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The map label for this gene is ispB [H]

Identifier: 148260434

GI number: 148260434

Start: 1592719

End: 1593753

Strand: Reverse

Name: ispB [H]

Synonym: Acry_1432

Alternate gene names: 148260434

Gene position: 1593753-1592719 (Counterclockwise)

Preceding gene: 148260436

Following gene: 148260433

Centisome position: 47.02

GC content: 65.99

Gene sequence:

>1035_bases
GTGGATGGGCTGCTGACCTCGGGACAGGATGCCGGAACGGCGCGCACGCGTCCGGAGGAAGATGCCTTGCATCGCCTCGC
GGTGGTCCTTCAGGACGATCTGACGGAAACGAACCGCACCATCCTCGCGCGCATGGCGAGCGATGTGGAGCTGATCCCGC
AACTTGCTGCGCATCTGATCGCCGCGGGTGGCAAGCGGTTGCGGCCGCTTCTCACCCTCGCCGCGGCGCGTCTCTGCGGC
TATCGCGGCGAGCGGCATATCCACCTCGCCGCCTGTGTCGAATTCATCCACACAGCCACGCTGCTGCATGACGATGTGGT
GGATGAAAGCGGCCTCCGCCGCGGGCTCGCGAGCGCGAACGCCGTGTTTGGCAACAAGGCTTCGGTGCTGGTGGGGGATT
TCCTGTTTGCCCGTGCCTTCGAGCTGATGGTCGAGGACGGCTCGCTGGACGTGCTGCGAATTCTCTGCAACGCCTCGGCT
ACCATCGCCGAGGGCGAGGTGCTCCAGCTTACGACCCAGAATGATCTCAGCACGGACATTCCGCGCTATTTCAAGGTGAT
CGAGGGCAAGACCGCGGCGCTGTTCGCGGCGGCGTGCGAGGTGGGCGGTGTTGTTGCCGACCGTGACTCTGCGGTGTGCG
GCGCGCTTGCCTGCTACGGCGCGGCGCTCGGCATGGCGTTCCAGCTCGTCGATGACGCACTCGATTATGCGGCCGACCAG
AACGAACTGGGCAAGACCGTCGGCGACGATTTCCGCGAGGGCAAGCTGACCTATCCGGTCCTGGTGGCCTATGCGGCGGG
CGATGGTCCTGAGCGCGCGTTCTGGCGCCGTACCATCGAGGACGGCGAGCAGACCGAAACCGATCTCGCCACAGCTCTCG
ATCTGATCCGCAGGCATGACGCCATCGGCCAGACCATCCGCGAGGCCGACGCATTCGCGCGCAAGGCGAAGGACGCACTG
GCCACGCTGCCGGACGATCCGCTGCGCGCCCTGATGATGGAGGCGGCGGATTATGCCGTCTCCCGCCATCGCTGA

Upstream 100 bases:

>100_bases
TTTCCATGCGGTCTCCTTCCCCGCGGGCCGCCTTGACCCTCTGTCCCCCGCGCCGATATGCTGTCCTGATCCAGATGGAA
ACACAAGGTTGATACCGGCT

Downstream 100 bases:

>100_bases
ACCCGCTACCCGGAGAGACGCCACATGACCACGCCGCATCTGCGCGGACTCTGTGCCGAATGGGGCCGGATGAGCCGGGC
GGAGCGTGACCGCGCCTATG

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MDGLLTSGQDAGTARTRPEEDALHRLAVVLQDDLTETNRTILARMASDVELIPQLAAHLIAAGGKRLRPLLTLAAARLCG
YRGERHIHLAACVEFIHTATLLHDDVVDESGLRRGLASANAVFGNKASVLVGDFLFARAFELMVEDGSLDVLRILCNASA
TIAEGEVLQLTTQNDLSTDIPRYFKVIEGKTAALFAAACEVGGVVADRDSAVCGALACYGAALGMAFQLVDDALDYAADQ
NELGKTVGDDFREGKLTYPVLVAYAAGDGPERAFWRRTIEDGEQTETDLATALDLIRRHDAIGQTIREADAFARKAKDAL
ATLPDDPLRALMMEAADYAVSRHR

Sequences:

>Translated_344_residues
MDGLLTSGQDAGTARTRPEEDALHRLAVVLQDDLTETNRTILARMASDVELIPQLAAHLIAAGGKRLRPLLTLAAARLCG
YRGERHIHLAACVEFIHTATLLHDDVVDESGLRRGLASANAVFGNKASVLVGDFLFARAFELMVEDGSLDVLRILCNASA
TIAEGEVLQLTTQNDLSTDIPRYFKVIEGKTAALFAAACEVGGVVADRDSAVCGALACYGAALGMAFQLVDDALDYAADQ
NELGKTVGDDFREGKLTYPVLVAYAAGDGPERAFWRRTIEDGEQTETDLATALDLIRRHDAIGQTIREADAFARKAKDAL
ATLPDDPLRALMMEAADYAVSRHR
>Mature_344_residues
MDGLLTSGQDAGTARTRPEEDALHRLAVVLQDDLTETNRTILARMASDVELIPQLAAHLIAAGGKRLRPLLTLAAARLCG
YRGERHIHLAACVEFIHTATLLHDDVVDESGLRRGLASANAVFGNKASVLVGDFLFARAFELMVEDGSLDVLRILCNASA
TIAEGEVLQLTTQNDLSTDIPRYFKVIEGKTAALFAAACEVGGVVADRDSAVCGALACYGAALGMAFQLVDDALDYAADQ
NELGKTVGDDFREGKLTYPVLVAYAAGDGPERAFWRRTIEDGEQTETDLATALDLIRRHDAIGQTIREADAFARKAKDAL
ATLPDDPLRALMMEAADYAVSRHR

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=300, Percent_Identity=29, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1789578, Length=323, Percent_Identity=47.3684210526316, Blast_Score=264, Evalue=8e-72,
Organism=Escherichia coli, GI1786623, Length=241, Percent_Identity=33.6099585062241, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=304, Percent_Identity=34.2105263157895, Blast_Score=164, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6319475, Length=301, Percent_Identity=30.5647840531561, Blast_Score=122, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6325188, Length=221, Percent_Identity=24.4343891402715, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24651612, Length=328, Percent_Identity=29.8780487804878, Blast_Score=134, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36981; Mature: 36981

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGLLTSGQDAGTARTRPEEDALHRLAVVLQDDLTETNRTILARMASDVELIPQLAAHLI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGGKRLRPLLTLAAARLCGYRGERHIHLAACVEFIHTATLLHDDVVDESGLRRGLASAN
HHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
AVFGNKASVLVGDFLFARAFELMVEDGSLDVLRILCNASATIAEGEVLQLTTQNDLSTDI
HHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEECCCCEEEEECCCCCCHHH
PRYFKVIEGKTAALFAAACEVGGVVADRDSAVCGALACYGAALGMAFQLVDDALDYAADQ
HHHHHHHCCCHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
NELGKTVGDDFREGKLTYPVLVAYAAGDGPERAFWRRTIEDGEQTETDLATALDLIRRHD
HHHHHHHCCHHHCCCCCHHEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AIGQTIREADAFARKAKDALATLPDDPLRALMMEAADYAVSRHR
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDGLLTSGQDAGTARTRPEEDALHRLAVVLQDDLTETNRTILARMASDVELIPQLAAHLI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGGKRLRPLLTLAAARLCGYRGERHIHLAACVEFIHTATLLHDDVVDESGLRRGLASAN
HHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
AVFGNKASVLVGDFLFARAFELMVEDGSLDVLRILCNASATIAEGEVLQLTTQNDLSTDI
HHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEECCCCEEEEECCCCCCHHH
PRYFKVIEGKTAALFAAACEVGGVVADRDSAVCGALACYGAALGMAFQLVDDALDYAADQ
HHHHHHHCCCHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
NELGKTVGDDFREGKLTYPVLVAYAAGDGPERAFWRRTIEDGEQTETDLATALDLIRRHD
HHHHHHHCCHHHCCCCCHHEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AIGQTIREADAFARKAKDALATLPDDPLRALMMEAADYAVSRHR
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]