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The map label for this gene is leuB [H]

Identifier: 148261138

GI number: 148261138

Start: 2371998

End: 2373110

Strand: Reverse

Name: leuB [H]

Synonym: Acry_2147

Alternate gene names: 148261138

Gene position: 2373110-2371998 (Counterclockwise)

Preceding gene: 148261139

Following gene: 148261136

Centisome position: 70.02

GC content: 69.27

Gene sequence:

>1113_bases
ATGATTGCGAACCGGAAACTGCTGCTCCTGCCGGGGGATGGCATCGGCCCCGAGGTGATGGCGGAAGCCCGCCGGGTGAT
CGACTGGCTGGCGCGCAGCGGCCGAGCCCGGTTCGAACTCGCCGAGGATCTCGTCGGCGGCGCCTCGATCGAGGCGACCG
GCAAGCCGATCACCGAACCGGTGATCGAGCGCGCGCTGGAGGCGGATGCCGTGCTGTTCGGCGCGGTGGGCGGGCCGCAA
TGGGATTCGCAGCCCTTCGACAACCGGCCGGAAATCGCCATTCTCGAACTGCGCAAGCGCCTCGGCCTGTTCGCCAACCT
GCGCCCCGCGCCGGTGTTCGACGCGCTGATCGACGCGAGCCCGCTCAAGCCCGAGATCGTGCGCGGGCTCGACATCATGA
TCGTCCGGGAATCGACGGGCGGCATCTATTTCGGCGAGCCGCGCGGGATCGAGACCCTGCCGGATGGCACGAAGCGCGGG
ATCAACACCGAGGTCTACACCACGCCGGAGATCGAGCGGGTGGCGCGCGTCGCCTTCGAGCTGGCGCGCAAGCGGCAGGG
GCGGCTGACCAGCGTCGAGAAGGCGAACGTGATGGAATCCGGCCTGCTCTGGCGGCAGACCGTCACCGCCCTCGGGGCGG
AGGAATATCCTGACGTGCAGCTCTCGCACATGTACGCCGACAATTGCGCGATGCAGCTGGCGAAGAATCCGCGCCAGTTC
GACGTGATCGTGACCGGCAACCTGTTCGGCGACCTGCTCTCCGACCTGGCCTCGATGCTGACCGGCTCGCTCGGGATGCT
GCCCTCGGCGACGCTCGGCGCGCCGGATGCGGCGGGGCGGCGGCATGCGCTCTACGAGCCGATCCACGGCTCGGCGCCGG
ACATCGCCGGCAAGGGCCTGGCCAATCCGCTGGCGCAGATCCTCAGCCTCGCGATGCTGCTGCGCTATTCCTTCGACATG
CAGGAGGATGCGGCGGCGATCGAAGCCGCCGTCTCCAACGTGCTCGCGAGCGGCATGCGCACCGCGGATATCGAGCAGAA
GGGCACGGCGCGGATCGGCACGCGCACGATGGGCGACGCGGTGCTGCGCGAGCTCGACAAGCTCGGCGGCTGA

Upstream 100 bases:

>100_bases
CGAAGCTGGACGGCGCGGCCTGGCGGCCGCGGATCGAGACCGTCTGATCCGCCCGCCCCGCTCCACCACCGCCGAACGCG
ACCCCAAGGGAGAGACGACC

Downstream 100 bases:

>100_bases
TCCGGGCCGGAAAAGGATCGGGGCGGCAGGTTTGCGCCTGCCGCCCCGTTTTCGTTTCAGCCGGAGGAAGCGCGGTCAGT
CGCCGGCCGCGGTGCGCGCG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MIANRKLLLLPGDGIGPEVMAEARRVIDWLARSGRARFELAEDLVGGASIEATGKPITEPVIERALEADAVLFGAVGGPQ
WDSQPFDNRPEIAILELRKRLGLFANLRPAPVFDALIDASPLKPEIVRGLDIMIVRESTGGIYFGEPRGIETLPDGTKRG
INTEVYTTPEIERVARVAFELARKRQGRLTSVEKANVMESGLLWRQTVTALGAEEYPDVQLSHMYADNCAMQLAKNPRQF
DVIVTGNLFGDLLSDLASMLTGSLGMLPSATLGAPDAAGRRHALYEPIHGSAPDIAGKGLANPLAQILSLAMLLRYSFDM
QEDAAAIEAAVSNVLASGMRTADIEQKGTARIGTRTMGDAVLRELDKLGG

Sequences:

>Translated_370_residues
MIANRKLLLLPGDGIGPEVMAEARRVIDWLARSGRARFELAEDLVGGASIEATGKPITEPVIERALEADAVLFGAVGGPQ
WDSQPFDNRPEIAILELRKRLGLFANLRPAPVFDALIDASPLKPEIVRGLDIMIVRESTGGIYFGEPRGIETLPDGTKRG
INTEVYTTPEIERVARVAFELARKRQGRLTSVEKANVMESGLLWRQTVTALGAEEYPDVQLSHMYADNCAMQLAKNPRQF
DVIVTGNLFGDLLSDLASMLTGSLGMLPSATLGAPDAAGRRHALYEPIHGSAPDIAGKGLANPLAQILSLAMLLRYSFDM
QEDAAAIEAAVSNVLASGMRTADIEQKGTARIGTRTMGDAVLRELDKLGG
>Mature_370_residues
MIANRKLLLLPGDGIGPEVMAEARRVIDWLARSGRARFELAEDLVGGASIEATGKPITEPVIERALEADAVLFGAVGGPQ
WDSQPFDNRPEIAILELRKRLGLFANLRPAPVFDALIDASPLKPEIVRGLDIMIVRESTGGIYFGEPRGIETLPDGTKRG
INTEVYTTPEIERVARVAFELARKRQGRLTSVEKANVMESGLLWRQTVTALGAEEYPDVQLSHMYADNCAMQLAKNPRQF
DVIVTGNLFGDLLSDLASMLTGSLGMLPSATLGAPDAAGRRHALYEPIHGSAPDIAGKGLANPLAQILSLAMLLRYSFDM
QEDAAAIEAAVSNVLASGMRTADIEQKGTARIGTRTMGDAVLRELDKLGG

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=339, Percent_Identity=34.2182890855457, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI28178816, Length=355, Percent_Identity=29.2957746478873, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI28178821, Length=319, Percent_Identity=30.7210031347962, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI4758582, Length=355, Percent_Identity=29.0140845070423, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI28178838, Length=331, Percent_Identity=29.3051359516616, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI28178819, Length=158, Percent_Identity=36.7088607594937, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI87081683, Length=365, Percent_Identity=51.2328767123288, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1788101, Length=374, Percent_Identity=34.2245989304813, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1787381, Length=401, Percent_Identity=25.1870324189526, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=352, Percent_Identity=32.3863636363636, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI25144293, Length=375, Percent_Identity=29.0666666666667, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17505779, Length=346, Percent_Identity=30.9248554913295, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17550882, Length=361, Percent_Identity=29.6398891966759, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319830, Length=373, Percent_Identity=42.3592493297587, Blast_Score=286, Evalue=5e-78,
Organism=Saccharomyces cerevisiae, GI6322097, Length=377, Percent_Identity=33.42175066313, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6324291, Length=342, Percent_Identity=31.2865497076023, Blast_Score=139, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6324709, Length=373, Percent_Identity=32.171581769437, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24643270, Length=354, Percent_Identity=31.0734463276836, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24643268, Length=354, Percent_Identity=31.0734463276836, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24661184, Length=372, Percent_Identity=31.7204301075269, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI281362242, Length=371, Percent_Identity=29.6495956873315, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648872, Length=371, Percent_Identity=29.6495956873315, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161078635, Length=348, Percent_Identity=27.5862068965517, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161078633, Length=348, Percent_Identity=27.5862068965517, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24650122, Length=348, Percent_Identity=27.5862068965517, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161078637, Length=348, Percent_Identity=27.5862068965517, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161078639, Length=346, Percent_Identity=27.7456647398844, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20130355, Length=361, Percent_Identity=24.3767313019391, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39823; Mature: 39823

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIANRKLLLLPGDGIGPEVMAEARRVIDWLARSGRARFELAEDLVGGASIEATGKPITEP
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEECCCCCCHHH
VIERALEADAVLFGAVGGPQWDSQPFDNRPEIAILELRKRLGLFANLRPAPVFDALIDAS
HHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCC
PLKPEIVRGLDIMIVRESTGGIYFGEPRGIETLPDGTKRGINTEVYTTPEIERVARVAFE
CCCHHHHCCCEEEEEEECCCCEEECCCCCCCCCCCCHHCCCCCEEEECHHHHHHHHHHHH
LARKRQGRLTSVEKANVMESGLLWRQTVTALGAEEYPDVQLSHMYADNCAMQLAKNPRQF
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
DVIVTGNLFGDLLSDLASMLTGSLGMLPSATLGAPDAAGRRHALYEPIHGSAPDIAGKGL
EEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
ANPLAQILSLAMLLRYSFDMQEDAAAIEAAVSNVLASGMRTADIEQKGTARIGTRTMGDA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
VLRELDKLGG
HHHHHHHHCC
>Mature Secondary Structure
MIANRKLLLLPGDGIGPEVMAEARRVIDWLARSGRARFELAEDLVGGASIEATGKPITEP
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEECCCCCCHHH
VIERALEADAVLFGAVGGPQWDSQPFDNRPEIAILELRKRLGLFANLRPAPVFDALIDAS
HHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCC
PLKPEIVRGLDIMIVRESTGGIYFGEPRGIETLPDGTKRGINTEVYTTPEIERVARVAFE
CCCHHHHCCCEEEEEEECCCCEEECCCCCCCCCCCCHHCCCCCEEEECHHHHHHHHHHHH
LARKRQGRLTSVEKANVMESGLLWRQTVTALGAEEYPDVQLSHMYADNCAMQLAKNPRQF
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
DVIVTGNLFGDLLSDLASMLTGSLGMLPSATLGAPDAAGRRHALYEPIHGSAPDIAGKGL
EEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
ANPLAQILSLAMLLRYSFDMQEDAAAIEAAVSNVLASGMRTADIEQKGTARIGTRTMGDA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
VLRELDKLGG
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA