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The map label for this gene is pcaF [H]

Identifier: 148260841

GI number: 148260841

Start: 2037125

End: 2038327

Strand: Reverse

Name: pcaF [H]

Synonym: Acry_1847

Alternate gene names: 148260841

Gene position: 2038327-2037125 (Counterclockwise)

Preceding gene: 148260842

Following gene: 148260840

Centisome position: 60.14

GC content: 73.23

Gene sequence:

>1203_bases
ATGACCGAGGCCTTTATTTGCGAGTATGTGCGGACGCCGTTTGGGCGTTATGGCGGGACGCTTTCGGCGGTTCGCGCGGA
CGATCTGGCGGCGGTGGCGATCCGCGGGGTGATGGCGCGGGCGGCGGGGCTCGATCCGGCGGCGGTGGACGAGGTGCTGT
TCGGCTGTGCGAACCAGGCGGGCGAGGATAACCGGAACGTGGCGCGGATGGCGCTGCTGCTGGCGGGGCTGCCGGAGAGC
GTGCCGGGGGCGACGGTCAACCGGCTGTGCGGCTCCGGGCTGGATGCGGTGGGGAGTGCGGCGCGGGCGATCCGGCTGGG
CGAGGCGGGGGTGGCGATCGCCGGCGGGGTGGAGAGCATGACCCGCGCGCCCTTCGTGATGCCGAAGGCGGGGGCGGCGT
TCGACCGGTCGAACGCGGTGTATGACACGACGATCGGCTGGCGCTTCGTCAACCCGCTGATGAAGGCGCAATACGGCGTC
GACGCGATGCCGGAGACGGCGGAGAACGTCGCCGCCGATTTCAACATCTCGCGGGCGGACCAGGACGGGTTCGCGCTGGC
GAGCCAGCGCAAGTGCCAGGCGGCGGCAGAGGCCGGCTTCTTCGCCGGCGAGATCACCCCGGTGACCATTCGCGGCCGCA
AGGGCGACACGGTGGTTGACACCGACGAGCATCCGCGGCCGGAGACGACGCTGGAGGGGCTGGCGAGGCTGAGCACGCCG
TTCCGCGAGCCCGGCACGGTGACGGCGGGCAATGCGTCGGGGGTGAATGACGGGGCGGCGGCGCTGATCCTCGCCTCGGC
GGCGGCGGCGCAGGCGCATGGGCTGACGCCGCGCGGGCGCGTGGTCGGCATGGCGACGGCGGGTGTGCCGCCGCGGATCA
TGGGCATGGGGCCGGCGCCGGCGGTGAGGAAGCTGCTGGCGCGGACCGGGGTTTCGCTGGACGCGGTGGACGTGATCGAG
CTGAACGAGGCCTTCGCGGCGCAGGCGCTGGCGGTGACGCGGGATCTCGGGCTTGCGGATGACGACCCGCGGGTCAACCC
GCATGGCGGGGCGATCGCGCTGGGGCATCCGCTGGGCGCGTCGGGGGCGCGGCTGGCGGGCACGGCACTGCGGGCGCTGG
AAGCGCGGGGCGGCAGGCTGGCGATCGCGACGATGTGCATCGGTGTCGGCCAGGGCATCGCAGTGCTGATCGAGCGGGTG
TGA

Upstream 100 bases:

>100_bases
CGGATGAGACCTCGCCCGGCGTCTGGCGGCGCGACATCGTGCTGCAGGGCGAGGGCGAAACGGTATTTCTGGACTTTTGA
ACAAGGGAAGCAGCGTATCC

Downstream 100 bases:

>100_bases
GGCGATGATCGACAAGTTCGTGGCCTCGGTCGAGGCGGCGCTGGAGGGGATCGGCGATGGCGCCGCGATCATGGTGGGCG
GGTTCGGCGCGGTGGGGCAG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTEAFICEYVRTPFGRYGGTLSAVRADDLAAVAIRGVMARAAGLDPAAVDEVLFGCANQAGEDNRNVARMALLLAGLPES
VPGATVNRLCGSGLDAVGSAARAIRLGEAGVAIAGGVESMTRAPFVMPKAGAAFDRSNAVYDTTIGWRFVNPLMKAQYGV
DAMPETAENVAADFNISRADQDGFALASQRKCQAAAEAGFFAGEITPVTIRGRKGDTVVDTDEHPRPETTLEGLARLSTP
FREPGTVTAGNASGVNDGAAALILASAAAAQAHGLTPRGRVVGMATAGVPPRIMGMGPAPAVRKLLARTGVSLDAVDVIE
LNEAFAAQALAVTRDLGLADDDPRVNPHGGAIALGHPLGASGARLAGTALRALEARGGRLAIATMCIGVGQGIAVLIERV

Sequences:

>Translated_400_residues
MTEAFICEYVRTPFGRYGGTLSAVRADDLAAVAIRGVMARAAGLDPAAVDEVLFGCANQAGEDNRNVARMALLLAGLPES
VPGATVNRLCGSGLDAVGSAARAIRLGEAGVAIAGGVESMTRAPFVMPKAGAAFDRSNAVYDTTIGWRFVNPLMKAQYGV
DAMPETAENVAADFNISRADQDGFALASQRKCQAAAEAGFFAGEITPVTIRGRKGDTVVDTDEHPRPETTLEGLARLSTP
FREPGTVTAGNASGVNDGAAALILASAAAAQAHGLTPRGRVVGMATAGVPPRIMGMGPAPAVRKLLARTGVSLDAVDVIE
LNEAFAAQALAVTRDLGLADDDPRVNPHGGAIALGHPLGASGARLAGTALRALEARGGRLAIATMCIGVGQGIAVLIERV
>Mature_399_residues
TEAFICEYVRTPFGRYGGTLSAVRADDLAAVAIRGVMARAAGLDPAAVDEVLFGCANQAGEDNRNVARMALLLAGLPESV
PGATVNRLCGSGLDAVGSAARAIRLGEAGVAIAGGVESMTRAPFVMPKAGAAFDRSNAVYDTTIGWRFVNPLMKAQYGVD
AMPETAENVAADFNISRADQDGFALASQRKCQAAAEAGFFAGEITPVTIRGRKGDTVVDTDEHPRPETTLEGLARLSTPF
REPGTVTAGNASGVNDGAAALILASAAAAQAHGLTPRGRVVGMATAGVPPRIMGMGPAPAVRKLLARTGVSLDAVDVIEL
NEAFAAQALAVTRDLGLADDDPRVNPHGGAIALGHPLGASGARLAGTALRALEARGGRLAIATMCIGVGQGIAVLIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=399, Percent_Identity=42.6065162907268, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=41.3098236775819, Blast_Score=278, Evalue=5e-75,
Organism=Homo sapiens, GI4501853, Length=398, Percent_Identity=41.4572864321608, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI4557237, Length=407, Percent_Identity=36.3636363636364, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI4504327, Length=421, Percent_Identity=30.6413301662708, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI194353979, Length=391, Percent_Identity=30.690537084399, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=65.5860349127182, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI1788554, Length=402, Percent_Identity=48.7562189054726, Blast_Score=315, Evalue=2e-87,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=44.3890274314214, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI48994986, Length=409, Percent_Identity=43.520782396088, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1788683, Length=408, Percent_Identity=29.4117647058824, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI133906874, Length=402, Percent_Identity=42.2885572139304, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI25147385, Length=404, Percent_Identity=36.1386138613861, Blast_Score=230, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI17535921, Length=406, Percent_Identity=32.2660098522167, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=32.093023255814, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17535917, Length=402, Percent_Identity=31.592039800995, Blast_Score=165, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6325229, Length=403, Percent_Identity=39.9503722084367, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6322031, Length=341, Percent_Identity=40.7624633431085, Blast_Score=229, Evalue=7e-61,
Organism=Drosophila melanogaster, GI24655093, Length=400, Percent_Identity=45.75, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI17648125, Length=404, Percent_Identity=40.8415841584158, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24640423, Length=406, Percent_Identity=36.9458128078818, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI17137578, Length=421, Percent_Identity=31.8289786223278, Blast_Score=169, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 40773; Mature: 40642

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAFICEYVRTPFGRYGGTLSAVRADDLAAVAIRGVMARAAGLDPAAVDEVLFGCANQA
CCHHHHHHHHHCCHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
GEDNRNVARMALLLAGLPESVPGATVNRLCGSGLDAVGSAARAIRLGEAGVAIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHEEECCCCEEEECCHHHH
TRAPFVMPKAGAAFDRSNAVYDTTIGWRFVNPLMKAQYGVDAMPETAENVAADFNISRAD
HCCCEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCC
QDGFALASQRKCQAAAEAGFFAGEITPVTIRGRKGDTVVDTDEHPRPETTLEGLARLSTP
CCCHHHHHHHHHHHHHHCCCEECCEEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHCCC
FREPGTVTAGNASGVNDGAAALILASAAAAQAHGLTPRGRVVGMATAGVPPRIMGMGPAP
CCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEECCCCCH
AVRKLLARTGVSLDAVDVIELNEAFAAQALAVTRDLGLADDDPRVNPHGGAIALGHPLGA
HHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCC
SGARLAGTALRALEARGGRLAIATMCIGVGQGIAVLIERV
CCCHHHHHHHHHHHHCCCEEEEEHHHHHCCCCHHHHEECC
>Mature Secondary Structure 
TEAFICEYVRTPFGRYGGTLSAVRADDLAAVAIRGVMARAAGLDPAAVDEVLFGCANQA
CHHHHHHHHHCCHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
GEDNRNVARMALLLAGLPESVPGATVNRLCGSGLDAVGSAARAIRLGEAGVAIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHEEECCCCEEEECCHHHH
TRAPFVMPKAGAAFDRSNAVYDTTIGWRFVNPLMKAQYGVDAMPETAENVAADFNISRAD
HCCCEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCC
QDGFALASQRKCQAAAEAGFFAGEITPVTIRGRKGDTVVDTDEHPRPETTLEGLARLSTP
CCCHHHHHHHHHHHHHHCCCEECCEEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHCCC
FREPGTVTAGNASGVNDGAAALILASAAAAQAHGLTPRGRVVGMATAGVPPRIMGMGPAP
CCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEECCCCCH
AVRKLLARTGVSLDAVDVIELNEAFAAQALAVTRDLGLADDDPRVNPHGGAIALGHPLGA
HHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCC
SGARLAGTALRALEARGGRLAIATMCIGVGQGIAVLIERV
CCCHHHHHHHHHHHHCCCEEEEEHHHHHCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]