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The map label for this gene is rlmE

Identifier: 148261003

GI number: 148261003

Start: 2205696

End: 2206373

Strand: Reverse

Name: rlmE

Synonym: Acry_2011

Alternate gene names: 148261003

Gene position: 2206373-2205696 (Counterclockwise)

Preceding gene: 148261004

Following gene: 148260999

Centisome position: 65.1

GC content: 66.08

Gene sequence:

>678_bases
ATGACCGAGGAAACCATCGGCTCGCGCCGCCGCGCCGCCGTGCGGCTCAAGGCCGCGCGCAAGCACAAGCCCTCGTCGCA
GAAATGGCTGCTCCGCCAGTTGAACGACCCTTATGTCGCCGCGGCGAAGGAGCGGGGATTCCGTTCCCGAGCGGCGTTCA
AGCTCATCGAGCTCGACGAGAAATTCGGACTGATCGGCAAGGGGGCGCGGGTGGTGGATCTCGGTGCCGCTCCCGGCGGC
TGGACGCAGGTCGCACTTGAGCGCGGCGCGTCACGCGTCGTCGGCATCGACCTGCTGGACATCGACCCGATCGCGGGGGC
AACCCTGATCAAGGGTGATTTCCAGGACGAATCGATGGAGACGGCACTTGCCGAGATACTGGGCGGACCTGCCGATGTCG
TGATGTCCGACATGGCGCCCAACACCACCGGCCATACCGCGACCGACCATCTCAGGATCATGGGCCTCGCCGAACTCGCG
CTCGATTTCGCCTTCAAGCATCTCGCGCCTGGCGGTGCCTTCGTGACCAAGCTGTTTCAGGGTGGGGCGCAGGGCGACAT
GCTGAACCTGCTCAAGCGCCGCTTCGCCCAGGTGCGCCATGCGAAGCCCGAGGCGAGCCGCAAGGATAGCCGCGAGCTTT
ACCTCGTCGCCACCGGTTACCGCCCCGACGCATCCTGA

Upstream 100 bases:

>100_bases
GGCCGGCCGCCGATCTCGCTGTGGCCGACCGCGGCCTGCGCGAGGGGATGCTGATGCGCCTCATCCGCAACGCGCGTTCA
CGTCCGCGCCGCTGCCAGGC

Downstream 100 bases:

>100_bases
GACGCGAGATGCCGCCGCAGTTCCGCGCGGCACGCATCCCCGTCCGGCAGACAATCGTGCGCCATCCACCATCTGCGCAC
CGCGGCGAGCGCGGCGCCGA

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTEETIGSRRRAAVRLKAARKHKPSSQKWLLRQLNDPYVAAAKERGFRSRAAFKLIELDEKFGLIGKGARVVDLGAAPGG
WTQVALERGASRVVGIDLLDIDPIAGATLIKGDFQDESMETALAEILGGPADVVMSDMAPNTTGHTATDHLRIMGLAELA
LDFAFKHLAPGGAFVTKLFQGGAQGDMLNLLKRRFAQVRHAKPEASRKDSRELYLVATGYRPDAS

Sequences:

>Translated_225_residues
MTEETIGSRRRAAVRLKAARKHKPSSQKWLLRQLNDPYVAAAKERGFRSRAAFKLIELDEKFGLIGKGARVVDLGAAPGG
WTQVALERGASRVVGIDLLDIDPIAGATLIKGDFQDESMETALAEILGGPADVVMSDMAPNTTGHTATDHLRIMGLAELA
LDFAFKHLAPGGAFVTKLFQGGAQGDMLNLLKRRFAQVRHAKPEASRKDSRELYLVATGYRPDAS
>Mature_224_residues
TEETIGSRRRAAVRLKAARKHKPSSQKWLLRQLNDPYVAAAKERGFRSRAAFKLIELDEKFGLIGKGARVVDLGAAPGGW
TQVALERGASRVVGIDLLDIDPIAGATLIKGDFQDESMETALAEILGGPADVVMSDMAPNTTGHTATDHLRIMGLAELAL
DFAFKHLAPGGAFVTKLFQGGAQGDMLNLLKRRFAQVRHAKPEASRKDSRELYLVATGYRPDAS

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=229, Percent_Identity=39.7379912663755, Blast_Score=157, Evalue=7e-39,
Organism=Homo sapiens, GI29029591, Length=192, Percent_Identity=39.5833333333333, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI29029589, Length=192, Percent_Identity=39.5833333333333, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI7110661, Length=192, Percent_Identity=39.5833333333333, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI194097365, Length=193, Percent_Identity=38.860103626943, Blast_Score=126, Evalue=1e-29,
Organism=Escherichia coli, GI1789569, Length=206, Percent_Identity=46.6019417475728, Blast_Score=181, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17553474, Length=211, Percent_Identity=35.5450236966825, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71987561, Length=196, Percent_Identity=38.265306122449, Blast_Score=125, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71987550, Length=196, Percent_Identity=38.265306122449, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17553860, Length=196, Percent_Identity=38.265306122449, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17554650, Length=199, Percent_Identity=33.6683417085427, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321302, Length=287, Percent_Identity=30.6620209059233, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319535, Length=203, Percent_Identity=33.4975369458128, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319796, Length=190, Percent_Identity=33.6842105263158, Blast_Score=104, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356387, Length=209, Percent_Identity=40.1913875598086, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=42.0212765957447, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24648639, Length=204, Percent_Identity=36.7647058823529, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=33.8383838383838, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_ACICJ (A5G029)

Other databases:

- EMBL:   CP000697
- RefSeq:   YP_001235130.1
- ProteinModelPortal:   A5G029
- SMR:   A5G029
- STRING:   A5G029
- GeneID:   5161183
- GenomeReviews:   CP000697_GR
- KEGG:   acr:Acry_2011
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- BioCyc:   ACRY349163:ACRY_2011-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 24378; Mature: 24247

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: NA

Important sites: ACT_SITE 177-177 BINDING 79-79 BINDING 81-81 BINDING 97-97 BINDING 113-113 BINDING 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEETIGSRRRAAVRLKAARKHKPSSQKWLLRQLNDPYVAAAKERGFRSRAAFKLIELDE
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEHHHHCCCHHHHHHHHEEHHH
KFGLIGKGARVVDLGAAPGGWTQVALERGASRVVGIDLLDIDPIAGATLIKGDFQDESME
HCCCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCEEEECCCCCHHHH
TALAEILGGPADVVMSDMAPNTTGHTATDHLRIMGLAELALDFAFKHLAPGGAFVTKLFQ
HHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
GGAQGDMLNLLKRRFAQVRHAKPEASRKDSRELYLVATGYRPDAS
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
TEETIGSRRRAAVRLKAARKHKPSSQKWLLRQLNDPYVAAAKERGFRSRAAFKLIELDE
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEHHHHCCCHHHHHHHHEEHHH
KFGLIGKGARVVDLGAAPGGWTQVALERGASRVVGIDLLDIDPIAGATLIKGDFQDESME
HCCCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCEEEECCCCCHHHH
TALAEILGGPADVVMSDMAPNTTGHTATDHLRIMGLAELALDFAFKHLAPGGAFVTKLFQ
HHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
GGAQGDMLNLLKRRFAQVRHAKPEASRKDSRELYLVATGYRPDAS
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA