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The map label for this gene is gltX

Identifier: 148260952

GI number: 148260952

Start: 2148736

End: 2150136

Strand: Reverse

Name: gltX

Synonym: Acry_1959

Alternate gene names: 148260952

Gene position: 2150136-2148736 (Counterclockwise)

Preceding gene: 148260953

Following gene: 148260950

Centisome position: 63.44

GC content: 68.67

Gene sequence:

>1401_bases
ATGACAATCCGCGTCCGTTTCGCGCCGTCGCCCACCGGCATGCTCCATATCGGCGGCGCCCGCACCGCCCTGTTCAACTA
TCTCTTCGCCCGCCATCACGGCGGCCAGTTCCTGCTCCGCGTCGAGGATACCGATCGCGAGCGCAGCACGCCCGAGGCAA
CGAAGGTCATCCTCGACGCGCTCGACTGGCTCGACCTGAAGCCCGACGAACCGCCGGTCTATCAATCGACCCGCTATGCC
CGCCATCGCGAGGTGGCCGAGCAGATGCTCGCCGCCGGCCAGGCCTATCGCTGCTACTGCTCCCGCGAGGAGCTTGCCGA
GATGCGCGCCGAGGCCGAGCGGAGCAAAAAGCCGTTCCGCTATGACGGGCGCTGGCGCGACCGCGATCCGGCCGAAGCGC
CGGCCGGCGTCGATCCCGTCATCCGCCTGCGCGCCCCGCGCGAGGGCGAGACCGTGATCCACGATCTCGTCCAGGGCGAG
GTGCGGGTGAAGAACGCCGAGCTGGACGACATGATCCTGCTGCGCTCCGACGGCACGCCGACCTATCTGCACGCCGTCGT
GGTGGATGACCACGACATGGGCATCACCCATGTCATCCGCGGCGACGACCATCTGACCAACACGTTCCGCCAGGCCCAGA
TCTACGACGCGATGGGCTGGGCGCGGCCGAATTTCGCCCATATCCCCCTGATCCACGGCGCGGACGGTGCGAAACTCTCG
AAGCGTCACGGCGCCGTCTCGGTCCTGCAGTTCCGCGACGAGGGCTATCTGCCCGAGGCGCTCTGCAACTACCTGCTCCG
CCTCGGCTGGGGCCATGGCGATGCGGAGATCCTGCCCCGCGAGGAGCAGGTCGCGCTGTTCGACCTCGATGGCGTCGGCC
GCGCCGCGAGCCGGATGGACTATGCCAAGCTGCTCCACGTCAACGCCGTCTTCCTCCGCGCCGCCGAGGACGAACGCCTT
GCCGCCGATGTCGTCGCCCGGCTCGAACGCGATCACGGTTTTCCGGTGAGCACCGAGGCGGCGGGCCGCATCGCGATGCT
GATGCCGGGGCTGAAGGACCGCGCCCGCACCCTGGCCGAACTGGCGGAATCCGCCCTGTTCGTCGTTCGTGAGGCGCCGC
TGCCGATGACCGAGAAGGCCGAAGCCCTGCTCACCGAGGCGGCGCGCGACGACCTCGCCGCCCTGCTGCTCGATTTCGAC
AAGACCGATTTCTCGAAGGCGGCGCTGCACGAGGCGATGAAGGCCTATGCCGAGCGCGAGGAGAAAAAGCTCGGCGCCAT
TGCCCAGCCGCTCCGCGCGGCGCTGACCGGCAGCACCGTCAGCCCGCCGATCGACGCGGTGATGGAAGCGCTCGGCCCGA
TCGAGGTCCGCAAGCGGATCTTTTCCGTCCTCGGCGAATAG

Upstream 100 bases:

>100_bases
GGCCGATTGCGCGGCCGCCGGGCTCGATCCGCGCGCCATCATCGGCGGGTTCCGCTGGTAAGGGTTCATCCCCGCGCCGT
TTCGTGATAGCGCCGGGGTC

Downstream 100 bases:

>100_bases
CGCCGCCGTTGCCGCCCGAAAAAATGGCGGGCGCCGCAAGGCCGCCCGCCATTCGCGTTTCAGCCGGGGAGGGAACGGAA
CATCAGCGATTGACCGCCTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MTIRVRFAPSPTGMLHIGGARTALFNYLFARHHGGQFLLRVEDTDRERSTPEATKVILDALDWLDLKPDEPPVYQSTRYA
RHREVAEQMLAAGQAYRCYCSREELAEMRAEAERSKKPFRYDGRWRDRDPAEAPAGVDPVIRLRAPREGETVIHDLVQGE
VRVKNAELDDMILLRSDGTPTYLHAVVVDDHDMGITHVIRGDDHLTNTFRQAQIYDAMGWARPNFAHIPLIHGADGAKLS
KRHGAVSVLQFRDEGYLPEALCNYLLRLGWGHGDAEILPREEQVALFDLDGVGRAASRMDYAKLLHVNAVFLRAAEDERL
AADVVARLERDHGFPVSTEAAGRIAMLMPGLKDRARTLAELAESALFVVREAPLPMTEKAEALLTEAARDDLAALLLDFD
KTDFSKAALHEAMKAYAEREEKKLGAIAQPLRAALTGSTVSPPIDAVMEALGPIEVRKRIFSVLGE

Sequences:

>Translated_466_residues
MTIRVRFAPSPTGMLHIGGARTALFNYLFARHHGGQFLLRVEDTDRERSTPEATKVILDALDWLDLKPDEPPVYQSTRYA
RHREVAEQMLAAGQAYRCYCSREELAEMRAEAERSKKPFRYDGRWRDRDPAEAPAGVDPVIRLRAPREGETVIHDLVQGE
VRVKNAELDDMILLRSDGTPTYLHAVVVDDHDMGITHVIRGDDHLTNTFRQAQIYDAMGWARPNFAHIPLIHGADGAKLS
KRHGAVSVLQFRDEGYLPEALCNYLLRLGWGHGDAEILPREEQVALFDLDGVGRAASRMDYAKLLHVNAVFLRAAEDERL
AADVVARLERDHGFPVSTEAAGRIAMLMPGLKDRARTLAELAESALFVVREAPLPMTEKAEALLTEAARDDLAALLLDFD
KTDFSKAALHEAMKAYAEREEKKLGAIAQPLRAALTGSTVSPPIDAVMEALGPIEVRKRIFSVLGE
>Mature_465_residues
TIRVRFAPSPTGMLHIGGARTALFNYLFARHHGGQFLLRVEDTDRERSTPEATKVILDALDWLDLKPDEPPVYQSTRYAR
HREVAEQMLAAGQAYRCYCSREELAEMRAEAERSKKPFRYDGRWRDRDPAEAPAGVDPVIRLRAPREGETVIHDLVQGEV
RVKNAELDDMILLRSDGTPTYLHAVVVDDHDMGITHVIRGDDHLTNTFRQAQIYDAMGWARPNFAHIPLIHGADGAKLSK
RHGAVSVLQFRDEGYLPEALCNYLLRLGWGHGDAEILPREEQVALFDLDGVGRAASRMDYAKLLHVNAVFLRAAEDERLA
ADVVARLERDHGFPVSTEAAGRIAMLMPGLKDRARTLAELAESALFVVREAPLPMTEKAEALLTEAARDDLAALLLDFDK
TDFSKAALHEAMKAYAEREEKKLGAIAQPLRAALTGSTVSPPIDAVMEALGPIEVRKRIFSVLGE

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=502, Percent_Identity=36.0557768924303, Blast_Score=261, Evalue=1e-69,
Organism=Escherichia coli, GI1788743, Length=472, Percent_Identity=45.3389830508475, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI226510925, Length=241, Percent_Identity=36.0995850622407, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71993394, Length=479, Percent_Identity=33.4029227557411, Blast_Score=232, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI17511007, Length=278, Percent_Identity=22.6618705035971, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=487, Percent_Identity=32.0328542094456, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6324742, Length=111, Percent_Identity=35.1351351351351, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321192, Length=231, Percent_Identity=29.004329004329, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665321, Length=477, Percent_Identity=34.5911949685535, Blast_Score=253, Evalue=3e-67,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_ACICJ (A5FZX8)

Other databases:

- EMBL:   CP000697
- RefSeq:   YP_001235079.1
- ProteinModelPortal:   A5FZX8
- SMR:   A5FZX8
- STRING:   A5FZX8
- GeneID:   5160851
- GenomeReviews:   CP000697_GR
- KEGG:   acr:Acry_1959
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   ACRY349163:ACRY_1959-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51935; Mature: 51804

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 241-241

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRVRFAPSPTGMLHIGGARTALFNYLFARHHGGQFLLRVEDTDRERSTPEATKVILDA
CEEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHH
LDWLDLKPDEPPVYQSTRYARHREVAEQMLAAGQAYRCYCSREELAEMRAEAERSKKPFR
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCC
YDGRWRDRDPAEAPAGVDPVIRLRAPREGETVIHDLVQGEVRVKNAELDDMILLRSDGTP
CCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCEEEECCCCCCEEEEECCCCC
TYLHAVVVDDHDMGITHVIRGDDHLTNTFRQAQIYDAMGWARPNFAHIPLIHGADGAKLS
EEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHH
KRHGAVSVLQFRDEGYLPEALCNYLLRLGWGHGDAEILPREEQVALFDLDGVGRAASRMD
HHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHH
YAKLLHVNAVFLRAAEDERLAADVVARLERDHGFPVSTEAAGRIAMLMPGLKDRARTLAE
HHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHH
LAESALFVVREAPLPMTEKAEALLTEAARDDLAALLLDFDKTDFSKAALHEAMKAYAERE
HHHHHEEEHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
EKKLGAIAQPLRAALTGSTVSPPIDAVMEALGPIEVRKRIFSVLGE
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIRVRFAPSPTGMLHIGGARTALFNYLFARHHGGQFLLRVEDTDRERSTPEATKVILDA
EEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHH
LDWLDLKPDEPPVYQSTRYARHREVAEQMLAAGQAYRCYCSREELAEMRAEAERSKKPFR
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCC
YDGRWRDRDPAEAPAGVDPVIRLRAPREGETVIHDLVQGEVRVKNAELDDMILLRSDGTP
CCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCEEEECCCCCCEEEEECCCCC
TYLHAVVVDDHDMGITHVIRGDDHLTNTFRQAQIYDAMGWARPNFAHIPLIHGADGAKLS
EEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHH
KRHGAVSVLQFRDEGYLPEALCNYLLRLGWGHGDAEILPREEQVALFDLDGVGRAASRMD
HHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHH
YAKLLHVNAVFLRAAEDERLAADVVARLERDHGFPVSTEAAGRIAMLMPGLKDRARTLAE
HHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHH
LAESALFVVREAPLPMTEKAEALLTEAARDDLAALLLDFDKTDFSKAALHEAMKAYAERE
HHHHHEEEHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
EKKLGAIAQPLRAALTGSTVSPPIDAVMEALGPIEVRKRIFSVLGE
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA