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The map label for this gene is livK [C]

Identifier: 148260860

GI number: 148260860

Start: 2058486

End: 2059607

Strand: Reverse

Name: livK [C]

Synonym: Acry_1866

Alternate gene names: 148260860

Gene position: 2059607-2058486 (Counterclockwise)

Preceding gene: 148260861

Following gene: 148260859

Centisome position: 60.77

GC content: 62.57

Gene sequence:

>1122_bases
ATGGTCAGAAAACTCCTTTTTGGCGCCGGCATGATTGCGCTGTCGTGCGGGCTGGGTGTCGCGCACGCGGCCTCGGGCAC
GATCGAGATCGGCATGATCGCGGACATGACCGGACCGGCGGCGCTCAGCGGGCAGCACAAGGTCGATGGCGCGAAGCTCG
CCGTTGACGAGATCAACGCCCATGGCGGCATCAACGGCAAGAAGATCAAGCTGATCGTCGAGGACGATCGCGGGCAGAAC
CAGGCCGGCGTCTCGGCCTATCAGAAACTTGCGGCGAACCAGAATATCGTCGCGATCATCGGCTCGATCCGCAGCACCAT
CGTGCAGGCGACGCTGCCTTACGTCGCGCGCTACAAGATTCCGACCATGATCGGCGGCACCGATCCGAAGCTGACGCATG
TGGGCAACCAGTGGGTGTTCCGCTTCCGCCCGAACGATACCTATGCCTCGGCGACGATGGCCCAGTATGCGGTAAAGACG
ATGGGCAGCAAGAAGGTTGCCATTCTTTACGACACCGACGCCTTCGGCTCGGCCGGCAATCAGCTGCTCAAGGCGGCGCT
CGTGAAGGACGGCGCGAAAGTGGTCAGCGACCAGGGCTACACGACCGCGACGCGCGACTACACCTCGTTCCTTGAAAAGA
TCAAGAACTCCGGCGCGGATACGCTGGCCACCTACATGACGAATTCGGAGGACGAGGCGCAGATGCTCAAGCAGCTGCGC
CAGCTCGACCTCAAGGTGAAGATCATCGGGTCCACCTCGATCGCGACGGCGGTGTGCATCCAGCTTGCCGGGTCGGCGGT
CGACGGCACCTACGGCGTTTCCGATTTCGTGGCGGACGGCAATGACGCGGCGAAGGCGTTCACCAAGCTCTATGTCGCGA
AATATCACAGCCAGCCCGACGTGTTCGGCGGCTGGGTGTATGACGCGATGAACGTCCTGAGCCGGGTGATCGCGAAGGAC
GGCACCGCGCCGGCGGCGATCCAGAAGGGCATTCGCGCGGTGAAGGGCTATCACGGCGTCGAGGGCACCTACAATTTCGA
CGCGAACGGCGACGGGCTGCACGGCTACTGGGTGGTGAAGATGCAGCACCAGAAGGTCATCCCGATCAAATACGTCGGCT
GA

Upstream 100 bases:

>100_bases
CCCGGCGCCGGCGTGACACGGCGGGCCGTGACCGCGACGGAAGTGGCTTCAGGCCCGGATCGGTCCGGAAAATTCACAAA
CACTAGGGGAAACAAATCCA

Downstream 100 bases:

>100_bases
AGGCCGCGCGCCGTCCGGGCAGGGTCGTGGCCCGGACGGCGTTCGCCGTTATCGTGACGGAATGAAAAACCAGCTGCCCG
GGATATCGTCTGCGTGTTCT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MVRKLLFGAGMIALSCGLGVAHAASGTIEIGMIADMTGPAALSGQHKVDGAKLAVDEINAHGGINGKKIKLIVEDDRGQN
QAGVSAYQKLAANQNIVAIIGSIRSTIVQATLPYVARYKIPTMIGGTDPKLTHVGNQWVFRFRPNDTYASATMAQYAVKT
MGSKKVAILYDTDAFGSAGNQLLKAALVKDGAKVVSDQGYTTATRDYTSFLEKIKNSGADTLATYMTNSEDEAQMLKQLR
QLDLKVKIIGSTSIATAVCIQLAGSAVDGTYGVSDFVADGNDAAKAFTKLYVAKYHSQPDVFGGWVYDAMNVLSRVIAKD
GTAPAAIQKGIRAVKGYHGVEGTYNFDANGDGLHGYWVVKMQHQKVIPIKYVG

Sequences:

>Translated_373_residues
MVRKLLFGAGMIALSCGLGVAHAASGTIEIGMIADMTGPAALSGQHKVDGAKLAVDEINAHGGINGKKIKLIVEDDRGQN
QAGVSAYQKLAANQNIVAIIGSIRSTIVQATLPYVARYKIPTMIGGTDPKLTHVGNQWVFRFRPNDTYASATMAQYAVKT
MGSKKVAILYDTDAFGSAGNQLLKAALVKDGAKVVSDQGYTTATRDYTSFLEKIKNSGADTLATYMTNSEDEAQMLKQLR
QLDLKVKIIGSTSIATAVCIQLAGSAVDGTYGVSDFVADGNDAAKAFTKLYVAKYHSQPDVFGGWVYDAMNVLSRVIAKD
GTAPAAIQKGIRAVKGYHGVEGTYNFDANGDGLHGYWVVKMQHQKVIPIKYVG
>Mature_373_residues
MVRKLLFGAGMIALSCGLGVAHAASGTIEIGMIADMTGPAALSGQHKVDGAKLAVDEINAHGGINGKKIKLIVEDDRGQN
QAGVSAYQKLAANQNIVAIIGSIRSTIVQATLPYVARYKIPTMIGGTDPKLTHVGNQWVFRFRPNDTYASATMAQYAVKT
MGSKKVAILYDTDAFGSAGNQLLKAALVKDGAKVVSDQGYTTATRDYTSFLEKIKNSGADTLATYMTNSEDEAQMLKQLR
QLDLKVKIIGSTSIATAVCIQLAGSAVDGTYGVSDFVADGNDAAKAFTKLYVAKYHSQPDVFGGWVYDAMNVLSRVIAKD
GTAPAAIQKGIRAVKGYHGVEGTYNFDANGDGLHGYWVVKMQHQKVIPIKYVG

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=354, Percent_Identity=28.8135593220339, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI48994941, Length=349, Percent_Identity=26.9340974212034, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39653; Mature: 39653

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRKLLFGAGMIALSCGLGVAHAASGTIEIGMIADMTGPAALSGQHKVDGAKLAVDEINA
CCHHHHHHCCHHHHHHCCCHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHCC
HGGINGKKIKLIVEDDRGQNQAGVSAYQKLAANQNIVAIIGSIRSTIVQATLPYVARYKI
CCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCC
PTMIGGTDPKLTHVGNQWVFRFRPNDTYASATMAQYAVKTMGSKKVAILYDTDAFGSAGN
CEEECCCCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHH
QLLKAALVKDGAKVVSDQGYTTATRDYTSFLEKIKNSGADTLATYMTNSEDEAQMLKQLR
HHHHHHHHHCCHHHHCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHH
QLDLKVKIIGSTSIATAVCIQLAGSAVDGTYGVSDFVADGNDAAKAFTKLYVAKYHSQPD
HCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCC
VFGGWVYDAMNVLSRVIAKDGTAPAAIQKGIRAVKGYHGVEGTYNFDANGDGLHGYWVVK
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEEEE
MQHQKVIPIKYVG
EECCEECEEEECC
>Mature Secondary Structure
MVRKLLFGAGMIALSCGLGVAHAASGTIEIGMIADMTGPAALSGQHKVDGAKLAVDEINA
CCHHHHHHCCHHHHHHCCCHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHCC
HGGINGKKIKLIVEDDRGQNQAGVSAYQKLAANQNIVAIIGSIRSTIVQATLPYVARYKI
CCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCC
PTMIGGTDPKLTHVGNQWVFRFRPNDTYASATMAQYAVKTMGSKKVAILYDTDAFGSAGN
CEEECCCCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHH
QLLKAALVKDGAKVVSDQGYTTATRDYTSFLEKIKNSGADTLATYMTNSEDEAQMLKQLR
HHHHHHHHHCCHHHHCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHH
QLDLKVKIIGSTSIATAVCIQLAGSAVDGTYGVSDFVADGNDAAKAFTKLYVAKYHSQPD
HCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCC
VFGGWVYDAMNVLSRVIAKDGTAPAAIQKGIRAVKGYHGVEGTYNFDANGDGLHGYWVVK
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEEEE
MQHQKVIPIKYVG
EECCEECEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]