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The map label for this gene is thiC [H]

Identifier: 148259975

GI number: 148259975

Start: 1066951

End: 1068771

Strand: Reverse

Name: thiC [H]

Synonym: Acry_0965

Alternate gene names: 148259975

Gene position: 1068771-1066951 (Counterclockwise)

Preceding gene: 148259983

Following gene: 148259974

Centisome position: 31.53

GC content: 67.16

Gene sequence:

>1821_bases
ATGAACGTCCAGACCAAGCCTGCCAGTGTCACGACCGGCCCGATCGCCGGCTCGCGCAAGGTGTTTACCTCGCCCGAGGG
GCGGCCCGACATCGCCGTGCCGTTCCGCGAGGTCGACCTGCACCCCAGCGCGGGGGAAGCGCCGTTCCGGCTCTATGACA
CGTCGGGGCCGTTCACCGACCCGCATTTCAGTCTCGATCTCGACAACGGGCTGCCGCTGGTGCGCGGCGCCTGGATCGAG
CGGCGCGGATTCGATGCCGTGGCCGGCCGGGCGGTGAAGCCGGAGGATAACGGATTCGCGGCGGCGGACCGGCTGGTGCC
CCCCTGCCCCGCCGGGCGGAGCGTGTTCGCCGGCAAGGATGGTGCGCTGGTGACGCAGTATGAATACGCGCGGGCCGGGA
TCGTCACGGAAGAGATGATCTATGTCGCGCATCGCGAAAATCTCGGCCGGGCGAAGGCCGCCGAAGGCGCGGCGGCGCGG
CGGGCGGATGGCGAGCGGTTCGGCGCGGCGATCCCCGAATTCGTGACGCCGGAATTCGTCCGCAGCGAAATCGCGGCGGG
ACGGGCGATCATTCCGGCGAACATCAACCACCCCGAGCTGGAGCCGGTGATCATCGGGCGCAATTTCCTCGTCAAGATCA
ACGCCAATATCGGCAACTCGGCGGTCACCTCGGGGGCCGCGGAAGAGGTCGAGAAGCTGGTCTGGGCGATTCGCTGGGGC
GCGGATACGGTGATGGACCTCTCCACCGGGCGGAACATCCACAACATCCGCTCCTGGATCATGCGCAACTCGCCGGTGCC
GATCGGCACCGTGCCGATCTACCAGGCGCTTGAGAAGGTCGGCGGTGATCCGGACAAGCTCGACTGGGAGGTGTTCCGCG
ACACGCTGATCGAGCAGGCCGAGCAGGGCGTGGACTATTTCACCATCCATGCCGGGGTGCGGCTCGCCCATGTGCCGCTG
ACGGCGAAGCGGGTGACCGGCATCGTCTCGCGCGGCGGGTCGATCATGGCGCGCTGGTGCCTCGCGCATCACCGCGAGAG
CTTCCTCTACGAGAAGTTCGACGAGATCTGCGACATCATGCGCAAGTATGACGTGTCGTTCTCGCTGGGCGACGGGCTGC
GGCCCGGCTCGATCGCCGATGCCAATGACGCGGCGCAGTTCGCCGAGCTGGAGACGCTGGGCGAGCTGACGAAGATCGCC
TGGGCCAAGGGCTGCCAGGTGATGATCGAGGGCCCCGGCCATGTGCCGATGCACAAGATCAAGGAGAACATGGAAAAGCA
GCTCGAGGTCTGCCACGAGGCGCCGTTCTATACGCTCGGGCCGCTGACCACCGACATCGCGCCGGGCTACGATCACATCA
CCTCGGCGATCGGGGCGGCGATGATCGGCTGGTTCGGCACCGCGATGCTCTGCTACGTCACGCCGAAGGAGCATCTCGGC
CTGCCCAACCGCGACGATGTGAAGACCGGCGTCATCACCTACCGTCTTGCGGCGCACGCGGCGGATCTCGCCAAGGGGCA
TCCGGCGGCGAAGCTGCGCGACGACGCGGTGAGCCGGGCGCGCTTCGAGTTCCGCTGGGAGGACCAGTTCAATCTCGGCC
TCGACCCGGACACGGCGCGCGCCTTCCACGACGAGACGATGCCCAAGGAGGCGCACAAGGTCGCGCATTTCTGCTCGATG
TGCGGGCCGAAATTCTGCTCGATGAAGATCACCCAGGACATTCGCGACGAGGTGGCGCGCGAGGAAGGGCTCGCGCAGAA
GGCGGCGGAGTTCCGCGATGGCGGCAGCCGCATCTATGTCGAGGATCAGGCGGCGGAGTGA

Upstream 100 bases:

>100_bases
GCAACCCTTTGAACCTGATCCGGGTTGCGCCGGCGGAGGGACGGGATCATCCAGACCGAACCATACGCCCCAGCGTCGCC
CCTGTTCCGAGGAGCGCGCC

Downstream 100 bases:

>100_bases
AGCCATGAAGGTCGCCATCATCGGCGGCGGCATCATCGGGCTCGCCCTGGGCTGGCGCCTCGCGCAGCGCGGCGCCGAGA
CCACGGTGTTCGAGCGCGAT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MNVQTKPASVTTGPIAGSRKVFTSPEGRPDIAVPFREVDLHPSAGEAPFRLYDTSGPFTDPHFSLDLDNGLPLVRGAWIE
RRGFDAVAGRAVKPEDNGFAAADRLVPPCPAGRSVFAGKDGALVTQYEYARAGIVTEEMIYVAHRENLGRAKAAEGAAAR
RADGERFGAAIPEFVTPEFVRSEIAAGRAIIPANINHPELEPVIIGRNFLVKINANIGNSAVTSGAAEEVEKLVWAIRWG
ADTVMDLSTGRNIHNIRSWIMRNSPVPIGTVPIYQALEKVGGDPDKLDWEVFRDTLIEQAEQGVDYFTIHAGVRLAHVPL
TAKRVTGIVSRGGSIMARWCLAHHRESFLYEKFDEICDIMRKYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIA
WAKGCQVMIEGPGHVPMHKIKENMEKQLEVCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLG
LPNRDDVKTGVITYRLAAHAADLAKGHPAAKLRDDAVSRARFEFRWEDQFNLGLDPDTARAFHDETMPKEAHKVAHFCSM
CGPKFCSMKITQDIRDEVAREEGLAQKAAEFRDGGSRIYVEDQAAE

Sequences:

>Translated_606_residues
MNVQTKPASVTTGPIAGSRKVFTSPEGRPDIAVPFREVDLHPSAGEAPFRLYDTSGPFTDPHFSLDLDNGLPLVRGAWIE
RRGFDAVAGRAVKPEDNGFAAADRLVPPCPAGRSVFAGKDGALVTQYEYARAGIVTEEMIYVAHRENLGRAKAAEGAAAR
RADGERFGAAIPEFVTPEFVRSEIAAGRAIIPANINHPELEPVIIGRNFLVKINANIGNSAVTSGAAEEVEKLVWAIRWG
ADTVMDLSTGRNIHNIRSWIMRNSPVPIGTVPIYQALEKVGGDPDKLDWEVFRDTLIEQAEQGVDYFTIHAGVRLAHVPL
TAKRVTGIVSRGGSIMARWCLAHHRESFLYEKFDEICDIMRKYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIA
WAKGCQVMIEGPGHVPMHKIKENMEKQLEVCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLG
LPNRDDVKTGVITYRLAAHAADLAKGHPAAKLRDDAVSRARFEFRWEDQFNLGLDPDTARAFHDETMPKEAHKVAHFCSM
CGPKFCSMKITQDIRDEVAREEGLAQKAAEFRDGGSRIYVEDQAAE
>Mature_606_residues
MNVQTKPASVTTGPIAGSRKVFTSPEGRPDIAVPFREVDLHPSAGEAPFRLYDTSGPFTDPHFSLDLDNGLPLVRGAWIE
RRGFDAVAGRAVKPEDNGFAAADRLVPPCPAGRSVFAGKDGALVTQYEYARAGIVTEEMIYVAHRENLGRAKAAEGAAAR
RADGERFGAAIPEFVTPEFVRSEIAAGRAIIPANINHPELEPVIIGRNFLVKINANIGNSAVTSGAAEEVEKLVWAIRWG
ADTVMDLSTGRNIHNIRSWIMRNSPVPIGTVPIYQALEKVGGDPDKLDWEVFRDTLIEQAEQGVDYFTIHAGVRLAHVPL
TAKRVTGIVSRGGSIMARWCLAHHRESFLYEKFDEICDIMRKYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIA
WAKGCQVMIEGPGHVPMHKIKENMEKQLEVCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLG
LPNRDDVKTGVITYRLAAHAADLAKGHPAAKLRDDAVSRARFEFRWEDQFNLGLDPDTARAFHDETMPKEAHKVAHFCSM
CGPKFCSMKITQDIRDEVAREEGLAQKAAEFRDGGSRIYVEDQAAE

