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The map label for this gene is glcD [H]

Identifier: 148260462

GI number: 148260462

Start: 1624848

End: 1626221

Strand: Reverse

Name: glcD [H]

Synonym: Acry_1462

Alternate gene names: 148260462

Gene position: 1626221-1624848 (Counterclockwise)

Preceding gene: 148260464

Following gene: 148260459

Centisome position: 47.98

GC content: 65.87

Gene sequence:

>1374_bases
ATGGAAACGCAGAGCACGATCGACGCCGCCCGCGCCATTTTCGGCGACCGGCTCAGCCTGAACGCCAATATCCGCGAAAC
CCATGCCCATGGCGAGGCCAATGCAGCCACCAGCCTCCCGGATGCCGTGGTCTTTCCCGAATCGACCGAAGAAGTATCGC
GGTTATTGCGACTTTGCCACGCGACGGACGTACCGGTGACGCCGTTCGGCGCCGGCACTTCGCTCGAGGGCCATGTGACG
CCGGTGCGCGGCGGCATCAGCCTCGACCTCACGCGGATGAACCGCGTACTCGAGGTGAACGACGCCGATCTCGACGGACG
GGTCGAGGCCGGGGTGACCAGGCGGCAGCTGAATACCTATTTGCGCGACCATGGCCTGTTTTTCCCGGTCGACCCTGGGG
CGGATGCAACGATCGGCGGGATGTGCGCGACACGTGCCTCCGGCACGACCACGGTGCGCTACGGCACGATCCGCGACAAT
GTGCTCGGCCTTCGCGCCGTGCTGGCCGATGGCACGGTGATCGACACGGGCGGGCGGGTCCGCAAATCGTCCACCGGCTA
CGACCTCACTCGTTTGCTCATCGGCTCGGAGGGCACGCTCGGCATTATCACCGAGGTGCAACTGAAGCTGTATGGCATTC
CAGAGGCGATCGGCGCTGCGGTGTGCGGCTTTGCTGACGAGCATGCGGCAGTCGCGGCGGTGATCGAGATCATGCAGACT
GGCATCCCTGTCGCCCGCATCGAATTCGCCGACACGGCGCAGATGGCAGCCTCGATTGCCTATTCGAAGCTTGATCACCT
GACTGCCGGCCCGACGCTGATGTTCGAGTTCCACGGCAGCGCGCAAGGCGTTGCCGAGCAGGGAGCGATGGCCGAGGAGA
TCGCGAGGGCGCATGGCGGCAGCGGCTTCGCCTTCGCGACCGAGACCGAGGCGCGCAACCGCCTCTGGCGGGCCCGGCAC
GATGCCTACTGGGCCGCCCGCGCGGCGTATCCCGGCATGGGCGCGTTTGCTACCGACACGATCGTGCCGATCTCGCGGCT
TGCGGAGGCGGTCTCGCTGGCACGCGAGGCGGTCACCCGTTCCGGCCTTGAAGCCTGCATCGTCGGCCATGTCGGCGACG
GCAATTTTCACGTGTTGATCCTCTACCCCGAAGGAGAGGAAGGGCGGGCAAAGGCATGGGAACTCGACCGCGAGATTGTC
GCCCAGGCACTTGCGCTCGGTGGGGCAGCGAGCGGCGAGCATGGGGTCGGGCTCGGCAAGAGGGAGTTTCTGCCCCGCGA
GCATGGGGCCGCAGCACTCGGCGTCATGCGCGCGATCAAGCAAGCCCTTGATCCGCGCGGTATTCTTAATCCGGGAAAGA
TGTTTCTCGACTGA

Upstream 100 bases:

>100_bases
CATGCGGTATTCGCTCCGCGCCGGTTGGGCAAGTCGGCTTGGGCGGCCGGCTTCTGTTCCGGCACATGACTTTGCCGGGA
AATGACGATAGCCTGCCGCC

Downstream 100 bases:

>100_bases
AAGACGGCCTGGCCAAACGGGCCCGGCCGTCTTTCACGCGCCACTCAGTTGGCGTTGGCAGGTGAAGCCGGCGCCGCTGC
GGCTCCAGCGGGCGACGAAC

Product: D-lactate dehydrogenase (cytochrome)

