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The map label for this gene is proP [H]

Identifier: 148261167

GI number: 148261167

Start: 2401884

End: 2403209

Strand: Reverse

Name: proP [H]

Synonym: Acry_2177

Alternate gene names: 148261167

Gene position: 2403209-2401884 (Counterclockwise)

Preceding gene: 148261168

Following gene: 148261166

Centisome position: 70.91

GC content: 67.95

Gene sequence:

>1326_bases
ATGCCGCACCCTGCTTCTGAGCCGCACCCGGCTTCCTCCGCCACCGCGCCCTCGCGGCGCAGGAAACGCCGCACCCTCTC
GCTCGGCAATCTCAGCGCCGGCACGATCGGGAACGTGCTGGAATGGTACGATTTCGGCCTCTACGGCTATCTCGCGCCGA
TGATCGCCAGCCTGTTCTTCCCCTCGTCCGACAAGATCGCCTCGTTGCTCGGCGCCTATGGCGGGTTCGCCATCGGGTTC
ATGATGCGGCCGATCGGCGGGGTGATCTTCGGCCATCTCGGCGACCGGGTGGGGCGGCAGACGGTGCTGGTGACCTCGGT
GGTGATGATGGGGCTGGCGACCACGGCGGTCGGGCTGTTGCCGACCTATCAGCAGATCGGCTTCTGGGCGCCGGTGCTGC
TGCTGGTGGTGCGGCTGTTCCAGGGCCTGTCGGTGGGCGGGGAGTTCACCGGCTCGGTGTCGTATCTCGTGGAAACCGCG
CCGGCGAACCGGCGGGGCTTCGCCGGCAGTTTCGCGAATTTCGGCTCGACCGGCGGCTATCTGCTCGCCGCCGGCTTCGC
CGCGCTGACCACGCTGATGGCGGGCCATCATGCGGTGCAGGGCTGGGCCTGGCGCGTGCCGTTCCTCGCCGGCGGCGTGC
TCGCGGTGCTGGCCTATCTGGTGCGTTCGCGGCTGACCGATACCGGATATGAGCCCGACAAGGCGGCGGAGGATGACGAA
CTGCCGATCCGCCAGGCGTTCCGCCGCAGCCCGAAGATCATGCTGCTGGCGATGCTGTTCACCTGGGGCTATGGCGTCGC
GGATTATCTCACCCTCGTGTTCCTGCCGACCTTCGGGTCCAAGTTCGGCGCGATTTCCGACGGCCTGGCGCTGGAGATCG
TGACCGTCACGCAGCTTTTCGTGCTTGTCCTGATCCCGCTGACCGGCTGGCTGACCGATCACTGGATCCTGCGCCGCTCC
TGGCTGATCGGCAATTTCGGCTTGCTGCTGGTTCTGGCGCTGCCCTTCTTCGCGATGGCGCAGGGGGCGTTGACGCCCTT
CGCCATCGCCCAGGTGGTCTCGGGCCTGATGCTCGGCTCGATCATGGGCGTCGCCCCGGCGATGCTCTCCGAGCAGTTCC
GCTCTGAATACCGGCTGTCGGGCTATTCGCTGGCGTTCAATGTCGGGCTCGGGATCGGCGGCGGCACGGCGCCGCTGATC
GCCACCGCGCTGATCGGGCTGACCGGAGTGAAGCTCGCCGCCGGCGCCTATCTGCTGGTCGGCTGCGCGATGTCGGTGGT
CGCGCTGTGGATGCTGAAGGACCGCGGCCGCCAGCCCCTGAGGTGA

Upstream 100 bases:

>100_bases
GGCCGCAACCCAGGACCCGACCGGTTCGCCCCGCGCCGCCCCTCACCGGGGCGCGGCGATGCGTCGCCGCCGTTCGGGAA
GAACGAAAGCGAGGTCCAAG

Downstream 100 bases:

>100_bases
GCGCCGTGCGGCGGCGGGGCGATTGACGCGCCCGCCGCGATCGGACAGGTTTCGGGCGAGGAATGTCATGAAACCTGCTC
CATTCGATTACGTCCGTGCC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MPHPASEPHPASSATAPSRRRKRRTLSLGNLSAGTIGNVLEWYDFGLYGYLAPMIASLFFPSSDKIASLLGAYGGFAIGF
MMRPIGGVIFGHLGDRVGRQTVLVTSVVMMGLATTAVGLLPTYQQIGFWAPVLLLVVRLFQGLSVGGEFTGSVSYLVETA
PANRRGFAGSFANFGSTGGYLLAAGFAALTTLMAGHHAVQGWAWRVPFLAGGVLAVLAYLVRSRLTDTGYEPDKAAEDDE
LPIRQAFRRSPKIMLLAMLFTWGYGVADYLTLVFLPTFGSKFGAISDGLALEIVTVTQLFVLVLIPLTGWLTDHWILRRS
WLIGNFGLLLVLALPFFAMAQGALTPFAIAQVVSGLMLGSIMGVAPAMLSEQFRSEYRLSGYSLAFNVGLGIGGGTAPLI
ATALIGLTGVKLAAGAYLLVGCAMSVVALWMLKDRGRQPLR

Sequences:

>Translated_441_residues
MPHPASEPHPASSATAPSRRRKRRTLSLGNLSAGTIGNVLEWYDFGLYGYLAPMIASLFFPSSDKIASLLGAYGGFAIGF
MMRPIGGVIFGHLGDRVGRQTVLVTSVVMMGLATTAVGLLPTYQQIGFWAPVLLLVVRLFQGLSVGGEFTGSVSYLVETA
PANRRGFAGSFANFGSTGGYLLAAGFAALTTLMAGHHAVQGWAWRVPFLAGGVLAVLAYLVRSRLTDTGYEPDKAAEDDE
LPIRQAFRRSPKIMLLAMLFTWGYGVADYLTLVFLPTFGSKFGAISDGLALEIVTVTQLFVLVLIPLTGWLTDHWILRRS
WLIGNFGLLLVLALPFFAMAQGALTPFAIAQVVSGLMLGSIMGVAPAMLSEQFRSEYRLSGYSLAFNVGLGIGGGTAPLI
ATALIGLTGVKLAAGAYLLVGCAMSVVALWMLKDRGRQPLR
>Mature_440_residues
PHPASEPHPASSATAPSRRRKRRTLSLGNLSAGTIGNVLEWYDFGLYGYLAPMIASLFFPSSDKIASLLGAYGGFAIGFM
MRPIGGVIFGHLGDRVGRQTVLVTSVVMMGLATTAVGLLPTYQQIGFWAPVLLLVVRLFQGLSVGGEFTGSVSYLVETAP
ANRRGFAGSFANFGSTGGYLLAAGFAALTTLMAGHHAVQGWAWRVPFLAGGVLAVLAYLVRSRLTDTGYEPDKAAEDDEL
PIRQAFRRSPKIMLLAMLFTWGYGVADYLTLVFLPTFGSKFGAISDGLALEIVTVTQLFVLVLIPLTGWLTDHWILRRSW
LIGNFGLLLVLALPFFAMAQGALTPFAIAQVVSGLMLGSIMGVAPAMLSEQFRSEYRLSGYSLAFNVGLGIGGGTAPLIA
TALIGLTGVKLAAGAYLLVGCAMSVVALWMLKDRGRQPLR

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=451, Percent_Identity=29.490022172949, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI1788292, Length=444, Percent_Identity=34.9099099099099, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1788942, Length=439, Percent_Identity=32.1184510250569, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1789941, Length=440, Percent_Identity=30.9090909090909, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI87082231, Length=214, Percent_Identity=28.0373831775701, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082404, Length=378, Percent_Identity=23.5449735449735, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46952; Mature: 46821

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PHPASEPHPASSATAPSRRRKRRTLSLGNLSAGTIGNVLEWYDFGLYGYLAPMIASLFF
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PSSDKIASLLGAYGGFAIGFMMRPIGGVIFGHLGDRVGRQTVLVTSVVMMGLATTAVGLL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
PTYQQIGFWAPVLLLVVRLFQGLSVGGEFTGSVSYLVETAPANRRGFAGSFANFGSTGGY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCCCCCCHHCCCCCCCH
LLAAGFAALTTLMAGHHAVQGWAWRVPFLAGGVLAVLAYLVRSRLTDTGYEPDKAAEDDE
HHHHHHHHHHHHHHHHHHHCCCEEECHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
LPIRQAFRRSPKIMLLAMLFTWGYGVADYLTLVFLPTFGSKFGAISDGLALEIVTVTQLF
CCHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
VLVLIPLTGWLTDHWILRRSWLIGNFGLLLVLALPFFAMAQGALTPFAIAQVVSGLMLGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IMGVAPAMLSEQFRSEYRLSGYSLAFNVGLGIGGGTAPLIATALIGLTGVKLAAGAYLLV
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GCAMSVVALWMLKDRGRQPLR
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]