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The map label for this gene is speE1 [H]

Identifier: 148261181

GI number: 148261181

Start: 2421470

End: 2422333

Strand: Reverse

Name: speE1 [H]

Synonym: Acry_2191

Alternate gene names: 148261181

Gene position: 2422333-2421470 (Counterclockwise)

Preceding gene: 148261182

Following gene: 148261176

Centisome position: 71.47

GC content: 66.78

Gene sequence:

>864_bases
ATGACCGACATGTGGGTGAACGAGACGCTGTATCCGTTCTGGGGCCAGCGCTTCGGGGTGACGCGCGAGCTGGCGCGGAC
GAAGAGCGACTTCCAGGACATCGCGATCTTCGAGAGCGAGTTCCACGGACGGGTGATGATGCTGGACGGCATCGTGCAGA
TCACCGAGGGCGACGAGTTCGTCTACCAGGAGATGCTGACGCATGTGCCGCTGCTGGCGCATGGGGCGGCGCGATCGGTG
CTGATCATCGGCGCCGGCGATGGCGGCGTGCTGCGGCGGGTGCTGATGCACAAGGGCGTCGAGCGGGCGGTGATGGTCGA
GATCGACGGCGAGGTGATCCGGCTGGCGAAGGAGTTCCTGCCGAAGATCGCGGGCGAGGCCTGGGACGATCCGCGCGCGG
AGGTGATCGTCGGCGACGGGATCGACTATGTGAAGAAGGCGCCGGATGCGAGTTTCGACGTCATCATCGTCGATTCGACC
GATCCGATCGGGGTGGGCGAGGTGCTGTTCACCGATGATTTCTATGCCAATTGCGCGCGCATCCTGACCGCGCGGGGGCT
GGTGGTGAACCAGTGCGGCGTGCCGTTCATGCAGGCGGACGAGCTGCGCGAGACCTCGGCGCGGCGGCGGAAATTCTTTC
CGCACGTGACCGCCTATGTCGCCGCGGTGCCGACCTATGTCGGCGGGTTCATGACGCTGGGCTGGGCCGGCAAGGACCCC
GAACTCACCCGTCTGCCGGCGGCGACGATCCGCGCGCGGGCGGAAGCGGCGGGGATTCTCGGGACCACGGAATACTGGTC
GCCGGAGATGCACGCGGCGAGCTTCGTGCTGCCGCCCTATATCGCGAAGCACCTGCCGGGCTGA

Upstream 100 bases:

>100_bases
GGGCGAACAGCGCCGCGGCGTCACCGCCTGAGGCGAAACCGTTGATGACGACACACCGCCTTGCCACCGGCAGGGCGGTG
TTTTCGCATGGAGCGAAGAC

Downstream 100 bases:

>100_bases
GCTTCGGCGCCCGGTCCCGTTCGGGGTCGGGCGCGAGGCCGGGGTTCAGCCGTCCTGGCCGCGCTGCTCGCTGTGGGTGC
GGTGCTGCACGCGGCCGATC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 1; PAPT 1; SPDSY 1 [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTDMWVNETLYPFWGQRFGVTRELARTKSDFQDIAIFESEFHGRVMMLDGIVQITEGDEFVYQEMLTHVPLLAHGAARSV
LIIGAGDGGVLRRVLMHKGVERAVMVEIDGEVIRLAKEFLPKIAGEAWDDPRAEVIVGDGIDYVKKAPDASFDVIIVDST
DPIGVGEVLFTDDFYANCARILTARGLVVNQCGVPFMQADELRETSARRRKFFPHVTAYVAAVPTYVGGFMTLGWAGKDP
ELTRLPAATIRARAEAAGILGTTEYWSPEMHAASFVLPPYIAKHLPG

Sequences:

>Translated_287_residues
MTDMWVNETLYPFWGQRFGVTRELARTKSDFQDIAIFESEFHGRVMMLDGIVQITEGDEFVYQEMLTHVPLLAHGAARSV
LIIGAGDGGVLRRVLMHKGVERAVMVEIDGEVIRLAKEFLPKIAGEAWDDPRAEVIVGDGIDYVKKAPDASFDVIIVDST
DPIGVGEVLFTDDFYANCARILTARGLVVNQCGVPFMQADELRETSARRRKFFPHVTAYVAAVPTYVGGFMTLGWAGKDP
ELTRLPAATIRARAEAAGILGTTEYWSPEMHAASFVLPPYIAKHLPG
>Mature_286_residues
TDMWVNETLYPFWGQRFGVTRELARTKSDFQDIAIFESEFHGRVMMLDGIVQITEGDEFVYQEMLTHVPLLAHGAARSVL
IIGAGDGGVLRRVLMHKGVERAVMVEIDGEVIRLAKEFLPKIAGEAWDDPRAEVIVGDGIDYVKKAPDASFDVIIVDSTD
PIGVGEVLFTDDFYANCARILTARGLVVNQCGVPFMQADELRETSARRRKFFPHVTAYVAAVPTYVGGFMTLGWAGKDPE
LTRLPAATIRARAEAAGILGTTEYWSPEMHAASFVLPPYIAKHLPG

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=283, Percent_Identity=36.7491166077738, Blast_Score=187, Evalue=1e-47,
Organism=Escherichia coli, GI1786312, Length=279, Percent_Identity=46.9534050179211, Blast_Score=263, Evalue=9e-72,
Organism=Caenorhabditis elegans, GI25154106, Length=274, Percent_Identity=40.1459854014599, Blast_Score=168, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI212645616, Length=227, Percent_Identity=42.2907488986784, Blast_Score=160, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6323175, Length=294, Percent_Identity=37.4149659863946, Blast_Score=192, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6325326, Length=289, Percent_Identity=34.9480968858132, Blast_Score=184, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24645443, Length=290, Percent_Identity=37.5862068965517, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI21355941, Length=223, Percent_Identity=35.4260089686099, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI21358269, Length=150, Percent_Identity=30, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24663238, Length=149, Percent_Identity=30.2013422818792, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 31690; Mature: 31559

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDMWVNETLYPFWGQRFGVTRELARTKSDFQDIAIFESEFHGRVMMLDGIVQITEGDEF
CCCCCCCCCCCCHHCCHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECEEEEECCCHH
VYQEMLTHVPLLAHGAARSVLIIGAGDGGVLRRVLMHKGVERAVMVEIDGEVIRLAKEFL
HHHHHHHHCCHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHH
PKIAGEAWDDPRAEVIVGDGIDYVKKAPDASFDVIIVDSTDPIGVGEVLFTDDFYANCAR
HHHCCCCCCCCCCEEEEECCHHHHHCCCCCCEEEEEEECCCCCCCCCEEECCHHHHHHHH
ILTARGLVVNQCGVPFMQADELRETSARRRKFFPHVTAYVAAVPTYVGGFMTLGWAGKDP
HHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCCCCCC
ELTRLPAATIRARAEAAGILGTTEYWSPEMHAASFVLPPYIAKHLPG
CCHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCHHHHHCCCC
>Mature Secondary Structure 
TDMWVNETLYPFWGQRFGVTRELARTKSDFQDIAIFESEFHGRVMMLDGIVQITEGDEF
CCCCCCCCCCCHHCCHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECEEEEECCCHH
VYQEMLTHVPLLAHGAARSVLIIGAGDGGVLRRVLMHKGVERAVMVEIDGEVIRLAKEFL
HHHHHHHHCCHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHH
PKIAGEAWDDPRAEVIVGDGIDYVKKAPDASFDVIIVDSTDPIGVGEVLFTDDFYANCAR
HHHCCCCCCCCCCEEEEECCHHHHHCCCCCCEEEEEEECCCCCCCCCEEECCHHHHHHHH
ILTARGLVVNQCGVPFMQADELRETSARRRKFFPHVTAYVAAVPTYVGGFMTLGWAGKDP
HHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCCCCCC
ELTRLPAATIRARAEAAGILGTTEYWSPEMHAASFVLPPYIAKHLPG
CCHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]