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The map label for this gene is ldh2

Identifier: 148269033

GI number: 148269033

Start: 2774640

End: 2775599

Strand: Reverse

Name: ldh2

Synonym: SaurJH9_2624

Alternate gene names: 148269033

Gene position: 2775599-2774640 (Counterclockwise)

Preceding gene: 148269035

Following gene: 148269032

Centisome position: 95.49

GC content: 35.42

Gene sequence:

>960_bases
ATGAAAACATTTGGTAAAAAGGTTGTATTAATCGGAGATGGATCTGTAGGATCAAGCTATGCCTTTGCAATGGTTACGCA
AGGTGTTGCTGATGAATTTGTAATTATTGACATTGCAAAAGACAAAGTAAAAGCAGATGTTCAAGATTTAAACCATGGTA
CAGTCCACAGTCCTTCACCAGTTGATGTGAAAGCAGGTGAATACGAAGACTGTAAAGATGCAGATTTAGTTGTTATTACA
GCTGGTGCACCTCAAAAGCCAGGTGAAACACGTTTACAATTAGTTGAAAAAAATACTAAGATTATGAAGAGCATTGTTAA
GAGTGTTATGGATAGCGGCTTTGATGGATATTTCTTAATCGCGGCAAACCCTGTTGACATTTTAACAAGATTTGTAAAAG
AATATACTGGTTTACCAGCAGAGCGTGTTATCGGTTCAGGTACTGTATTGGACAGTGCACGTTTACAATATTTAATTAGC
CAAGAACTTGGTGTTGCACCTTCAAGTGTTGACGCTAGTATTATTGGTGAGCATGGTGATACTGAACTTGCAGTTTGGTC
ACAAGCAAATGTAGCAGGTATTTCAGTATATGACACATTAAAAGAACAAACTGGTAGCGAAGCTAAAGCGGAAGAAATTT
ATGTAAATACACGTGACGCTGCTTATGAAATTATCCAAGCTAAAGGGTCAACATACTATGGAATTGCATTAGCATTGATG
CGCATTTCAAAAGCCATTTTAAATAATGAAAATAATGTCTTAAATGTTTCTATACAATTAGATGGTCAATATGGTGGTCA
CAAAGGCGTTTACCTAGGTGTACCAACATTAGTTAACCAACATGGCGCAGTTAAAATTTATGAAATGCCATTAAGTGCCG
AAGAACAAGCGTTGTTCGATAAATCTGTTAAAATATTAGAAGATACATTTGATTCAATTAAATATTTATTAGAAGACTAA

Upstream 100 bases:

>100_bases
TATTATAATAAAATAAAATTGTTAATCTTTAATTTCAGTATAGATATTTTTACGTGTAGTCACGTGTAAAATAAATTCAA
TTAGGTTAGGAGACATAATT

Downstream 100 bases:

>100_bases
GCCTATTTTAAGTATTAATTAGAAATATATTAATGGTAAGAGGATCTATTAGTGTTGCAGTAACATGTGGCACTGATAGA
TCCATTTTTTTAGCAAGCAT

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 2

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVKADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVIT
AGAPQKPGETRLQLVEKNTKIMKSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTRDAAYEIIQAKGSTYYGIALALM
RISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPTLVNQHGAVKIYEMPLSAEEQALFDKSVKILEDTFDSIKYLLED

Sequences:

>Translated_319_residues
MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVKADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVIT
AGAPQKPGETRLQLVEKNTKIMKSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTRDAAYEIIQAKGSTYYGIALALM
RISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPTLVNQHGAVKIYEMPLSAEEQALFDKSVKILEDTFDSIKYLLED
>Mature_319_residues
MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVKADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVIT
AGAPQKPGETRLQLVEKNTKIMKSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTRDAAYEIIQAKGSTYYGIALALM
RISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPTLVNQHGAVKIYEMPLSAEEQALFDKSVKILEDTFDSIKYLLED

Specific function: Contributes to S.aureus growth during nitrosative stress in both aerobically and anaerobically cultured cells, despite playing a secondary role in this resistance mechanism

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=39.344262295082, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=39.344262295082, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI5031857, Length=308, Percent_Identity=37.987012987013, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI260099723, Length=308, Percent_Identity=37.987012987013, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI291575128, Length=313, Percent_Identity=36.1022364217252, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI4557032, Length=313, Percent_Identity=36.1022364217252, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI15082234, Length=311, Percent_Identity=36.3344051446945, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI47059044, Length=311, Percent_Identity=36.3344051446945, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI221136809, Length=311, Percent_Identity=36.3344051446945, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI260099725, Length=229, Percent_Identity=38.8646288209607, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI260099727, Length=211, Percent_Identity=40.7582938388626, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI207028494, Length=308, Percent_Identity=30.5194805194805, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI103472011, Length=287, Percent_Identity=25.0871080139373, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI103472015, Length=183, Percent_Identity=28.4153005464481, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1789632, Length=326, Percent_Identity=27.3006134969325, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17535107, Length=296, Percent_Identity=38.8513513513513, Blast_Score=218, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI17554310, Length=252, Percent_Identity=29.3650793650794, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322765, Length=230, Percent_Identity=26.5217391304348, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17136226, Length=318, Percent_Identity=37.1069182389937, Blast_Score=231, Evalue=4e-61,
Organism=Drosophila melanogaster, GI45550422, Length=313, Percent_Identity=30.0319488817891, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24647881, Length=315, Percent_Identity=27.6190476190476, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH2_STAA1 (A7X6Y1)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001443176.1
- ProteinModelPortal:   A7X6Y1
- SMR:   A7X6Y1
- STRING:   A7X6Y1
- EnsemblBacteria:   EBSTAT00000002835
- GeneID:   5560161
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2586
- eggNOG:   COG0039
- GeneTree:   EBGT00050000024431
- HOGENOM:   HBG566126
- OMA:   ISGFPKH
- ProtClustDB:   CLSK886101
- BioCyc:   SAUR418127:SAHV_2586-MONOMER
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 34432; Mature: 34432

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00064 L_LDH

Important sites: ACT_SITE 178-178 BINDING 91-91 BINDING 123-123 BINDING 154-154 BINDING 231-231

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVKADVQDLNHGTVHSPSP
CCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCC
VDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIMKSIVKSVMDSGFDGYFLI
CCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLISQELGVAPSSVDASIIGEHGD
ECCHHHHHHHHHHHHHCCCHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
TELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTRDAAYEIIQAKGSTYYGIALALM
CEEEEEECCCEEEEHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHH
RISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPTLVNQHGAVKIYEMPLSAEEQALFD
HHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEECHHHHCCCCCEEEEECCCCCCHHHHHH
KSVKILEDTFDSIKYLLED
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVKADVQDLNHGTVHSPSP
CCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCC
VDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIMKSIVKSVMDSGFDGYFLI
CCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLISQELGVAPSSVDASIIGEHGD
ECCHHHHHHHHHHHHHCCCHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
TELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTRDAAYEIIQAKGSTYYGIALALM
CEEEEEECCCEEEEHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHH
RISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPTLVNQHGAVKIYEMPLSAEEQALFD
HHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEECHHHHCCCCCEEEEECCCCCCHHHHHH
KSVKILEDTFDSIKYLLED
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA