Click here to switch to the map view.

The map label for this gene is fixK [H]

Identifier: 148260629

GI number: 148260629

Start: 1801974

End: 1802906

Strand: Reverse

Name: fixK [H]

Synonym: Acry_1631

Alternate gene names: 148260629

Gene position: 1802906-1801974 (Counterclockwise)

Preceding gene: 148260631

Following gene: 148260628

Centisome position: 53.2

GC content: 66.35

Gene sequence:

>933_bases
ATGAACCGCGCCTTGATCGAAATCAACCGGAAGCCCTGTAAGATTCTCGAATTAAAAGACAAAGCGCCGCGCGCGTCGCC
GGTGGCGCAGCTAACCGCACATTCGCGGCCTGAATCATACGAGTTGCGCGACCTTGTTGCTCTGCACTACAAAATGATTG
CTGAGACGTCGGAAGGACGCGAGATGCTGGAGCAGCGGGACCGGGCGCTTCGCTCGCCCATCAATCTTTCGGAAGCCATC
ATTCCAGGCCTGTGCGCGGGCTGCGAGGCGCGGCATATTGGCCTGTGCGATGCGCTGGCGGACGAGGATCTTCATTTCCT
CGCGAGCGTGGCGCGGCAGTCCACGGTGTCGCGCGGCCAGAGCTTCGCGGTGGAGGGCGACAGCGCCCGTTACTTCTTCA
ACATCAATCACGGCACCGCCAAGCTGCACAAGGACCTGCCCGACGGGCGCCGGCAGATCACCGGCTTCGTCGGCGCGGGC
GATTTCGTCGGCCTCGCCGCCGACGACCGCTACACGTTCAGCGCCGAGGCGATCGACGACGTCCGGCTGTGCCGGTTCGA
CCGCGTGGAAATGCGCAACGTGTTCTCCCGCTTTCCCGCGCTCGAGCGCCGGCTGCTCGACGTCGCCTCGCACGAACTCA
TCGTGGCGCAGGAGCAGATGCTGCTGCTCGGCCGCAAGAGCGCGATCGAGCGGCTCGCCAGCTTCATCGACAGCTGGAGC
CGGCGGGCGATGATGTGCGGCGCCCCGCGGGCCGGGCTGCGGCTGCCGATGACCCGCACCGATCTCGCCGATTATCTCGG
CCTGACCATCGAAACCGTCTCCCGCGCGCTCACCGCGCTGCGCAAGCAGGGCGTGATCGACGTGACGCCGGACCACAATA
TCAGCGTCGCCCGCCCGCAGGCCCTGGCCGAAATCGCCCGCGCCGGCGCCTGA

Upstream 100 bases:

>100_bases
CACGGGTCGATGTGCTCGCGGATCATGGGTTCGATCAGGTAAGTCGGTTCATGCATGCAATGTAACATCGTCAGAAAGCA
TGACAGGATCGACCAAGCGG

Downstream 100 bases:

>100_bases
CGCCGGCCCGCTTCCGCGATTGACGCAAGTCAATGCGCGGGCGGAGGGCATCGCGCATGGTCCTCCTAGCCAAAAGGAGG
ATGACATGAACGTGCGTGAG

Product: CRP/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MNRALIEINRKPCKILELKDKAPRASPVAQLTAHSRPESYELRDLVALHYKMIAETSEGREMLEQRDRALRSPINLSEAI
IPGLCAGCEARHIGLCDALADEDLHFLASVARQSTVSRGQSFAVEGDSARYFFNINHGTAKLHKDLPDGRRQITGFVGAG
DFVGLAADDRYTFSAEAIDDVRLCRFDRVEMRNVFSRFPALERRLLDVASHELIVAQEQMLLLGRKSAIERLASFIDSWS
RRAMMCGAPRAGLRLPMTRTDLADYLGLTIETVSRALTALRKQGVIDVTPDHNISVARPQALAEIARAGA

Sequences:

>Translated_310_residues
MNRALIEINRKPCKILELKDKAPRASPVAQLTAHSRPESYELRDLVALHYKMIAETSEGREMLEQRDRALRSPINLSEAI
IPGLCAGCEARHIGLCDALADEDLHFLASVARQSTVSRGQSFAVEGDSARYFFNINHGTAKLHKDLPDGRRQITGFVGAG
DFVGLAADDRYTFSAEAIDDVRLCRFDRVEMRNVFSRFPALERRLLDVASHELIVAQEQMLLLGRKSAIERLASFIDSWS
RRAMMCGAPRAGLRLPMTRTDLADYLGLTIETVSRALTALRKQGVIDVTPDHNISVARPQALAEIARAGA
>Mature_310_residues
MNRALIEINRKPCKILELKDKAPRASPVAQLTAHSRPESYELRDLVALHYKMIAETSEGREMLEQRDRALRSPINLSEAI
IPGLCAGCEARHIGLCDALADEDLHFLASVARQSTVSRGQSFAVEGDSARYFFNINHGTAKLHKDLPDGRRQITGFVGAG
DFVGLAADDRYTFSAEAIDDVRLCRFDRVEMRNVFSRFPALERRLLDVASHELIVAQEQMLLLGRKSAIERLASFIDSWS
RRAMMCGAPRAGLRLPMTRTDLADYLGLTIETVSRALTALRKQGVIDVTPDHNISVARPQALAEIARAGA

Specific function: B.japonicum has two fixLJ-dependent fixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=225, Percent_Identity=32.4444444444444, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 34394; Mature: 34394

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRALIEINRKPCKILELKDKAPRASPVAQLTAHSRPESYELRDLVALHYKMIAETSEGR
CCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHH
EMLEQRDRALRSPINLSEAIIPGLCAGCEARHIGLCDALADEDLHFLASVARQSTVSRGQ
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCC
SFAVEGDSARYFFNINHGTAKLHKDLPDGRRQITGFVGAGDFVGLAADDRYTFSAEAIDD
EEEEECCCEEEEEEECCCHHHHHHCCCCCHHHHEEEECCCCEEEEECCCCEEEEHHHHHH
VRLCRFDRVEMRNVFSRFPALERRLLDVASHELIVAQEQMLLLGRKSAIERLASFIDSWS
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRAMMCGAPRAGLRLPMTRTDLADYLGLTIETVSRALTALRKQGVIDVTPDHNISVARPQ
HCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHH
ALAEIARAGA
HHHHHHHCCC
>Mature Secondary Structure
MNRALIEINRKPCKILELKDKAPRASPVAQLTAHSRPESYELRDLVALHYKMIAETSEGR
CCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHH
EMLEQRDRALRSPINLSEAIIPGLCAGCEARHIGLCDALADEDLHFLASVARQSTVSRGQ
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCC
SFAVEGDSARYFFNINHGTAKLHKDLPDGRRQITGFVGAGDFVGLAADDRYTFSAEAIDD
EEEEECCCEEEEEEECCCHHHHHHCCCCCHHHHEEEECCCCEEEEECCCCEEEEHHHHHH
VRLCRFDRVEMRNVFSRFPALERRLLDVASHELIVAQEQMLLLGRKSAIERLASFIDSWS
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRAMMCGAPRAGLRLPMTRTDLADYLGLTIETVSRALTALRKQGVIDVTPDHNISVARPQ
HCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHH
ALAEIARAGA
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1551834; 12597275 [H]