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The map label for this gene is dnaN [H]

Identifier: 148260438

GI number: 148260438

Start: 1597010

End: 1598134

Strand: Reverse

Name: dnaN [H]

Synonym: Acry_1437

Alternate gene names: 148260438

Gene position: 1598134-1597010 (Counterclockwise)

Preceding gene: 148260442

Following gene: 148260436

Centisome position: 47.15

GC content: 66.04

Gene sequence:

>1125_bases
ATGAAGCTCAAGGCGGATCGGGCAACGCTGCTGAAGGCGCTGGCCCATGTTCAGAACGTTGTCGAAAAACGCAACACGAT
TCCCATCCTGGCCAATGTGCTGTTGTCGGTGAAGGAAGGCCGGCTGACCATCGCCGCGACCGATCTGGAGATCGCGCTGA
TCGAGGAGGTCGCCGCGACGACGATGAGGGACGGCTCTGTCACCGCGCCGGCGGCGACCCTTTACGAGATCGTGCGCCGC
CAGCCGGACAACGCCGAGATCGAGCTCGACCATCCGGGCGGTGACGCGCCGCTCGCGCTGCGGGCAGGACGCTACGCCAC
CAAGCTCGTGGTGCTGCCGACCGACGAATTCCCCAAGCTCGATGCCGGCAAGCTGACGCATCGCTTCGCCGTGCCGGCGC
AGGCGCTGCGCGGGCTGATCGACCGCACGCGCTTCGCGATCTCGACCGAGGAAACCCGCTACTATCTCAACGGCATCTAT
CTTCATGCCGCCGAGTCGGAGGGAGTGCCGGTGCTTCGCGCCGTGGCGACGGACGGGCATCGTCTCGCCCGCGTCGAGGA
GCCGCTGCCCGAGGGCGCCGCGGGGATGCCCGGCGTCATCGTGCCGCGCAAGACGGTGAACGAGATCCGCAAGCTGCTCG
ACGAGGTCTCCGGCGATGTCTCGATCGCGCTCTCGGAAACCCGCATCCAGTTCGAGCTGGACACCATGCGGATGACCAGC
AAGCTGATCGACGGCACCTATCCCGATTATTCGAAGGTGATCCCTACGGGCAACGACAAGATACTGCGCGTCGAGACCGC
CGATTTCTCCGCCGCAGTGGCGCGCGTCTCGGCGATCACCTCCGAGCGAAACAAGCCGGTCAAACTCTCCGTCGGCCGCG
ACCTCCTGGTGCTTTCGGCGACCAGCCCCGACCAGGGCACGGCAACCGAGGAACTCGACGCCGAGCAGGTGGAATACCGT
GCCACCCCGCTCGAAATCGGCTTCCAGGCCCGCTACCTCAACGACGTGACGGAACAGATCAAGGGCAAGGTGGAGTTCTG
CTTCGCCGACGGCGCGGCGCCGACCCTGGTGCGCGCCGCCGGCGACGAGAGCGCGATTTTCGTTCTCATGCCGATGCGGG
TCTGA

Upstream 100 bases:

>100_bases
CACCATCGGGCGGCGGGATGGTTCAATTCCGGTTCGAACTGGGCTATGCAGCGAGACCCGGGTGCTGAGGCAGCCCGGTC
AGGCTGACTGGAAGGATCGC

Downstream 100 bases:

>100_bases
CCCCGCGGTGCCGCGCCGGGGCCTCCGGGCGCGGCGCCGGAGGCTTCACCCCTGCATGGAGCCGTTCTCGCGCAGGCGGG
CGTGCCAGGACAGCGCCTGT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKLKADRATLLKALAHVQNVVEKRNTIPILANVLLSVKEGRLTIAATDLEIALIEEVAATTMRDGSVTAPAATLYEIVRR
QPDNAEIELDHPGGDAPLALRAGRYATKLVVLPTDEFPKLDAGKLTHRFAVPAQALRGLIDRTRFAISTEETRYYLNGIY
LHAAESEGVPVLRAVATDGHRLARVEEPLPEGAAGMPGVIVPRKTVNEIRKLLDEVSGDVSIALSETRIQFELDTMRMTS
KLIDGTYPDYSKVIPTGNDKILRVETADFSAAVARVSAITSERNKPVKLSVGRDLLVLSATSPDQGTATEELDAEQVEYR
ATPLEIGFQARYLNDVTEQIKGKVEFCFADGAAPTLVRAAGDESAIFVLMPMRV

Sequences:

>Translated_374_residues
MKLKADRATLLKALAHVQNVVEKRNTIPILANVLLSVKEGRLTIAATDLEIALIEEVAATTMRDGSVTAPAATLYEIVRR
QPDNAEIELDHPGGDAPLALRAGRYATKLVVLPTDEFPKLDAGKLTHRFAVPAQALRGLIDRTRFAISTEETRYYLNGIY
LHAAESEGVPVLRAVATDGHRLARVEEPLPEGAAGMPGVIVPRKTVNEIRKLLDEVSGDVSIALSETRIQFELDTMRMTS
KLIDGTYPDYSKVIPTGNDKILRVETADFSAAVARVSAITSERNKPVKLSVGRDLLVLSATSPDQGTATEELDAEQVEYR
ATPLEIGFQARYLNDVTEQIKGKVEFCFADGAAPTLVRAAGDESAIFVLMPMRV
>Mature_374_residues
MKLKADRATLLKALAHVQNVVEKRNTIPILANVLLSVKEGRLTIAATDLEIALIEEVAATTMRDGSVTAPAATLYEIVRR
QPDNAEIELDHPGGDAPLALRAGRYATKLVVLPTDEFPKLDAGKLTHRFAVPAQALRGLIDRTRFAISTEETRYYLNGIY
LHAAESEGVPVLRAVATDGHRLARVEEPLPEGAAGMPGVIVPRKTVNEIRKLLDEVSGDVSIALSETRIQFELDTMRMTS
KLIDGTYPDYSKVIPTGNDKILRVETADFSAAVARVSAITSERNKPVKLSVGRDLLVLSATSPDQGTATEELDAEQVEYR
ATPLEIGFQARYLNDVTEQIKGKVEFCFADGAAPTLVRAAGDESAIFVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=376, Percent_Identity=35.3723404255319, Blast_Score=215, Evalue=4e-57,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40681; Mature: 40681

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKADRATLLKALAHVQNVVEKRNTIPILANVLLSVKEGRLTIAATDLEIALIEEVAAT
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHH
TMRDGSVTAPAATLYEIVRRQPDNAEIELDHPGGDAPLALRAGRYATKLVVLPTDEFPKL
HCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCEEEEEEEEECCCCCCC
DAGKLTHRFAVPAQALRGLIDRTRFAISTEETRYYLNGIYLHAAESEGVPVLRAVATDGH
CCCCCHHHCCCHHHHHHHHHHHHHEEEECCCCEEEEEEEEEEECCCCCCCEEEEEECCCC
RLARVEEPLPEGAAGMPGVIVPRKTVNEIRKLLDEVSGDVSIALSETRIQFELDTMRMTS
CEEHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEHHHHHHH
KLIDGTYPDYSKVIPTGNDKILRVETADFSAAVARVSAITSERNKPVKLSVGRDLLVLSA
HHHCCCCCCHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEE
TSPDQGTATEELDAEQVEYRATPLEIGFQARYLNDVTEQIKGKVEFCFADGAAPTLVRAA
CCCCCCCCCHHCCHHHHHEECCCEEECCHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEC
GDESAIFVLMPMRV
CCCCEEEEEEECCC
>Mature Secondary Structure
MKLKADRATLLKALAHVQNVVEKRNTIPILANVLLSVKEGRLTIAATDLEIALIEEVAAT
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHH
TMRDGSVTAPAATLYEIVRRQPDNAEIELDHPGGDAPLALRAGRYATKLVVLPTDEFPKL
HCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCEEEEEEEEECCCCCCC
DAGKLTHRFAVPAQALRGLIDRTRFAISTEETRYYLNGIYLHAAESEGVPVLRAVATDGH
CCCCCHHHCCCHHHHHHHHHHHHHEEEECCCCEEEEEEEEEEECCCCCCCEEEEEECCCC
RLARVEEPLPEGAAGMPGVIVPRKTVNEIRKLLDEVSGDVSIALSETRIQFELDTMRMTS
CEEHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEHHHHHHH
KLIDGTYPDYSKVIPTGNDKILRVETADFSAAVARVSAITSERNKPVKLSVGRDLLVLSA
HHHCCCCCCHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEE
TSPDQGTATEELDAEQVEYRATPLEIGFQARYLNDVTEQIKGKVEFCFADGAAPTLVRAA
CCCCCCCCCHHCCHHHHHEECCCEEECCHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEC
GDESAIFVLMPMRV
CCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]