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The map label for this gene is dnaN [H]

Identifier: 148283999

GI number: 148283999

Start: 1450

End: 2748

Strand: Direct

Name: dnaN [H]

Synonym: OTBS_0002

Alternate gene names: 148283999

Gene position: 1450-2748 (Clockwise)

Preceding gene: 148283998

Following gene: 148284000

Centisome position: 0.07

GC content: 28.41

Gene sequence:

>1299_bases
ATGAATACTAAGTATCCAAAGGTTGATCATAATGATCTAGTAATATCTGACTTTAACATCAAGCTTGATACTAAAAATTT
TTCCCGCGTTCTCAATTTTTGCATGCCTATACTGGAAAAAAAAAATGTAGATCAAATTTTAAGTAATGTTAAGCTTACTC
ATATCAATGATACTGTAGAAAAGCGTTTGGAAATAAGCGCTACTGATGGAGATATTTTTATTTACCAAAAGCTACCAATT
AAAATTAGTAAAAATGATATTAGCATAACTCTTCCATTACAGGCATTAAATGAAATAGTGAAAAAAATTCCACATGATGA
ATTTACTATCTATTGTAACCAAGATAATAAAATGGTGCAAATTAAAGGAGGTGAATATCTTGAGTTTAATCTAGCAACAT
TGCCTATCAATCTTTTTCCAGTAATTGAAGAACTTACTGATGTAGATTTTATTATTCACGTCACTGCTATGCAGTTAGCG
CAGATGATTGAATATACTAAATTTGCTATATCTACAGAAGAAACACGATATAATCTCAATGGTATTTATCTACATTCAAA
AGACTATAAATCATTAATAGCAGTAGCAACTGACGGGCACCGGCTTGCAAGCGCTAAATGCAACATTGATACTTTTAAAA
CCAATATATCTAATAATGATGGCTTTGGTGTTATTTTGCCACGTAAAACTATAACAGAATTATATAAAATTTTTAAAGAA
CCATCAATAAATCAATATAACGTCATTATAAAAGTAAAAAGTAATAAAATACAATTTGATTGTCAAAATATTACTATTAT
TTCAAAATTAATAGATGGTAACTTTCCAGAATATGAGCTATTTATTCCTGCTAATAACACAAAAAAAATAATTCTTAACT
CTAATGATCTTTCTGAATCAATTGATAGAGTTTCTACTATCGCAGTTGAAAAATCTAAAATAGTTATTTTAGATTTAGAT
CATCATGCTATTACAATTAGGGCTCACGGTAATGCTCAATGCAAGGCTAAAGAATTATTACTTCGACACCAGCATAATAA
CAAGAATGATCAACATCAATCTTCTAAACAGGAAGTAGAAAAATATGAATATATAGGTGATGATCAACTTACAATAGCAT
TTAATGCAAAATATTTACTAGATGTTTTAAGTGTATGTAATAATATTAAAATTCCGATTGAATTTAAAGACTCTATATCT
CCAGCTTTGATTCAATTCGAAGAAAAAAACACTAGTGCCACTTTTATAATAATGCCAGTTAGTGTCCCTACTCAAGAGTT
TGTTAGTACTCCTACTTAA

Upstream 100 bases:

>100_bases
TAAAACAGTAGAAGATCTAAGTAAACAGGAGCAAGAATTTGAAGATGAAGTACGTCTATTAATGAGAATACTGCAAAATT
AATATGAGTAATGAATAATT

Downstream 100 bases:

>100_bases
GAGTAAGATATTTATAATATAGTGTTTTTGCTATGCTATATATTTGCGTTGTTTATTTGTTGGTTATTTATGGTTTATTT
TTTAATAATGCTAATATTAG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MNTKYPKVDHNDLVISDFNIKLDTKNFSRVLNFCMPILEKKNVDQILSNVKLTHINDTVEKRLEISATDGDIFIYQKLPI
KISKNDISITLPLQALNEIVKKIPHDEFTIYCNQDNKMVQIKGGEYLEFNLATLPINLFPVIEELTDVDFIIHVTAMQLA
QMIEYTKFAISTEETRYNLNGIYLHSKDYKSLIAVATDGHRLASAKCNIDTFKTNISNNDGFGVILPRKTITELYKIFKE
PSINQYNVIIKVKSNKIQFDCQNITIISKLIDGNFPEYELFIPANNTKKIILNSNDLSESIDRVSTIAVEKSKIVILDLD
HHAITIRAHGNAQCKAKELLLRHQHNNKNDQHQSSKQEVEKYEYIGDDQLTIAFNAKYLLDVLSVCNNIKIPIEFKDSIS
PALIQFEEKNTSATFIIMPVSVPTQEFVSTPT

Sequences:

>Translated_432_residues
MNTKYPKVDHNDLVISDFNIKLDTKNFSRVLNFCMPILEKKNVDQILSNVKLTHINDTVEKRLEISATDGDIFIYQKLPI
KISKNDISITLPLQALNEIVKKIPHDEFTIYCNQDNKMVQIKGGEYLEFNLATLPINLFPVIEELTDVDFIIHVTAMQLA
QMIEYTKFAISTEETRYNLNGIYLHSKDYKSLIAVATDGHRLASAKCNIDTFKTNISNNDGFGVILPRKTITELYKIFKE
PSINQYNVIIKVKSNKIQFDCQNITIISKLIDGNFPEYELFIPANNTKKIILNSNDLSESIDRVSTIAVEKSKIVILDLD
HHAITIRAHGNAQCKAKELLLRHQHNNKNDQHQSSKQEVEKYEYIGDDQLTIAFNAKYLLDVLSVCNNIKIPIEFKDSIS
PALIQFEEKNTSATFIIMPVSVPTQEFVSTPT
>Mature_432_residues
MNTKYPKVDHNDLVISDFNIKLDTKNFSRVLNFCMPILEKKNVDQILSNVKLTHINDTVEKRLEISATDGDIFIYQKLPI
KISKNDISITLPLQALNEIVKKIPHDEFTIYCNQDNKMVQIKGGEYLEFNLATLPINLFPVIEELTDVDFIIHVTAMQLA
QMIEYTKFAISTEETRYNLNGIYLHSKDYKSLIAVATDGHRLASAKCNIDTFKTNISNNDGFGVILPRKTITELYKIFKE
PSINQYNVIIKVKSNKIQFDCQNITIISKLIDGNFPEYELFIPANNTKKIILNSNDLSESIDRVSTIAVEKSKIVILDLD
HHAITIRAHGNAQCKAKELLLRHQHNNKNDQHQSSKQEVEKYEYIGDDQLTIAFNAKYLLDVLSVCNNIKIPIEFKDSIS
PALIQFEEKNTSATFIIMPVSVPTQEFVSTPT

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=380, Percent_Identity=25.7894736842105, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 49300; Mature: 49300

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKYPKVDHNDLVISDFNIKLDTKNFSRVLNFCMPILEKKNVDQILSNVKLTHINDTVE
CCCCCCCCCCCCEEEEEEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEECHHHH
KRLEISATDGDIFIYQKLPIKISKNDISITLPLQALNEIVKKIPHDEFTIYCNQDNKMVQ
CCEEEEECCCCEEEEEEECEEEECCCEEEEEEHHHHHHHHHHCCCCCEEEEECCCCCEEE
IKGGEYLEFNLATLPINLFPVIEELTDVDFIIHVTAMQLAQMIEYTKFAISTEETRYNLN
EECCCEEEEEEEEECEEHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHEEECCCCEEEEE
GIYLHSKDYKSLIAVATDGHRLASAKCNIDTFKTNISNNDGFGVILPRKTITELYKIFKE
EEEEECCCCCEEEEEEECCCEEEEEECCCEEEEEECCCCCCEEEEECHHHHHHHHHHHCC
PSINQYNVIIKVKSNKIQFDCQNITIISKLIDGNFPEYELFIPANNTKKIILNSNDLSES
CCCCEEEEEEEEECCEEEEEECCEEEEEHHHCCCCCCEEEEEECCCCEEEEECCCCHHHH
IDRVSTIAVEKSKIVILDLDHHAITIRAHGNAQCKAKELLLRHQHNNKNDQHQSSKQEVE
HHHHHEEEEECCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
KYEYIGDDQLTIAFNAKYLLDVLSVCNNIKIPIEFKDSISPALIQFEEKNTSATFIIMPV
HHHCCCCCEEEEEECHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCEEEEEEEC
SVPTQEFVSTPT
CCCHHHHHCCCC
>Mature Secondary Structure
MNTKYPKVDHNDLVISDFNIKLDTKNFSRVLNFCMPILEKKNVDQILSNVKLTHINDTVE
CCCCCCCCCCCCEEEEEEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEECHHHH
KRLEISATDGDIFIYQKLPIKISKNDISITLPLQALNEIVKKIPHDEFTIYCNQDNKMVQ
CCEEEEECCCCEEEEEEECEEEECCCEEEEEEHHHHHHHHHHCCCCCEEEEECCCCCEEE
IKGGEYLEFNLATLPINLFPVIEELTDVDFIIHVTAMQLAQMIEYTKFAISTEETRYNLN
EECCCEEEEEEEEECEEHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHEEECCCCEEEEE
GIYLHSKDYKSLIAVATDGHRLASAKCNIDTFKTNISNNDGFGVILPRKTITELYKIFKE
EEEEECCCCCEEEEEEECCCEEEEEECCCEEEEEECCCCCCEEEEECHHHHHHHHHHHCC
PSINQYNVIIKVKSNKIQFDCQNITIISKLIDGNFPEYELFIPANNTKKIILNSNDLSES
CCCCEEEEEEEEECCEEEEEECCEEEEEHHHCCCCCCEEEEEECCCCEEEEECCCCHHHH
IDRVSTIAVEKSKIVILDLDHHAITIRAHGNAQCKAKELLLRHQHNNKNDQHQSSKQEVE
HHHHHEEEEECCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
KYEYIGDDQLTIAFNAKYLLDVLSVCNNIKIPIEFKDSISPALIQFEEKNTSATFIIMPV
HHHCCCCCEEEEEECHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCEEEEEEEC
SVPTQEFVSTPT
CCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]