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The map label for this gene is smeIM [H]

Identifier: 148260450

GI number: 148260450

Start: 1609664

End: 1610746

Strand: Reverse

Name: smeIM [H]

Synonym: Acry_1449

Alternate gene names: 148260450

Gene position: 1610746-1609664 (Counterclockwise)

Preceding gene: 148260452

Following gene: 148260449

Centisome position: 47.53

GC content: 64.91

Gene sequence:

>1083_bases
TTGCCCTTGACCGCCGTTCGCGAATTGCCGCTCGATCAGCTTCTGCTTGGCGATGCCGTCGAGATGATGCGCATGCTGCC
GGATGCGTCGATCGACTGCGTCTTCGCCGATCCGCCCTACAACCTGCAACTCCGTGGCGAATTGCGCCGGCCGGATGACA
GTGTGGTCGACGGTGTTGACGACGACTGGGACAAGTTCACCGATTTCGCGGCCTATGACGAATTCTCGCGCGCCTGGCTC
TCTGAGGCGCGGCGGCTGATGCACAAGGACGCGACGATCTGGGTGATCGGCTCCTATCACAACATTTTCCGCCTCGGCGC
GATCATGCAGGATCTCGGGTTCTGGATTCTCAACGACGTGATCTGGCGCAAGACCAACCCGATGCCGAATTTCCGCGGCC
GGCGCTTCACGAATGCGCACGAAACGCTGATCTGGGCGGCGCGGGAGCCGAAATCGCGTTACCGGTTCAACTATCAGGCG
ATGAAATCGCTCAACGACGATACCCAGATGCGCAGCGACTGGACGATTCCGCTCTGCACCGGCGGCGAGCGGCTGCGCAA
CCAGCACGGCCTCAAGCTGCACCCGACCCAGAAGCCGGAGGCCCTGCTGCACCGCGTGATCCTGGCCGCGACCAACCCGG
ACGACGTGGTGCTGGACCCGTTTTCTGGTACCGCGACGACGGCGACGGTGGCCAAGCGGCTCAACCGGCATTTCATCGGC
ATCGAGCGTCATCCCGCCTACGTCGAGGCCGGCTGGGCGCGGCTGCGGGAAATGCGGGCGGCGCCGGCGCATCTGACCAG
GCCGATGCCGAGCCGCAGAGAGGCGCCGCGTATCGCCTTCGGCGTGCTGGTCGAGCGCGGCTTGGTCCCGCCAGGCACAT
TGCTGCATGACAGGCTGCGGCGGGTGAGCGCCACGGTCGCGGCGGATGCCAGCATCATCGCCGGCGCGCATCGCGGCTCG
ATCCATCAGGTCGGTGCCGCGGTTCAGAACGCCGCAAGCTGCAATGGCTGGACGTTCTGGCATATCGAGCGCGAGGGGAG
GCTTGTCGCACTTGATCATGTGCGCGAAACGTTGCGTCCATGA

Upstream 100 bases:

>100_bases
CGGATGCAAGCCGCAAGGTCGGTCCGGGTGCGCATCAGCCATTGACGCGACCACGGCGCGTTCGTCAGGTATAGGGGGGA
GAGACAGACCGAGGCCCAAA

Downstream 100 bases:

>100_bases
CCTCCACCCTTGCCTCGATGACCGGCTTTGCCCGCCACGCGGGCAGCCACGACAATGCCAGTTTCGTCTGGGAAATCCGC
AGCGTGAACGGTCGCGGGCT

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.SmeI; Adenine-specific methyltransferase SmeIP; M.CcrMI ortholog; Modification methylase SmeIP; M.SmeIP [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MPLTAVRELPLDQLLLGDAVEMMRMLPDASIDCVFADPPYNLQLRGELRRPDDSVVDGVDDDWDKFTDFAAYDEFSRAWL
SEARRLMHKDATIWVIGSYHNIFRLGAIMQDLGFWILNDVIWRKTNPMPNFRGRRFTNAHETLIWAAREPKSRYRFNYQA
MKSLNDDTQMRSDWTIPLCTGGERLRNQHGLKLHPTQKPEALLHRVILAATNPDDVVLDPFSGTATTATVAKRLNRHFIG
IERHPAYVEAGWARLREMRAAPAHLTRPMPSRREAPRIAFGVLVERGLVPPGTLLHDRLRRVSATVAADASIIAGAHRGS
IHQVGAAVQNAASCNGWTFWHIEREGRLVALDHVRETLRP

Sequences:

>Translated_360_residues
MPLTAVRELPLDQLLLGDAVEMMRMLPDASIDCVFADPPYNLQLRGELRRPDDSVVDGVDDDWDKFTDFAAYDEFSRAWL
SEARRLMHKDATIWVIGSYHNIFRLGAIMQDLGFWILNDVIWRKTNPMPNFRGRRFTNAHETLIWAAREPKSRYRFNYQA
MKSLNDDTQMRSDWTIPLCTGGERLRNQHGLKLHPTQKPEALLHRVILAATNPDDVVLDPFSGTATTATVAKRLNRHFIG
IERHPAYVEAGWARLREMRAAPAHLTRPMPSRREAPRIAFGVLVERGLVPPGTLLHDRLRRVSATVAADASIIAGAHRGS
IHQVGAAVQNAASCNGWTFWHIEREGRLVALDHVRETLRP
>Mature_359_residues
PLTAVRELPLDQLLLGDAVEMMRMLPDASIDCVFADPPYNLQLRGELRRPDDSVVDGVDDDWDKFTDFAAYDEFSRAWLS
EARRLMHKDATIWVIGSYHNIFRLGAIMQDLGFWILNDVIWRKTNPMPNFRGRRFTNAHETLIWAAREPKSRYRFNYQAM
KSLNDDTQMRSDWTIPLCTGGERLRNQHGLKLHPTQKPEALLHRVILAATNPDDVVLDPFSGTATTATVAKRLNRHFIGI
ERHPAYVEAGWARLREMRAAPAHLTRPMPSRREAPRIAFGVLVERGLVPPGTLLHDRLRRVSATVAADASIIAGAHRGSI
HQVGAAVQNAASCNGWTFWHIEREGRLVALDHVRETLRP

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=269, Percent_Identity=28.996282527881, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 40912; Mature: 40781

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTAVRELPLDQLLLGDAVEMMRMLPDASIDCVFADPPYNLQLRGELRRPDDSVVDGVD
CCCCHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCCHHHHCCCC
DDWDKFTDFAAYDEFSRAWLSEARRLMHKDATIWVIGSYHNIFRLGAIMQDLGFWILNDV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
IWRKTNPMPNFRGRRFTNAHETLIWAAREPKSRYRFNYQAMKSLNDDTQMRSDWTIPLCT
HHHCCCCCCCCCCCEECCCCCEEEEECCCCHHHHEECHHHHHCCCCCHHHCCCCCEEEEC
GGERLRNQHGLKLHPTQKPEALLHRVILAATNPDDVVLDPFSGTATTATVAKRLNRHFIG
CHHHHHHCCCCEECCCCCHHHHHHHHHHEECCCCCEEEECCCCCHHHHHHHHHHHHHEEC
IERHPAYVEAGWARLREMRAAPAHLTRPMPSRREAPRIAFGVLVERGLVPPGTLLHDRLR
CCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
RVSATVAADASIIAGAHRGSIHQVGAAVQNAASCNGWTFWHIEREGRLVALDHVRETLRP
HHHHHHHCCHHHEECCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCEEEHHHHHHHHCC
>Mature Secondary Structure 
PLTAVRELPLDQLLLGDAVEMMRMLPDASIDCVFADPPYNLQLRGELRRPDDSVVDGVD
CCCHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCCHHHHCCCC
DDWDKFTDFAAYDEFSRAWLSEARRLMHKDATIWVIGSYHNIFRLGAIMQDLGFWILNDV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
IWRKTNPMPNFRGRRFTNAHETLIWAAREPKSRYRFNYQAMKSLNDDTQMRSDWTIPLCT
HHHCCCCCCCCCCCEECCCCCEEEEECCCCHHHHEECHHHHHCCCCCHHHCCCCCEEEEC
GGERLRNQHGLKLHPTQKPEALLHRVILAATNPDDVVLDPFSGTATTATVAKRLNRHFIG
CHHHHHHCCCCEECCCCCHHHHHHHHHHEECCCCCEEEECCCCCHHHHHHHHHHHHHEEC
IERHPAYVEAGWARLREMRAAPAHLTRPMPSRREAPRIAFGVLVERGLVPPGTLLHDRLR
CCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
RVSATVAADASIIAGAHRGSIHQVGAAVQNAASCNGWTFWHIEREGRLVALDHVRETLRP
HHHHHHHCCHHHEECCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9294447; 11481430 [H]