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=611, Percent_Identity=62.847790507365, Blast_Score=777, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 66368; Mature: 66368

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVQTKPASVTTGPIAGSRKVFTSPEGRPDIAVPFREVDLHPSAGEAPFRLYDTSGPFTD
CCCCCCCCCEECCCCCCCCEEEECCCCCCCEECCCEEEECCCCCCCCCEEEEECCCCCCC
PHFSLDLDNGLPLVRGAWIERRGFDAVAGRAVKPEDNGFAAADRLVPPCPAGRSVFAGKD
CCEEEECCCCCCCHHCCHHHHCCCHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCEECCCC
GALVTQYEYARAGIVTEEMIYVAHRENLGRAKAAEGAAARRADGERFGAAIPEFVTPEFV
CCEEEEEHHHHHCCCHHHHHHHHHHCCCCCHHHHCCCHHHCCCCHHHHCCCCCCCCHHHH
RSEIAAGRAIIPANINHPELEPVIIGRNFLVKINANIGNSAVTSGAAEEVEKLVWAIRWG
HHHHHCCCEEEECCCCCCCCCEEEECCEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHCC
ADTVMDLSTGRNIHNIRSWIMRNSPVPIGTVPIYQALEKVGGDPDKLDWEVFRDTLIEQA
CCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
EQGVDYFTIHAGVRLAHVPLTAKRVTGIVSRGGSIMARWCLAHHRESFLYEKFDEICDIM
HCCCCEEEEECCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIAWAKGCQVMIEGPGHVPMHKI
HHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHH
KENMEKQLEVCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLG
HHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCC
LPNRDDVKTGVITYRLAAHAADLAKGHPAAKLRDDAVSRARFEFRWEDQFNLGLDPDTAR
CCCCCCCCHHHEEHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEECCCCCCCCCCCHHH
AFHDETMPKEAHKVAHFCSMCGPKFCSMKITQDIRDEVAREEGLAQKAAEFRDGGSRIYV
HHHHHCCCHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
EDQAAE
ECCCCC
>Mature Secondary Structure
MNVQTKPASVTTGPIAGSRKVFTSPEGRPDIAVPFREVDLHPSAGEAPFRLYDTSGPFTD
CCCCCCCCCEECCCCCCCCEEEECCCCCCCEECCCEEEECCCCCCCCCEEEEECCCCCCC
PHFSLDLDNGLPLVRGAWIERRGFDAVAGRAVKPEDNGFAAADRLVPPCPAGRSVFAGKD
CCEEEECCCCCCCHHCCHHHHCCCHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCEECCCC
GALVTQYEYARAGIVTEEMIYVAHRENLGRAKAAEGAAARRADGERFGAAIPEFVTPEFV
CCEEEEEHHHHHCCCHHHHHHHHHHCCCCCHHHHCCCHHHCCCCHHHHCCCCCCCCHHHH
RSEIAAGRAIIPANINHPELEPVIIGRNFLVKINANIGNSAVTSGAAEEVEKLVWAIRWG
HHHHHCCCEEEECCCCCCCCCEEEECCEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHCC
ADTVMDLSTGRNIHNIRSWIMRNSPVPIGTVPIYQALEKVGGDPDKLDWEVFRDTLIEQA
CCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
EQGVDYFTIHAGVRLAHVPLTAKRVTGIVSRGGSIMARWCLAHHRESFLYEKFDEICDIM
HCCCCEEEEECCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIAWAKGCQVMIEGPGHVPMHKI
HHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHH
KENMEKQLEVCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLG
HHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCC
LPNRDDVKTGVITYRLAAHAADLAKGHPAAKLRDDAVSRARFEFRWEDQFNLGLDPDTAR
CCCCCCCCHHHEEHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEECCCCCCCCCCCHHH
AFHDETMPKEAHKVAHFCSMCGPKFCSMKITQDIRDEVAREEGLAQKAAEFRDGGSRIYV
HHHHHCCCHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
EDQAAE
ECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA