Click here to switch to the map view.

The map label for this gene is fabI [H]

Identifier: 148284408

GI number: 148284408

Start: 681585

End: 682382

Strand: Direct

Name: fabI [H]

Synonym: OTBS_0706

Alternate gene names: 148284408

Gene position: 681585-682382 (Clockwise)

Preceding gene: 148284385

Following gene: 148284409

Centisome position: 32.04

GC content: 32.33

Gene sequence:

>798_bases
ATGCCAGGTAATAATATACAAAAAAATTTAGCCTTACTAACTGGCAAAAAGGGCGTTATACTTGGTGTAGCTAATTCAAT
GTCAATTGCTTGGAAAATTGCTGAACTATGCCATACTAATGAGGCTGAAATTGCTATAACATATCAAGATGATAAATTAA
AAACACGTATAGAGCCATTAGTTGAAGCAACAAAATGTAGTATGTTTCAGTGCGATGTTAAGAAAACAGCATCTATTGAT
AAATTATTTGATAATCTAGCAAATAATTGGGGTAAATTAGATTTTTTAGTACATTCAATCGCTTATACAGATAAAGAAGC
ACTTAAAGGACAATACATAAACACTTCTCAAGAGAACTTTCTTAATACGCTTGATATTTCTTGTTATTCTTTTACTTATT
TGAGCAAAAAAGCAGCCGAGCTAATGAAGAGTGGTGGAGACATACTGACTTTAAGTTACTATGGTGCTAAAAAGGTTATA
CCTTCATATAATGTTATGGGAGTCGCTAAAGCTGCATTAGAAGCTAGTGTAAGATATATAGCAGCTGATTTGGGAGCACA
AAATATCAGAGTAAATGCAATTTCATCTGGGCCAATTAGAACTTTATCAGCAAGTGCAATTAGCAATTTTAGTTTAATGC
TTAATATGCATCAGTCTTCTGCCCCACTAAAGCGAAATACTACTCAAAATGATGTTGCTAATGCTGCATTATATTTGCTA
AGTGATCTAAGTAATAGTATTACTGGGCAAATACACTATGTTGATTGTGGTTATAGTATTATGGGAATAGATAAGTAA

Upstream 100 bases:

>100_bases
ACTTTAAATCTTATGTTTACTACCATTGAGATATAATTAAAATGATAAAATACTAGAATTATCATTTAACATCAAGCAAA
TCTTAATTAGAATATGACAA

Downstream 100 bases:

>100_bases
CTAGCCTTCAGGATAACTCAAGATAGCAGCTATTCTTAGGCTAAACAGGCTACATTTACAATGTATTATAATAGTGTACT
ATTTAATAGATAATTTTTTG

Product: enoyl-[acyl carrier protein] reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MPGNNIQKNLALLTGKKGVILGVANSMSIAWKIAELCHTNEAEIAITYQDDKLKTRIEPLVEATKCSMFQCDVKKTASID
KLFDNLANNWGKLDFLVHSIAYTDKEALKGQYINTSQENFLNTLDISCYSFTYLSKKAAELMKSGGDILTLSYYGAKKVI
PSYNVMGVAKAALEASVRYIAADLGAQNIRVNAISSGPIRTLSASAISNFSLMLNMHQSSAPLKRNTTQNDVANAALYLL
SDLSNSITGQIHYVDCGYSIMGIDK

Sequences:

>Translated_265_residues
MPGNNIQKNLALLTGKKGVILGVANSMSIAWKIAELCHTNEAEIAITYQDDKLKTRIEPLVEATKCSMFQCDVKKTASID
KLFDNLANNWGKLDFLVHSIAYTDKEALKGQYINTSQENFLNTLDISCYSFTYLSKKAAELMKSGGDILTLSYYGAKKVI
PSYNVMGVAKAALEASVRYIAADLGAQNIRVNAISSGPIRTLSASAISNFSLMLNMHQSSAPLKRNTTQNDVANAALYLL
SDLSNSITGQIHYVDCGYSIMGIDK
>Mature_264_residues
PGNNIQKNLALLTGKKGVILGVANSMSIAWKIAELCHTNEAEIAITYQDDKLKTRIEPLVEATKCSMFQCDVKKTASIDK
LFDNLANNWGKLDFLVHSIAYTDKEALKGQYINTSQENFLNTLDISCYSFTYLSKKAAELMKSGGDILTLSYYGAKKVIP
SYNVMGVAKAALEASVRYIAADLGAQNIRVNAISSGPIRTLSASAISNFSLMLNMHQSSAPLKRNTTQNDVANAALYLLS
DLSNSITGQIHYVDCGYSIMGIDK

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI10190704, Length=260, Percent_Identity=26.1538461538462, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787545, Length=258, Percent_Identity=45.3488372093023, Blast_Score=237, Evalue=6e-64,
Organism=Escherichia coli, GI1787905, Length=190, Percent_Identity=30, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI2367365, Length=251, Percent_Identity=24.7011952191235, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17531453, Length=193, Percent_Identity=29.5336787564767, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=202, Percent_Identity=27.2277227722772, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28899; Mature: 28768

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGNNIQKNLALLTGKKGVILGVANSMSIAWKIAELCHTNEAEIAITYQDDKLKTRIEPL
CCCCCHHHCEEEEECCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHH
VEATKCSMFQCDVKKTASIDKLFDNLANNWGKLDFLVHSIAYTDKEALKGQYINTSQENF
HHHHHCCEEEECCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCCCCCHHHH
LNTLDISCYSFTYLSKKAAELMKSGGDILTLSYYGAKKVIPSYNVMGVAKAALEASVRYI
HHHCCCEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
AADLGAQNIRVNAISSGPIRTLSASAISNFSLMLNMHQSSAPLKRNTTQNDVANAALYLL
HHHCCCCEEEEEEECCCCCCEEEHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
SDLSNSITGQIHYVDCGYSIMGIDK
HHHCCCCCEEEEEEECCEEEEECCC
>Mature Secondary Structure 
PGNNIQKNLALLTGKKGVILGVANSMSIAWKIAELCHTNEAEIAITYQDDKLKTRIEPL
CCCCHHHCEEEEECCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHH
VEATKCSMFQCDVKKTASIDKLFDNLANNWGKLDFLVHSIAYTDKEALKGQYINTSQENF
HHHHHCCEEEECCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCCCCCHHHH
LNTLDISCYSFTYLSKKAAELMKSGGDILTLSYYGAKKVIPSYNVMGVAKAALEASVRYI
HHHCCCEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
AADLGAQNIRVNAISSGPIRTLSASAISNFSLMLNMHQSSAPLKRNTTQNDVANAALYLL
HHHCCCCEEEEEEECCCCCCEEEHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
SDLSNSITGQIHYVDCGYSIMGIDK
HHHCCCCCEEEEEEECCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA