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The map label for this gene is xylF [H]

Identifier: 148260966

GI number: 148260966

Start: 2164103

End: 2165212

Strand: Reverse

Name: xylF [H]

Synonym: Acry_1973

Alternate gene names: 148260966

Gene position: 2165212-2164103 (Counterclockwise)

Preceding gene: 148260967

Following gene: 148260965

Centisome position: 63.89

GC content: 65.14

Gene sequence:

>1110_bases
ATGAAAATCGCGTTCCCGACGATTATTGCCGCGTCCGCGCTGGCGGTTGGCGCCTTCGGCCTGACGGCCGCGCACGCCCA
GTCGGTCACCCAGCCTTCCGTGCACCAGATCAGCAGCAAGAAGACGACGATCGCCTTCCTGCTGCCCGAGAACTCCTCGC
CGCGCTATGAGCAGGTCGACCGTCCCTGGTTCATCAAGATGGCGCATGCGGCCGATCCGAACGCCAAGCTGATCGTCAGC
AACGCCAATTCGAGCCCCGACACCCAGCTCAACCAGGCCGAATCCGCGATCTCGAACGGCGCGGACGTTCTCGTCGTCGA
TCCGGTCGACGGCAAGGCCGGTGCCGCGATCGTCAACTACGCCGCGCGCAACCACGTCAAGGTCATTTCCTACGACCGTC
TGATCCAGGATTCGAAGCCCGATTACTACGTCTCGTTCGACAACACGAAGGTCGGCGAGCTGCAGGGCGAGTATATCGCC
AAGCACACCAAGCCCGGCGGCAGCGTGATGATGATCAACGGCGCGCCGACCGATCCGAACGCGTTCGACTTCCGCAAGGG
CGCGATGAACGTGCTCGGCCCGCTCTTCAAATCCGGCAAGCTGCACCTCGCCTACTCGGTGATGACGCCGGACTGGAGCC
CGCAGAACGCGTTCGAGGAAAGCCAGCAGGCGCTCACCCGGCTGCACAACAAGGTCAACGCCGTCCTCGCCGCGAATGAC
GGCACCGCCTCCGGCGTGATCCGCGCGCTCCAGGCGGTCGGCCTCGCCGGCAAGGTGCCGGTGACCGGCCAGGATGCGAC
CAATGGCGGCCTGACCTTCATCCTCGAAGGCCTGCAGTCGATGACCGTGTTCAAATACGTCCCCGAGGAAGCCGCCGCGG
CGGCGGAACTCGCGGTGGCGGTGGCGACCGACACGAAGCCGCCGGCCGGCCTGCTGAACGGCAAGACCAACAACAAGATG
ATCGACGTCCCGTCGGTCCTGCTGAAGCCGCTCGTGGTCACCAAGGCGAATATCGCCGGCTCGGTGATCAAGTCGGGCTA
TACCACCTGGAAGGCGATCTGTGTGGGCCCCGCGGCCCAGGGCAAGATCTGCAAGGCCCACGGCGCCTGA

Upstream 100 bases:

>100_bases
CGATAGCAACCGATTTACTACAAGGGTCAAATTAATTGGAGCTCCGCGGTTCGGCGGAGGACCGGAATTCGATCCATCAT
TTCTTGGAAGAGGAAACACC

Downstream 100 bases:

>100_bases
TCCGGGACCATTGACGGCGCGGCGGACGCCCCGCGCGTCCGCCGTTTGCTTGGGAGGACGAGAGATGAGTGCGGCAATCG
CGGCTGAACCCGCCGCAGCA

Product: monosaccharide-transporting ATPase

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKIAFPTIIAASALAVGAFGLTAAHAQSVTQPSVHQISSKKTTIAFLLPENSSPRYEQVDRPWFIKMAHAADPNAKLIVS
NANSSPDTQLNQAESAISNGADVLVVDPVDGKAGAAIVNYAARNHVKVISYDRLIQDSKPDYYVSFDNTKVGELQGEYIA
KHTKPGGSVMMINGAPTDPNAFDFRKGAMNVLGPLFKSGKLHLAYSVMTPDWSPQNAFEESQQALTRLHNKVNAVLAAND
GTASGVIRALQAVGLAGKVPVTGQDATNGGLTFILEGLQSMTVFKYVPEEAAAAAELAVAVATDTKPPAGLLNGKTNNKM
IDVPSVLLKPLVVTKANIAGSVIKSGYTTWKAICVGPAAQGKICKAHGA

Sequences:

>Translated_369_residues
MKIAFPTIIAASALAVGAFGLTAAHAQSVTQPSVHQISSKKTTIAFLLPENSSPRYEQVDRPWFIKMAHAADPNAKLIVS
NANSSPDTQLNQAESAISNGADVLVVDPVDGKAGAAIVNYAARNHVKVISYDRLIQDSKPDYYVSFDNTKVGELQGEYIA
KHTKPGGSVMMINGAPTDPNAFDFRKGAMNVLGPLFKSGKLHLAYSVMTPDWSPQNAFEESQQALTRLHNKVNAVLAAND
GTASGVIRALQAVGLAGKVPVTGQDATNGGLTFILEGLQSMTVFKYVPEEAAAAAELAVAVATDTKPPAGLLNGKTNNKM
IDVPSVLLKPLVVTKANIAGSVIKSGYTTWKAICVGPAAQGKICKAHGA
>Mature_369_residues
MKIAFPTIIAASALAVGAFGLTAAHAQSVTQPSVHQISSKKTTIAFLLPENSSPRYEQVDRPWFIKMAHAADPNAKLIVS
NANSSPDTQLNQAESAISNGADVLVVDPVDGKAGAAIVNYAARNHVKVISYDRLIQDSKPDYYVSFDNTKVGELQGEYIA
KHTKPGGSVMMINGAPTDPNAFDFRKGAMNVLGPLFKSGKLHLAYSVMTPDWSPQNAFEESQQALTRLHNKVNAVLAAND
GTASGVIRALQAVGLAGKVPVTGQDATNGGLTFILEGLQSMTVFKYVPEEAAAAAELAVAVATDTKPPAGLLNGKTNNKM
IDVPSVLLKPLVVTKANIAGSVIKSGYTTWKAICVGPAAQGKICKAHGA

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=309, Percent_Identity=41.1003236245955, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1788473, Length=278, Percent_Identity=26.6187050359712, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790192, Length=248, Percent_Identity=25.4032258064516, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38702; Mature: 38702

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAFPTIIAASALAVGAFGLTAAHAQSVTQPSVHQISSKKTTIAFLLPENSSPRYEQVD
CEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEECCCCCCHHHCC
RPWFIKMAHAADPNAKLIVSNANSSPDTQLNQAESAISNGADVLVVDPVDGKAGAAIVNY
CCEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHH
AARNHVKVISYDRLIQDSKPDYYVSFDNTKVGELQGEYIAKHTKPGGSVMMINGAPTDPN
HHCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCHHHHEECCCCCCEEEEECCCCCCC
AFDFRKGAMNVLGPLFKSGKLHLAYSVMTPDWSPQNAFEESQQALTRLHNKVNAVLAAND
CCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEECC
GTASGVIRALQAVGLAGKVPVTGQDATNGGLTFILEGLQSMTVFKYVPEEAAAAAELAVA
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCHHHHHHHHEEEE
VATDTKPPAGLLNGKTNNKMIDVPSVLLKPLVVTKANIAGSVIKSGYTTWKAICVGPAAQ
EEECCCCCCCCCCCCCCCEEEECHHHHHCCHHEEECHHHHHHHHCCCCEEEEEEECCCCC
GKICKAHGA
CCEEECCCC
>Mature Secondary Structure
MKIAFPTIIAASALAVGAFGLTAAHAQSVTQPSVHQISSKKTTIAFLLPENSSPRYEQVD
CEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEECCCCCCHHHCC
RPWFIKMAHAADPNAKLIVSNANSSPDTQLNQAESAISNGADVLVVDPVDGKAGAAIVNY
CCEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHH
AARNHVKVISYDRLIQDSKPDYYVSFDNTKVGELQGEYIAKHTKPGGSVMMINGAPTDPN
HHCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCHHHHEECCCCCCEEEEECCCCCCC
AFDFRKGAMNVLGPLFKSGKLHLAYSVMTPDWSPQNAFEESQQALTRLHNKVNAVLAAND
CCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEECC
GTASGVIRALQAVGLAGKVPVTGQDATNGGLTFILEGLQSMTVFKYVPEEAAAAAELAVA
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCHHHHHHHHEEEE
VATDTKPPAGLLNGKTNNKMIDVPSVLLKPLVVTKANIAGSVIKSGYTTWKAICVGPAAQ
EEECCCCCCCCCCCCCCCEEEECHHHHHCCHHEEECHHHHHHHHCCCCEEEEEEECCCCC
GKICKAHGA
CCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]