Click here to switch to the map view.

The map label for this gene is kefB [H]

Identifier: 148260973

GI number: 148260973

Start: 2170924

End: 2172735

Strand: Reverse

Name: kefB [H]

Synonym: Acry_1980

Alternate gene names: 148260973

Gene position: 2172735-2170924 (Counterclockwise)

Preceding gene: 148260977

Following gene: 148260970

Centisome position: 64.11

GC content: 69.81

Gene sequence:

>1812_bases
ATGATCATCACCCTCGTCGCACTGCTCGCGGCCACGGTCGTCACGGTGCCGCTGGCGCGTCGCCTCGGGGTCGGCAGCAT
CGTGGGCTATCTGTTCGCCGGGCTCATCATCGGTCCGGCCGGCCTGCGCCTCGTCACCGGCATCTCCGAAATCCGCAGCG
TGTCGGAGCTCGGGGTGACCATGCTGCTGTTCCTGATCGGGCTGGAGATTCGCCCGCACCGGCTCTGGATCCTGCGCAAG
ACCATCCTGGGCCTCGGTTTCGGTCACATGATCCCGAGCGCCGCCATTCTGGCCGTCCTTGCCCATTTCGCCGGACTCGG
CTGGCCGGCGGCGACGGTGGTCGGCCTCGGCCTGTCGCTCTCCTCCACGGCCATCGTCCTGCCGATGCTGAACGAGCGCA
ACCTGCTCAGCGCCGCCTCCGGCCGCGATGCCTTCGCCGTCCTGCTGTTCCAGGATATGGCCTTCATCCCCCTGGTCGCC
CTGGTGCCGCTGCTCGTCGGCAATACGCTGGCGAGCCATCTCGCCGCCGGCGCGGCCCCGGCGGATGTCGCCGCGCATTT
CGCCCACAATGTCCCCTGGCTCGCGGTCGGGCGGGCGGTGCTGGCGGTGGCGGCGATCCTGTTCGGCGGCATGGTCCTCG
CCGACCGCGTCTTCCGCCTGATCGGCGGTGCGCGCACCCCCGAGGTGTTCACCGCGATGACGCTGCTGATCGTCGTCGGC
ACGGCGGCTCTGGCCCACTGGGCCGGACTGTCGGCCTCGCTCGGCGCCTTCATGGCGGGCGCGCTGCTGTCGGATTCGGA
GTTCCGTCACGCCATCCAGGCCGATATCGAGCCGTTCGAGGGCCTGCTGCTCGGCTTCTTCTTCATCTCGGTTGGCATGT
CGGTCGATTTCTCGCTGTTTCATAGCGAACCGCTGCTGATCGTCGCTTCCGTGGCCGCGCTGCTTGTCGTGAAGACCGCG
GTCGGGTTCGTGGTCGCCTTCGCGAAGCGCCGCAACGTGGCCACCGCGCTGCGCTTTGCCGCTGCATTGCCGGAAGGCAG
CGAGTTCAGCTTTGTGGTCTTCGCCGCCGCCGTCGGTGCCGGGGTGCTGAACCGGGCCCAGTCTGGGCTGGCGACGCTCA
CTGTCGCCCTGTCGATGTTGGTGGTGCCGCTCGGTTTCGCCGGCATCGAGCGCTGGGTCATGCCGCGCATCGCCCGCCGC
CAGACGCCACCGACCACCCCGATCGAGGAGGGCGCCGTCGTCCCGGTGCTGATCTGCGGCTTCGGCCGGGTCGGCCAGAT
CGTCGGCCGGCTGCTCTCGGTGCGACGGATTCCCTTCGTCGCCCTCGACCAGAACGCGGAACATGTCGCCTTCGTCCGCC
GCTTCGGCGCGCGGGCCTATTTCGGCGATCCGGCCCGGCTCACCGTCCTGCGCGCCGCCGGCGCGGGCAATGCGAAGCTG
CTGGTCATCGCACTCGACGATCCCGATGCGGCGCTCCGGGTGGCCGACCTCGCGCGCCGCCACCTGCCGCACCTGACCAT
CCTGGCGCGGGCGCGGAACCGCCGCCATGCCCAGTTGCTGATGGATCGCGGCGTCGAGCACATCGTGCGCGAAACCTTTG
CATCGAGCCTCGAACTCGGCGAACGCGCGCTGCGCGAACTCGGCATTCCGGCCGAGGAGGCGGCGCGCACCATCTTCACG
TTCCGGCAGCGGGACGAGGCGCTGCTCACCGAAACCCTCGGCTTTGCCGGCGACCCGTCGCGCGTGGTCGCCAGCATCGA
GCAGAGCGTCGCCGAACTGCAGTCCCTCTTCGAGGCCGATCGCGGCGGCTGA

