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The map label for this gene is 148260869

Identifier: 148260869

GI number: 148260869

Start: 2067395

End: 2068771

Strand: Reverse

Name: 148260869

Synonym: Acry_1875

Alternate gene names: NA

Gene position: 2068771-2067395 (Counterclockwise)

Preceding gene: 148260870

Following gene: 148260868

Centisome position: 61.04

GC content: 71.17

Gene sequence:

>1377_bases
TTGTCCAACCCCGGAGAGGCCGACGCCACCCCGATCGATCCCGCGCAGCCCGCCGCGCGCCAACTCGCCGACTGGTTTGC
CGCCGGCTGCAAGCCGCCGGCCGATTTCCGCATCGGCACCGAACACGAAAGCTTCGGCTTCCGCCGCGACAACTTCGCGC
CGCCGCCCTACGCGCCGGACGGCATCCGCGCCGTGCTCGAATCGGTCGCCGCCGCCGAGGGCATCGAGCCCATCCTCGAT
GCCGGCAACCCGATCGGCCTGAAGGGCAGGGGGTTCTCGATCTCCCTCGAGCCCGGCGGCCAGTTCGAGCTCTCCGGCGC
CCCGCTCGCCTCGATCCACGAGACGAAGCGGGAGATCGACGCCCATATCGCCCGCGTCCACCGCATCGGCGAGGCGCTCG
GCCTCGGCTTCGCCCCGCTCGGCTTCCACCCGACGGCCCGCCGCGCCGATTTCGACTGGATGCCCAAGGGCCGCTACGCC
ATCATGCGCGCCTACATGCAGAAGGTCGGCACCCGCGGGCTCGACATGATGCTGCGCACCTGCACCGTCCAGGCCAATCT
CGACTTCGCCTCCGAGGCCGACATGGTGCGGAAGATGCGCGTCGGCCTCGCCCTGCAGCCGCTCGCCACCGCCCTGTTCG
CCAACGGCCCCTTCGTCGAGGGCAGGCCGAACGGCCTGCTCTCCAACCGCGCCGATGCCTGGCTCGATACCGACAACGCC
CGCGCCGGCATCCCCCGCATGGTCTTCGAGGACGGTTTCGGCTTCGAGCGCTACGCCGACTACATGCTCGATATCCCGAT
GTATTTCGTCTACCGCGACGGGCGGTACATCGACGTCTCCGGCAAGTCCTTCCGCGACTTCATCGCCGGCCGGCTCGAGG
GCCTCGCCGCCGAGCCCGCCACGATCGGCGACTTCGCCGACCACACCACCACCACCTTCCCCGATGTCCGCCTCAAGCGC
TTCATCGAGACCCGCGGCGCCGATTCCGGCAATCCCGCCATGCTCCTCGCCCAGCCCGCCCTCTGGACCGGCCTGTTCTA
CGACGATGCGGCGCTCGACGCCGCCTCCGCCCTGATCCGCGACCTCTCCTGGGAGCAGATCGTCCACCTGCGCGATGAGG
TGCCCCGCCACGGGCTCGACATCGCGCTGCCCACCGGCACCCTGCGCGACCTCGCGCGCGACGTCCTCGCCATCGCCGCG
GACGGGCTGAAATCCCGCGCGCGGACCAACGCGGCCGGCGAGGACGAGCGCATCTACCTCGCCCCCCTCGAAGCCATCGC
CCATGGCGCGCCCACCCAGGCCGAGCACTGGCTCGGCCGCTACCACGGCGCCTGGCACGGCGACGCCACGCGCATCTTCG
CCGAAGCCGCCCTCTGA

Upstream 100 bases:

>100_bases
AACCCTCGGACCGCGCATCCTGCGCGCCGAAACCGCCGCCATCGCCGGGCTCGCGCGTCTGCTCGCGGGGTCCGCGCCCA
GCTGATTTCAGGAGCCCGCT

Downstream 100 bases:

>100_bases
GCCTGTCCGGCCCGGGCCGCCGTCGACGCCCTCACCACGGATTGCGTTGACGCGCGGCCCCGCTCCGCTACACCCCGCGG
CATGACCGCATCCTCCCGCC

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSNPGEADATPIDPAQPAARQLADWFAAGCKPPADFRIGTEHESFGFRRDNFAPPPYAPDGIRAVLESVAAAEGIEPILD
AGNPIGLKGRGFSISLEPGGQFELSGAPLASIHETKREIDAHIARVHRIGEALGLGFAPLGFHPTARRADFDWMPKGRYA
IMRAYMQKVGTRGLDMMLRTCTVQANLDFASEADMVRKMRVGLALQPLATALFANGPFVEGRPNGLLSNRADAWLDTDNA
RAGIPRMVFEDGFGFERYADYMLDIPMYFVYRDGRYIDVSGKSFRDFIAGRLEGLAAEPATIGDFADHTTTTFPDVRLKR
FIETRGADSGNPAMLLAQPALWTGLFYDDAALDAASALIRDLSWEQIVHLRDEVPRHGLDIALPTGTLRDLARDVLAIAA
DGLKSRARTNAAGEDERIYLAPLEAIAHGAPTQAEHWLGRYHGAWHGDATRIFAEAAL

Sequences:

>Translated_458_residues
MSNPGEADATPIDPAQPAARQLADWFAAGCKPPADFRIGTEHESFGFRRDNFAPPPYAPDGIRAVLESVAAAEGIEPILD
AGNPIGLKGRGFSISLEPGGQFELSGAPLASIHETKREIDAHIARVHRIGEALGLGFAPLGFHPTARRADFDWMPKGRYA
IMRAYMQKVGTRGLDMMLRTCTVQANLDFASEADMVRKMRVGLALQPLATALFANGPFVEGRPNGLLSNRADAWLDTDNA
RAGIPRMVFEDGFGFERYADYMLDIPMYFVYRDGRYIDVSGKSFRDFIAGRLEGLAAEPATIGDFADHTTTTFPDVRLKR
FIETRGADSGNPAMLLAQPALWTGLFYDDAALDAASALIRDLSWEQIVHLRDEVPRHGLDIALPTGTLRDLARDVLAIAA
DGLKSRARTNAAGEDERIYLAPLEAIAHGAPTQAEHWLGRYHGAWHGDATRIFAEAAL
>Mature_457_residues
SNPGEADATPIDPAQPAARQLADWFAAGCKPPADFRIGTEHESFGFRRDNFAPPPYAPDGIRAVLESVAAAEGIEPILDA
GNPIGLKGRGFSISLEPGGQFELSGAPLASIHETKREIDAHIARVHRIGEALGLGFAPLGFHPTARRADFDWMPKGRYAI
MRAYMQKVGTRGLDMMLRTCTVQANLDFASEADMVRKMRVGLALQPLATALFANGPFVEGRPNGLLSNRADAWLDTDNAR
AGIPRMVFEDGFGFERYADYMLDIPMYFVYRDGRYIDVSGKSFRDFIAGRLEGLAAEPATIGDFADHTTTTFPDVRLKRF
IETRGADSGNPAMLLAQPALWTGLFYDDAALDAASALIRDLSWEQIVHLRDEVPRHGLDIALPTGTLRDLARDVLAIAAD
GLKSRARTNAAGEDERIYLAPLEAIAHGAPTQAEHWLGRYHGAWHGDATRIFAEAAL

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 49941; Mature: 49810

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPGEADATPIDPAQPAARQLADWFAAGCKPPADFRIGTEHESFGFRRDNFAPPPYAPD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCH
GIRAVLESVAAAEGIEPILDAGNPIGLKGRGFSISLEPGGQFELSGAPLASIHETKREID
HHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHH
AHIARVHRIGEALGLGFAPLGFHPTARRADFDWMPKGRYAIMRAYMQKVGTRGLDMMLRT
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
CTVQANLDFASEADMVRKMRVGLALQPLATALFANGPFVEGRPNGLLSNRADAWLDTDNA
HEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
RAGIPRMVFEDGFGFERYADYMLDIPMYFVYRDGRYIDVSGKSFRDFIAGRLEGLAAEPA
CCCCCHHHHHCCCCHHHHHHHHHCCCEEEEEECCEEEECCCCHHHHHHHHHHHCCCCCCC
TIGDFADHTTTTFPDVRLKRFIETRGADSGNPAMLLAQPALWTGLFYDDAALDAASALIR
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCHHHHCCEECHHHHHHHHHHHH
DLSWEQIVHLRDEVPRHGLDIALPTGTLRDLARDVLAIAADGLKSRARTNAAGEDERIYL
HCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
APLEAIAHGAPTQAEHWLGRYHGAWHGDATRIFAEAAL
EEHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SNPGEADATPIDPAQPAARQLADWFAAGCKPPADFRIGTEHESFGFRRDNFAPPPYAPD
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCH
GIRAVLESVAAAEGIEPILDAGNPIGLKGRGFSISLEPGGQFELSGAPLASIHETKREID
HHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHH
AHIARVHRIGEALGLGFAPLGFHPTARRADFDWMPKGRYAIMRAYMQKVGTRGLDMMLRT
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
CTVQANLDFASEADMVRKMRVGLALQPLATALFANGPFVEGRPNGLLSNRADAWLDTDNA
HEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
RAGIPRMVFEDGFGFERYADYMLDIPMYFVYRDGRYIDVSGKSFRDFIAGRLEGLAAEPA
CCCCCHHHHHCCCCHHHHHHHHHCCCEEEEEECCEEEECCCCHHHHHHHHHHHCCCCCCC
TIGDFADHTTTTFPDVRLKRFIETRGADSGNPAMLLAQPALWTGLFYDDAALDAASALIR
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCHHHHCCEECHHHHHHHHHHHH
DLSWEQIVHLRDEVPRHGLDIALPTGTLRDLARDVLAIAADGLKSRARTNAAGEDERIYL
HCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
APLEAIAHGAPTQAEHWLGRYHGAWHGDATRIFAEAAL
EEHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA