEWFFPSIFIFTLLFAPKSTVWLKDEVSMSAPVKVDNIPISIAMFASLSSQTSYFVSKID
DFFAKNAELVLSEALKLYQDDKDIIKLIHTIIYSDNRQFVKAFRNTAVSGIISESAPETSAGIQSTLGKNITSLQYLKPG
GNFSIKEWFSNSAETGWLFITANPSQRATLRPLISAWISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAES
RKYGGCFVAGLQNIHQLEAIYGSAECASMLDLFNSKFIFRVSDQVTAYKSALTLGEEEIIETQENLSYGSNTMRDGVNMN
NVERKKLLVMPSEIMNLPDLTCYVKLVGNFHITKLTMKLQNLNIAYVWGYTLLKKLKLVNY
>Mature_381_residues
MVAYGLLLGLFLEEISASLLWEWFFPSIFIFTLLFAPKSTVWLKDEVSMSAPVKVDNIPISIAMFASLSSQTSYFVSKID
DFFAKNAELVLSEALKLYQDDKDIIKLIHTIIYSDNRQFVKAFRNTAVSGIISESAPETSAGIQSTLGKNITSLQYLKPG
GNFSIKEWFSNSAETGWLFITANPSQRATLRPLISAWISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAES
RKYGGCFVAGLQNIHQLEAIYGSAECASMLDLFNSKFIFRVSDQVTAYKSALTLGEEEIIETQENLSYGSNTMRDGVNMN
NVERKKLLVMPSEIMNLPDLTCYVKLVGNFHITKLTMKLQNLNIAYVWGYTLLKKLKLVNY

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energize DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid IncFII R100 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 42806; Mature: 42806

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAYGLLLGLFLEEISASLLWEWFFPSIFIFTLLFAPKSTVWLKDEVSMSAPVKVDNIPI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEECCCCE
SIAMFASLSSQTSYFVSKIDDFFAKNAELVLSEALKLYQDDKDIIKLIHTIIYSDNRQFV
EHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHH
KAFRNTAVSGIISESAPETSAGIQSTLGKNITSLQYLKPGGNFSIKEWFSNSAETGWLFI
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCCCCEEEE
TANPSQRATLRPLISAWISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAES
EECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHCC
RKYGGCFVAGLQNIHQLEAIYGSAECASMLDLFNSKFIFRVSDQVTAYKSALTLGEEEII
CCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCHHHHH
ETQENLSYGSNTMRDGVNMNNVERKKLLVMPSEIMNLPDLTCYVKLVGNFHITKLTMKLQ
HHHHHCCCCCCHHHCCCCCCCCCHHEEEEECHHHCCCCCCEEEEEECCCEEEEEEEEEEE
NLNIAYVWGYTLLKKLKLVNY
CCCEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MVAYGLLLGLFLEEISASLLWEWFFPSIFIFTLLFAPKSTVWLKDEVSMSAPVKVDNIPI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEECCCCE
SIAMFASLSSQTSYFVSKIDDFFAKNAELVLSEALKLYQDDKDIIKLIHTIIYSDNRQFV
EHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHH
KAFRNTAVSGIISESAPETSAGIQSTLGKNITSLQYLKPGGNFSIKEWFSNSAETGWLFI
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCCCCEEEE
TANPSQRATLRPLISAWISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAES
EECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHCC
RKYGGCFVAGLQNIHQLEAIYGSAECASMLDLFNSKFIFRVSDQVTAYKSALTLGEEEII
CCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCHHHHH
ETQENLSYGSNTMRDGVNMNNVERKKLLVMPSEIMNLPDLTCYVKLVGNFHITKLTMKLQ
HHHHHCCCCCCHHHCCCCCCCCCHHEEEEECHHHCCCCCCEEEEEECCCEEEEEEEEEEE
NLNIAYVWGYTLLKKLKLVNY
CCCEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2680768; 7915817; 10464182; 3323526; 2164585; 2163400; 1618779; 10498725 [H]