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The map label for this gene is phoB [H]

Identifier: 148284320

GI number: 148284320

Start: 520889

End: 521638

Strand: Direct

Name: phoB [H]

Synonym: OTBS_0551

Alternate gene names: 148284320

Gene position: 520889-521638 (Clockwise)

Preceding gene: 148284315

Following gene: 148284321

Centisome position: 24.49

GC content: 30.0

Gene sequence:

>750_bases
ATGTCTGCTTCTGAAAAGTTACCACCGCAAGTTTTGGTAATCGAGCTAGATGAGATACAACGAATTGCTATTTGTAATAT
CATAGAAAGGTCACTATTTAATGTTATTAGATTAAGCAATATTAATGATATCGAAACAAGCAGCATCAACGCAGTGCTTA
TATTTGCTCCAAATGTAATTATTGTTAGCACCTCTATCAAAGATGTTGTTCCAATTGATACATTTATTCAAAAGCTTAGA
AGTACCGCACAAAAATCTCATCGAATAGAAATACCTATAATTATTATTCTTAATCAAAACGATAATGTAGAAAATTATCA
AAAATTTGATAATGGTTTGATAGAAATTGTACAATATCCTTATACTGATTCTGAAATTATATTAGCAGTAAAGTCTTTGT
TACGCAGGTCTAGACCAATATTAAAAAACAGGCTGCTTAGATATAAAGACTTAACAATGGATCTGTCCAATTATAAAGTA
TATCGTGGTAATAAACCAATTCGTTTAGGTCCAACTGAATTTAAAATATTGCAGCTCTTATTAGAATCACCTAAAGTTAT
ACTTTCACGTGAGAAAATTATAGATTATGTATGGGGTCATGGACATAAAATTGAGCTTAGAACAGTTGATGTACATGTTA
ATAGATTAAGGAATGTTATCAAAAACAGTAAATTTGAACCAGCTTTAATTAAAACTGTTCGAGCTTCAGGATATTGTTTA
AAATTACCAGGAGAATCAGATTACGCTTGA

Upstream 100 bases:

>100_bases
GTATCTTTAAAAGTACTTTAAAAGTACTTGATTTTTTAATCAAGGGTTGTATTATAATGACCTTATTGCAATAATTGTTT
AATAATTTGGTATAATACTT

Downstream 100 bases:

>100_bases
CAATACTGTAGTATATATAAATACATACTTTATATTATTTTATATAAATAATATAAGTTATTCATAAGTGTATTTATTGT
AATGTAACATAATAGGAATC

Product: PhoB-like response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSASEKLPPQVLVIELDEIQRIAICNIIERSLFNVIRLSNINDIETSSINAVLIFAPNVIIVSTSIKDVVPIDTFIQKLR
STAQKSHRIEIPIIIILNQNDNVENYQKFDNGLIEIVQYPYTDSEIILAVKSLLRRSRPILKNRLLRYKDLTMDLSNYKV
YRGNKPIRLGPTEFKILQLLLESPKVILSREKIIDYVWGHGHKIELRTVDVHVNRLRNVIKNSKFEPALIKTVRASGYCL
KLPGESDYA

Sequences:

>Translated_249_residues
MSASEKLPPQVLVIELDEIQRIAICNIIERSLFNVIRLSNINDIETSSINAVLIFAPNVIIVSTSIKDVVPIDTFIQKLR
STAQKSHRIEIPIIIILNQNDNVENYQKFDNGLIEIVQYPYTDSEIILAVKSLLRRSRPILKNRLLRYKDLTMDLSNYKV
YRGNKPIRLGPTEFKILQLLLESPKVILSREKIIDYVWGHGHKIELRTVDVHVNRLRNVIKNSKFEPALIKTVRASGYCL
KLPGESDYA
>Mature_248_residues
SASEKLPPQVLVIELDEIQRIAICNIIERSLFNVIRLSNINDIETSSINAVLIFAPNVIIVSTSIKDVVPIDTFIQKLRS
TAQKSHRIEIPIIIILNQNDNVENYQKFDNGLIEIVQYPYTDSEIILAVKSLLRRSRPILKNRLLRYKDLTMDLSNYKVY
RGNKPIRLGPTEFKILQLLLESPKVILSREKIIDYVWGHGHKIELRTVDVHVNRLRNVIKNSKFEPALIKTVRASGYCLK
LPGESDYA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=25.3275109170306, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1786784, Length=186, Percent_Identity=31.1827956989247, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789809, Length=162, Percent_Identity=23.4567901234568, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788394, Length=153, Percent_Identity=26.1437908496732, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786911, Length=225, Percent_Identity=23.1111111111111, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790860, Length=186, Percent_Identity=24.1935483870968, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87082012, Length=165, Percent_Identity=29.0909090909091, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787229, Length=162, Percent_Identity=27.1604938271605, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 28600; Mature: 28469

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASEKLPPQVLVIELDEIQRIAICNIIERSLFNVIRLSNINDIETSSINAVLIFAPNVI
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEE
IVSTSIKDVVPIDTFIQKLRSTAQKSHRIEIPIIIILNQNDNVENYQKFDNGLIEIVQYP
EEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHHHHHCCHHEEEEECC
YTDSEIILAVKSLLRRSRPILKNRLLRYKDLTMDLSNYKVYRGNKPIRLGPTEFKILQLL
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCEEECCCEEEEECCCCEEECCHHHHHHHHH
LESPKVILSREKIIDYVWGHGHKIELRTVDVHVNRLRNVIKNSKFEPALIKTVRASGYCL
HHCCHHEEEHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHCCCEEE
KLPGESDYA
ECCCCCCCC
>Mature Secondary Structure 
SASEKLPPQVLVIELDEIQRIAICNIIERSLFNVIRLSNINDIETSSINAVLIFAPNVI
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEE
IVSTSIKDVVPIDTFIQKLRSTAQKSHRIEIPIIIILNQNDNVENYQKFDNGLIEIVQYP
EEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHHHHHCCHHEEEEECC
YTDSEIILAVKSLLRRSRPILKNRLLRYKDLTMDLSNYKVYRGNKPIRLGPTEFKILQLL
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCEEECCCEEEEECCCCEEECCHHHHHHHHH
LESPKVILSREKIIDYVWGHGHKIELRTVDVHVNRLRNVIKNSKFEPALIKTVRASGYCL
HHCCHHEEEHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHCCCEEE
KLPGESDYA
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]