Upstream 100 bases:

>100_bases
GTGGCAGGGTCATTCCGCCGGTCCGCTTCCGCGCGCCGGTACTGCCTGACCATCCGCGGCCCACAATCCACGCTCATGCC
TGTTTCGGGGAACCGGCCCA

Downstream 100 bases:

>100_bases
GTTGGGCTATTTCATCGCCTTCTGCCGGGCCACCAGCGCGTCGACGATCTTCGGGATGTCGGCCTCCGCGTAGGGCGCGC
CGAACGCTTTCTTCATCTTC

Product: sodium/hydrogen exchanger

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MIITLVALLAATVVTVPLARRLGVGSIVGYLFAGLIIGPAGLRLVTGISEIRSVSELGVTMLLFLIGLEIRPHRLWILRK
TILGLGFGHMIPSAAILAVLAHFAGLGWPAATVVGLGLSLSSTAIVLPMLNERNLLSAASGRDAFAVLLFQDMAFIPLVA
LVPLLVGNTLASHLAAGAAPADVAAHFAHNVPWLAVGRAVLAVAAILFGGMVLADRVFRLIGGARTPEVFTAMTLLIVVG
TAALAHWAGLSASLGAFMAGALLSDSEFRHAIQADIEPFEGLLLGFFFISVGMSVDFSLFHSEPLLIVASVAALLVVKTA
VGFVVAFAKRRNVATALRFAAALPEGSEFSFVVFAAAVGAGVLNRAQSGLATLTVALSMLVVPLGFAGIERWVMPRIARR
QTPPTTPIEEGAVVPVLICGFGRVGQIVGRLLSVRRIPFVALDQNAEHVAFVRRFGARAYFGDPARLTVLRAAGAGNAKL
LVIALDDPDAALRVADLARRHLPHLTILARARNRRHAQLLMDRGVEHIVRETFASSLELGERALRELGIPAEEAARTIFT
FRQRDEALLTETLGFAGDPSRVVASIEQSVAELQSLFEADRGG

Sequences:

>Translated_603_residues
MIITLVALLAATVVTVPLARRLGVGSIVGYLFAGLIIGPAGLRLVTGISEIRSVSELGVTMLLFLIGLEIRPHRLWILRK
TILGLGFGHMIPSAAILAVLAHFAGLGWPAATVVGLGLSLSSTAIVLPMLNERNLLSAASGRDAFAVLLFQDMAFIPLVA
LVPLLVGNTLASHLAAGAAPADVAAHFAHNVPWLAVGRAVLAVAAILFGGMVLADRVFRLIGGARTPEVFTAMTLLIVVG
TAALAHWAGLSASLGAFMAGALLSDSEFRHAIQADIEPFEGLLLGFFFISVGMSVDFSLFHSEPLLIVASVAALLVVKTA
VGFVVAFAKRRNVATALRFAAALPEGSEFSFVVFAAAVGAGVLNRAQSGLATLTVALSMLVVPLGFAGIERWVMPRIARR
QTPPTTPIEEGAVVPVLICGFGRVGQIVGRLLSVRRIPFVALDQNAEHVAFVRRFGARAYFGDPARLTVLRAAGAGNAKL
LVIALDDPDAALRVADLARRHLPHLTILARARNRRHAQLLMDRGVEHIVRETFASSLELGERALRELGIPAEEAARTIFT
FRQRDEALLTETLGFAGDPSRVVASIEQSVAELQSLFEADRGG
>Mature_603_residues
MIITLVALLAATVVTVPLARRLGVGSIVGYLFAGLIIGPAGLRLVTGISEIRSVSELGVTMLLFLIGLEIRPHRLWILRK
TILGLGFGHMIPSAAILAVLAHFAGLGWPAATVVGLGLSLSSTAIVLPMLNERNLLSAASGRDAFAVLLFQDMAFIPLVA
LVPLLVGNTLASHLAAGAAPADVAAHFAHNVPWLAVGRAVLAVAAILFGGMVLADRVFRLIGGARTPEVFTAMTLLIVVG
TAALAHWAGLSASLGAFMAGALLSDSEFRHAIQADIEPFEGLLLGFFFISVGMSVDFSLFHSEPLLIVASVAALLVVKTA
VGFVVAFAKRRNVATALRFAAALPEGSEFSFVVFAAAVGAGVLNRAQSGLATLTVALSMLVVPLGFAGIERWVMPRIARR
QTPPTTPIEEGAVVPVLICGFGRVGQIVGRLLSVRRIPFVALDQNAEHVAFVRRFGARAYFGDPARLTVLRAAGAGNAKL
LVIALDDPDAALRVADLARRHLPHLTILARARNRRHAQLLMDRGVEHIVRETFASSLELGERALRELGIPAEEAARTIFT
FRQRDEALLTETLGFAGDPSRVVASIEQSVAELQSLFEADRGG

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=417, Percent_Identity=26.8585131894484, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI1789749, Length=595, Percent_Identity=39.4957983193277, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1786232, Length=601, Percent_Identity=39.6006655574043, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1786685, Length=553, Percent_Identity=27.6672694394213, Blast_Score=164, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 63700; Mature: 63700

Theoretical pI: Translated: 10.88; Mature: 10.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIITLVALLAATVVTVPLARRLGVGSIVGYLFAGLIIGPAGLRLVTGISEIRSVSELGVT
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
MLLFLIGLEIRPHRLWILRKTILGLGFGHMIPSAAILAVLAHFAGLGWPAATVVGLGLSL
HHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
SSTAIVLPMLNERNLLSAASGRDAFAVLLFQDMAFIPLVALVPLLVGNTLASHLAAGAAP
CCCEEEEEECCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ADVAAHFAHNVPWLAVGRAVLAVAAILFGGMVLADRVFRLIGGARTPEVFTAMTLLIVVG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TAALAHWAGLSASLGAFMAGALLSDSEFRHAIQADIEPFEGLLLGFFFISVGMSVDFSLF
HHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHH
HSEPLLIVASVAALLVVKTAVGFVVAFAKRRNVATALRFAAALPEGSEFSFVVFAAAVGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
GVLNRAQSGLATLTVALSMLVVPLGFAGIERWVMPRIARRQTPPTTPIEEGAVVPVLICG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHC
FGRVGQIVGRLLSVRRIPFVALDQNAEHVAFVRRFGARAYFGDPARLTVLRAAGAGNAKL
CCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCCCHHEEEEEECCCCCCEE
LVIALDDPDAALRVADLARRHLPHLTILARARNRRHAQLLMDRGVEHIVRETFASSLELG
EEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ERALRELGIPAEEAARTIFTFRQRDEALLTETLGFAGDPSRVVASIEQSVAELQSLFEAD
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
RGG
CCC
>Mature Secondary Structure
MIITLVALLAATVVTVPLARRLGVGSIVGYLFAGLIIGPAGLRLVTGISEIRSVSELGVT
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
MLLFLIGLEIRPHRLWILRKTILGLGFGHMIPSAAILAVLAHFAGLGWPAATVVGLGLSL
HHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
SSTAIVLPMLNERNLLSAASGRDAFAVLLFQDMAFIPLVALVPLLVGNTLASHLAAGAAP
CCCEEEEEECCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ADVAAHFAHNVPWLAVGRAVLAVAAILFGGMVLADRVFRLIGGARTPEVFTAMTLLIVVG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TAALAHWAGLSASLGAFMAGALLSDSEFRHAIQADIEPFEGLLLGFFFISVGMSVDFSLF
HHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHH
HSEPLLIVASVAALLVVKTAVGFVVAFAKRRNVATALRFAAALPEGSEFSFVVFAAAVGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
GVLNRAQSGLATLTVALSMLVVPLGFAGIERWVMPRIARRQTPPTTPIEEGAVVPVLICG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHC
FGRVGQIVGRLLSVRRIPFVALDQNAEHVAFVRRFGARAYFGDPARLTVLRAAGAGNAKL
CCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCCCHHEEEEEECCCCCCEE
LVIALDDPDAALRVADLARRHLPHLTILARARNRRHAQLLMDRGVEHIVRETFASSLELG
EEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ERALRELGIPAEEAARTIFTFRQRDEALLTETLGFAGDPSRVVASIEQSVAELQSLFEAD
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
RGG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA