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The map label for this gene is mtnE [H]

Identifier: 148268992

GI number: 148268992

Start: 2735280

End: 2736434

Strand: Reverse

Name: mtnE [H]

Synonym: SaurJH9_2582

Alternate gene names: 148268992

Gene position: 2736434-2735280 (Counterclockwise)

Preceding gene: 148268993

Following gene: 148268991

Centisome position: 94.14

GC content: 36.36

Gene sequence:

>1155_bases
ATGATCTCTAACAAATTAGCAAATATCCCTGATAGTTATTTCGGGAAAACGATGGGACGTAAAATCGAACATGGACCATT
GCCATTAATTAATATGGCAGTTGGGATCCCTGATGGGCCCACGCCACAAGGTATTATTGATCATTTTCAAAAAGCACTAA
CTATTCCTGAAAATCAAAAATATGGTGCTTTTCATGGTAAAGAAGCGTTCAAGCAAGCTATTGTAGATTTTTATCAAAGA
CAATATAATGTGACGTTAGATAAAGAAGATGAAGTATGCATTTTATATGGTACAAAAAATGGATTAGTGGCAGTACCAAC
ATGTGTTATCAATCCAGGAGACTATGTATTACTACCTGATCCAGGCTACACGGACTATTTAGCAGGTGTACTTTTAGCTG
ATGGCAAGCCAGTTCCGCTTAATTTAGAACCGCCACATTATTTGCCAGATTGGTCCACAGTTGATTCACAAATAATAGAT
AAAACAAAACTCATTTATTTAACGTATCCAAATAATCCAACTGGATCGACAGCTACGAAAGAAGTTTTTGATGAAGCGAT
AGCTAAATTTAAAGGTACAGACACTAAAATCGTGCATGATTTTGCTTATGGTGCTTTCGGTTTCGACGCTAAAAATCCTA
GTATACTTGCTTCGGAAAATGGGAAAGATGTTGCGATTGAAATTTATTCCTTGTCTAAAGGTTATAACATGTCAGGTTTT
AGAGTCGGTTTTGCAGTTGGTAATAAAGATATGATTCAAGCTTTGAAAAAGTACCAAACGCACACAAATGCTGGGATGTT
TGGTGCATTACAAGATGCCGCAATATATGCTTTGAATCATTATGATGATTTTTTAGAAGAACAAAGCAATGTATTTAAAA
CACGTCGTGACCGATTCGAAGCCATGCTTGCAAAAGCTGATTTGCCATTTGTTCATGCTAAAGGTGGTATATATGTATGG
TTGGAAACACCGCCTGGTTATGACAGCGAGCAATTTGAGCAATTTTTAGTGCAAGAGAAGTCAATACTTGTTGCTCCAGG
GAAACCATTTGGAGAGAATGGTAATCGTTATGTGAGAATTTCATTGGCGTTAGATGACCAGAAATTAGACGAAGCTGCAA
TAAGATTAACAGAACTAGCATATTTATATGAATAA

Upstream 100 bases:

>100_bases
TGAATGGCGATGTCATTGTAACCATCTAAATGAGAGTGATACAAGTTGTTAGGATTATGATAGTCGCCCAGGCTATGTTT
AATGATAGAGGAGGAATTTG

Downstream 100 bases:

>100_bases
TAAAGTTAAGGGTGATGTTAAAATGACAAAAATTAAAATCATGAGTGTACGTGATGAAGATATGCCTTATATCAAGGCGT
GGGCAGAGAAACATCATGTT

Product: aminotransferase, class I and II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQGIIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQR
QYNVTLDKEDEVCILYGTKNGLVAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSTVDSQIID
KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILASENGKDVAIEIYSLSKGYNMSGF
RVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIYALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVW
LETPPGYDSEQFEQFLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE

Sequences:

>Translated_384_residues
MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQGIIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQR
QYNVTLDKEDEVCILYGTKNGLVAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSTVDSQIID
KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILASENGKDVAIEIYSLSKGYNMSGF
RVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIYALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVW
LETPPGYDSEQFEQFLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE
>Mature_384_residues
MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQGIIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQR
QYNVTLDKEDEVCILYGTKNGLVAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSTVDSQIID
KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILASENGKDVAIEIYSLSKGYNMSGF
RVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIYALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVW
LETPPGYDSEQFEQFLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE

Specific function: Catalyzes the formation of methionine from 2-keto-4- methylthiobutyrate (KMTB) (Probable) [H]

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. MtnE subfamily [H]

Homologues:

Organism=Homo sapiens, GI56713256, Length=378, Percent_Identity=23.5449735449735, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI56713254, Length=381, Percent_Identity=23.8845144356955, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI95147551, Length=274, Percent_Identity=22.6277372262774, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI169881279, Length=274, Percent_Identity=22.6277372262774, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1788722, Length=350, Percent_Identity=32.5714285714286, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI1786816, Length=381, Percent_Identity=22.8346456692913, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1788627, Length=368, Percent_Identity=25.5434782608696, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787710, Length=325, Percent_Identity=25.2307692307692, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994472, Length=390, Percent_Identity=25.3846153846154, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71994476, Length=390, Percent_Identity=25.3846153846154, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17567663, Length=337, Percent_Identity=27.893175074184, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17567369, Length=395, Percent_Identity=22.2784810126582, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322401, Length=391, Percent_Identity=22.7621483375959, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI18859735, Length=406, Percent_Identity=25.615763546798, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28573069, Length=414, Percent_Identity=20.2898550724638, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24646114, Length=414, Percent_Identity=20.2898550724638, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI28573067, Length=414, Percent_Identity=20.2898550724638, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI28573065, Length=414, Percent_Identity=20.2898550724638, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.6.1.- [C]

Molecular weight: Translated: 42708; Mature: 42708

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50821 PAZ ; PS00105 AA_TRANSFER_CLASS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQGIIDHFQKALTIPENQK
CCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
YGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGLVAVPTCVINPGDYVLLPD
CCCCCCHHHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCCEEEEEEEEECCCCEEEECC
PGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSTVDSQIIDKTKLIYLTYPNNPTGSTATK
CCCHHHHHHEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEEEECCCCCCCCHHHH
EVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILASENGKDVAIEIYSLSKGYNMSGF
HHHHHHHHHHCCCCCEEEEEHHCCEECCCCCCCCEEECCCCCEEEEEEEEECCCCCCCCE
RVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIYALNHYDDFLEEQSNVFKTRRDRFE
EEEEEECCHHHHHHHHHHHHCCCCCCCCCHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHH
AMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQFLVQEKSILVAPGKPFGENGNRYVRI
HHHHHCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEE
SLALDDQKLDEAAIRLTELAYLYE
EEEECCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQGIIDHFQKALTIPENQK
CCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
YGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGLVAVPTCVINPGDYVLLPD
CCCCCCHHHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCCEEEEEEEEECCCCEEEECC
PGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSTVDSQIIDKTKLIYLTYPNNPTGSTATK
CCCHHHHHHEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEEEECCCCCCCCHHHH
EVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILASENGKDVAIEIYSLSKGYNMSGF
HHHHHHHHHHCCCCCEEEEEHHCCEECCCCCCCCEEECCCCCEEEEEEEEECCCCCCCCE
RVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIYALNHYDDFLEEQSNVFKTRRDRFE
EEEEEECCHHHHHHHHHHHHCCCCCCCCCHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHH
AMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQFLVQEKSILVAPGKPFGENGNRYVRI
HHHHHCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEE
SLALDDQKLDEAAIRLTELAYLYE
EEEECCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921 [H]