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The map label for this gene is folC [H]

Identifier: 148284344

GI number: 148284344

Start: 556979

End: 558319

Strand: Direct

Name: folC [H]

Synonym: OTBS_0584

Alternate gene names: 148284344

Gene position: 556979-558319 (Clockwise)

Preceding gene: 148284337

Following gene: 148284346

Centisome position: 26.19

GC content: 31.69

Gene sequence:

>1341_bases
ATGGTTAAAATGCCGCATTGGCCAATTCCTTGGTGCAATCATATAAAACATGACCTAACTAGAATTAAATTATTACTATC
AGCTTTACAAAATCCGCATCTAAAAGTACCGCCAGTAATTCATGTTGCTGGAACTAATGGGAAAGGATCTACTAGTGCTT
ATTTAAGAGCAATATTTGAGACTGCTGGTTATAAAGTACATGGTTATACTTCTCCACATTTACTGCATTTTAATGAAAGG
ATTACAATTGCATCTGAGTATATTGATGATAGATATCTATTTAACATTATTGAAAAAACTAGAACTACAGCAGCTAAAGT
AAATATAACACCTACTTTTTTTGAAGGTACTACTGCCGCAGCATTTCTAGCATTTGCAGAAAATAAGGCAGATATTTTAC
TATTGGAAACTGGAATGGGCGGAAGACTAGATGCAACTAACGTAATAGATAATCCTATTGCAACAATAATTACCCCAATT
TCATATGATCACATGGAATACTTGGGCCCTACCCTAACTACTATTGCCTCTGAAAAAGCAGGCATTATTAAAGCTAATGC
CCAATGCATTATTAGTTGTCAAGCGCAACAAGTATATGATCTTCTAATAGATTATGCTATACGAGTAAAAGCTATACCTA
TAGTATATGAATATGACTTCGGTATTCAAAAACAAAATGATAGTTTCAAATTTTTATCTCATAATTTTACTTATCATTTT
TCTCTGCCATCTTTACCAGGTAACCACCAAATTGTTAATGCTGCCACTGCTATAGCAACATGTAAAGCTATAACTAAGCA
TTTTAATTTTAGTACTAATGATATTCAAAAAGGTATAAGTAATACTGTTTTGCCAGCAAGGTTAGAAAAAATTGATCTAT
TACAATATGATAAAATTTTTAACATAATTATGAAGCATTTATACAAAAGAAACATTGATGTACCACATAATATACCACAA
ATTTGGCTCGACGGTGCTCATAATACTGGCGGAGCTCAGTCATTATCTAATTTTATTAGAGAAAATCTAACACCACCAAT
ATATTTAATTCTAGGGATGACAAGCAACAGAGAAGTTAAACCTTTTTTATCATATTTTACAGATATTGTTACTCAAATTT
TTACAGTGAGTGTAATATCTGAACCTGCTAGCTATGATGCTGAAAAGCTAGCAGCATATGCTAACATTTCTGGGATCATA
TCGATTCCTACTGAATCATTAACAGATGCTATAATTTCAGCTATTAAATCAAATCAAGCTTTTGTACAACAAAATGGCAA
CATAATCATTACTGGCTCACTATTTTTAATGGCTGATTTTTATAATCTAATTAGAGGCTAA

Upstream 100 bases:

>100_bases
AAACTATTTACTAACAATCAAAATTGATAAGATTTGTATAATACAATCTAATAATACTAAAATTCCTCAAAATTAATTTT
ATTAAGAATATAATATTAAT

Downstream 100 bases:

>100_bases
TATAGATTATTTAGTATAAAATAAATACTCTATATAAAATAACCTCTATAAAAAAAAGAAAAACTAAAAAAAATTGCAAC
AAGTGTGTTTATAAAGCTCT

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MVKMPHWPIPWCNHIKHDLTRIKLLLSALQNPHLKVPPVIHVAGTNGKGSTSAYLRAIFETAGYKVHGYTSPHLLHFNER
ITIASEYIDDRYLFNIIEKTRTTAAKVNITPTFFEGTTAAAFLAFAENKADILLLETGMGGRLDATNVIDNPIATIITPI
SYDHMEYLGPTLTTIASEKAGIIKANAQCIISCQAQQVYDLLIDYAIRVKAIPIVYEYDFGIQKQNDSFKFLSHNFTYHF
SLPSLPGNHQIVNAATAIATCKAITKHFNFSTNDIQKGISNTVLPARLEKIDLLQYDKIFNIIMKHLYKRNIDVPHNIPQ
IWLDGAHNTGGAQSLSNFIRENLTPPIYLILGMTSNREVKPFLSYFTDIVTQIFTVSVISEPASYDAEKLAAYANISGII
SIPTESLTDAIISAIKSNQAFVQQNGNIIITGSLFLMADFYNLIRG

Sequences:

>Translated_446_residues
MVKMPHWPIPWCNHIKHDLTRIKLLLSALQNPHLKVPPVIHVAGTNGKGSTSAYLRAIFETAGYKVHGYTSPHLLHFNER
ITIASEYIDDRYLFNIIEKTRTTAAKVNITPTFFEGTTAAAFLAFAENKADILLLETGMGGRLDATNVIDNPIATIITPI
SYDHMEYLGPTLTTIASEKAGIIKANAQCIISCQAQQVYDLLIDYAIRVKAIPIVYEYDFGIQKQNDSFKFLSHNFTYHF
SLPSLPGNHQIVNAATAIATCKAITKHFNFSTNDIQKGISNTVLPARLEKIDLLQYDKIFNIIMKHLYKRNIDVPHNIPQ
IWLDGAHNTGGAQSLSNFIRENLTPPIYLILGMTSNREVKPFLSYFTDIVTQIFTVSVISEPASYDAEKLAAYANISGII
SIPTESLTDAIISAIKSNQAFVQQNGNIIITGSLFLMADFYNLIRG
>Mature_446_residues
MVKMPHWPIPWCNHIKHDLTRIKLLLSALQNPHLKVPPVIHVAGTNGKGSTSAYLRAIFETAGYKVHGYTSPHLLHFNER
ITIASEYIDDRYLFNIIEKTRTTAAKVNITPTFFEGTTAAAFLAFAENKADILLLETGMGGRLDATNVIDNPIATIITPI
SYDHMEYLGPTLTTIASEKAGIIKANAQCIISCQAQQVYDLLIDYAIRVKAIPIVYEYDFGIQKQNDSFKFLSHNFTYHF
SLPSLPGNHQIVNAATAIATCKAITKHFNFSTNDIQKGISNTVLPARLEKIDLLQYDKIFNIIMKHLYKRNIDVPHNIPQ
IWLDGAHNTGGAQSLSNFIRENLTPPIYLILGMTSNREVKPFLSYFTDIVTQIFTVSVISEPASYDAEKLAAYANISGII
SIPTESLTDAIISAIKSNQAFVQQNGNIIITGSLFLMADFYNLIRG

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=334, Percent_Identity=27.5449101796407, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI66932990, Length=334, Percent_Identity=27.5449101796407, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1788654, Length=435, Percent_Identity=26.4367816091954, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71984923, Length=444, Percent_Identity=24.7747747747748, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17553148, Length=444, Percent_Identity=24.7747747747748, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17553150, Length=444, Percent_Identity=24.7747747747748, Blast_Score=119, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6323760, Length=454, Percent_Identity=27.5330396475771, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6324815, Length=380, Percent_Identity=25.7894736842105, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24641571, Length=152, Percent_Identity=35.5263157894737, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24581568, Length=202, Percent_Identity=32.1782178217822, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 49664; Mature: 49664

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKMPHWPIPWCNHIKHDLTRIKLLLSALQNPHLKVPPVIHVAGTNGKGSTSAYLRAIFE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
TAGYKVHGYTSPHLLHFNERITIASEYIDDRYLFNIIEKTRTTAAKVNITPTFFEGTTAA
HCCEEEECCCCCEEEEECCEEEEHHHHHCHHHHHHHHHHHCCCEEEEEECEEEECCCHHH
AFLAFAENKADILLLETGMGGRLDATNVIDNPIATIITPISYDHMEYLGPTLTTIASEKA
HHHEEECCCCCEEEEECCCCCCCCCCHHHCCCHHHHCCCCCHHHHHHHCCHHHHHHCCCC
GIIKANAQCIISCQAQQVYDLLIDYAIRVKAIPIVYEYDFGIQKQNDSFKFLSHNFTYHF
CEEEECCEEEEEECHHHHHHHHHHHHHEEEEEEEEEEECCCEECCCCCEEEEECCEEEEE
SLPSLPGNHQIVNAATAIATCKAITKHFNFSTNDIQKGISNTVLPARLEKIDLLQYDKIF
ECCCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
NIIMKHLYKRNIDVPHNIPQIWLDGAHNTGGAQSLSNFIRENLTPPIYLILGMTSNREVK
HHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHH
PFLSYFTDIVTQIFTVSVISEPASYDAEKLAAYANISGIISIPTESLTDAIISAIKSNQA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCC
FVQQNGNIIITGSLFLMADFYNLIRG
EEECCCCEEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKMPHWPIPWCNHIKHDLTRIKLLLSALQNPHLKVPPVIHVAGTNGKGSTSAYLRAIFE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
TAGYKVHGYTSPHLLHFNERITIASEYIDDRYLFNIIEKTRTTAAKVNITPTFFEGTTAA
HCCEEEECCCCCEEEEECCEEEEHHHHHCHHHHHHHHHHHCCCEEEEEECEEEECCCHHH
AFLAFAENKADILLLETGMGGRLDATNVIDNPIATIITPISYDHMEYLGPTLTTIASEKA
HHHEEECCCCCEEEEECCCCCCCCCCHHHCCCHHHHCCCCCHHHHHHHCCHHHHHHCCCC
GIIKANAQCIISCQAQQVYDLLIDYAIRVKAIPIVYEYDFGIQKQNDSFKFLSHNFTYHF
CEEEECCEEEEEECHHHHHHHHHHHHHEEEEEEEEEEECCCEECCCCCEEEEECCEEEEE
SLPSLPGNHQIVNAATAIATCKAITKHFNFSTNDIQKGISNTVLPARLEKIDLLQYDKIF
ECCCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
NIIMKHLYKRNIDVPHNIPQIWLDGAHNTGGAQSLSNFIRENLTPPIYLILGMTSNREVK
HHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHH
PFLSYFTDIVTQIFTVSVISEPASYDAEKLAAYANISGIISIPTESLTDAIISAIKSNQA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCC
FVQQNGNIIITGSLFLMADFYNLIRG
EEECCCCEEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]