BacMap

Definition	Acidiphilium cryptum JF-5 chromosome, complete genome.
Accession	NC_009484
Length	3,389,227

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The map label for this gene is paaG [H]

Identifier: 148260711

GI number: 148260711

Start: 1897687

End: 1898481

Strand: Reverse

Name: paaG [H]

Synonym: Acry_1715

Alternate gene names: 148260711

Gene position: 1898481-1897687 (Counterclockwise)

Preceding gene: 148260719

Following gene: 148260709

Centisome position: 56.02

GC content: 73.96

Gene sequence:

>795_bases
ATGGCGGCAGAGCTGATCGTGGAAAGCGTCGGAGCGGTGCGGGTGCTGAAGCTCAACCGGCCCGACCGTCTGAACGCGCT
GAACGAGGCGGTGCACGAGGCGCTCGCCGCCGCCTTCGCCGCCATCGAGGCGGACGATTCGGTCCGCGCCGCGCTGCTGA
CCGGCGAGGGCAGGGGGTTCTGCGCCGGCGCCGATCTCAACCAGTCCCTCGGCGGCGGCCCGCGCGATCTCGGCGCCTCG
ATCGACCGGCACTACAATCCGCTGGTCCGCCGCATGCGCGCCCTGCCGAAGCCGATCGTCGCCGCGGTCAACGGCGTCGC
CGCCGGCGCGGGCGCCAATCTCGCCCTCGCGGCGGATATCGTGCTCGCCTGCGAGTCGGCGAATTTCACCCAGGCCTTCA
TCCGCATCGGCCTGATCCCGGATGCCGGCGGCACCCATTTCCTCCCCCGCGCGGTGGGCGATGCCCGCGCCCGCGGCCTC
GCCATGCTGGGCGAGACCATCACCGCCCGCCAGGCCGCCGACTGGGGCCTGATCTGGCGCTGCCTGCCCGACGAGGGCTT
CCTCGCCGCCGCCCTCGCGGTCGCGGCCGATCTCGCCTCCCGCCCGACCCAGGCGCTCGCCGCGATGAAGGCGGCCTTCA
ACGCCGCCGGCGCCAACGGGCTCGACGCCCAGCTCGATCTCGAGCGCGACCTGCAACGCCGCATGGGCCGCACGCCGGAC
TTCGCCGAGGGTGTCGCCGCCTTCCGCGAGAAGCGCCCGGCGAACTTTACCGGCGCGCCGCCAGCGCGAGACTGA

Upstream 100 bases:

>100_bases
GAGATGCTCATTTCGTCTCCTTATTGCCGTAAGCGTCAGTTCTCGCCATACTCGGCGCACCGGCGCACACTAGTCGCCCG
GAACGCAGGGAGGAACAGGA

Downstream 100 bases:

>100_bases
GCGCGATCGCCCCGAGCCCCGCGAACAGCGCGCGGGGCCCGGCGCCCGCGTGCAGCAGCGCGGTCAGGATCAGGCTCGCC
GCGACCATCCCGGCGGCGGA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MAAELIVESVGAVRVLKLNRPDRLNALNEAVHEALAAAFAAIEADDSVRAALLTGEGRGFCAGADLNQSLGGGPRDLGAS
IDRHYNPLVRRMRALPKPIVAAVNGVAAGAGANLALAADIVLACESANFTQAFIRIGLIPDAGGTHFLPRAVGDARARGL
AMLGETITARQAADWGLIWRCLPDEGFLAAALAVAADLASRPTQALAAMKAAFNAAGANGLDAQLDLERDLQRRMGRTPD
FAEGVAAFREKRPANFTGAPPARD

Sequences:

>Translated_264_residues
MAAELIVESVGAVRVLKLNRPDRLNALNEAVHEALAAAFAAIEADDSVRAALLTGEGRGFCAGADLNQSLGGGPRDLGAS
IDRHYNPLVRRMRALPKPIVAAVNGVAAGAGANLALAADIVLACESANFTQAFIRIGLIPDAGGTHFLPRAVGDARARGL
AMLGETITARQAADWGLIWRCLPDEGFLAAALAVAADLASRPTQALAAMKAAFNAAGANGLDAQLDLERDLQRRMGRTPD
FAEGVAAFREKRPANFTGAPPARD
>Mature_263_residues
AAELIVESVGAVRVLKLNRPDRLNALNEAVHEALAAAFAAIEADDSVRAALLTGEGRGFCAGADLNQSLGGGPRDLGASI
DRHYNPLVRRMRALPKPIVAAVNGVAAGAGANLALAADIVLACESANFTQAFIRIGLIPDAGGTHFLPRAVGDARARGLA
MLGETITARQAADWGLIWRCLPDEGFLAAALAVAADLASRPTQALAAMKAAFNAAGANGLDAQLDLERDLQRRMGRTPDF
AEGVAAFREKRPANFTGAPPARD

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=246, Percent_Identity=38.2113821138211, Blast_Score=144, Evalue=6e-35,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=32.9457364341085, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI70995211, Length=253, Percent_Identity=28.4584980237154, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI45643119, Length=209, Percent_Identity=28.2296650717703, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI260275230, Length=209, Percent_Identity=28.2296650717703, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI260274832, Length=209, Percent_Identity=28.2296650717703, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI31542718, Length=248, Percent_Identity=25.4032258064516, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI25453481, Length=253, Percent_Identity=25.6916996047431, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI51538573, Length=253, Percent_Identity=25.6916996047431, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4757968, Length=253, Percent_Identity=25.6916996047431, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI49355787, Length=253, Percent_Identity=25.6916996047431, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI20127408, Length=172, Percent_Identity=29.6511627906977, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4757966, Length=208, Percent_Identity=28.3653846153846, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI51538581, Length=208, Percent_Identity=28.3653846153846, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI221136756, Length=197, Percent_Identity=28.9340101522843, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI221136753, Length=197, Percent_Identity=28.9340101522843, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI221307494, Length=197, Percent_Identity=28.4263959390863, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI37594471, Length=155, Percent_Identity=30.3225806451613, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI37594469, Length=155, Percent_Identity=30.3225806451613, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI116235487, Length=199, Percent_Identity=27.1356783919598, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI68989263, Length=214, Percent_Identity=27.5700934579439, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=53.8461538461538, Blast_Score=240, Evalue=9e-65,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=40.234375, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1788597, Length=255, Percent_Identity=29.0196078431373, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI221142681, Length=267, Percent_Identity=28.0898876404494, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87082183, Length=256, Percent_Identity=25.78125, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790281, Length=181, Percent_Identity=29.2817679558011, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788682, Length=196, Percent_Identity=26.0204081632653, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=32.7935222672065, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17554946, Length=255, Percent_Identity=34.9019607843137, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17560910, Length=199, Percent_Identity=31.6582914572864, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=187, Percent_Identity=33.6898395721925, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=25.296442687747, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=260, Percent_Identity=26.9230769230769, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=260, Percent_Identity=26.9230769230769, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=229, Percent_Identity=27.0742358078603, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=235, Percent_Identity=28.936170212766, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=180, Percent_Identity=28.8888888888889, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=180, Percent_Identity=28.8888888888889, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=180, Percent_Identity=28.8888888888889, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=160, Percent_Identity=28.75, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=160, Percent_Identity=28.75, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=181, Percent_Identity=28.7292817679558, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=174, Percent_Identity=29.3103448275862, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=35.1020408163265, Blast_Score=138, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=35.1020408163265, Blast_Score=138, Evalue=4e-33,
Organism=Drosophila melanogaster, GI19920382, Length=245, Percent_Identity=31.0204081632653, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24654903, Length=252, Percent_Identity=24.2063492063492, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653139, Length=265, Percent_Identity=29.0566037735849, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21357171, Length=203, Percent_Identity=32.0197044334975, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=25, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24650670, Length=260, Percent_Identity=27.3076923076923, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571730, Length=140, Percent_Identity=31.4285714285714, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571729, Length=140, Percent_Identity=31.4285714285714, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR011968 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27393; Mature: 27261

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAELIVESVGAVRVLKLNRPDRLNALNEAVHEALAAAFAAIEADDSVRAALLTGEGRGF
CCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCE
CAGADLNQSLGGGPRDLGASIDRHYNPLVRRMRALPKPIVAAVNGVAAGAGANLALAADI
EECCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHH
VLACESANFTQAFIRIGLIPDAGGTHFLPRAVGDARARGLAMLGETITARQAADWGLIWR
HHEECCCCHHEEHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
CLPDEGFLAAALAVAADLASRPTQALAAMKAAFNAAGANGLDAQLDLERDLQRRMGRTPD
ECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHCCCCCC
FAEGVAAFREKRPANFTGAPPARD
HHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAELIVESVGAVRVLKLNRPDRLNALNEAVHEALAAAFAAIEADDSVRAALLTGEGRGF
CHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCE
CAGADLNQSLGGGPRDLGASIDRHYNPLVRRMRALPKPIVAAVNGVAAGAGANLALAADI
EECCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHH
VLACESANFTQAFIRIGLIPDAGGTHFLPRAVGDARARGLAMLGETITARQAADWGLIWR
HHEECCCCHHEEHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
CLPDEGFLAAALAVAADLASRPTQALAAMKAAFNAAGANGLDAQLDLERDLQRRMGRTPD
ECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHCCCCCC
FAEGVAAFREKRPANFTGAPPARD
HHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]