Click here to switch to the map view.

The map label for this gene is nifS [H]

Identifier: 148260761

GI number: 148260761

Start: 1948546

End: 1949688

Strand: Reverse

Name: nifS [H]

Synonym: Acry_1766

Alternate gene names: 148260761

Gene position: 1949688-1948546 (Counterclockwise)

Preceding gene: 148260762

Following gene: 148260760

Centisome position: 57.53

GC content: 75.85

Gene sequence:

>1143_bases
TTGCCCGGGGCTGAGCCGGGCCTCTACCTCGACGCCAACGCGACCGAGGCGCTGCGCCCCGAAGCCCGCGAGGCGGTGCT
CGCCGCCTTCGATCTCGTCGGCAATCCGGCCTCCGTCCACGCCGATGGCAGGGCCGCGCGCAAGCTGCTCGAATCCGCCC
GCGCCGCCCTTGCCGCACGCCTCGGCGCCCGGCCGGAAGACGTCGTCTTCTGCGCGGGCGCGACCGAGGCGAACGCGATG
GCGATCCACGGCCTCGCGGGCGGCGGCGCCATCGTGATCGGCGCCACCGAGCACGATGCCGTGCGCGCCGCCGCCCCCGC
CGCCCTCATCGCCCCGGTCGATGCCGCCGGCCGGCTCGACCAGGCCGGCTTCGCCGCCCTGCTGGCCGCGCACCGCCCGG
CACTCGCCTGCGTCATGGCGGCGAACAACGAAACTGGCGTGCTGAACGACATCGCCGCCATCGCCGCCCTCTGCGCCGCG
CAGGGCACGCGCCTGCATGTCGATGCCGTGCAGGCCGCCGGCCGCGTCGCCGATCCCGATTATCTCGCCCAGGGGGCGTC
CAGCGTCGCGATCTCCGCGCACAAGCTCGGCGGCCCGAAAGGGGTGGGCGCCCTGCTGCTCGCGCCCGAGGCCGCGTCCG
CCTTCGCGCCGCTGATCCGCGGCGGCGGGCAGGAGCGCGGCCGCCGCGGCGGCACGCACAACCTGCCCGGCATCGCCGGC
TTCGCCGCCGCCCTCGACGCCGCCCACGCAACCACATGCCCCGATCCGCGCCTCGCCGCCCTGCGCGATGCGATCGAGGC
CGGCGCGCGCGACTGCGGCGCCCGCATCGCCGGCGCCGGGGCCCCGCGTTTGCCGAACACGGCCTGCCTCGTCCTGCCCG
GCCTTGCCGCCGAAGCCCAGGTCATCGCGCTCGATCTCGACGGCATCCGCGTTTCCGCCGGCTCGGCCTGCTCCTCCGGC
AAGGTCGCCAGCAGCCACGTTCTCGCCGCCATGGGCCTCGGCGATCTCGCCGGCAACGCCATCCGCGTCTCGTTGCCCTG
GAACGCGCCGGACGACGCGCCCGCCCGTTTCCTCGCCGCATATCGGCGTCTTGCCGCCCGCGCCTTGCGCAAATCCGCGC
ATGTCTCTACCTCGGCAGCGTGA

Upstream 100 bases:

>100_bases
CGGCGCGAAACGGAGCATGCCGAACGGCGCGAAACGGAACATGCCGAGCGGCGCGAACCGGATCGTGCCGGGCCGCGCGA
AACCGACCATGACGAGCCCG

Downstream 100 bases:

>100_bases
ACGGCGGCGGAACGGCGCGCGCCTTCCACCACGCAACGGTTTCAAGGGTTTCAAATGCCGAACATGACTTTCATCGAGCG
CGACGGCACGCGCCGGACGG

Product: class V aminotransferase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein NifS [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MPGAEPGLYLDANATEALRPEAREAVLAAFDLVGNPASVHADGRAARKLLESARAALAARLGARPEDVVFCAGATEANAM
AIHGLAGGGAIVIGATEHDAVRAAAPAALIAPVDAAGRLDQAGFAALLAAHRPALACVMAANNETGVLNDIAAIAALCAA
QGTRLHVDAVQAAGRVADPDYLAQGASSVAISAHKLGGPKGVGALLLAPEAASAFAPLIRGGGQERGRRGGTHNLPGIAG
FAAALDAAHATTCPDPRLAALRDAIEAGARDCGARIAGAGAPRLPNTACLVLPGLAAEAQVIALDLDGIRVSAGSACSSG
KVASSHVLAAMGLGDLAGNAIRVSLPWNAPDDAPARFLAAYRRLAARALRKSAHVSTSAA

Sequences:

>Translated_380_residues
MPGAEPGLYLDANATEALRPEAREAVLAAFDLVGNPASVHADGRAARKLLESARAALAARLGARPEDVVFCAGATEANAM
AIHGLAGGGAIVIGATEHDAVRAAAPAALIAPVDAAGRLDQAGFAALLAAHRPALACVMAANNETGVLNDIAAIAALCAA
QGTRLHVDAVQAAGRVADPDYLAQGASSVAISAHKLGGPKGVGALLLAPEAASAFAPLIRGGGQERGRRGGTHNLPGIAG
FAAALDAAHATTCPDPRLAALRDAIEAGARDCGARIAGAGAPRLPNTACLVLPGLAAEAQVIALDLDGIRVSAGSACSSG
KVASSHVLAAMGLGDLAGNAIRVSLPWNAPDDAPARFLAAYRRLAARALRKSAHVSTSAA
>Mature_379_residues
PGAEPGLYLDANATEALRPEAREAVLAAFDLVGNPASVHADGRAARKLLESARAALAARLGARPEDVVFCAGATEANAMA
IHGLAGGGAIVIGATEHDAVRAAAPAALIAPVDAAGRLDQAGFAALLAAHRPALACVMAANNETGVLNDIAAIAALCAAQ
GTRLHVDAVQAAGRVADPDYLAQGASSVAISAHKLGGPKGVGALLLAPEAASAFAPLIRGGGQERGRRGGTHNLPGIAGF
AAALDAAHATTCPDPRLAALRDAIEAGARDCGARIAGAGAPRLPNTACLVLPGLAAEAQVIALDLDGIRVSAGSACSSGK
VASSHVLAAMGLGDLAGNAIRVSLPWNAPDDAPARFLAAYRRLAARALRKSAHVSTSAA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=356, Percent_Identity=32.8651685393258, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI156713448, Length=395, Percent_Identity=30.6329113924051, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI48994898, Length=364, Percent_Identity=35.1648351648352, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI25143064, Length=369, Percent_Identity=33.6043360433604, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17533177, Length=308, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319831, Length=369, Percent_Identity=33.3333333333333, Blast_Score=159, Evalue=5e-40,
Organism=Drosophila melanogaster, GI20129463, Length=360, Percent_Identity=32.7777777777778, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 37551; Mature: 37420

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGAEPGLYLDANATEALRPEAREAVLAAFDLVGNPASVHADGRAARKLLESARAALAAR
CCCCCCCEEEECCCCHHHCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHH
LGARPEDVVFCAGATEANAMAIHGLAGGGAIVIGATEHDAVRAAAPAALIAPVDAAGRLD
HCCCCCCEEEECCCCCCCEEEEEEECCCCEEEEECCCCCHHHHHCCCCEEECCCCCCCCC
QAGFAALLAAHRPALACVMAANNETGVLNDIAAIAALCAAQGTRLHVDAVQAAGRVADPD
HHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCCHH
YLAQGASSVAISAHKLGGPKGVGALLLAPEAASAFAPLIRGGGQERGRRGGTHNLPGIAG
HHHCCCCCEEEEHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHCCCCCCCCCCCHHH
FAAALDAAHATTCPDPRLAALRDAIEAGARDCGARIAGAGAPRLPNTACLVLPGLAAEAQ
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCE
VIALDLDGIRVSAGSACSSGKVASSHVLAAMGLGDLAGNAIRVSLPWNAPDDAPARFLAA
EEEEEECCEEEECCCCCCCCCCHHHHHHHHHCCHHHCCCEEEEEECCCCCCCHHHHHHHH
YRRLAARALRKSAHVSTSAA
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PGAEPGLYLDANATEALRPEAREAVLAAFDLVGNPASVHADGRAARKLLESARAALAAR
CCCCCCEEEECCCCHHHCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHH
LGARPEDVVFCAGATEANAMAIHGLAGGGAIVIGATEHDAVRAAAPAALIAPVDAAGRLD
HCCCCCCEEEECCCCCCCEEEEEEECCCCEEEEECCCCCHHHHHCCCCEEECCCCCCCCC
QAGFAALLAAHRPALACVMAANNETGVLNDIAAIAALCAAQGTRLHVDAVQAAGRVADPD
HHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCCHH
YLAQGASSVAISAHKLGGPKGVGALLLAPEAASAFAPLIRGGGQERGRRGGTHNLPGIAG
HHHCCCCCEEEEHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHCCCCCCCCCCCHHH
FAAALDAAHATTCPDPRLAALRDAIEAGARDCGARIAGAGAPRLPNTACLVLPGLAAEAQ
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCE
VIALDLDGIRVSAGSACSSGKVASSHVLAAMGLGDLAGNAIRVSLPWNAPDDAPARFLAA
EEEEEECCEEEECCCCCCCCCCHHHHHHHHHCCHHHCCCEEEEEECCCCCCCHHHHHHHH
YRRLAARALRKSAHVSTSAA
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11092875 [H]