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
METQSTIDAARAIFGDRLSLNANIRETHAHGEANAATSLPDAVVFPESTEEVSRLLRLCHATDVPVTPFGAGTSLEGHVT
PVRGGISLDLTRMNRVLEVNDADLDGRVEAGVTRRQLNTYLRDHGLFFPVDPGADATIGGMCATRASGTTTVRYGTIRDN
VLGLRAVLADGTVIDTGGRVRKSSTGYDLTRLLIGSEGTLGIITEVQLKLYGIPEAIGAAVCGFADEHAAVAAVIEIMQT
GIPVARIEFADTAQMAASIAYSKLDHLTAGPTLMFEFHGSAQGVAEQGAMAEEIARAHGGSGFAFATETEARNRLWRARH
DAYWAARAAYPGMGAFATDTIVPISRLAEAVSLAREAVTRSGLEACIVGHVGDGNFHVLILYPEGEEGRAKAWELDREIV
AQALALGGAASGEHGVGLGKREFLPREHGAAALGVMRAIKQALDPRGILNPGKMFLD

Sequences:

>Translated_457_residues
METQSTIDAARAIFGDRLSLNANIRETHAHGEANAATSLPDAVVFPESTEEVSRLLRLCHATDVPVTPFGAGTSLEGHVT
PVRGGISLDLTRMNRVLEVNDADLDGRVEAGVTRRQLNTYLRDHGLFFPVDPGADATIGGMCATRASGTTTVRYGTIRDN
VLGLRAVLADGTVIDTGGRVRKSSTGYDLTRLLIGSEGTLGIITEVQLKLYGIPEAIGAAVCGFADEHAAVAAVIEIMQT
GIPVARIEFADTAQMAASIAYSKLDHLTAGPTLMFEFHGSAQGVAEQGAMAEEIARAHGGSGFAFATETEARNRLWRARH
DAYWAARAAYPGMGAFATDTIVPISRLAEAVSLAREAVTRSGLEACIVGHVGDGNFHVLILYPEGEEGRAKAWELDREIV
AQALALGGAASGEHGVGLGKREFLPREHGAAALGVMRAIKQALDPRGILNPGKMFLD
>Mature_457_residues
METQSTIDAARAIFGDRLSLNANIRETHAHGEANAATSLPDAVVFPESTEEVSRLLRLCHATDVPVTPFGAGTSLEGHVT
PVRGGISLDLTRMNRVLEVNDADLDGRVEAGVTRRQLNTYLRDHGLFFPVDPGADATIGGMCATRASGTTTVRYGTIRDN
VLGLRAVLADGTVIDTGGRVRKSSTGYDLTRLLIGSEGTLGIITEVQLKLYGIPEAIGAAVCGFADEHAAVAAVIEIMQT
GIPVARIEFADTAQMAASIAYSKLDHLTAGPTLMFEFHGSAQGVAEQGAMAEEIARAHGGSGFAFATETEARNRLWRARH
DAYWAARAAYPGMGAFATDTIVPISRLAEAVSLAREAVTRSGLEACIVGHVGDGNFHVLILYPEGEEGRAKAWELDREIV
AQALALGGAASGEHGVGLGKREFLPREHGAAALGVMRAIKQALDPRGILNPGKMFLD

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=456, Percent_Identity=50.8771929824561, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI37595754, Length=479, Percent_Identity=48.4342379958246, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI119964728, Length=423, Percent_Identity=34.2789598108747, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI4501993, Length=452, Percent_Identity=26.9911504424779, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1789351, Length=424, Percent_Identity=30.8962264150943, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1787977, Length=497, Percent_Identity=30.1810865191147, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI48994907, Length=450, Percent_Identity=26.2222222222222, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI48994914, Length=172, Percent_Identity=29.0697674418605, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71992373, Length=459, Percent_Identity=42.9193899782135, Blast_Score=370, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71992381, Length=459, Percent_Identity=41.6122004357298, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI71992392, Length=379, Percent_Identity=45.646437994723, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI17534361, Length=431, Percent_Identity=27.1461716937355, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17556096, Length=455, Percent_Identity=26.1538461538462, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6320027, Length=429, Percent_Identity=40.5594405594406, Blast_Score=319, Evalue=5e-88,
Organism=Saccharomyces cerevisiae, GI6320764, Length=436, Percent_Identity=29.5871559633028, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6320023, Length=446, Percent_Identity=29.372197309417, Blast_Score=162, Evalue=7e-41,
Organism=Drosophila melanogaster, GI18921117, Length=423, Percent_Identity=30.2600472813239, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24639277, Length=423, Percent_Identity=30.2600472813239, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24639275, Length=423, Percent_Identity=30.2600472813239, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24653753, Length=458, Percent_Identity=27.5109170305677, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.1.28

Molecular weight: Translated: 48272; Mature: 48272

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METQSTIDAARAIFGDRLSLNANIRETHAHGEANAATSLPDAVVFPESTEEVSRLLRLCH
CCCHHHHHHHHHHHCCCEEECCCCHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHH
ATDVPVTPFGAGTSLEGHVTPVRGGISLDLTRMNRVLEVNDADLDGRVEAGVTRRQLNTY
CCCCCCCCCCCCCCCCCCEEEECCCCEEEHHHCCCEEEECCCCCCCCHHCCHHHHHHHHH
LRDHGLFFPVDPGADATIGGMCATRASGTTTVRYGTIRDNVLGLRAVLADGTVIDTGGRV
HHHCCCEEECCCCCCCCCCCEEECCCCCCEEEEECCCCCCHHHHHHHHCCCCEEECCCCE
RKSSTGYDLTRLLIGSEGTLGIITEVQLKLYGIPEAIGAAVCGFADEHAAVAAVIEIMQT
ECCCCCCCEEEEEECCCCCEEEEEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHC
GIPVARIEFADTAQMAASIAYSKLDHLTAGPTLMFEFHGSAQGVAEQGAMAEEIARAHGG
CCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCHHHHHHHHHCCC
SGFAFATETEARNRLWRARHDAYWAARAAYPGMGAFATDTIVPISRLAEAVSLAREAVTR
CCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SGLEACIVGHVGDGNFHVLILYPEGEEGRAKAWELDREIVAQALALGGAASGEHGVGLGK
CCCCEEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
REFLPREHGAAALGVMRAIKQALDPRGILNPGKMFLD
HHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCC
>Mature Secondary Structure
METQSTIDAARAIFGDRLSLNANIRETHAHGEANAATSLPDAVVFPESTEEVSRLLRLCH
CCCHHHHHHHHHHHCCCEEECCCCHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHH
ATDVPVTPFGAGTSLEGHVTPVRGGISLDLTRMNRVLEVNDADLDGRVEAGVTRRQLNTY
CCCCCCCCCCCCCCCCCCEEEECCCCEEEHHHCCCEEEECCCCCCCCHHCCHHHHHHHHH
LRDHGLFFPVDPGADATIGGMCATRASGTTTVRYGTIRDNVLGLRAVLADGTVIDTGGRV
HHHCCCEEECCCCCCCCCCCEEECCCCCCEEEEECCCCCCHHHHHHHHCCCCEEECCCCE
RKSSTGYDLTRLLIGSEGTLGIITEVQLKLYGIPEAIGAAVCGFADEHAAVAAVIEIMQT
ECCCCCCCEEEEEECCCCCEEEEEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHC
GIPVARIEFADTAQMAASIAYSKLDHLTAGPTLMFEFHGSAQGVAEQGAMAEEIARAHGG
CCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCHHHHHHHHHCCC
SGFAFATETEARNRLWRARHDAYWAARAAYPGMGAFATDTIVPISRLAEAVSLAREAVTR
CCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SGLEACIVGHVGDGNFHVLILYPEGEEGRAKAWELDREIVAQALALGGAASGEHGVGLGK
CCCCEEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
REFLPREHGAAALGVMRAIKQALDPRGILNPGKMFLD
HHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]